RetrogeneDB ID: retro_mdom_427

Retrocopy location

• Organism: Opossum (Monodelphis domestica)
• Coordinates: 1:489123618..489125338(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: ENSMODG00000016407
• Aliases: None
• Status: KNOWN_PSEUDOGENE

Parental gene information

• Parental gene symbol: HSPD1
• Ensembl ID: ENSMODG00000012289
• Aliases: None
• Description: heat shock 60kDa protein 1 (chaperonin) [Source:HGNC Symbol;Acc:5261]

Sequence

>retro_mdom_427
ATGCTCCGGTTGTCCATAGTCCTTCGGCAGATAAGGCCAGTGTCTAGGGCACTGGCCCCTCACTTGACACGTGCTTATGC
TAAAGATGTCAAATTTGGAGCAGATGCCAGAGCCTTAATGCTTCAAGGTGTGGATCTTTTAGTTGACACTGTTGCTGTTA
CTATGGGGCCAAAGGGGAGAACTGGGATCATTGAACAGAGTTGGGGAAGTCCCAAGGTCACAAAAGATGGAGTGACTGTT
GCAAAAGCAATTGACTTGAAGGACAAATATAAAAACATTGGTGCCTGACTAGTTCAGGACATTGCCAATAACACAAATGA
AGAGGCTGGGGATGGACTACCACTGCCACAGTCCTTGCACGCTCCATTGCTAAGGAAGGTTTTGAGAAGATCAGCAAAGG
AGCAAATCCAGTGGAGATCAGGAGAGGTGTGATGTTGGCTGTTGACGCTGTCATTAGTGAGCTTAAGAACCAGTCTAAAC
TTGTCACAACCCCTGAAGAAATTGCTCAGGTCGCTACAATTTCTGCAAATGGAGATCAAGAAATTGGCAACATAATATCT
GATGCAATGAAAAAAGTTGGACGAAAAGGTGTCATCACAGTAAAGGATGGAAAAACATTGAATGACAAATTAGAAATTAT
TGAAGGCATGAAATTTGACAGAGGTTATATCTCTCCTTATTTTATTAATACAGCTAAAGGTCAGAAATGTGAATTCCAGG
ATGCCTATGTTCTCCTAAGTGAAAAAAAAAATCCCCAGTGTCCAATCTATTGTACCTGCACTTGAAATTGCAAATGCCCA
CCGTAAGCCTTTAGTAATAATTGCTGAAGATGTTGATGGAGAAGCATTAAGTACTCTTGTTTTGAACAGGTTAAAAGTTG
GACTGCAGGTTGTTGCAGTGAAAGCTCCAGGTTTTGATGATAACAGGAAGAACCAGCTTAAGGATATGGCTATTGCTACT
GGTGGTTCAGTATTTGGAGAAGAGGGATTGACTCTGAAATCTTGAAGATATTCAAGCTCATGATTTTGGAAAAGTCGGGG
AGGTTATTGTGACAAAAGATGATGCCATGCTTTTGAAGGGAAGGGGTGACAGATCCCAAATTGAAAAACGTGTTCAGGAA
ATCATTGAACAGTTAGAGATTACTACTAGTGACTATGAAAAAGAAAAGTTAAATGAGCGATTGGCCAAACTTTCTGACGG
GGTTGCTGTAATAAAGGTTGGTGGAACAAGTGACGTAGAAGTGAATGAAAAGAAAGACCGAGTTACTGATGCCCTTAATG
CTACACGTGCTGCTGTTGAAGAGGGCATAGTTCTGGGAGGTGGCTGTGCATTGCTTCGATGCATTCCAGCCTTAGAAGCA
TTAATTCCAAATAATGAAGATCAAAAAATTGGAATAGACATAATTAAGAAGACACTGAAAATCCCTGCAATGACCATTGC
TAAGAATGCTGGTGTGGAAGGCTCATTGATAGTTGAGAAGATTTTACAAAGCTCTTCAGAGATAGGTTATGATGCCATGA
TTGGAGATTTTATGAATATGGTTGAAAAAGGAATAATTGACCCAACTAAGGTTATAAGAACTGCTCTGTTGGATGCTGCT
GGTGTGGCTTCTTTATTAACTACAGCAGAAGTTGTTGTTACTGAAATTCCTAAAGAAGAGAAAGAACCTGGCATGGGAGG
GATGGATGGTATGGATGGAGGCATGGGAAGTGGCATGTTC

