RetrogeneDB ID: retro_cfam_32

Retrocopy location

• Organism: Dog (Canis familiaris)
• Coordinates: 21:32646853..32647216(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: ENSCAFG00000007241
• Aliases: None
• Status: KNOWN_PROTEIN_CODING

Parental gene information

• Parental gene symbol: CYB5A
• Ensembl ID: ENSCAFG00000000026
• Aliases: None
• Description: Canis lupus familiaris cytochrome b5 type A (microsomal) (CYB5A), mRNA. [Source:RefSeq mRNA;Acc:NM_001193298]

Sequence

>retro_cfam_32
GAAGAGATCCAGAAGCACAACCACAGCAAGAGCACCTGGTTGATCCTACACCACAAGGTGTACGATTTGACCAAGTTTTT
GGAGGAGCATCCTAGTGGGGAAGAAGTATTAAGGGAACAAGCTGGAGGGGATGCTACTGAAAATTTCGAGGACGTGGGGC
ACTCTACAGATGCTAGAGAATTGTCCAAAACATACATCGTTGGGGAACTTCATCCGGATGACAGAGCAAAGATAAACAAG
CCTTCGGAAACTCTCATTACTACTGTTGATTCTAATTCTAGCTGGTGGACCAACTGGGTGATCCCAGCCATCTCAGCACT
GGTGGTAGCCCTGATGTATCACTTCTACACAGCGGAAGATTAA

Alignment

ORF - retro_cfam_32 Open Reading Frame is conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 97.48 %
• Parental protein coverage: 74.38 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 0

Retrocopy - Parental Gene Alignment:

Parental  EEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDARELSKTFIIGEL
          EEIQKHNHSKSTWLILHHKVYDLTKFLEEHP.GEEVLREQAGGDATENFEDVGHSTDARELSKT.I.GEL
Retrocopy EEIQKHNHSKSTWLILHHKVYDLTKFLEEHPSGEEVLREQAGGDATENFEDVGHSTDARELSKTYIVGEL

Parental  HPDDRAKINKPSETLITTVDSNSSWWTNWVIPAISALVVALMYHFYTAE
          HPDDRAKINKPSETLITTVDSNSSWWTNWVIPAISALVVALMYHFYTAE
Retrocopy HPDDRAKINKPSETLITTVDSNSSWWTNWVIPAISALVVALMYHFYTAE

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012049_cerebellum	0.17 RPM	11.89 RPM
SRP017611_brain	0.00 RPM	8.57 RPM
SRP017611_kidney	0.07 RPM	151.09 RPM
SRP017611_liver	0.29 RPM	209.20 RPM

ChIP-Seq

Canis familiaris was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_cfam_32 retrocopy.

TSS

Canis familiaris was not studied using FANTOM5 data.

Lab

retro_cfam_32 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_cfam_32 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 27 parental genes, and 51 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000013614	2 retrocopies	retro_amel_1727, retro_amel_860,
Canis familiaris	ENSCAFG00000000026	2 retrocopies	retro_cfam_32 , retro_cfam_787,
Choloepus hoffmanni	ENSCHOG00000000359	2 retrocopies	retro_chof_128, retro_chof_239,
Callithrix jacchus	ENSCJAG00000003163	3 retrocopies	retro_cjac_2427, retro_cjac_2602, retro_cjac_686,
Cavia porcellus	ENSCPOG00000012111	1 retrocopy	retro_cpor_1140,
Equus caballus	ENSECAG00000017581	1 retrocopy	retro_ecab_12,
Erinaceus europaeus	ENSEEUG00000010683	1 retrocopy	retro_eeur_587,
Echinops telfairi	ENSETEG00000000691	1 retrocopy	retro_etel_381,
Homo sapiens	ENSG00000166347	3 retrocopies	retro_hsap_1307, retro_hsap_1373, retro_hsap_2462,
Gorilla gorilla	ENSGGOG00000007968	1 retrocopy	retro_ggor_1068,
Loxodonta africana	ENSLAFG00000010020	1 retrocopy	retro_lafr_414,
Macropus eugenii	ENSMEUG00000015899	1 retrocopy	retro_meug_1573,
Myotis lucifugus	ENSMLUG00000011462	5 retrocopies	retro_mluc_1159, retro_mluc_1333, retro_mluc_1475, retro_mluc_1701, retro_mluc_1908,
Macaca mulatta	ENSMMUG00000013607	3 retrocopies	retro_mmul_2162, retro_mmul_2199, retro_mmul_546,
Mustela putorius furo	ENSMPUG00000016630	1 retrocopy	retro_mputfur_776,
Mus musculus	ENSMUSG00000024646	1 retrocopy	retro_mmus_2177,
Nomascus leucogenys	ENSNLEG00000009232	1 retrocopy	retro_nleu_1066,
Otolemur garnettii	ENSOGAG00000000052	4 retrocopies	retro_ogar_1248, retro_ogar_1888, retro_ogar_2041, retro_ogar_2869,
Ochotona princeps	ENSOPRG00000011584	3 retrocopies	retro_opri_1091, retro_opri_296, retro_opri_405,
Pongo abelii	ENSPPYG00000009240	2 retrocopies	retro_pabe_1094, retro_pabe_1140,
Pan troglodytes	ENSPTRG00000010111	3 retrocopies	retro_ptro_1421, retro_ptro_898, retro_ptro_943,
Rattus norvegicus	ENSRNOG00000015205	2 retrocopies	retro_rnor_1565, retro_rnor_2455,
Ictidomys tridecemlineatus	ENSSTOG00000022436	1 retrocopy	retro_itri_1617,
Tupaia belangeri	ENSTBEG00000014998	3 retrocopies	retro_tbel_3229, retro_tbel_3338, retro_tbel_4600,
Tarsius syrichta	ENSTSYG00000002241	1 retrocopy	retro_tsyr_1033,
Tursiops truncatus	ENSTTRG00000015272	1 retrocopy	retro_ttru_1588,
Drosophila melanogaster	FBgn0029854	1 retrocopy	retro_dmel_39,