RetrogeneDB ID: retro_ptro_776

Retrocopy location

• Organism: Chimpanzee (Pan troglodytes)
• Coordinates: 12:87938208..87939488(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: MKRN1
• Ensembl ID: ENSPTRG00000019766
• Aliases: None
• Description: makorin ring finger protein 1 [Source:HGNC Symbol;Acc:7112]

Sequence

>retro_ptro_776
ATGGCGGAGGCTGCAGCTCTCAGACCAACAGCCACAACATCAGGGGCGGGAGTGGCGGCAGCGGTGGCGGCAGCAGCGGC
CTCCCCTACCAGGATCCCCACCGTCACCATTCTGTCCCCAGGGGGCAGGCGAGGGGGCCGCGGCAGCGATGGCAGCGGCG
GCAGCTGGACTAAACATGTCACCTGCAGGTATTTTATGCATGGGGTTTATAAGGAAGGAGATAACTGTTGCAAGTTGCAT
GACCTCTCTGACAGTCCGTATAATGTAGTGTACAAGAGTTTGCAGCGAGGGTACTGTATTTATGGAGACTGCTGCAGATA
TGAACATAGCAAGCCATTGAAACAGAAAGAAGCAACTGCTGTAGAGCTAACTACAAAGTCGTCCCTTGCTGCTTCCTCAA
GTCTCTCATGGATAGTCGGACCACTTGTTGAAATGAATACGGGTGAAGCTGAGTCAAGAAATTCAAACTTTGTAACTGTA
GGAGCAGGTTCAGAGGGCTGGGTGAATGCTGTTGAGTTTGTTCCTGGGCAACCCTACTGTGGCCGCACTGCGCCTTCCTG
CACTGAAGCACCCCTGCAGGGCTCAGTGACCAAGGAAGAATCAGAGAAAGAGCAAACTGCCGTGGAAACAAAGAAGCAGC
TGTGCCCCTATGCTGCAGTGGGAGAGTGCCGATATCGGGAGAACTTGTGTGTATACGGAGATTCGTGTGACATGTGTGGC
TGCAGGTCCTGCATCTGATGGATGCTGCCCAGAGATCACAGCATATAAAATCGTGCTTTGAGGCCCATGAGAAAGACATG
GAGCTCTCATTTGCTGTGCAGCGCAGTGAGGAAATGGTGTGTGAGAGCTGCATGGAGATGATCTATGAGAAAGCCAACCC
CAGAGAGCACCGCTTCGGGATCCTCTTCAACTGCAACCACACCTACTGTCTTAAGTGCATTCGCAAGTGGAGGAGTGCTA
AGCAGTTTGAGAGCAAGATCATAAAGTCCTGCCCAGAATGTCGGATCACATCTAACTTTGTCATTCCAAGTGAGTACTGT
GTGGAGGAGAAAGAAGAGAAGCAGAAACTCATTCTGAAATACAAGGAGGCAATGAGCAACAAGGCATGCAGGTATTTTGA
TGAAGGACATGGGAGCTGTCCGTTTGGAGGGAACAGTTTTTATAAGCATGTGTACCCTGATGGCCGTAGAGAGGAGCCAC
AGAGACAGAAAGTGGGAACATCAAGCAGATACCCGGCCCAATGAAGGAACCACTTCTGGGAACTCATTGAGGAAAGAGAA

Alignment

ORF - retro_ptro_776 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 87.67 %
• Parental protein coverage: 88.59 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  MAEAAAPGTTATTSGAGAAAATAAAA-SPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVTCRYFMHGVC
          MAEAAA...TATTSGAG.AAA.AAAA.SPT.IPTVT..S.G...GG.GSDGSGG.WTK.VTCRYFMHGV.
Retrocopy MAEAAALRPTATTSGAGVAAAVAAAAASPTRIPTVTILSPGGRRGGRGSDGSGGSWTKHVTCRYFMHGVY

Parental  KEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATELTTKSSLAASSSLSSIVG
          KEGDNC...HDLSDSPY.VV.K..QRGYCIYGD.CRYEHSKPLKQ.EATA.ELTTKSSLAASSSLS.IVG
Retrocopy KEGDNCCKLHDLSDSPYNVVYKSLQRGYCIYGDCCRYEHSKPLKQKEATAVELTTKSSLAASSSLSWIVG

Parental  PLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVET
          PLVEMNTGEAESRNSNF.TVGAGSE.WVNA.EFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVET
Retrocopy PLVEMNTGEAESRNSNFVTVGAGSEGWVNAVEFVPGQPYCGRTAPSCTEAPLQGSVTKEESEKEQTAVET

