RetrogeneDB ID: retro_mmus_756

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 11:4129374..4130066(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: ENSMUSG00000083838
• Aliases: None
• Status: KNOWN_PSEUDOGENE

Parental gene information

• Parental gene symbol: Tra2b
• Ensembl ID: ENSMUSG00000022858
• Aliases: None
• Description: transformer 2 beta homolog (Drosophila) [Source:MGI Symbol;Acc:MGI:106016]

Sequence

>retro_mmus_756
AGGTCTAGATCCAGAAGAAGTTCTAGAAGGCATTATACAAGGTCACGATCACGATCTCGCTCGCATAGACGATCCGGAGC
AGGTCTTACAGCTGAGATTATGGCAGGCGCCACAGCCACAGCCATTCTCCCATGTCTACTCGAAGGCGTCATGTTGGGAA
TCGGGCAAATCCTGACCCCAACTGTTGTCTTGGCGTGTTTGGGTTGAGCTTATACAGCACAGAAAGAGACCTAAGAGAAG
TGTTCTCTAACTATGGCCCCATTGCTGATGTGTCTATTGTATATGACCAGCAATCTAGACGTTCAAGAGGATTTGCCTTT
GTATATTTTGAAAATGTGGATGATGCCAAGGAAGCTAAAGAACATGCCAGTGGAATGGAGCTTGATGGGCGACGAATTAG
AGTCGATTTCTCTATAACAAAAAGGCCCCATACCCCAACACCAGGAATTTATATGGGGAGAGCCATTTATGGCAGCTCAC
ACCGCCGAGACTATTATGACAGAGGATACGATCAGGGGTATGATGGCGGGGGCTATTACAGCAGATCACACAGAGGAGGA
GGTGGGGGAGGTGGATGGAGAGCAGCTCAAGACAGGGATGAGACTTACAGAAGACGGTCACCTTCTCCTTACTACAGTCG
TGGAGGATACAGGTCACATTCTCCATCACGATCCTACTCACCTCGTCGCTAC

Alignment

ORF - retro_mmus_756 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 91.42 %
• Parental protein coverage: 80.56 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  RSRSRRSSRRHYTRSRSRSRSHRRS-RSRSYSRDYRRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGL
          RSRSRRSSRRHYTRSRSRSRSHRRS.RSRSYS.DY.RRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGL
Retrocopy RSRSRRSSRRHYTRSRSRSRSHRRS<RSRSYS*DYGRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGL

Parental  SLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSI
          SLY.TERDLREVFS.YGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKE.A.GMELDGRRIRVDFSI
Retrocopy SLYSTERDLREVFSNYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKEHASGMELDGRRIRVDFSI

Parental  TKRPHTPTPGIYMGRPTYGSSRRRDYYDRGYDRGYDDRDYYSRSYRGGGGGGGGWRAAQDRDQIYRRRSP
          TKRPHTPTPGIYMGR..YGSS.RRDYYDRGYD.GYD...YYSRS.R.GGGGGGGWRAAQDRD..YRRRSP
Retrocopy TKRPHTPTPGIYMGRAIYGSSHRRDYYDRGYDQGYDGGGYYSRSHR-GGGGGGGWRAAQDRDETYRRRSP

Parental  SPYYSRGGYRSRSRSRSYSPRRY
          SPYYSRGGYRS.S.SRSYSPRRY
Retrocopy SPYYSRGGYRSHSPSRSYSPRRY

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.05 RPM	36.47 RPM
SRP007412_cerebellum	0.09 RPM	41.08 RPM
SRP007412_heart	0.09 RPM	31.25 RPM
SRP007412_kidney	0.10 RPM	45.26 RPM
SRP007412_liver	0.05 RPM	29.14 RPM
SRP007412_testis	0.00 RPM	82.19 RPM

ChIP-Seq

RNA Polymerase II activity near the 5' end of retro_mmus_756 was not detected

EST

No EST(s) were mapped for retro_mmus_756 retrocopy.

TSS

No TSS is located nearby retro_mmus_756 retrocopy 5' end.

Lab

retro_mmus_756 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_756 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 9 parental genes, and 24 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000001697	2 retrocopies	retro_btau_1245, retro_btau_850,
Myotis lucifugus	ENSMLUG00000005781	2 retrocopies	retro_mluc_2565, retro_mluc_2567,
Mustela putorius furo	ENSMPUG00000003157	1 retrocopy	retro_mputfur_673,
Mus musculus	ENSMUSG00000022858	6 retrocopies	retro_mmus_1187, retro_mmus_2253, retro_mmus_2313, retro_mmus_3139, retro_mmus_3617, retro_mmus_756 ,
Mus musculus	ENSMUSG00000031134	2 retrocopies	retro_mmus_170, retro_mmus_187,
Mus musculus	ENSMUSG00000031167	4 retrocopies	retro_mmus_2044, retro_mmus_2963, retro_mmus_3019, retro_mmus_501,
Ochotona princeps	ENSOPRG00000007990	2 retrocopies	retro_opri_24, retro_opri_930,
Rattus norvegicus	ENSRNOG00000001783	4 retrocopies	retro_rnor_1130, retro_rnor_1915, retro_rnor_2156, retro_rnor_2807,
Tarsius syrichta	ENSTSYG00000000388	1 retrocopy	retro_tsyr_1632,