RetrogeneDB ID: retro_ggor_2429

Retrocopy location

• Organism: Gorilla (Gorilla gorilla)
• Coordinates: 6:61966571..61967129(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: DHFR
• Ensembl ID: ENSGGOG00000022366
• Aliases: None
• Description: None

Sequence

>retro_ggor_2429
ATGGTTCATCCGCTAAACCGCATTGTCACTGTTTCCCAGAAGATGGGCATCATCAGGAATGGGGACCTGCCCTGACCCCA
GCTCAAAAATAAATTCGATTCCAAAGAATGACCACACCCTCTTCAGCAGAGGGTAAACAACATTTAGTATTTTTGATTAG
GAAGAACTGGTTCTCGATTACTGAGAAGAATCAACCTTTAAAGTATATAATTAATTTAGTTGTCAGTAGAGAATCCAAGG
AACCACTGCAAAGACCTTTTCTTGACTAAAGTCTGGGTGATGCCTTAAAACGTATTGAGCAACTAAAATTAGCAAATAAA
CAAGACGTGTTTTTTACAGTGGGAGGCAGTTCTGTTTATAAGGAATCCATGAATTAAGAGACCATTTTAAACTATTTGTG
ACATGGATCATGCAGGACTTTCAAAGTGACACTTTTTTCCCCTAGAAGATGATTTAGAGAAATATAAACTTTTCCCAGAA
TATCCACAAGGTGTTGTCTCTGATGTGGAGGAGGAGAAAGGCATTAAGTACAAATTTGAAGTATATGAAAAGAATGAT

Alignment

ORF - retro_ggor_2429 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 62.11 %
• Parental protein coverage: 100.0 %
• Number of stop codons detected: 5
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  MVGSLNCIVAVSQNMGIGKNGVLPWP-PLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLK
          MV..LN.IV.VSQ.MGI..NG.LP.P.P.....R.FQRMTT.SS.EGKQ.LV....K.WFSI.EKN.PLK
Retrocopy MVHPLNRIVTVSQKMGIIRNGDLP*P<PAQK*IR-FQRMTTPSSAEGKQHLVFLIRKNWFSITEKNQPLK

Parental  GRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRI
          ..INLV.SRE.KEP.Q...FL..SL.DALK..EQ..LANK.D....VGGSSVYKE.MN....L.......
Retrocopy YIINLVVSRESKEPLQ-RPFLD*SLGDALKRIEQLKLANKQDVFFTVGGSSVYKESMN*ETILNYL*HGS

Parental  MQDFESDTFFP-EIDLEKYKLLPEYP-GVLSDVQEEKGIKYKFEVYEKND
          ...F....F.P.E.DLEKYKL.PEYP.GV.SDV.EEKGIKYKFEVYEKND
Retrocopy CRTFKVTLFSP>EDDLEKYKLFPEYPQGVVSDVEEEKGIKYKFEVYEKND

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain_prefrontal_cortex	0.00 RPM	1.82 RPM
SRP007412_cerebellum	0.00 RPM	2.32 RPM
SRP007412_heart	0.00 RPM	0.59 RPM
SRP007412_kidney	0.00 RPM	7.28 RPM
SRP007412_liver	0.00 RPM	5.59 RPM
SRP007412_testis	0.00 RPM	5.91 RPM

ChIP-Seq

Gorilla gorilla was not studied using ChIP-Seq data.

EST

Gorilla gorilla was not studied using EST data.

TSS

Gorilla gorilla was not studied using FANTOM5 data.

Lab

retro_ggor_2429 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ggor_2429 has 3 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_3602
Pan troglodytes	retro_ptro_2988
Callithrix jacchus	retro_cjac_2446

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Parental genes homology:

Parental genes homology involve 19 parental genes, and 52 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000015750	2 retrocopies	retro_amel_218, retro_amel_842,
Bos taurus	ENSBTAG00000007681	1 retrocopy	retro_btau_351,
Callithrix jacchus	ENSCJAG00000019556	4 retrocopies	retro_cjac_2017, retro_cjac_2298, retro_cjac_2446, retro_cjac_531,
Equus caballus	ENSECAG00000026827	1 retrocopy	retro_ecab_513,
Felis catus	ENSFCAG00000026745	3 retrocopies	retro_fcat_579, retro_fcat_705, retro_fcat_841,
Homo sapiens	ENSG00000228716	4 retrocopies	retro_hsap_1927, retro_hsap_2119, retro_hsap_3602, retro_hsap_6,
Gorilla gorilla	ENSGGOG00000022366	2 retrocopies	retro_ggor_1959, retro_ggor_2429 ,
Myotis lucifugus	ENSMLUG00000012200	2 retrocopies	retro_mluc_1109, retro_mluc_2577,
Macaca mulatta	ENSMMUG00000018283	13 retrocopies	retro_mmul_1496, retro_mmul_425, retro_mmul_692, retro_mmul_693, retro_mmul_695, retro_mmul_700, retro_mmul_701, retro_mmul_704, retro_mmul_738, retro_mmul_760, retro_mmul_768, retro_mmul_82, retro_mmul_994,
Monodelphis domestica	ENSMODG00000024860	3 retrocopies	retro_mdom_1685, retro_mdom_604, retro_mdom_703,
Mustela putorius furo	ENSMPUG00000008704	1 retrocopy	retro_mputfur_1107,
Mus musculus	ENSMUSG00000021707	1 retrocopy	retro_mmus_3326,
Nomascus leucogenys	ENSNLEG00000012989	3 retrocopies	retro_nleu_1193, retro_nleu_2314, retro_nleu_2631,
Oryctolagus cuniculus	ENSOCUG00000005127	3 retrocopies	retro_ocun_1351, retro_ocun_1640, retro_ocun_650,
Otolemur garnettii	ENSOGAG00000025320	1 retrocopy	retro_ogar_222,
Pongo abelii	ENSPPYG00000015600	2 retrocopies	retro_pabe_2959, retro_pabe_41,
Pan troglodytes	ENSPTRG00000017042	3 retrocopies	retro_ptro_109, retro_ptro_1551, retro_ptro_2988,
Rattus norvegicus	ENSRNOG00000013521	1 retrocopy	retro_rnor_2030,
Sus scrofa	ENSSSCG00000014125	2 retrocopies	retro_sscr_104, retro_sscr_1131,