RetrogeneDB ID: retro_eeur_610

Retrocopy location

• Organism: Hedgehog (Erinaceus europaeus)
• Coordinates: scaffold_362840:10537..11453(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: EIF4A1
• Ensembl ID: ENSEEUG00000003581
• Aliases: None
• Description: eukaryotic translation initiation factor 4A1 [Source:HGNC Symbol;Acc:3282]

Sequence

>retro_eeur_610
ATGTCTGTAAGTCAGGATTCCTGATCCAGAGACAATGGCCCCGATAGGATGGAGCCCGAAGGTGTCATCGAGAGTAACTG
GAATGAGATTGTGGACAGCTTTGATGACATGAATCTCTCTGAGTCCCTTCTCCGTTGCATATATCCCTATGCCTTTGAGA
ACCCATCTGCCACCCAGCAGCGAGCCATTCTTAAGGGTTATGGTATGACCACTCAAGGCCAATCTGGGACTGGGAAAACA
GCTACTTTTGCCATATCGATTCTGTAGCAGATAGAATTGGATTTAAAGGCCACCCAGGCCTTGGTGCTAGCACCCACAAG
AGAATTGGTTCAACAGATACAAAAGGTGGTCATGGCATTAGGAGATTACATGGGTGCTTCCTGCCATGCCTTTATTGGTG
GTACCAATGTCCGTGCAGAAGTGCAGAAGCTGCAGATGGGAGCTTCCCATATAATTATTGGTACTCCAGGCCATGTATTT
GATAGCCGTAGATACTCGTCTCCCAAATATATCAAGACGTTCGTGTTGGATGAAGCTGATGAAATGTTAAGCCGAGGATT
CAAGGACCAGATTTATGACATATTCCAAAAGCTCAATAGCAATACCCAGGTGGTTTTGCTGTCAGCTACCATGCCTTCTG
ATGTGCTTGAGGTGACCAAGAAGTTTGTGAAGGACCCCATTTGGATTATTGTCAAGAAAGAAGATCTCACTCTGGAGGGC
ACCACCAATTCTACATCAATGTGAAACAAGAGGAGTGGAAACTTGACACACTCTGTGACTTGTATGAAACCTTGACCACC
ACACAAGCCGTCATCTTTATCAATACCCTAAGGAAGGTTGATTGGCTTACTGAGAAGATGCATGCCCCAAGACTTCACTG
TCTCTGCCATGCGGTAGGGACATGGACCAAAAGGAG

Alignment

ORF - retro_eeur_610 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 85.62 %
• Parental protein coverage: 76.35 %
• Number of stop codons detected: 2
• Number of frameshifts detected: 3

Retrocopy - Parental Gene Alignment:

Parental  MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGY
          MS.SQDS.SRDNGPD.MEPEGVIESNWNEIVDSFDDMNLSESLLR.IY.Y.FE.PSA.QQRAIL...KGY
Retrocopy MSVSQDS*SRDNGPDRMEPEGVIESNWNEIVDSFDDMNLSESLLRCIYPYAFENPSATQQRAIL---KGY

Parental  DVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNV
          ....Q.QSGTGKTATFAISIL.QIELDLKATQALVLAPTREL.QQIQKVVMALGDYMGASCHA.IGGTNV
Retrocopy GMTTQGQSGTGKTATFAISIL*QIELDLKATQALVLAPTRELVQQIQKVVMALGDYMGASCHAFIGGTNV

Parental  RAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVV
          RAEVQKLQM.A.HII.GTPG.VFD...RRY.SPKYIK.FVLDEADEMLSRGFKDQIYDIFQKLNSNTQVV
Retrocopy RAEVQKLQMGASHIIIGTPGHVFD--SRRYSSPKYIKTFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVV

