RetrogeneDB ID: retro_ecab_110

Retrocopy location

• Organism: Horse (Equus caballus)
• Coordinates: 7:13734100..13735303(+)
• Located in intron of: ENSECAG00000024746

Retrocopy information

• Ensembl ID: ENSECAG00000005520
• Aliases: None
• Status: KNOWN_PROTEIN_CODING

Parental gene information

• Parental gene symbol: DDI2
• Ensembl ID: ENSECAG00000000762
• Aliases: None
• Description: DNA-damage inducible 1 homolog 2 (S. cerevisiae) [Source:HGNC Symbol;Acc:24578]

Sequence

>retro_ecab_110
ATGCTGGTCACTGTGTACTGCGTGCGGAGGGACCTCTCCGAAGTAACCTTCTCCCTCCAGGTCAGCCCTGACTTCGAGCT
CCACAACTTTCGGGTCCTCTGTGAGCTTGAGTCCGGTGTTCCTGCTGAAGAGACCCAGATCATCTACATGGAGCGACTCC
TCACTGACGACCACTGTTCCCTAGGCTCCTATGGCCTCAAAGACGGCGATGTCGTTGTTTTACTTCAGAAGGAGAATGTG
GGACCTTGGCATCCCGGACGGACATCAAGCCTCCCTCGAATTGATTTCAGTGGGATAGCCGAGCCTGGGACATCCAGCTC
CCGGCAGCAGCACCATCAGCAGCAGCGTGCACCATCAGCCCAGAAGGCCCATGGCCTGGACTCTGGAGAGAAGATGAGGT
CTGCTCAAGGTCTGGACAGCCCCGCCTTGATCCGAAGCATGCTGCTGTCCAATCCCCATGATCTGTCCCTGCTGAAGGAG
CGCAACCCCACCTTGGCGGAAGCCCTGCTCAGCGGAAACCTTGAGACGTTTTCTCAGGTCCTGATGGAGCAGCAAAGGGA
AAGGGCCCTGAGAGAGCAAGAGAGGCTTCGCCTCTGTTCAGCTGATCCATTTGATTTGGAAGCTCAGGCCAAAATAGAAG
AAGAAATCCGGCAGCAGAACATTGAGGAAAACATGAACATAGCAATGGAAGAGGCTCCAGAGAGTTTCGGACAAGTGGCC
ATGCTCTATATCAACTGCAAAGTGAACGGACATCCTTTGAAGGCTTTTGTTGACTCAGGAGCCCAAATGACCATTATGAG
CCAAGCTTGTGCGGAGAGATGTAATATAATACGGCTGGTTGACCGACGGTGGGCTGGGATTGCTAAAGGAGTAGGCATGC
AGAGAATTATTGGCCGAATTCATCTGGCTCAGATTCAAATCGAAGGTGATTTCTTACAATGCTCTTTCTCTATACTCGAG
GAGCAGCCCATGGACATGCTTCTAGGGCTAGATATGCTCAGGAGACATCAGTGTTCCATTGACTTGAAGAAAAACGTGCT
GGTGATCGGCACCACTGGCACACAGACTCACTTTCTTCCTGAGGGAGAGTTACCTCCATGTGCTAAGCTGGTAAGTGGTG
CTGGGCAAGAAGAGTCTTCAAACAAGGAAGCAGCAAATACTACTAAACATTCAGTCATGGATTCAGGAAGAAAAAAGCAT
TGA

Alignment

ORF - retro_ecab_110 Open Reading Frame is conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 79.12 %
• Parental protein coverage: 77.34 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 0

Retrocopy - Parental Gene Alignment:

Parental  RQRQPAGVQQSHSSPGEIASSPQGLDNPALLRDMLLANPHELSLLKERNPPLADALLSGDLEKFSRVLVE
          .QR.P..........GE...S.QGLD.PAL.R.MLL.NPH.LSLLKERNP.LA.ALLSG.LE.FS.VL.E
Retrocopy QQRAPSAQKAHGLDSGEKMRSAQGLDSPALIRSMLLSNPHDLSLLKERNPTLAEALLSGNLETFSQVLME

Parental  QQQDRARREQERIRLFSADPFDLEAQAKIEEDIRQQNIEENMTIAMEEAPESFGQVVMLYINCKVNGHPV
          QQ..RA.REQER.RL.SADPFDLEAQAKIEE.IRQQNIEENM.IAMEEAPESFGQV.MLYINCKVNGHP.
Retrocopy QQRERALREQERLRLCSADPFDLEAQAKIEEEIRQQNIEENMNIAMEEAPESFGQVAMLYINCKVNGHPL

