RetrogeneDB ID: retro_mmus_2750

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 6:76121761..76122894(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Paics
• Ensembl ID: ENSMUSG00000029247
• Aliases: Paics, 2610511I09Rik, ADE2H1, AIRC, PAIS
• Description: phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoribosylaminoimidazole, succinocarboxamide synthetase [Source:MGI Symbol;Acc:MGI:1914304]

Sequence

>retro_mmus_2750
CACCTGGAGGGCAAAGCTGCAATCTCCAACAAGATCTCCAAGCTGCATATTTCAGTTGTTACAGGAAGCTGGTATAAAAA
CTGCTTTCACCAAGAAATTTGGGAGACTGCTTGCATTGCACCCCTATGTGAAATGATTCCAATTGAATGGGTATGTGGAA
GACTAGCAACTGGATCTTTTCTCAAAAGGAACCCTGGTGTAAAGGAGGTATAAGTTTTATCTACCCAAACTGGAGACGTT
CTTAGAGGATGATGCCAGAAATGATCCACAGTGGTCTGAGGAGCAACTCATCGCTGCAAAATTGCATTTTGCTGGCCTTG
TTATAGGGCAGACTGAAGTGGACATCATGAGTCACACCACACAAGTTATTTTGAAAATCTGGAGAAATCCTGGCTGCCGC
AGAACTGTACGCTGGTTTATGCGAAGATTGAACTTGGCGTTGATGTAACAACCAAAGAGATTGTTCTTGCTGATGTCATT
GATAATGATTCCTGGAGACTAAGGCCATCGGGAGATCGGAGCCAGCAGAAAGACAAACAGTCTGACCATGATCTCAAAGA
AGTAATTCCGGAAAGACTCCAGATGGTGAAGAAAAACTCTGAGTGGGTTGAAAACTGAGTGGAGTTGCTTCTCAAGTCAG
ACAGTCAGTGCAGGGTGGTAGTGCTGATGGGCTCAACCTCTGACCTTGGTCACTGCGAAAAACTAAGAAGGCTTGTATAA
ATTTTGGAATTCCGTTTGAACTTAGAGTAACATCCATGCATAAAGGACCAGATGAAACTTTGAGGGTTAAAGCAGAATAT
GAAGGACATAACATAGCTATTGTATTTGTGTCAGTGGGGGGCAGAAGCAATGGTCTAGGCCCAGTAATGTCTGGTAATAC
TGCATATCCGGTTATCGGCTGTCCCCCAATCACACCAGACTTGGGTGCTCAGGATGCGTGGTCATCTCTTCAATTGACCA
GTGGTCTCGGCTGTTCAACTATACTTTCTCCAGAAGAATCATTTCAGTTCGCTGCTCAGATATTTGGGTTAAACAATTAT
TTGGTGTGGGCCAAACTTCGAGCTTATATGCTTATATTGAACACATGGATATCCTTATAGCAGGCTGATAAAAAAATCAG
AGAATGCAATTTG

Alignment

ORF - retro_mmus_2750 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 82.34 %
• Parental protein coverage: 88.71 %
• Number of stop codons detected: 2
• Number of frameshifts detected: 6

Retrocopy - Parental Gene Alignment:

Parental  HLEGKAAISNKIT-SCIFQLLQEAGIKTAFTKKCG-ETAFIAPQCEMIPIEWVCRRIATGSFLK-RNPGV
          HLEGKAAISNKI..SCIFQLLQEAGIKTAFTKK.G.ETA.IAP.CEMIPIEWVC.R.ATGSFLK...P..
Retrocopy HLEGKAAISNKIS>SCIFQLLQEAGIKTAFTKKFG<ETACIAPLCEMIPIEWVCGRLATGSFLK<KEPWC

Parental  QEG-YKFYPPKVEMFFKDDANNDPQWSEEQLIAAKFCFAGLVIGQTEVDIMSHATQAI-FEILEKSWLPQ
          ..G.YKFY.PK.E.F..DDA.NDPQWSEEQLIAAK..FAGLVIGQTEVDIMSH.TQ.I.FE.LEKSWLPQ
Retrocopy KGG<YKFYLPKLETFLEDDARNDPQWSEEQLIAAKLHFAGLVIGQTEVDIMSHTTQVI<FENLEKSWLPQ

