RetrogeneDB ID: retro_ggor_537

Retrocopy location

• Organism: Gorilla (Gorilla gorilla)
• Coordinates: 10:105267876..105268717(+)
• Located in intron of: ENSGGOG00000016441

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: EIF4A1
• Ensembl ID: ENSGGOG00000024702
• Aliases: None
• Description: None

Sequence

>retro_ggor_537
TCCAGAGACAATGGTCCCGATGAGATGGAGCCTGAAGGCATCATTGAGAGAAACTGAAATGAGATTGTTGACAGTTTTGA
TGATATGAACCTCTTGGAATCCCTCCTCCATGGCATCTATGCCTATGGTTTTGAGAAGTCCTCTGCCATCCAGCAGCGAG
CCATTCTTCTTGTATCAAGGAATATGATGTGATCACTCAAGCCCTATCTGGGAATGGGAAAATGGCCACTTTTGCCATAT
CAATTCTGCAGTAGATTGAATTAGATCTAAAGGCTACCCACACCTTGGTCCCAGCATCCACTTGATAATTGGCTCAAAAG
ATAGAAAAGGTGGTCATGACACTAGGAGACTACATGGGTGCCTCCTGTCATGGCTGTATTGGGGGCACCAACGTGCAATG
CTGAGATGCAGAAACTACAGATGGAAGTTCCCCATATCATCGTGGGTACCCCTGGCCATATGTCTGATATGCTTAACCAG
AGATACCTGTCTCCCAAATACATCAAGATGTTTGTACTGGACGAAGCTGATGAAATGTTAAGCCATGGATTCAAGGACTC
AATCTATGACATATTCCAAAAGCTCAACAGCAACACCCAGGTGGTTTTGCTATCAGCCACAAGGCCTTCTGATGTGCTTG
AGGTGACCAAGAAGTTCATGAGGGACCCCATTTGGATTCCTGTCAAGAAGGAAGAGTTGACCCTGGAGGGGGTCCACCAA
TTCTACATCAATGTGGAACATGAGGAGTGGAAACTGACCACACTGTGTGACTTGTATAAAACCCTGATTGTCACCCTAGG
CAGTCATCTTCATCAATACTGGAAAGAAGGTGGATTGGCTC

Alignment

ORF - retro_ggor_537 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 82.69 %
• Parental protein coverage: 68.97 %
• Number of stop codons detected: 4
• Number of frameshifts detected: 3

Retrocopy - Parental Gene Alignment:

Parental  SRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAIL-PCIKGYDVIAQAQ
          SRDNGPD.MEPEG.IE.N.NEIVDSFDDMNL.ESLL.GIYAYGFEK.SAIQQRAIL..CIK.YDVI.QA.
Retrocopy SRDNGPDEMEPEGIIERN*NEIVDSFDDMNLLESLLHGIYAYGFEKSSAIQQRAIL<SCIKEYDVITQAL

Parental  SGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVR-AEVQK
          SG.GK.ATFAISILQ.IELDLKAT..LV.A.T..LAQ.I.KVVM.LGDYMGASCH.CIGGTNV..AE.QK
Retrocopy SGNGKMATFAISILQ*IELDLKATHTLVPAST**LAQKIEKVVMTLGDYMGASCHGCIGGTNVQ>AEMQK

Parental  LQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATM
          LQME.PHIIVGTPG...DMLN.RYLSPKYIKMFVLDEADEMLS.GFKD.IYDIFQKLNSNTQVVLLSAT.
Retrocopy LQMEVPHIIVGTPGHMSDMLNQRYLSPKYIKMFVLDEADEMLSHGFKDSIYDIFQKLNSNTQVVLLSATR

Parental  PSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQ-AVIFINTRRKV
          PSDVLEVTKKFMRDPI.I.VKKEELTLEG..QFYINVE.EEWKL.TLCDLY.TL..T..AVIFINT..KV
Retrocopy PSDVLEVTKKFMRDPIWIPVKKEELTLEGVHQFYINVEHEEWKLTTLCDLYKTLIVTL>AVIFINTGKKV

