RetrogeneDB ID: retro_mmus_1557

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 17:22016295..22016740(+)
• Located in intron of: ENSMUSG00000053347

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Fam60a
• Ensembl ID: ENSMUSG00000039985
• Aliases: Fam60a, Ppcs1, Pptcs1, Tera
• Description: family with sequence similarity 60, member A [Source:MGI Symbol;Acc:MGI:1929091]

Sequence

>retro_mmus_1557
TGGAATCACGTGGTAAATGCAAGAGCAGGCCCTAGTCTAAAGGCAACATTGAAACCAAAGGAAGTGAAAACTCTATCTGG
AAACAGGATGAAAAGCAACCAGATCAGCAAACTGCAGAAGGAATTTAAACGCCACAACTCTGATGCTCACAGCACCACCT
CAAGTGCCTCTCCAGGCCAGTCCCCATGCTACAGTAACCAGTCAGATGAGGGCTCAGATACAGAGATGGCTTCCAGCTCT
AATAGAACTCCGTTTTTTTTCCTTCTTAAATCTTACCTACTGGAAAAGACAGAAAATATGTTGTGGGATCATCTATAAGG
GCCGTATTGGGGAAGTCCTCATTGACACTCATCTCTTCAAGCCTTGCTGCAGCAGTAAGAAAGCAGCTGCCGAGAAACCC
TAGAAGCAGGAGCCGGCACCTCTGCCCATCTCCACTTAGGAGTGG

Alignment

ORF - retro_mmus_1557 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 90.6 %
• Parental protein coverage: 66.97 %
• Number of stop codons detected: 2
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  WNHVVDARAGPSLKTTLKPKKVKTLSGNRMKSNQISKLQKEFKRHNSDAHSTTSSASPAQSPCYSNQSDE
          WNHVV.ARAGPSLK.TLKPK.VKTLSGNRMKSNQISKLQKEFKRHNSDAHSTTSSASP.QSPCYSNQSDE
Retrocopy WNHVVNARAGPSLKATLKPKEVKTLSGNRMKSNQISKLQKEFKRHNSDAHSTTSSASPGQSPCYSNQSDE

Parental  GSDTEMASSSNRTP-VFSFLDLTYWKRQKICCGIIYKGRFGEVLIDTHLFKPCCSSKKAAAEKPEEQGPA
          GSDTEMASSSNRTP....FL.LTYWKRQKICCGIIYKGR.GEVLIDTHLFKPCCSSKKAAAEKP..Q.PA
Retrocopy GSDTEMASSSNRTP>XXXFLNLTYWKRQKICCGIIYKGRIGEVLIDTHLFKPCCSSKKAAAEKP*KQEPA

Parental  PLPISTQEW
          PLPIST.EW
Retrocopy PLPIST*EW

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.44 RPM	0.12 RPM
SRP007412_cerebellum	0.26 RPM	0.30 RPM
SRP007412_heart	0.16 RPM	0.28 RPM
SRP007412_kidney	0.19 RPM	1.06 RPM
SRP007412_liver	0.05 RPM	0.22 RPM
SRP007412_testis	0.50 RPM	0.73 RPM

ChIP-Seq

RNA Polymerase II activity near the 5' end of retro_mmus_1557 was not detected

EST

No EST(s) were mapped for retro_mmus_1557 retrocopy.

TSS

No TSS is located nearby retro_mmus_1557 retrocopy 5' end.

Lab

retro_mmus_1557 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_1557 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 11 parental genes, and 41 retrocopies.

Species	Parental gene accession	Retrocopies number
Canis familiaris	ENSCAFG00000010685	4 retrocopies	retro_cfam_1120, retro_cfam_1552, retro_cfam_1692, retro_cfam_1837,
Choloepus hoffmanni	ENSCHOG00000007193	17 retrocopies	retro_chof_109, retro_chof_1108, retro_chof_1136, retro_chof_1255, retro_chof_1614, retro_chof_1689, retro_chof_1770, retro_chof_1971, retro_chof_2126, retro_chof_2493, retro_chof_2576, retro_chof_2587, retro_chof_453, retro_chof_511, retro_chof_856, retro_chof_979, retro_chof_998,
Callithrix jacchus	ENSCJAG00000001215	3 retrocopies	retro_cjac_1072, retro_cjac_3154, retro_cjac_3289,
Felis catus	ENSFCAG00000008955	4 retrocopies	retro_fcat_1311, retro_fcat_1339, retro_fcat_58, retro_fcat_965,
Mus musculus	ENSMUSG00000039985	4 retrocopies	retro_mmus_1234, retro_mmus_1471, retro_mmus_1557 , retro_mmus_3331,
Oryctolagus cuniculus	ENSOCUG00000008618	1 retrocopy	retro_ocun_1176,
Rattus norvegicus	ENSRNOG00000046048	1 retrocopy	retro_rnor_2858,
Rattus norvegicus	ENSRNOG00000049943	2 retrocopies	retro_rnor_1121, retro_rnor_2917,
Sarcophilus harrisii	ENSSHAG00000006261	1 retrocopy	retro_shar_670,
Ictidomys tridecemlineatus	ENSSTOG00000007097	1 retrocopy	retro_itri_16,
Tursiops truncatus	ENSTTRG00000008465	3 retrocopies	retro_ttru_1162, retro_ttru_1498, retro_ttru_984,