Parental  LLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGI-RQFYINVEREEWKLDTLCDLYETLTITQAVIFI
          LLSATMPSDVLEVTKKF..DPI.I.VKKE.LTLEG...QFYINV..EEWKLDTLCDLYETLT.TQAVIFI
Retrocopy LLSATMPSDVLEVTKKFVKDPIWIIVKKEDLTLEGT<HQFYINVKQEEWKLDTLCDLYETLTTTQAVIFI

Parental  NTRRKVDWLTEKMHA-RDFTVSAMH-GDMDQKE
          NT.RKVDWLTEKMHA..DFTVSAM...DMDQKE
Retrocopy NTLRKVDWLTEKMHA>QDFTVSAMR>RDMDQKE

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP017611_brain	0.00 RPM	204.49 RPM
SRP017611_kidney	0.19 RPM	331.57 RPM
SRP017611_liver	0.16 RPM	204.19 RPM

ChIP-Seq

Erinaceus europaeus was not studied using ChIP-Seq data.

EST

Erinaceus europaeus was not studied using EST data.

TSS

Erinaceus europaeus was not studied using FANTOM5 data.

Lab

retro_eeur_610 was not experimentally validated.

Homology

Retrocopy orthology:

Erinaceus europaeus does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 15 parental genes, and 65 retrocopies.

Species	Parental gene accession	Retrocopies number
Cavia porcellus	ENSCPOG00000004900	1 retrocopy	retro_cpor_1088,
Erinaceus europaeus	ENSEEUG00000003581	1 retrocopy	retro_eeur_610 ,
Felis catus	ENSFCAG00000024897	1 retrocopy	retro_fcat_370,
Homo sapiens	ENSG00000161960	10 retrocopies	retro_hsap_1004, retro_hsap_1182, retro_hsap_1252, retro_hsap_1270, retro_hsap_1796, retro_hsap_2520, retro_hsap_4836, retro_hsap_4873, retro_hsap_4931, retro_hsap_610,
Gorilla gorilla	ENSGGOG00000024702	8 retrocopies	retro_ggor_1321, retro_ggor_1572, retro_ggor_3015, retro_ggor_537, retro_ggor_798, retro_ggor_920, retro_ggor_983, retro_ggor_998,
Macropus eugenii	ENSMEUG00000010780	2 retrocopies	retro_meug_158, retro_meug_179,
Myotis lucifugus	ENSMLUG00000003415	3 retrocopies	retro_mluc_1047, retro_mluc_1229, retro_mluc_1805,
Macaca mulatta	ENSMMUG00000013499	1 retrocopy	retro_mmul_1280,
Monodelphis domestica	ENSMODG00000007090	3 retrocopies	retro_mdom_1282, retro_mdom_326, retro_mdom_711,
Mus musculus	ENSMUSG00000059796	7 retrocopies	retro_mmus_1810, retro_mmus_1853, retro_mmus_2320, retro_mmus_2473, retro_mmus_3122, retro_mmus_325, retro_mmus_527,
Nomascus leucogenys	ENSNLEG00000009547	7 retrocopies	retro_nleu_2109, retro_nleu_2117, retro_nleu_230, retro_nleu_2470, retro_nleu_2769, retro_nleu_440, retro_nleu_701,
Pan troglodytes	ENSPTRG00000008694	10 retrocopies	retro_ptro_1201, retro_ptro_1465, retro_ptro_2954, retro_ptro_3112, retro_ptro_3247, retro_ptro_450, retro_ptro_750, retro_ptro_810, retro_ptro_862, retro_ptro_870,
Rattus norvegicus	ENSRNOG00000030628	3 retrocopies	retro_rnor_1059, retro_rnor_2213, retro_rnor_2306,
Sorex araneus	ENSSARG00000006017	7 retrocopies	retro_sara_224, retro_sara_383, retro_sara_461, retro_sara_567, retro_sara_6, retro_sara_605, retro_sara_89,
Ictidomys tridecemlineatus	ENSSTOG00000024814	1 retrocopy	retro_itri_610,