RetrogeneDB ID: retro_mmus_2314

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 3:152071243..152071740(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Pebp1
• Ensembl ID: ENSMUSG00000032959
• Aliases: Pebp1, HCNP, Pbp, Pbp1, Pbpr, Rkip
• Description: phosphatidylethanolamine binding protein 1 [Source:MGI Symbol;Acc:MGI:1344408]

Sequence

>retro_mmus_2314
CGGCACACTCTGCGAGCTGCCTATGCAGGGGTGGAGGTGGGTGAGCTGGGCAAAGTGCAGAGACCCACCCAGGCTAAGAA
TAGTCCCAGTAGCATTTCATGGGATGGCCTTGATCCAGGGAAACTCTACATCCTGGTCCTTACAGACTCGGATGCTTTCA
GCAAGAAGGGCCCTACTTTCAGGAAATGGCACCACTTTTTGGTCGTCAACATGAAGGACAATGGCATTAGCAGTGTCACG
GTCCTCTCAGATTACATGGGCTCTGGGCCTCCCAGTGGCACAGGCCTCCAACGCTATGTCTGGCTGGTGTATGAGTAGGA
AAAGCCACTGAACTGTGATGAGCCCATCCTCAGCAACAGGTCTGGAGATCACCGCAGCAACTTGGAGGTGGCGCCTTCTG
CAAGAAGTACTACCTGGAAGCCCTGGTGGCGGACATGTGTTGCTAGGAAGAGTGGGACGACTCTGCGCTCAAGCTGTAAG
AGCAGCTGTCTGGAAAG

Alignment

ORF - retro_mmus_2314 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 71.26 %
• Parental protein coverage: 88.77 %
• Number of stop codons detected: 3
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  QHALRVDYAGVTVDELGKVLTPTQVMNRPSSISWDGLDPGKLYTLVLTDPDAPSRKDPKFREWHHFLVVN
          .H.LR..YAGV.V.ELGKV..PTQ..N.PSSISWDGLDPGKLY.LVLTD.DA.S.K.P.FR.WHHFLVVN
Retrocopy RHTLRAAYAGVEVGELGKVQRPTQAKNSPSSISWDGLDPGKLYILVLTDSDAFSKKGPTFRKWHHFLVVN

Parental  MKGNDISSGTVLSDYVGSGPPSGTGLHRYVWLVYEQEQPLSCDEPILSNKSGDNRGKFKVE-TFRKKYNL
          MK.N.ISS.TVLSDY.GSGPPSGTGL.RYVWLVYE.E.PL.CDEPILSN.SGD.R....V...F.KKY.L
Retrocopy MKDNGISSVTVLSDYMGSGPPSGTGLQRYVWLVYE*EKPLNCDEPILSNRSGDHRSNLEVA<AFCKKYYL

Parental  GAPVAGTCYQAEWDDYVPKLYEQLSGK
          .A.VA..C...EWDD...KL.EQLSGK
Retrocopy EALVADMCC*EEWDDSALKL*EQLSGK

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	66.21 RPM
SRP007412_cerebellum	0.00 RPM	54.32 RPM
SRP007412_heart	0.00 RPM	46.63 RPM
SRP007412_kidney	0.00 RPM	53.36 RPM
SRP007412_liver	0.00 RPM	45.36 RPM
SRP007412_testis	0.00 RPM	301.74 RPM

ChIP-Seq

RNA Polymerase II activity near the 5' end of retro_mmus_2314 was not detected

EST

No EST(s) were mapped for retro_mmus_2314 retrocopy.

TSS

No TSS is located nearby retro_mmus_2314 retrocopy 5' end.

Lab

retro_mmus_2314 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_2314 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 21 parental genes, and 52 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000016143	2 retrocopies	retro_amel_1355, retro_amel_754,
Bos taurus	ENSBTAG00000018115	2 retrocopies	retro_btau_1183, retro_btau_705,
Canis familiaris	ENSCAFG00000009910	1 retrocopy	retro_cfam_526,
Callithrix jacchus	ENSCJAG00000009314	2 retrocopies	retro_cjac_1410, retro_cjac_818,
Erinaceus europaeus	ENSEEUG00000003689	1 retrocopy	retro_eeur_292,
Felis catus	ENSFCAG00000002277	3 retrocopies	retro_fcat_1317, retro_fcat_1360, retro_fcat_299,
Homo sapiens	ENSG00000089220	3 retrocopies	retro_hsap_1456, retro_hsap_2288, retro_hsap_492,
Gorilla gorilla	ENSGGOG00000013281	3 retrocopies	retro_ggor_1121, retro_ggor_1701, retro_ggor_461,
Myotis lucifugus	ENSMLUG00000002259	7 retrocopies	retro_mluc_103, retro_mluc_1612, retro_mluc_1742, retro_mluc_2357, retro_mluc_2373, retro_mluc_984, retro_mluc_998,
Macaca mulatta	ENSMMUG00000005098	4 retrocopies	retro_mmul_1621, retro_mmul_2272, retro_mmul_328, retro_mmul_442,
Mustela putorius furo	ENSMPUG00000002861	3 retrocopies	retro_mputfur_1228, retro_mputfur_1361, retro_mputfur_223,
Mus musculus	ENSMUSG00000032959	4 retrocopies	retro_mmus_138, retro_mmus_1857, retro_mmus_2314 , retro_mmus_542,
Nomascus leucogenys	ENSNLEG00000000322	1 retrocopy	retro_nleu_1966,
Oryctolagus cuniculus	ENSOCUG00000008395	2 retrocopies	retro_ocun_1329, retro_ocun_1900,
Otolemur garnettii	ENSOGAG00000001740	1 retrocopy	retro_ogar_2750,
Pongo abelii	ENSPPYG00000005011	2 retrocopies	retro_pabe_1202, retro_pabe_241,
Pan troglodytes	ENSPTRG00000005512	3 retrocopies	retro_ptro_1621, retro_ptro_371, retro_ptro_992,
Rattus norvegicus	ENSRNOG00000001136	3 retrocopies	retro_rnor_1223, retro_rnor_46, retro_rnor_541,
Ictidomys tridecemlineatus	ENSSTOG00000004085	2 retrocopies	retro_itri_1603, retro_itri_961,
Tupaia belangeri	ENSTBEG00000000177	2 retrocopies	retro_tbel_272, retro_tbel_2728,
Tursiops truncatus	ENSTTRG00000000658	1 retrocopy	retro_ttru_1164,