RetrogeneDB ID: retro_mmus_1160

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 14:5738321..5739180(+)
• Located in intron of: ENSMUSG00000096629

Retrocopy information

• Ensembl ID: ENSMUSG00000091044
• Aliases: None
• Status: KNOWN_PSEUDOGENE

Parental gene information

• Parental gene symbol: Esd
• Ensembl ID: ENSMUSG00000021996
• Aliases: Esd, Es-10, Es10, FGH, sid478
• Description: esterase D/formylglutathione hydrolase [Source:MGI Symbol;Acc:MGI:95421]

Sequence

>retro_mmus_1160
CCGAGGAGGAGAATGGCACTCAAAGAGATTTCCAGGAACAGGTGCTTTGGCGGGCTCCAGAAATTTTTCGAACATAGCAG
TGTTGAGCTGAAATGTAAAATGAAATTTGCTGTCTACCTACCACCCCAGGCAGAGAGTGGAAAGTGTCCAGCGCTTTACT
GGCTGTCTGGTTTAACTTACACAGAACAAAATTACATATCAAAGTCTGGCTATCAGCAAGCTGCCTCAGAGCCCGGCCTT
GTGGTCATTGCCCCTGATACCAGCCCTCGTGGCTGCAATATTAAAGGAGAAGATGACAGCTGGGACTTTGGCACTGGTGG
TGGGTTTTATGTGAATGCCACAGAAGATCCTTGGAAAGCTAACTACAGAATGTACTCTTACGTCACAGAGGAGCTTCCAC
AACTCATAAATGCCAATTTCCCAGTGGACCCACAGAGGATGGTCTATTTTTGGTCACTCCATGGGAGGCCACCGAGCTCT
GATTTGTGCTTTGAAAAATCTTGGAAAATACAGATCTGTGTCAGCATTTGCTCCAATTTGCAACCCTGTGCTGTGTCCTT
GGGGCAAAAAAACTTTTCGTGGATATTTGGGACCAGATGAAAGCAAATGGAAGGCATACGATGCGACCTGTCTCGTGAAG
TCATATTCGGGTTCCCAGATTGACATCCTAATTGATCAAGGAAAAGATGACGAGATTCTTTCAAATGGGCAGTTACTCCC
TGATAACTTCATAGCTGCCTGCACAGAGAAGAAAATCCCTGTTGTTTTTAGGCTACAAGAGGGTTATGATCATAGCTACT
GCTTCATCGCAACCTTCATCGCTGACCACATCAGACACCATGCTAAGTACCTGAATGCA

Alignment

ORF - retro_mmus_1160 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 94.08 %
• Parental protein coverage: 96.95 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  PRRRMALKQISSNRCFGGLQKVFEHSSVELKCKMRFAVYLPPQAESGKCPALYWLSGLTCTEQNFISKSG
          PRRRMALK.IS.NRCFGGLQK.FEHSSVELKCKM.FAVYLPPQAESGKCPALYWLSGLT.TEQN.ISKSG
Retrocopy PRRRMALKEISRNRCFGGLQKFFEHSSVELKCKMKFAVYLPPQAESGKCPALYWLSGLTYTEQNYISKSG

Parental  YQQAASEHGLVVIAPDTSPRGCNIKGEDDSWDFGTGAGFYVNATEDPWKANYRMYSYVTEELPQLINANF
          YQQAASE.GLVVIAPDTSPRGCNIKGEDDSWDFGTG.GFYVNATEDPWKANYRMYSYVTEELPQLINANF
Retrocopy YQQAASEPGLVVIAPDTSPRGCNIKGEDDSWDFGTGGGFYVNATEDPWKANYRMYSYVTEELPQLINANF

Parental  PVDPQRM-SIFGHSMGGHGALICALKNPGKYRSVSAFAPICNPVLCSWGKKAFSGYLGPDESKWKAYDAT
          PVDPQRM.SIFGHSMGGH.ALICALKN.GKYRSVSAFAPICNPVLC.WGKK.F.GYLGPDESKWKAYDAT
Retrocopy PVDPQRM>SIFGHSMGGHRALICALKNLGKYRSVSAFAPICNPVLCPWGKKTFRGYLGPDESKWKAYDAT