Parental  KAFVDSGAQMTIMSQACAERCNIMRLVDRRWAGIAKGVGTQKIIGRVHLAQVQIEGDFLACSFSILEEQP
          KAFVDSGAQMTIMSQACAERCNI.RLVDRRWAGIAKGVG.Q.IIGR.HLAQ.QIEGDFL.CSFSILEEQP
Retrocopy KAFVDSGAQMTIMSQACAERCNIIRLVDRRWAGIAKGVGMQRIIGRIHLAQIQIEGDFLQCSFSILEEQP

Parental  MDMLLGLDMLKRHQCSIDLKKNVLVIGTTGSQTTFLPEGELPECARLAYGAGREDVRPEEFAD
          MDMLLGLDML.RHQCSIDLKKNVLVIGTTG.QT.FLPEGELP.CA.L..GAG.E.....E.A.
Retrocopy MDMLLGLDMLRRHQCSIDLKKNVLVIGTTGTQTHFLPEGELPPCAKLVSGAGQEESSNKEAAN

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP021940_articular_cartilage	0.00 RPM	4.96 RPM
SRP021940_cerebellum	0.05 RPM	3.84 RPM
SRP021940_embryo	0.08 RPM	11.33 RPM
SRP021940_placental_villous	0.19 RPM	5.77 RPM
SRP021940_synovial_membrane	0.19 RPM	7.11 RPM
SRP021940_testis	167.15 RPM	1.79 RPM

ChIP-Seq

Equus caballus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ecab_110 retrocopy.

TSS

Equus caballus was not studied using FANTOM5 data.

Lab

retro_ecab_110 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ecab_110 has 11 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_96
Pan troglodytes	retro_ptro_570
Gorilla gorilla	retro_ggor_143
Pongo abelii	retro_pabe_158
Macaca mulatta	retro_mmul_231
Callithrix jacchus	retro_cjac_196
Mus musculus	retro_mmus_67
Oryctolagus cuniculus	retro_ocun_249
Bos taurus	retro_btau_15
Sus scrofa	retro_sscr_6
Canis familiaris	retro_cfam_193

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Parental genes homology:

Parental genes homology involve 24 parental genes, and 25 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000011873	1 retrocopy	retro_amel_10,
Bos taurus	ENSBTAG00000018718	2 retrocopies	retro_btau_15, retro_btau_650,
Canis familiaris	ENSCAFG00000016220	1 retrocopy	retro_cfam_193,
Callithrix jacchus	ENSCJAG00000008625	1 retrocopy	retro_cjac_196,
Cavia porcellus	ENSCPOG00000020885	1 retrocopy	retro_cpor_88,
Equus caballus	ENSECAG00000000762	1 retrocopy	retro_ecab_110 ,
Homo sapiens	ENSG00000197312	1 retrocopy	retro_hsap_96,
Gorilla gorilla	ENSGGOG00000013537	1 retrocopy	retro_ggor_143,
Macropus eugenii	ENSMEUG00000006148	1 retrocopy	retro_meug_1364,
Myotis lucifugus	ENSMLUG00000030669	1 retrocopy	retro_mluc_68,
Macaca mulatta	ENSMMUG00000019912	1 retrocopy	retro_mmul_231,
Monodelphis domestica	ENSMODG00000003515	1 retrocopy	retro_mdom_282,
Mustela putorius furo	ENSMPUG00000016332	1 retrocopy	retro_mputfur_1509,
Mus musculus	ENSMUSG00000078515	1 retrocopy	retro_mmus_67,
Oryctolagus cuniculus	ENSOCUG00000023891	1 retrocopy	retro_ocun_249,
Otolemur garnettii	ENSOGAG00000027709	1 retrocopy	retro_ogar_4,
Ochotona princeps	ENSOPRG00000018448	1 retrocopy	retro_opri_4,
Pongo abelii	ENSPPYG00000001841	1 retrocopy	retro_pabe_158,
Pan troglodytes	ENSPTRG00000024059	1 retrocopy	retro_ptro_570,
Pteropus vampyrus	ENSPVAG00000005084	1 retrocopy	retro_pvam_420,
Rattus norvegicus	ENSRNOG00000012274	1 retrocopy	retro_rnor_2606,
Sus scrofa	ENSSSCG00000003462	1 retrocopy	retro_sscr_6,
Ictidomys tridecemlineatus	ENSSTOG00000019447	1 retrocopy	retro_itri_93,
Tarsius syrichta	ENSTSYG00000002522	1 retrocopy	retro_tsyr_1120,