Parental  DCTLVDMKIEFGVDVTTKEIVLADVIDNDSWRLWPSGDRSQQKDKQSYRDLKEVTPEGLQMVKKNFEWVA
          .CTLV..KIE.GVDVTTKEIVLADVIDNDSWRL.PSGDRSQQKDKQS..DLKEV.PE.LQMVKKN.EWV.
Retrocopy NCTLVYAKIELGVDVTTKEIVLADVIDNDSWRLRPSGDRSQQKDKQSDHDLKEVIPERLQMVKKNSEWVE

Parental  DRVELLLKSDSQCRVVVLMGSTSDLGHCEKI-KKACGNFGIPCELRVTSAHKGPDETLRIKAEYEGDGIP
          ..VELLLKSDSQCRVVVLMGSTSDLGHCEK..KKAC.NFGIP.ELRVTS.HKGPDETLR.KAEYEG..I.
Retrocopy N*VELLLKSDSQCRVVVLMGSTSDLGHCEKL<KKACINFGIPFELRVTSMHKGPDETLRVKAEYEGHNIA

Parental  TVFVSVAGRSNGLGPVLSGNTAYPVISCPPITPDWGAQDVWSSLRLPSGIGCSTILSPEGSAQFAAQIFG
          .VFVSV.GRSNGLGPV.SGNTAYPVI.CPPITPD.GAQD.WSSL.L.SG.GCSTILSPE.S.QFAAQIFG
Retrocopy IVFVSVGGRSNGLGPVMSGNTAYPVIGCPPITPDLGAQDAWSSLQLTSGLGCSTILSPEESFQFAAQIFG

Parental  LNNHLVWAKLRA--SILNTWISLKQADKKVRQCNL
          LNN.LVWAKLRA...ILNTWISL.QADKK.R.CNL
Retrocopy LNNYLVWAKLRAYMLILNTWISL*QADKKIRECNL

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	72.47 RPM
SRP007412_cerebellum	0.00 RPM	76.99 RPM
SRP007412_heart	0.03 RPM	100.89 RPM
SRP007412_kidney	0.00 RPM	159.50 RPM
SRP007412_liver	0.00 RPM	189.96 RPM
SRP007412_testis	0.00 RPM	40.07 RPM

ChIP-Seq

RNA Polymerase II actvity near the 5' end of retro_mmus_2750 was not detected

EST

No EST(s) were mapped for retro_mmus_2750 retrocopy.

TSS

No TSS is located nearby retro_mmus_2750 retrocopy 5' end.

Lab

retro_mmus_2750 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_2750 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 15 parental genes, and 29 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000010577	1 retrocopy	retro_btau_508,
Cavia porcellus	ENSCPOG00000010749	1 retrocopy	retro_cpor_969,
Dasypus novemcinctus	ENSDNOG00000013413	1 retrocopy	retro_dnov_284,
Echinops telfairi	ENSETEG00000015242	2 retrocopies	retro_etel_1918, retro_etel_341,
Homo sapiens	ENSG00000128050	3 retrocopies	retro_hsap_3802, retro_hsap_3915, retro_hsap_3927,
Gorilla gorilla	ENSGGOG00000024392	1 retrocopy	retro_ggor_2643,
Mus musculus	ENSMUSG00000029247	6 retrocopies	retro_mmus_1748, retro_mmus_2750 , retro_mmus_2858, retro_mmus_3126, retro_mmus_653, retro_mmus_971,
Nomascus leucogenys	ENSNLEG00000008786	2 retrocopies	retro_nleu_1274, retro_nleu_2156,
Otolemur garnettii	ENSOGAG00000003575	2 retrocopies	retro_ogar_2138, retro_ogar_865,
Procavia capensis	ENSPCAG00000008531	1 retrocopy	retro_pcap_172,
Pan troglodytes	ENSPTRG00000016081	2 retrocopies	retro_ptro_2662, retro_ptro_2670,
Rattus norvegicus	ENSRNOG00000002101	3 retrocopies	retro_rnor_1891, retro_rnor_2768, retro_rnor_804,
Tupaia belangeri	ENSTBEG00000006751	1 retrocopy	retro_tbel_3697,
Tarsius syrichta	ENSTSYG00000010497	2 retrocopies	retro_tsyr_2170, retro_tsyr_329,
Drosophila melanogaster	FBgn0020513	1 retrocopy	retro_dmel_82,