Parental  CLVKAYSGSQIDILIDQGKDDEFLSNGQLLPDNFIAACTEKKIPVVFRLQEGYDHSYYFIATFIADHIRH
          CLVK.YSGSQIDILIDQGKDDE.LSNGQLLPDNFIAACTEKKIPVVFRLQEGYDHSY.FIATFIADHIRH
Retrocopy CLVKSYSGSQIDILIDQGKDDEILSNGQLLPDNFIAACTEKKIPVVFRLQEGYDHSYCFIATFIADHIRH

Parental  HAKYLNA
          HAKYLNA
Retrocopy HAKYLNA

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	34.41 RPM
SRP007412_cerebellum	0.00 RPM	20.84 RPM
SRP007412_heart	0.00 RPM	37.70 RPM
SRP007412_kidney	0.00 RPM	126.60 RPM
SRP007412_liver	0.00 RPM	101.26 RPM
SRP007412_testis	0.00 RPM	3.70 RPM

ChIP-Seq

RNA Polymerase II activity near the 5' end of retro_mmus_1160 was not detected

EST

No EST(s) were mapped for retro_mmus_1160 retrocopy.

TSS

No TSS is located nearby retro_mmus_1160 retrocopy 5' end.

Lab

retro_mmus_1160 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_1160 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 22 parental genes, and 52 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000013377	2 retrocopies	retro_amel_1085, retro_amel_1803,
Bos taurus	ENSBTAG00000003415	1 retrocopy	retro_btau_908,
Canis familiaris	ENSCAFG00000004473	2 retrocopies	retro_cfam_1381, retro_cfam_1762,
Callithrix jacchus	ENSCJAG00000009380	2 retrocopies	retro_cjac_24, retro_cjac_257,
Cavia porcellus	ENSCPOG00000011089	3 retrocopies	retro_cpor_1253, retro_cpor_1396, retro_cpor_568,
Dasypus novemcinctus	ENSDNOG00000017553	2 retrocopies	retro_dnov_2502, retro_dnov_96,
Equus caballus	ENSECAG00000024350	1 retrocopy	retro_ecab_808,
Felis catus	ENSFCAG00000026826	2 retrocopies	retro_fcat_1641, retro_fcat_812,
Loxodonta africana	ENSLAFG00000006901	1 retrocopy	retro_lafr_843,
Macaca mulatta	ENSMMUG00000004209	1 retrocopy	retro_mmul_1013,
Monodelphis domestica	ENSMODG00000011290	2 retrocopies	retro_mdom_1607, retro_mdom_1608,
Mustela putorius furo	ENSMPUG00000001494	2 retrocopies	retro_mputfur_1339, retro_mputfur_543,
Mus musculus	ENSMUSG00000021996	9 retrocopies	retro_mmus_1159, retro_mmus_1160 , retro_mmus_1161, retro_mmus_1162, retro_mmus_1163, retro_mmus_1166, retro_mmus_1168, retro_mmus_1236, retro_mmus_1237,
Oryctolagus cuniculus	ENSOCUG00000009968	1 retrocopy	retro_ocun_709,
Otolemur garnettii	ENSOGAG00000010048	1 retrocopy	retro_ogar_162,
Ochotona princeps	ENSOPRG00000011461	2 retrocopies	retro_opri_230, retro_opri_845,
Procavia capensis	ENSPCAG00000004402	1 retrocopy	retro_pcap_354,
Pteropus vampyrus	ENSPVAG00000009910	3 retrocopies	retro_pvam_1309, retro_pvam_299, retro_pvam_87,
Rattus norvegicus	ENSRNOG00000009512	2 retrocopies	retro_rnor_1053, retro_rnor_1593,
Sarcophilus harrisii	ENSSHAG00000009581	3 retrocopies	retro_shar_307, retro_shar_337, retro_shar_570,
Ictidomys tridecemlineatus	ENSSTOG00000002854	2 retrocopies	retro_itri_182, retro_itri_639,
Tupaia belangeri	ENSTBEG00000005120	7 retrocopies	retro_tbel_1621, retro_tbel_1776, retro_tbel_2, retro_tbel_296, retro_tbel_3059, retro_tbel_3072, retro_tbel_3698,