RetrogeneDB ID: retro_dnov_1426

Retrocopy location

• Organism: Armadillo (Dasypus novemcinctus)
• Coordinates: scaffold_20194:15178..15660(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: RAB28
• Ensembl ID: ENSDNOG00000007750
• Aliases: None
• Description: RAB28, member RAS oncogene family [Source:HGNC Symbol;Acc:9768]

Sequence

>retro_dnov_1426
ACTGAAGAAGAGAGCCAGGACCTGCAATTGAAAATCGTCGTGCTGGGGAACGGCACTTCTGGGAGGACCTCCTTAGCTAT
GTGTTTTGCTCATGAAACTTTTGGGAAACACAACAAACAAACTATAGGACTAAATTTCATTTTAAGAAGGATAATATTGC
CAGGGAAGTTGAATGTTACTCAAGTTTGGGATATAGGAGGGCAGACAATAAGAGACAAGATTTGGATAAATATATCTATG
GAGCACAAGGAATCCTCTTGGTATGTGATATTACAAATTATCAAAGCTCTGAGACTTTAGAAGATTGGTACACTATAGTG
AAGAAAGTAAGCGAGGAATTCAAAACTCACCCACTGCTTGCCTTGGTGGGCAATAAAATTGATTTGGAGCATAGGTGTAG
AGTAAAACCTGAAAAACACTTAAGGTTTTGCCAGGAAAATGGTTTTAGTAGCCACTTTGTCTCAGCCAAGACAGGAGACT
CT

Alignment

ORF - retro_dnov_1426 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 82.82 %
• Parental protein coverage: 73.3 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  SEEESQDRQLKIVVLGDGTSGKTSLATCFAQETFGKQYKQTIGLDFFLRRITLPGNLNVTLQVWDIGGQT
          .EEESQD.QLKIVVLG.GTSG.TSLA.CFA.ETFGK..KQTIGL.F.LRRI.LPG.LNVT.QVWDIGGQT
Retrocopy TEEESQDLQLKIVVLGNGTSGRTSLAMCFAHETFGKHNKQTIGLNFILRRIILPGKLNVT-QVWDIGGQT

Parental  IGGKM-LDKYIYGAQGILLVYDITNYQSFENLEDWYTVVKKVSEESETQPLVALVGNKIDLEHMRTVKPE
          I..K..LDKYIYGAQGILLV.DITNYQS.E.LEDWYT.VKKVSEE..T.PL.ALVGNKIDLEH...VKPE
Retrocopy IRDKI<LDKYIYGAQGILLVCDITNYQSSETLEDWYTIVKKVSEEFKTHPLLALVGNKIDLEHRCRVKPE

Parental  KHLRFCQENGFSSHFVSAKTGDS
          KHLRFCQENGFSSHFVSAKTGDS
Retrocopy KHLRFCQENGFSSHFVSAKTGDS

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012922_ascending_colon	0.00 RPM	20.42 RPM
SRP012922_cerebellum	0.14 RPM	15.12 RPM
SRP012922_heart	0.00 RPM	28.31 RPM
SRP012922_kidney	0.00 RPM	21.63 RPM
SRP012922_liver	0.00 RPM	13.78 RPM
SRP012922_lung	0.15 RPM	25.50 RPM
SRP012922_quadricep_muscle	0.00 RPM	39.64 RPM
SRP012922_spleen	0.00 RPM	25.41 RPM

ChIP-Seq

Dasypus novemcinctus was not studied using ChIP-Seq data.

EST

Dasypus novemcinctus was not studied using EST data.

TSS

Dasypus novemcinctus was not studied using FANTOM5 data.

Lab

retro_dnov_1426 was not experimentally validated.

Homology

Retrocopy orthology:

Dasypus novemcinctus does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 16 parental genes, and 32 retrocopies.

Species	Parental gene accession	Retrocopies number
Choloepus hoffmanni	ENSCHOG00000001523	1 retrocopy	retro_chof_1365,
Callithrix jacchus	ENSCJAG00000012136	2 retrocopies	retro_cjac_2713, retro_cjac_4126,
Dasypus novemcinctus	ENSDNOG00000007750	4 retrocopies	retro_dnov_1426 , retro_dnov_1771, retro_dnov_2419, retro_dnov_381,
Dipodomys ordii	ENSDORG00000012378	1 retrocopy	retro_dord_682,
Homo sapiens	ENSG00000157869	7 retrocopies	retro_hsap_4166, retro_hsap_4259, retro_hsap_4261, retro_hsap_4263, retro_hsap_4265, retro_hsap_4317, retro_hsap_4762,
Gorilla gorilla	ENSGGOG00000024190	1 retrocopy	retro_ggor_2966,
Macropus eugenii	ENSMEUG00000006792	4 retrocopies	retro_meug_1079, retro_meug_1182, retro_meug_1510, retro_meug_913,
Macaca mulatta	ENSMMUG00000022025	1 retrocopy	retro_mmul_1172,
Nomascus leucogenys	ENSNLEG00000016243	1 retrocopy	retro_nleu_2292,
Pongo abelii	ENSPPYG00000014610	1 retrocopy	retro_pabe_3707,
Pan troglodytes	ENSPTRG00000015920	2 retrocopies	retro_ptro_2893, retro_ptro_3171,
Rattus norvegicus	ENSRNOG00000017074	1 retrocopy	retro_rnor_1075,
Sarcophilus harrisii	ENSSHAG00000009274	1 retrocopy	retro_shar_602,
Sus scrofa	ENSSSCG00000022358	2 retrocopies	retro_sscr_482, retro_sscr_514,
Ictidomys tridecemlineatus	ENSSTOG00000004135	1 retrocopy	retro_itri_27,
Tarsius syrichta	ENSTSYG00000010939	2 retrocopies	retro_tsyr_1450, retro_tsyr_1750,