RetrogeneDB ID: retro_cfam_881

Retrocopy location

• Organism: Dog (Canis familiaris)
• Coordinates: 2:23304..24990(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: SLC29A1
• Ensembl ID: ENSCAFG00000001981
• Aliases: SLC29A1, ENT1
• Description: Canis lupus familiaris solute carrier family 29 (nucleoside transporters), member 1 (SLC29A1), nuclear gene encoding mitochondrial protein, mRNA. [Source:RefSeq mRNA;Acc:NM_001003367]

Sequence

>retro_cfam_881
GGCTCTTTCACTGTGTGTGCAGACCATGGTGAGCCCATTGCCCAGGGTACCAAAGTGATCCTCCATCTTAAAGAAGACCA
AAAAGAATATTTAGAGGAGAGGTGTGTCAAAGAAGTGGTGAAGAATCACTCACAGTTCATAGGTTATCCCATCACCCTTT
ATTTGGAGAAGGAATGAGAGAAAGAAATTAGTGATGATGAGGCAGAAGAGGAGAAAGGGGAGAAGGAGGAAGAAGATAAA
GATGATGAGGAGAAACCCAAGATTGAAGATGTGGGTTCAGATGAGGAGGATGATAGTGGGAATGACAAGAGAAAGAAGAC
AAAGAAGATTGAGGAAAAATACACTGATCAAGAATAACTGAACAAGACCAAGCCCATTTGGACCAGAAATCCTGAAGACA
CCACCCAGGAGGAATATGGAGAGTTTTACAAGAGCCTTACTAATGACTGGGAAGATCACCTGGCGGTCAAGCACTTCTCT
GTAGAAGGTCAGTTGGAATTCAGGGCGTTGCTCTTCATCCCTTGTTGGGATCCTTTTGACCTCTTTGAGAACAAGAAGGA
AAAGAACAACTTCAAACTCTATGTCCACCATGGGTTCATCATGGACAGCTGTGATGAATTGATATCAGAGTATCTCAACT
TCATCCGTGGTGTGGTTGACTCCGAGGACCTGCCCCTGAACATCTCCTGAGAAATGCTCCAGCAGAGCAAAATCTTGAAG
GTCATTTGCAAAAATATTGTGAAGAAATGCCTTGAGCTCTTCTCTGAATTGGCAGAAGACAAGGAGAACTATAAGAAATT
CTATGAGGCATTCTCTGAAAACCTAAAGCTTGGAATCCATGAAGATTCCACTAACCGGTGCCGCCTTTCTGAGCTGCTGT
GTTACCACACTTTCCAGTCTGGAGATGAGATTACTTCTCTTTCAGAGTATGTGTCCCATATGAAGGAGACTCAGAAGTCC
ATCTATTACATCACTGGTGAGAGCAAAGAGCAGGTTGCCAACTCCGCTTTTGTGGAGCGAGTGCAGAAACGAGGCTTCGA
GGTACTGTATATGATAGAGCCTATCGACGAGTACTGTGTGCAGCAGCTCAAGGAGTTTGATGGGAAGAGTATTGTCTCAG
TTACCAAGGAGGGCCTGGAGTTGCCTGAGGATGAAGAGGGGAAGAAGAAAATGGAGGAGAGTAAGGCCAAGTTTGAGAAT
CTCTGCAAACTCTGAAGGAAATCTTGGATAAGAAGGTTGAAAAGGTGACCATCTCCAACACGCTGGTGGCTTCACCCTGC
TGTATTGTGACTAGCACCCTATGGCTGGACTGCCAACATGGAGCGGATCATGAAAGCCCAGGCACTCCAGGACAACTCTA
CAATGGGTTATATGATGGCCAAAAAGCACCTGGAGATCAGCCCTGACCACCCCATTGTGGAGATGCTGTGGCAGAAGGCA
GAGGCAGACAAGAATGATAAGGCTGTCAAGGACCTGGTGGTGCTGCTGTTTGAAACAGTTCTGCTCTCCTCCAGCTTCTC
ACTGGAGGATCCCCAGACTCACTCCAACCGTATTTACCATATGATCAAGCTAGGCCTGGGCATTGATGAAGATGAAGTGA
CAGCAGAGGAACCCAGTGCTGCTGTTCCTGATGAGATCCCTCTGCTTGAGGGTGATGAGGATGCCTCTCACATGGAAGAA
GTCAAT

Alignment

ORF - retro_cfam_881 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 90.78 %
• Parental protein coverage: 77.62 %
• Number of stop codons detected: 3
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  GSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEE
          GSFTV.ADHGEPI..GTKVILHLKEDQ.EYLEER.VKEVVK.HSQFIGYPITLYLEKE.EKEISDDEAEE
Retrocopy GSFTVCADHGEPIAQGTKVILHLKEDQKEYLEERCVKEVVKNHSQFIGYPITLYLEKE*EKEISDDEAEE