Alignment

ORF - retro_mdom_427 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 95.31 %
• Parental protein coverage: 100. %
• Number of stop codons detected: 1
• Number of frameshifts detected: 3

Retrocopy - Parental Gene Alignment:

Parental  MLRLSTVLRQIRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGS
          MLRLS.VLRQIRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLL.D.VAVTMGPKGRT.IIEQSWGS
Retrocopy MLRLSIVLRQIRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLVDTVAVTMGPKGRTGIIEQSWGS

Parental  PKVTKDGVTVAKAIDLKDKYKNIGARLVQDVANNTNEEAGDG-TTTATVLARSIAKEGFEKISKGANPVE
          PKVTKDGVTVAKAIDLKDKYKNIGA.LVQD.ANNTNEEAGDG.TTTATVLARSIAKEGFEKISKGANPVE
Retrocopy PKVTKDGVTVAKAIDLKDKYKNIGA*LVQDIANNTNEEAGDG<TTTATVLARSIAKEGFEKISKGANPVE

Parental  IRRGVMLAVDAVISELKNQSKPVTTPEEIAQVATISANGDREIGNIISDAMKKVGRKGVITIQDGKTLND
          IRRGVMLAVDAVISELKNQSK.VTTPEEIAQVATISANGD.EIGNIISDAMKKVGRKGVIT..DGKTLND
Retrocopy IRRGVMLAVDAVISELKNQSKLVTTPEEIAQVATISANGDQEIGNIISDAMKKVGRKGVITVKDGKTLND

Parental  ELEIIEGMKFDRGYISPYFINTAKGQKCEFQDAYVLLSEKKIS-SVQSIVPALEIANAHRKPLVIIAEDV
          .LEIIEGMKFDRGYISPYFINTAKGQKCEFQDAYVLLS......SVQSIVPALEIANAHRKPLVIIAEDV
Retrocopy KLEIIEGMKFDRGYISPYFINTAKGQKCEFQDAYVLLSXXXXP>SVQSIVPALEIANAHRKPLVIIAEDV

Parental  DGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGTVFGEEGLTL-NLEDIQAHDFGKVGE
          DGEALSTLVLNRLKVGLQVVAVKAPGF.DNRKNQLKDMAIATGG.VFGEEGLTL.NLEDIQAHDFGKVGE
Retrocopy DGEALSTLVLNRLKVGLQVVAVKAPGFDDNRKNQLKDMAIATGGSVFGEEGLTL>NLEDIQAHDFGKVGE

Parental  VIVTKDDAMLLKGRGDRSQIEKRVQEIIEQLEITTSDYEKEKLNERLAKLSDGVAVIKVGGTSDVEVNEK
          VIVTKDDAMLLKGRGDRSQIEKRVQEIIEQLEITTSDYEKEKLNERLAKLSDGVAVIKVGGTSDVEVNEK
Retrocopy VIVTKDDAMLLKGRGDRSQIEKRVQEIIEQLEITTSDYEKEKLNERLAKLSDGVAVIKVGGTSDVEVNEK

Parental  KDRVTDALNATRAAVEEGIVLGGGCALLRCIPALEALTPNNEDQKIGIDIIKKTLKIPAMTIAKNAGVEG
          KDRVTDALNATRAAVEEGIVLGGGCALLRCIPALEAL.PNNEDQKIGIDIIKKTLKIPAMTIAKNAGVEG
Retrocopy KDRVTDALNATRAAVEEGIVLGGGCALLRCIPALEALIPNNEDQKIGIDIIKKTLKIPAMTIAKNAGVEG

