RetrogeneDB ID: retro_mdom_2029

Retrocopy location

• Organism: Opossum (Monodelphis domestica)
• Coordinates: X:77624442..77624869(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: UBE2L3
• Ensembl ID: ENSMODG00000017665
• Aliases: None
• Description: ubiquitin-conjugating enzyme E2L 3 [Source:HGNC Symbol;Acc:12488]

Sequence

>retro_mdom_2029
AAGGAGCTGGAAGAAATCCACGAGTGTGGGATGAAAAACTTCCATAACATCCAGGTTGGTGAAACTAATTTATTGACTTG
GCAAGGACTCATCATTCCTAACAACCCTCCATATGACAAGGGAGCCTTCAGAATTGAAATCACTGTTCCAATCAGAGTAT
CCATTCAAACCACCCAAGATTATATTTAAAACAAAGATTTACCACCCGAATATGGATGAAAAGGGGCAGGTTTGTCTGCC
AGTAATCAGTGCTGAAAACTGGAAACCAGCAACCAAAACTGGCCAAGTCATCTAGTCCCTCATAGCACTGCTGAATGACC
CCCAGCCTGAGCACCCCGTTTGGGCTGAGCTAGCCGAAGAATACTAAAAATTCTGTAAGAACACTGAAGAGTTTACAAAA
ACATATGGGGAAAAGTGACCCGTGGAC

Alignment

ORF - retro_mdom_2029 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 80.95 %
• Parental protein coverage: 94.81 %
• Number of stop codons detected: 3
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  QELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFP-AEYPFKPPKITFKTKIYHP
          .ELEEI..CGMKNF.NIQV.E.NLLTWQGLI.P.NPPYDKGAFRIEI..P..EYPFKPPKI.FKTKIYHP
Retrocopy KELEEIHECGMKNFHNIQVGETNLLTWQGLIIPNNPPYDKGAFRIEITVP>SEYPFKPPKIIFKTKIYHP

Parental  NIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKY
          N.DEKGQVCLPVISAENWKPATKT.QVI.SLIAL.NDPQPEHP..A.LAEEY.K....FCKN.EEFTK.Y
Retrocopy NMDEKGQVCLPVISAENWKPATKTGQVI*SLIALLNDPQPEHPVWAELAEEY*K----FCKNTEEFTKTY

Parental  GEKRPVD
          GEK.PVD
Retrocopy GEK*PVD

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	0.00 RPM
SRP007412_cerebellum	0.00 RPM	0.00 RPM
SRP007412_heart	0.00 RPM	0.00 RPM
SRP007412_kidney	0.00 RPM	0.00 RPM
SRP007412_liver	0.00 RPM	0.00 RPM
SRP007412_testis	0.00 RPM	0.00 RPM

ChIP-Seq

Monodelphis domestica was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_mdom_2029 retrocopy.

TSS

Monodelphis domestica was not studied using FANTOM5 data.

Lab

retro_mdom_2029 was not experimentally validated.

Homology

Retrocopy orthology:

Monodelphis domestica does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 18 parental genes, and 44 retrocopies.

Species	Parental gene accession	Retrocopies number
Canis familiaris	ENSCAFG00000015284	1 retrocopy	retro_cfam_1090,
Callithrix jacchus	ENSCJAG00000015818	9 retrocopies	retro_cjac_1813, retro_cjac_1815, retro_cjac_1998, retro_cjac_2073, retro_cjac_2511, retro_cjac_2576, retro_cjac_2856, retro_cjac_3174, retro_cjac_3296,
Cavia porcellus	ENSCPOG00000006044	2 retrocopies	retro_cpor_692, retro_cpor_835,
Dipodomys ordii	ENSDORG00000011406	1 retrocopy	retro_dord_357,
Equus caballus	ENSECAG00000019274	1 retrocopy	retro_ecab_116,
Felis catus	ENSFCAG00000008008	1 retrocopy	retro_fcat_369,
Gorilla gorilla	ENSGGOG00000028073	3 retrocopies	retro_ggor_1425, retro_ggor_861, retro_ggor_924,
Myotis lucifugus	ENSMLUG00000001547	1 retrocopy	retro_mluc_32,
Monodelphis domestica	ENSMODG00000010301	2 retrocopies	retro_mdom_1679, retro_mdom_1688,
Monodelphis domestica	ENSMODG00000011783	2 retrocopies	retro_mdom_17, retro_mdom_884,
Monodelphis domestica	ENSMODG00000017665	2 retrocopies	retro_mdom_2029 , retro_mdom_956,
Mus musculus	ENSMUSG00000038965	10 retrocopies	retro_mmus_1619, retro_mmus_1620, retro_mmus_2147, retro_mmus_2212, retro_mmus_2272, retro_mmus_2315, retro_mmus_3205, retro_mmus_3714, retro_mmus_541, retro_mmus_995,
Nomascus leucogenys	ENSNLEG00000000192	1 retrocopy	retro_nleu_97,
Pongo abelii	ENSPPYG00000011602	1 retrocopy	retro_pabe_99,
Pan troglodytes	ENSPTRG00000014114	1 retrocopy	retro_ptro_123,
Rattus norvegicus	ENSRNOG00000001862	4 retrocopies	retro_rnor_1602, retro_rnor_2633, retro_rnor_2799, retro_rnor_526,
Tetraodon nigroviridis	ENSTNIG00000010600	1 retrocopy	retro_tnig_16,
Takifugu rubripes	ENSTRUG00000003392	1 retrocopy	retro_trub_39,