Parental  KKQLCPYAAVGECRYGEN-CVYIHGDSCDMCG-LQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSK
          KKQLCPYAAVGECRY.EN.CVY..GDSCDMCG.LQVLH.MDAAQRSQHIKSC.EAHEKDMELSFAVQRS.
Retrocopy KKQLCPYAAVGECRYRENLCVY--GDSCDMCG<LQVLHLMDAAQRSQHIKSCFEAHEKDMELSFAVQRSE

Parental  DMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYW
          .MVC..CME..YEKANP.E.RFGIL.NCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEY.
Retrocopy EMVCESCMEMIYEKANPREHRFGILFNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYC

Parental  VEEKEEKQKLILKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRN
          VEEKEEKQKLILKYKEAMSNKACRYFDEG.GSCPFGGN.FYKH.YPDGRREEPQRQKVGTSSRY.AQ.RN
Retrocopy VEEKEEKQKLILKYKEAMSNKACRYFDEGHGSCPFGGNSFYKHVYPDGRREEPQRQKVGTSSRYPAQ*RN

Parental  HFWELIEERE
          HFWELIEERE
Retrocopy HFWELIEERE

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain_prefrontal_cortex	0.02 RPM	62.44 RPM
SRP007412_cerebellum	0.07 RPM	123.61 RPM
SRP007412_heart	0.00 RPM	87.53 RPM
SRP007412_kidney	0.03 RPM	90.01 RPM
SRP007412_liver	0.00 RPM	52.73 RPM
SRP007412_testis	4.74 RPM	1105.68 RPM

ChIP-Seq

Pan troglodytes was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ptro_776 retrocopy.

TSS

Pan troglodytes was not studied using FANTOM5 data.

Lab

retro_ptro_776 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ptro_776 has 2 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_1140
Pongo abelii	retro_pabe_947

---

Parental genes homology:

Parental genes homology involve 19 parental genes, and 47 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000008306	2 retrocopies	retro_btau_401, retro_btau_943,
Canis familiaris	ENSCAFG00000003953	2 retrocopies	retro_cfam_2157, retro_cfam_253,
Choloepus hoffmanni	ENSCHOG00000011015	3 retrocopies	retro_chof_173, retro_chof_2357, retro_chof_89,
Callithrix jacchus	ENSCJAG00000013474	2 retrocopies	retro_cjac_210, retro_cjac_2991,
Cavia porcellus	ENSCPOG00000000948	1 retrocopy	retro_cpor_109,
Dasypus novemcinctus	ENSDNOG00000019564	2 retrocopies	retro_dnov_1011, retro_dnov_388,
Felis catus	ENSFCAG00000024942	1 retrocopy	retro_fcat_67,
Homo sapiens	ENSG00000133606	4 retrocopies	retro_hsap_1140, retro_hsap_4197, retro_hsap_4676, retro_hsap_4859,
Gorilla gorilla	ENSGGOG00000023977	4 retrocopies	retro_ggor_119, retro_ggor_2813, retro_ggor_3031, retro_ggor_381,
Microcebus murinus	ENSMICG00000002114	1 retrocopy	retro_mmur_1184,
Macaca mulatta	ENSMMUG00000010420	3 retrocopies	retro_mmul_191, retro_mmul_2597, retro_mmul_76,
Mus musculus	ENSMUSG00000029922	1 retrocopy	retro_mmus_2663,
Nomascus leucogenys	ENSNLEG00000001921	4 retrocopies	retro_nleu_1027, retro_nleu_2167, retro_nleu_33, retro_nleu_939,
Otolemur garnettii	ENSOGAG00000009877	2 retrocopies	retro_ogar_3468, retro_ogar_424,
Pongo abelii	ENSPPYG00000018057	3 retrocopies	retro_pabe_3435, retro_pabe_3776, retro_pabe_947,
Pan troglodytes	ENSPTRG00000019766	5 retrocopies	retro_ptro_2849, retro_ptro_3106, retro_ptro_3234, retro_ptro_75, retro_ptro_776 ,
Sus scrofa	ENSSSCG00000016498	2 retrocopies	retro_sscr_43, retro_sscr_637,
Tarsius syrichta	ENSTSYG00000009996	3 retrocopies	retro_tsyr_1256, retro_tsyr_1525, retro_tsyr_508,
Tursiops truncatus	ENSTTRG00000009528	2 retrocopies	retro_ttru_1064, retro_ttru_1128,