Parental  SLIVEKILQSSSEIGYDAMIGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKEPG
          SLIVEKILQSSSEIGYDAMIGDF.NMVEKGIIDPTKV.RTALLDAAGVASLLTTAEVVVTEIPKEEKEPG
Retrocopy SLIVEKILQSSSEIGYDAMIGDFMNMVEKGIIDPTKVIRTALLDAAGVASLLTTAEVVVTEIPKEEKEPG

Parental  MGGMGGMGGGMGGGMF
          MGGM.GM.GGMG.GMF
Retrocopy MGGMDGMDGGMGSGMF

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	17.64 RPM	0.00 RPM
SRP007412_cerebellum	7.83 RPM	0.00 RPM
SRP007412_heart	18.94 RPM	0.00 RPM
SRP007412_kidney	43.46 RPM	0.00 RPM
SRP007412_liver	29.71 RPM	0.00 RPM
SRP007412_testis	9.63 RPM	0.00 RPM

ChIP-Seq

Monodelphis domestica was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_mdom_427 retrocopy.

TSS

Monodelphis domestica was not studied using FANTOM5 data.

Lab

retro_mdom_427 was not experimentally validated.

Homology

Retrocopy orthology:

Monodelphis domestica does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 25 parental genes, and 94 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000013770	1 retrocopy	retro_amel_771,
Bos taurus	ENSBTAG00000012586	1 retrocopy	retro_btau_988,
Canis familiaris	ENSCAFG00000010865	4 retrocopies	retro_cfam_1185, retro_cfam_1620, retro_cfam_2165, retro_cfam_614,
Choloepus hoffmanni	ENSCHOG00000007401	5 retrocopies	retro_chof_1105, retro_chof_1300, retro_chof_1822, retro_chof_2000, retro_chof_2397,
Callithrix jacchus	ENSCJAG00000003990	6 retrocopies	retro_cjac_1516, retro_cjac_1534, retro_cjac_1975, retro_cjac_3172, retro_cjac_3400, retro_cjac_4096,
Dasypus novemcinctus	ENSDNOG00000013226	3 retrocopies	retro_dnov_1990, retro_dnov_2113, retro_dnov_2512,
Echinops telfairi	ENSETEG00000009184	4 retrocopies	retro_etel_1440, retro_etel_426, retro_etel_885, retro_etel_925,
Homo sapiens	ENSG00000144381	7 retrocopies	retro_hsap_1006, retro_hsap_2770, retro_hsap_2987, retro_hsap_3151, retro_hsap_3494, retro_hsap_3930, retro_hsap_4018,
Gorilla gorilla	ENSGGOG00000008267	6 retrocopies	retro_ggor_1927, retro_ggor_2069, retro_ggor_2361, retro_ggor_2698, retro_ggor_800, retro_ggor_985,
Loxodonta africana	ENSLAFG00000000053	1 retrocopy	retro_lafr_476,
Macropus eugenii	ENSMEUG00000011987	2 retrocopies	retro_meug_1688, retro_meug_224,
Myotis lucifugus	ENSMLUG00000000871	4 retrocopies	retro_mluc_1458, retro_mluc_2280, retro_mluc_2348, retro_mluc_601,
Macaca mulatta	ENSMMUG00000010489	1 retrocopy	retro_mmul_83,
Monodelphis domestica	ENSMODG00000012289	3 retrocopies	retro_mdom_427 , retro_mdom_493, retro_mdom_756,
Mustela putorius furo	ENSMPUG00000007818	3 retrocopies	retro_mputfur_1118, retro_mputfur_245, retro_mputfur_434,
Mus musculus	ENSMUSG00000025980	5 retrocopies	retro_mmus_1225, retro_mmus_2494, retro_mmus_3206, retro_mmus_439, retro_mmus_706,
Nomascus leucogenys	ENSNLEG00000006874	7 retrocopies	retro_nleu_1349, retro_nleu_2238, retro_nleu_229, retro_nleu_657, retro_nleu_675, retro_nleu_691, retro_nleu_787,
Otolemur garnettii	ENSOGAG00000034053	5 retrocopies