RetrogeneDB ID: retro_mmus_3327

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 9:31935448..31936800(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: ENSMUSG00000091639
• Aliases: None
• Status: KNOWN_PSEUDOGENE

Parental gene information

• Parental gene symbol: Tuba1b
• Ensembl ID: ENSMUSG00000023004
• Aliases: Tuba1b, Tuba2
• Description: tubulin, alpha 1B [Source:MGI Symbol;Acc:MGI:107804]

Sequence

>retro_mmus_3327
ATGCGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCAGATCGGCAATGCCTGCTGGGAGCTCTACTGCCTGGA
ACATGGCATCCAGCCTGATGGCCAGATGCCAAGTGATAAGACCATTGGGGGAGGAGATGACTCCTTCAACACCTTCTTCA
GTGAGACAGGAGCTGGCAAGCATGTGCCCCGGGCAGTGTTTGTAGACCTGGAACCTACGGTCATCGATGAAGTTCGCACT
GGCACCTACCGCCAGCTCTTCCACCCTGAGCAGCTCATCACAGGCAAGGAGGATGCTGCCAATAACTATGCCCGTGGCCA
CTACACCATTGGCAAGGAGATCATTGACCTTGTCCTGGACAGGATTCGCAAGCTGGCTGACCAGTGCACAGGTCTCCAGG
GCTTCTTGGTTTTCCACAGCTTTGGCGGGGGAACTGGCTCTGGCTTCACCTCCCTGCTGATGGAGCGGCTCTCTGTGGAT
TACGGAAAGAAGTCCAAGCTGGAGTTCTCCATTTAACCAGCCCCCCAGGTTTCCACTGCTGTGGTTGAGCCCTACAATTC
CATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATATCTGTC
GTAGAAACCTCGACATTGAGCGTCCAACCTACACCAACCTTAACCGCCTTATTAGCCAGATTGTGTCTTCCATCACTGCT
TCCCTCAGATTTGATGGGCCCTGAATGTTGATCTGACAGAATTCCAGACCAACCTGGTACCCTATCCTCGCATCCACTTC
CCTCTGGCCACTTATGCCCCTGTCATCTCTGCTGAGAAAGCCTACCATGAGCAGCTTTCTGTAGCAGAGATCACCAATGC
CTGCTTTGAGCCAGCCAACCAGATGGTGAAATGTGACCCTCGCCATGGTAAATACATGGCTTGCTGCCTGCTGTACCGTG
GTGATGTAGTTCCCAAAGATGTCAATGCTGCCATCGCCACCATCAAGACCAAGCGCAGCATCCAGTTTGTAGACTGGTGC
CCCACTGGCTTCAAGGTTGGCATTAACTACCAGCCTCCCACTGTGGTACCCGGTGGTGACCTGGCCAAGGTGCAGAGAGC
TGTGTGCATGCTGAGCAACACCACAGCCATTGCTGAGGCCTGGGCTCGCCTAGATCACAAGTTTGATCTGATGTATGTCA
AGCGTGCCTTTGTGCACTGGTATGTGGGTGAGGGCATGGAGGAGGGTGAGTTCTCTGAGGCCTGTGAGGACATGGCTGCC
CTAGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTGGAAGGCGAGGGGGAGGAAGAAGGAGAGGAATAC

Alignment

ORF - retro_mmus_3327 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 99.12 %
• Parental protein coverage: 100.0 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
          MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
Retrocopy MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL

Parental  EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS
          EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS
Retrocopy EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS

Parental  FGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
          FGGGTGSGFTSLLMERLSVDYGKKSKLEFSI.PAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
Retrocopy FGGGTGSGFTSLLMERLSVDYGKKSKLEFSI*PAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY

Parental  DICRRNLDIERPTYTNLNRLISQIVSSITASLRFDG-ALNVDLTEFQTNLVPYPRIHFPLATYAPVISAE
          DICRRNLDIERPTYTNLNRLISQIVSSITASLRFDG.ALNVDLTEFQTNLVPYPRIHFPLATYAPVISAE
Retrocopy DICRRNLDIERPTYTNLNRLISQIVSSITASLRFDG<ALNVDLTEFQTNLVPYPRIHFPLATYAPVISAE

Parental  KAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPT
          KAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPT
Retrocopy KAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPT

Parental  GFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFS
          GFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMY.KRAFVHWYVGEGMEEGEFS
Retrocopy GFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYVKRAFVHWYVGEGMEEGEFS

Parental  EAREDMAALEKDYEEVGVDSVEGEGEEEGEEY
          EA.EDMAALEKDYEEVGVDSVEGEGEEEGEEY
Retrocopy EACEDMAALEKDYEEVGVDSVEGEGEEEGEEY

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.63 RPM	77.45 RPM
SRP007412_cerebellum	0.43 RPM	63.92 RPM
SRP007412_heart	0.22 RPM	36.14 RPM
SRP007412_kidney	0.13 RPM	16.90 RPM
SRP007412_liver	0.00 RPM	8.82 RPM
SRP007412_testis	0.32 RPM	8.14 RPM

ChIP-Seq

RNA Polymerase II activity near the 5' end of retro_mmus_3327 was not detected

EST

No EST(s) were mapped for retro_mmus_3327 retrocopy.

TSS

TSS No.	TSS Name	TSS expression level (Expr) in TPM range:
		no expression	0 < Expr ≤ 1	1 < Expr ≤ 5	5 < Expr ≤ 10	Expr > 10
TSS #1	TSS_149205	224 libraries	372 libraries	455 libraries	20 libraries	1 library

The graphical summary, for retro_mmus_3327 TSS expression levels > 0 TPM .
TSS expression levels were studied across 1072 TSS-CAGE libraries, based on FANTOM5 data.
The expression values were visualized using beanplot. If you have any doubts, how to read it, read more in Kampstra P (2008)

Lab

retro_mmus_3327 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_3327 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 14 parental genes, and 29 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000012244	1 retrocopy	retro_btau_402,
Choloepus hoffmanni	ENSCHOG00000008444	2 retrocopies	retro_chof_179, retro_chof_360,
Callithrix jacchus	ENSCJAG00000020874	2 retrocopies	retro_cjac_1603, retro_cjac_1861,
Felis catus	ENSFCAG00000028689	3 retrocopies	retro_fcat_1802, retro_fcat_254, retro_fcat_798,
Homo sapiens	ENSG00000123416	1 retrocopy	retro_hsap_808,
Macropus eugenii	ENSMEUG00000010779	2 retrocopies	retro_meug_1648, retro_meug_2174,
Macropus eugenii	ENSMEUG00000011167	4 retrocopies	retro_meug_1121, retro_meug_157, retro_meug_1616, retro_meug_1964,
Monodelphis domestica	ENSMODG00000015206	2 retrocopies	retro_mdom_1632, retro_mdom_348,
Mus musculus	ENSMUSG00000023004	6 retrocopies	retro_mmus_1562, retro_mmus_1607, retro_mmus_1742, retro_mmus_1987, retro_mmus_3297, retro_mmus_3327 ,
Mus musculus	ENSMUSG00000043091	1 retrocopy	retro_mmus_843,
Mus musculus	ENSMUSG00000067338	1 retrocopy	retro_mmus_3356,
Otolemur garnettii	ENSOGAG00000009435	1 retrocopy	retro_ogar_240,
Pan troglodytes	ENSPTRG00000012950	1 retrocopy	retro_ptro_2158,
Sus scrofa	ENSSSCG00000000190	2 retrocopies	retro_sscr_380, retro_sscr_702,