RetrogeneDB ID: retro_cfam_366

Retrocopy location

• Organism: Dog (Canis familiaris)
• Coordinates: 10:58281545..58282036(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: CFL1
• Ensembl ID: ENSCAFG00000024955
• Aliases: None
• Description: cofilin 1 (non-muscle) [Source:HGNC Symbol;Acc:1874]

Sequence

>retro_cfam_366
GCCTCTGGTATAGCTGTCTCTGATGGCGTCATCAAAGTGTTGGATGACATGAAGGTGTGTAAGTCGTCAACACCAGAGGA
TGTGAAGAAGCGCAAGAAGGCAGTGTTCTTCTGCCTGAACAAGGACAAGAACGTTATTTGGAGGAAGGCAAGGTGATCCT
GGTAGGTTATCTGGACCAGACTGTAGAAGACAGCACCTATGCCATCTGTCCAGATAGTCCAGCTAGGGATGCCACTATGC
CCCCTATGATGTAATTTCAAAACCAAGGAGACAAGGAGGAGGATCTGGTGTTTATCTTCTGGGCACCTGGGTCTGAAGCA
TTTAAGAGCACAGTGATCTAAGCCAGTTCCAAGCACATCATCAAGAAAGGGCTGACAGGGATCCAGCATAGATTATAACC
AAACTACTATGAAGAGGTCAAGGACCACTGTACCCTGGCAGAGTAGCTGAAGGGGGTTAGTGCCAACATCTTTCTGGAGG
ACAAGCCTTTG

Alignment

ORF - retro_cfam_366 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 64.71 %
• Parental protein coverage: 99.4 %
• Number of stop codons detected: 3
• Number of frameshifts detected: 4

Retrocopy - Parental Gene Alignment:

Parental  ASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNII-LEEGKEILVGDVGQTVDDPYAT
          ASG.AVSDGVIKV..DMKV.KSSTPE.VKKRKKAV.FCL..DK.N.I.LEEGK.ILVG...QTV.D....
Retrocopy ASGIAVSDGVIKVLDDMKVCKSSTPEDVKKRKKAVFFCLNKDK-NVI<LEEGKVILVGYLDQTVEDSTYA

Parental  FVKMLPDKD-CRYALYDA-TYETKES-KKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQA
          .....P..D.C.YA.YD.....TKE..K.EDLVFIFWAP.S...KS..I.ASSK..IKK.LTGI.H.L..
Retrocopy ICPDSPARD<CHYAPYDV<NFKTKET<KEEDLVFIFWAPGSEAFKSTVI*ASSKHIIKKGLTGIQHRL*P

Parental  NCYEEVKDRCTLAEKLGG-SAVISLEGKPL
          N.YEEVKD.CTLAE.L.G.SA.I.LE.KPL
Retrocopy NYYEEVKDHCTLAE*LKGVSANIFLEDKPL

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012049_cerebellum	0.04 RPM	541.62 RPM
SRP017611_brain	0.00 RPM	280.18 RPM
SRP017611_kidney	0.00 RPM	241.23 RPM
SRP017611_liver	0.00 RPM	54.02 RPM

ChIP-Seq

Canis familiaris was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_cfam_366 retrocopy.

TSS

Canis familiaris was not studied using FANTOM5 data.

Lab

retro_cfam_366 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_cfam_366 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 23 parental genes, and 83 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000017334	2 retrocopies	retro_amel_583, retro_amel_816,
Bos taurus	ENSBTAG00000021455	1 retrocopy	retro_btau_1382,
Canis familiaris	ENSCAFG00000024955	6 retrocopies	retro_cfam_1544, retro_cfam_366 , retro_cfam_660, retro_cfam_67, retro_cfam_943, retro_cfam_964,
Canis familiaris	ENSCAFG00000032562	1 retrocopy	retro_cfam_1006,
Callithrix jacchus	ENSCJAG00000001916	1 retrocopy	retro_cjac_347,
Equus caballus	ENSECAG00000019900	1 retrocopy	retro_ecab_442,
Felis catus	ENSFCAG00000013272	3 retrocopies	retro_fcat_1456, retro_fcat_303, retro_fcat_991,
Homo sapiens	ENSG00000172757	6 retrocopies	retro_hsap_2617, retro_hsap_287, retro_hsap_3333, retro_hsap_336, retro_hsap_384, retro_hsap_400,
Gorilla gorilla	ENSGGOG00000023183	5 retrocopies	retro_ggor_1832, retro_ggor_326, retro_ggor_357, retro_ggor_389, retro_ggor_397,
Microcebus murinus	ENSMICG00000012763	1 retrocopy	retro_mmur_703,
Myotis lucifugus	ENSMLUG00000024685	6 retrocopies	retro_mluc_1905, retro_mluc_2114, retro_mluc_216, retro_mluc_2218, retro_mluc_455, retro_mluc_507,
Macaca mulatta	ENSMMUG00000019016	5 retrocopies	retro_mmul_316, retro_mmul_493, retro_mmul_556, retro_mmul_667, retro_mmul_705,
Mustela putorius furo	ENSMPUG00000011502	2 retrocopies	retro_mputfur_1485, retro_mputfur_575,
Mus musculus	ENSMUSG00000056201	6 retrocopies	retro_mmus_1729, retro_mmus_2103, retro_mmus_3127, retro_mmus_3556, retro_mmus_719, retro_mmus_970,
Nomascus leucogenys	ENSNLEG00000005852	5 retrocopies	retro_nleu_139, retro_nleu_1494, retro_nleu_1571, retro_nleu_1777, retro_nleu_636,
Oryctolagus cuniculus	ENSOCUG00000025604	4 retrocopies	retro_ocun_1123, retro_ocun_1390, retro_ocun_324, retro_ocun_478,
Otolemur garnettii	ENSOGAG00000016503	8 retrocopies	retro_ogar_1442, retro_ogar_1581, retro_ogar_1648, retro_ogar_2192, retro_ogar_302, retro_ogar_405, retro_ogar_453, retro_ogar_828,
Pongo abelii	ENSPPYG00000003047	5 retrocopies	retro_pabe_1732, retro_pabe_2747, retro_pabe_316, retro_pabe_325, retro_pabe_386,
Pan troglodytes	ENSPTRG00000003895	5 retrocopies	retro_ptro_1764, retro_ptro_2146, retro_ptro_236, retro_ptro_298, retro_ptro_306,
Rattus norvegicus	ENSRNOG00000020660	2 retrocopies	retro_rnor_1354, retro_rnor_693,
Ictidomys tridecemlineatus	ENSSTOG00000027968	4 retrocopies	retro_itri_144, retro_itri_1456, retro_itri_1734, retro_itri_685,
Tupaia belangeri	ENSTBEG00000016751	2 retrocopies	retro_tbel_153, retro_tbel_3085,
Tursiops truncatus	ENSTTRG00000016931	2 retrocopies	retro_ttru_1256, retro_ttru_598,