RetrogeneDB ID: retro_cfam_2188

Retrocopy location

• Organism: Dog (Canis familiaris)
• Coordinates: X:83364938..83365359(-)
• Located in intron of: ENSCAFG00000018083

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: POLR2D
• Ensembl ID: ENSCAFG00000004311
• Aliases: None
• Description: polymerase (RNA) II (DNA directed) polypeptide D [Source:HGNC Symbol;Acc:9191]

Sequence

>retro_cfam_2188
GTGGCCGCTGGAGGTAGCGATCCGCGGGCGGGCGACGTCGAGGAGGATCCCTCACAGCTTATCTTTCCCAAAGAGTTTGA
AACAGCGGAGACATTACTAAATTCAGTTCATATGCTTCTGGAGCATCGAAAGCAACAGAATGAGAGTGCAGAAGATGAAC
AGAAACGTTCTGAGGTCTTCATGAAAACTTTAAATTATACAGCCCGTTTCAGCCGTTTCAAAAACAGAGACCATTGCCAG
TGTCTGTAGCTTGTTGCTCCAGAAGAAACTTCATAAGTTTGAGTTGGCCTGTTTGGCCAGCCTTTGCCCAGAGACTGCTG
AGGAGTCAAAGGCACTGATACCAAGCCTGGAGGGGCGGTTTGAAGATGAGGAGCTACAGCAGATTCTTGATGATATCCAG
ACCAAGCGCAGCTTTCAATAT

Alignment

ORF - retro_cfam_2188 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 90.28 %
• Parental protein coverage: 100.0 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTAR
          .AAGGSDPRAGDVEED.SQLIFPKEFETAETLLNS.VHMLLEHRKQQNESAEDEQ..SEVFMKTLNYTAR
Retrocopy VAAGGSDPRAGDVEEDPSQLIFPKEFETAETLLNS-VHMLLEHRKQQNESAEDEQKRSEVFMKTLNYTAR

Parental  -FSRFKNR-ETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKR
          .F..F....ETIASV.SLLLQKKLHKFELACLA.LCPETAEESKALIPSLEGRFEDEELQQILDDIQTKR
Retrocopy <FQPFQKQ<ETIASVCSLLLQKKLHKFELACLASLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKR

Parental  SFQY
          SFQY
Retrocopy SFQY

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012049_cerebellum	0.00 RPM	18.77 RPM
SRP017611_brain	0.00 RPM	9.05 RPM
SRP017611_kidney	0.07 RPM	15.25 RPM
SRP017611_liver	0.07 RPM	3.26 RPM

ChIP-Seq

Canis familiaris was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_cfam_2188 retrocopy.

TSS

Canis familiaris was not studied using FANTOM5 data.

Lab

retro_cfam_2188 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_cfam_2188 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 17 parental genes, and 26 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000011746	1 retrocopy	retro_amel_1020,
Canis familiaris	ENSCAFG00000004311	2 retrocopies	retro_cfam_1403, retro_cfam_2188 ,
Choloepus hoffmanni	ENSCHOG00000013850	3 retrocopies	retro_chof_1003, retro_chof_1568, retro_chof_1920,
Dasypus novemcinctus	ENSDNOG00000008527	1 retrocopy	retro_dnov_734,
Felis catus	ENSFCAG00000027816	1 retrocopy	retro_fcat_1148,
Gorilla gorilla	ENSGGOG00000004723	1 retrocopy	retro_ggor_1658,
Macaca mulatta	ENSMMUG00000001989	1 retrocopy	retro_mmul_1011,
Monodelphis domestica	ENSMODG00000018653	4 retrocopies	retro_mdom_1320, retro_mdom_457, retro_mdom_689, retro_mdom_851,
Mustela putorius furo	ENSMPUG00000016685	1 retrocopy	retro_mputfur_611,
Mus musculus	ENSMUSG00000024258	1 retrocopy	retro_mmus_1331,
Oryctolagus cuniculus	ENSOCUG00000021449	1 retrocopy	retro_ocun_35,
Ochotona princeps	ENSOPRG00000005327	2 retrocopies	retro_opri_233, retro_opri_725,
Pongo abelii	ENSPPYG00000012759	1 retrocopy	retro_pabe_1933,
Pan troglodytes	ENSPTRG00000012436	1 retrocopy	retro_ptro_1571,
Sorex araneus	ENSSARG00000009037	1 retrocopy	retro_sara_420,
Sarcophilus harrisii	ENSSHAG00000014817	1 retrocopy	retro_shar_728,
Ictidomys tridecemlineatus	ENSSTOG00000023329	3 retrocopies	retro_itri_1323, retro_itri_145, retro_itri_600,