Parental  EKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYG
          EKGEKEEEDKDDEEKPKIEDVGSDEEDDSG.DK.KKTKKI.EKY.DQE.LNKTKPIWTRNP.D.TQEEYG
Retrocopy EKGEKEEEDKDDEEKPKIEDVGSDEEDDSGNDKRKKTKKIEEKYTDQE*LNKTKPIWTRNPEDTTQEEYG

Parental  EFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPE
          EFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIP...PFDLFENKK.KNN.KLYV...FIMDSCDELI.E
Retrocopy EFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPCWDPFDLFENKKEKNNFKLYVHHGFIMDSCDELISE

Parental  YLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIH
          YLNFIRGVVDSEDLPLNIS.EMLQQSKILKVI.KNIVKKCLELFSELAEDKENYKKFYEAFS.NLKLGIH
Retrocopy YLNFIRGVVDSEDLPLNIS*EMLQQSKILKVICKNIVKKCLELFSELAEDKENYKKFYEAFSENLKLGIH

Parental  EDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVY
          EDSTNR.RLSELL.YHT.QSGDE.TSLSEYVS.MKETQKSIYYITGESKEQVANSAFVERV.KRGFEV.Y
Retrocopy EDSTNRCRLSELLCYHTFQSGDEITSLSEYVSHMKETQKSIYYITGESKEQVANSAFVERVQKRGFEVLY

Parental  MTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKL-MKEILDKKVEKVTIS
          M.EPIDEYCVQQLKEFDGKS.VSVTKEGLELPEDEE.KKKMEESKAKFENLCKL..KEILDKKVEKVTIS
Retrocopy MIEPIDEYCVQQLKEFDGKSIVSVTKEGLELPEDEEGKKKMEESKAKFENLCKL<LKEILDKKVEKVTIS

Parental  NRLVSSPCCIVTST-YGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKA
          N.LV.SPCCIVTST.YGWTANMERIMKAQAL.DNSTMGYMMAKKHLEI.PDHPIVE.L.QKAEADKNDKA
Retrocopy NTLVASPCCIVTST>YGWTANMERIMKAQALQDNSTMGYMMAKKHLEISPDHPIVEMLWQKAEADKNDKA

Parental  VKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPSAAVPDEIPPLEGDEDASRM
          VKDLVVLLFET.LLSS.FSLEDPQTHSNRIY.MIKLGLGIDEDEV.AEEPSAAVPDEIP.LEGDEDAS.M
Retrocopy VKDLVVLLFETVLLSSSFSLEDPQTHSNRIYHMIKLGLGIDEDEVTAEEPSAAVPDEIPLLEGDEDASHM

Parental  EEVD
          EEV.
Retrocopy EEVN

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012049_cerebellum	0.00 RPM	792.27 RPM
SRP017611_brain	0.16 RPM	591.71 RPM
SRP017611_kidney	0.13 RPM	601.80 RPM
SRP017611_liver	0.00 RPM	460.46 RPM

ChIP-Seq

Canis familiaris was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_cfam_881 retrocopy.

TSS

Canis familiaris was not studied using FANTOM5 data.

Lab

retro_cfam_881 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_cfam_881 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 12 parental genes, and 26 retrocopies.

Species	Parental gene accession	Retrocopies number
Canis familiaris	ENSCAFG00000001981	4 retrocopies	retro_cfam_1108, retro_cfam_411, retro_cfam_562, retro_cfam_881 ,
Canis familiaris	ENSCAFG00000018036	1 retrocopy	retro_cfam_1094,
Felis catus	ENSFCAG00000009764	1 retrocopy	retro_fcat_1177,
Gorilla gorilla	ENSGGOG00000008889	2 retrocopies	retro_ggor_1011, retro_ggor_701,
Macropus eugenii	ENSMEUG00000013735	1 retrocopy	retro_meug_1896,
Myotis lucifugus	ENSMLUG00000005239	2 retrocopies	retro_mluc_1604, retro_mluc_865,
Macaca mulatta	ENSMMUG00000022686	2 retrocopies	retro_mmul_1319, retro_mmul_1869,
Mus musculus	ENSMUSG00000023944	3 retrocopies	retro_mmus_1916, retro_mmus_3528, retro_mmus_856,
Otolemur garnettii	ENSOGAG00000011961	1 retrocopy	retro_ogar_2574,
Pongo abelii	ENSPPYG00000016655	2 retrocopies	retro_pabe_2425, retro_pabe_415,
Rattus norvegicus	ENSRNOG00000019834	6 retrocopies	retro_rnor_1561, retro_rnor_1562, retro_rnor_1787, retro_rnor_597, retro_rnor_598, retro_rnor_87,
Tupaia belangeri	ENSTBEG00000014873	1 retrocopy	retro_tbel_1428,