RetrogeneDB ID: retro_mmul_2172

Retrocopy location

• Organism: Rhesus macaque (Macaca mulatta)
• Coordinates: 7:110079473..110079967(+)
• Located in intron of: ENSMMUG00000015110

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: MMU.9948
• Ensembl ID: ENSMMUG00000018172
• Aliases: None
• Description: replication protein A 32 kDa subunit [Source:RefSeq peptide;Acc:NP_001247563]

Sequence

>retro_mmul_2172
ATGTGGAATAGTGCATTTGAAATCCATGGCTACTCCAGCTATGGAAGAGCCAGTGGTTGTGCAGAGTTCCCTGGGGATTT
GGAACACTGATGTCGTCTCAGAAAAAAATATCAAGAGCCTAAGCCCAGAACATTGTACTCTGTACTGTATCTCAACTGCT
TTTTGCTATTCTTGTTGGTGAAATATTCAAAATTTGGAATGTTGAGATTTCTCAGATCATTACTGTGGAGATAATGAGAT
ATTGCAGAGAGGACTCCAGTCAATATTGTTGATGAAATGGATGACACAACGGTTGCATCAATGGGTGCTCACCAGTGATT
TGATGCAGAGGGTGTCAGTGGTAACCACACTGTGGTTCCTTCAAAAACATATGGGAAAGTGACGGCCATCTGAGACCTTT
TCAGAACAAAAAGAGCCTAGTAGCTTTTAACATCATGCCCCTCAAGGATATGAATGAGTTTTCCACACATATTCTGGAAG
TAGCCAACGGATAT

Alignment

ORF - retro_mmul_2172 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 59.76 %
• Parental protein coverage: 61.48 %
• Number of stop codons detected: 2
• Number of frameshifts detected: 3

Retrocopy - Parental Gene Alignment:

Parental  MWNSGFESYGSSSYGGAGGYTQSPG-GFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNV
          MWNS.FE..G.SSYG.A.G....PG.GFG....SQ..K.SRA.AQ.IV.CT.SQLL.A.LV.E.F.I.NV
Retrocopy MWNSAFEIHGYSSYGRASGCAEFPG<GFGTLMSSQ-KKISRA*AQNIVLCTVSQLLFAILVGEIFKIWNV

Parental  EISQVTIVGIIRH-AEKAPTNIVYKIDDMTAAPMDVRQWVDTDDASSENTVVPPETYVKVA-GHLRSFQN
          EISQ...V.I.R..AE..P.NIV...DD.T.A.M...Q..D....S...TVVP..TY.KV..GHLR.FQN
Retrocopy EISQIITVEIMRY>AERTPVNIVDEMDDTTVASMGAHQ*FDAEGVSGNHTVVPSKTYGKVT<GHLRPFQN

Parental  KKSLVAFKIMPLEDMNEFTTHILEVINAH
          KKSLVAF.IMPL.DMNEF.THILEV.N..
Retrocopy KKSLVAFNIMPLKDMNEFSTHILEVANGY

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	11.21 RPM
SRP007412_brain_prefrontal_cortex	0.00 RPM	8.25 RPM
SRP007412_cerebellum	0.00 RPM	15.76 RPM
SRP007412_heart	0.00 RPM	9.95 RPM
SRP007412_kidney	0.00 RPM	15.02 RPM
SRP007412_liver	0.00 RPM	7.76 RPM
SRP007412_testis	0.00 RPM	41.16 RPM

ChIP-Seq

Macaca mulatta was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_mmul_2172 retrocopy.

TSS

Macaca mulatta was not studied using FANTOM5 data.

Lab

retro_mmul_2172 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmul_2172 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 14 parental genes, and 26 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000006225	5 retrocopies	retro_btau_1093, retro_btau_1228, retro_btau_1359, retro_btau_406, retro_btau_633,
Callithrix jacchus	ENSCJAG00000009473	1 retrocopy	retro_cjac_225,
Homo sapiens	ENSG00000117748	2 retrocopies	retro_hsap_704, retro_hsap_815,
Gorilla gorilla	ENSGGOG00000015287	2 retrocopies	retro_ggor_599, retro_ggor_668,
Loxodonta africana	ENSLAFG00000014387	1 retrocopy	retro_lafr_519,
Macaca mulatta	ENSMMUG00000018172	2 retrocopies	retro_mmul_1081, retro_mmul_2172 ,
Nomascus leucogenys	ENSNLEG00000009702	3 retrocopies	retro_nleu_1409, retro_nleu_250, retro_nleu_2511,
Oryctolagus cuniculus	ENSOCUG00000016194	1 retrocopy	retro_ocun_1180,
Otolemur garnettii	ENSOGAG00000015583	2 retrocopies	retro_ogar_1838, retro_ogar_2066,
Pongo abelii	ENSPPYG00000001651	2 retrocopies	retro_pabe_523, retro_pabe_728,
Pan troglodytes	ENSPTRG00000000420	1 retrocopy	retro_ptro_566,
Pteropus vampyrus	ENSPVAG00000001449	1 retrocopy	retro_pvam_166,
Ictidomys tridecemlineatus	ENSSTOG00000004433	2 retrocopies	retro_itri_1257, retro_itri_773,
Tursiops truncatus	ENSTTRG00000007507	1 retrocopy	retro_ttru_873,