Parental  DWL
          DWL
Retrocopy DWL

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain_prefrontal_cortex	0.05 RPM	110.39 RPM
SRP007412_cerebellum	0.08 RPM	82.26 RPM
SRP007412_heart	0.00 RPM	47.25 RPM
SRP007412_kidney	0.04 RPM	206.00 RPM
SRP007412_liver	0.03 RPM	183.20 RPM
SRP007412_testis	0.00 RPM	224.09 RPM

ChIP-Seq

Gorilla gorilla was not studied using ChIP-Seq data.

EST

Gorilla gorilla was not studied using EST data.

TSS

Gorilla gorilla was not studied using FANTOM5 data.

Lab

retro_ggor_537 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ggor_537 has 3 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_610
Pan troglodytes	retro_ptro_450
Pongo abelii	retro_pabe_548

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Parental genes homology:

Parental genes homology involve 16 parental genes, and 67 retrocopies.

Species	Parental gene accession	Retrocopies number
Cavia porcellus	ENSCPOG00000004900	1 retrocopy	retro_cpor_1088,
Erinaceus europaeus	ENSEEUG00000003581	1 retrocopy	retro_eeur_610,
Felis catus	ENSFCAG00000024897	1 retrocopy	retro_fcat_370,
Homo sapiens	ENSG00000161960	10 retrocopies	retro_hsap_1004, retro_hsap_1182, retro_hsap_1252, retro_hsap_1270, retro_hsap_1796, retro_hsap_2520, retro_hsap_4836, retro_hsap_4873, retro_hsap_4931, retro_hsap_610,
Gorilla gorilla	ENSGGOG00000005867	2 retrocopies	retro_ggor_525, retro_ggor_712,
Gorilla gorilla	ENSGGOG00000024702	8 retrocopies	retro_ggor_1321, retro_ggor_1572, retro_ggor_3015, retro_ggor_537 , retro_ggor_798, retro_ggor_920, retro_ggor_983, retro_ggor_998,
Macropus eugenii	ENSMEUG00000010780	2 retrocopies	retro_meug_158, retro_meug_179,
Myotis lucifugus	ENSMLUG00000003415	3 retrocopies	retro_mluc_1047, retro_mluc_1229, retro_mluc_1805,
Macaca mulatta	ENSMMUG00000013499	1 retrocopy	retro_mmul_1280,
Monodelphis domestica	ENSMODG00000007090	3 retrocopies	retro_mdom_1282, retro_mdom_326, retro_mdom_711,
Mus musculus	ENSMUSG00000059796	7 retrocopies	retro_mmus_1810, retro_mmus_1853, retro_mmus_2320, retro_mmus_2473, retro_mmus_3122, retro_mmus_325, retro_mmus_527,
Nomascus leucogenys	ENSNLEG00000009547	7 retrocopies	retro_nleu_2109, retro_nleu_2117, retro_nleu_230, retro_nleu_2470, retro_nleu_2769, retro_nleu_440, retro_nleu_701,
Pan troglodytes	ENSPTRG00000008694	10 retrocopies	retro_ptro_1201, retro_ptro_1465, retro_ptro_2954, retro_ptro_3112, retro_ptro_3247, retro_ptro_450, retro_ptro_750, retro_ptro_810, retro_ptro_862, retro_ptro_870,
Rattus norvegicus	ENSRNOG00000030628	3 retrocopies	retro_rnor_1059, retro_rnor_2213, retro_rnor_2306,
Sorex araneus	ENSSARG00000006017	7 retrocopies	retro_sara_224, retro_sara_383, retro_sara_461, retro_sara_567, retro_sara_6, retro_sara_605, retro_sara_89,
Ictidomys tridecemlineatus	ENSSTOG00000024814	1 retrocopy	retro_itri_610,