RetrogeneDB ID: retro_mmus_1384

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 15:19454754..19456352(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Phgdh
• Ensembl ID: ENSMUSG00000053398
• Aliases: Phgdh, 3-PGDH, 3PGDH, 4930479N23, A10, PGAD, PGD, PGDH, SERA
• Description: 3-phosphoglycerate dehydrogenase [Source:MGI Symbol;Acc:MGI:1355330]

Sequence

>retro_mmus_1384
ATGAGCTTCGCAAATCTGCACAAAATACTCATCAGTGACAGCCTGGACACCTGCTGCCGGAAGATCCTGCAAGATGGAGG
GCTGCAGATGGTGGAGAAGCAGAACTTGGCCAGGAGGAGCTGATAGCTGAACTCCAGGACTGTGAAGGCCTCATTGTCCG
GTCAGCTACTAAGGTCACTGCTGATGTCATCAATGCAGCAGAGAAGCTCCAGGTGGTGGGCAGGGCTGGCACAGGCGTGG
ACAATGTGGATCTGGAGGCTGCCACGAGGAAGGACATCCTAGTCATGGACACTCCCAACGGGAACAGCCTCAGTGCTACG
GAGCTCACCTGTGGGATGATCATGTGCCTGGCCAGGCAGATTCCCCAGGCAACGGCTTCGATAAAAGATGGCAAATGGGA
CCGGAAGAAGTTCATGGGGACAGAACTGAACGGGAAGACACTGGGAATTCTTGGCCTCAGCAGAATCGGAAGAGAGGTGG
TCACCCGAATTCAGTCCTTTGGAATGAAGACTGTAGGCTATGACCCCATCATCTCTCCTGAAGTCGCGGCCTCCTTTGGT
GTTCAGCAGCTGCCTCTGGAGGAGATCTGGCCTCTCTGTGACTTTATAACTGTCCATACCCCACTCCTGCCCTCTACCAC
AGGCTTGCTGAATGACAGCACCTTTGCTCAGTGCAAGAAAGGCATGCGTGTGGTGAACTGTGCTCGAGGAGGCATTGTGG
ATAAAGGTGCCCTGCTGCGTGCCCTGCAGTCTGGTCAGTGTGCTGGGGCTGCACTGGATGTGTTTACAGAAGCGCCACCA
CAGGACCGGGCCTTAGTGGACCACGAGAATGTCATCAGCTGTCCCCACCTGGGTGCCAGCACCAAGGAAGCCCAGAGCCG
CTGTGGGGAGGAAATCGCAGTCCAGTTTGTGGACATGGTGAAGGGGAAATCTCTAACAGGTGTTGTGAACGCCCAGGCCC
TCACCATTGCCTTCTCTCTACACACCAAGCCTTGAATTGGTCTGGCAGAAGCATTGGGCACGCTGATGCACGCCTGGGCT
GGCTCCCCTAAAGGGACCATCCAGGTGGTTACACAAGGAACATCTCTGAAGAATGCTGGGACCTGCCTGAGCCCTGCAGT
CATTTTCGGCCTTCCGAGAGAAGCATCTAATTAGGCAGACGTGAACTTGGTGAACGCTAAGCTACTGGTGAAAGAGGCTG
GCCTCAAAGTCACCACCTCCCACAACCCTAGTGTTCCAGGGGAGCAGGGCAGCGGGGAATGCCTCCTGACTATGGCCCTG
GCAGGTGCCCCCTACCAAGCTGTGGGCTTGATCCAGGGCACCACACCAATGCTGCAGATGCTCAACGGAGCTGTCTTCAG
ACCAGAGGTGCCACTACACAGGGGCCAACCCCTGCTCATATTCCGGGATCAGCCCTCCAACCCTGTGATGCTGCCCACTA
TGATCGGCCTTCTGGCAGAGGCGGGTGTACAGCTGCTGTCCTACCAAACCTCCATGGTGTCTGACGGAGAGCCCTGGCAC
GTAATGGGCCTGTCCTCCCTGCTGCCCAGCCTGGAAACATGGAAGCAGCATGTATTGGAGACTTTCCAGTTCTGCTTC

Alignment

ORF - retro_mmus_1384 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 93.07 %
• Parental protein coverage: 100. %
• Number of stop codons detected: 2
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSK-EELIAELQDCEGLIVRSATKVTADVINAAEK
          M.FANL.K.LISDSLD.CCRKILQDGGLQ.VEKQNL...EELIAELQDCEGLIVRSATKVTADVINAAEK
Retrocopy MSFANLHKILISDSLDTCCRKILQDGGLQMVEKQNLAR<EELIAELQDCEGLIVRSATKVTADVINAAEK

Parental  LQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWDRKKFM
          LQVVGRAGTGVDNVDLEAATRK.ILVM.TPNGNSLSA.ELTCGMIMCLARQIPQATAS.KDGKWDRKKFM
Retrocopy LQVVGRAGTGVDNVDLEAATRKDILVMDTPNGNSLSATELTCGMIMCLARQIPQATASIKDGKWDRKKFM

Parental  GTELNGKTLGILGLGRIGREVATRMQSFGMKTVGYDPIISPEVAASFGVQQLPLEEIWPLCDFITVHTPL
          GTELNGKTLGILGL.RIGREV.TR.QSFGMKTVGYDPIISPEVAASFGVQQLPLEEIWPLCDFITVHTPL
Retrocopy GTELNGKTLGILGLSRIGREVVTRIQSFGMKTVGYDPIISPEVAASFGVQQLPLEEIWPLCDFITVHTPL

Parental  LPSTTGLLNDSTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVI
          LPSTTGLLNDSTFAQCKKG.RVVNCARGGIVD.GALLRALQSGQCAGAALDVFTE.PP.DRALVDHENVI
Retrocopy LPSTTGLLNDSTFAQCKKGMRVVNCARGGIVDKGALLRALQSGQCAGAALDVFTEAPPQDRALVDHENVI

Parental  SCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEAMGTLMHAWAGS
          SCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALT.AFS.HTKP.IGLAEA.GTLMHAWAGS
Retrocopy SCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTIAFSLHTKP*IGLAEALGTLMHAWAGS

Parental  PKGTIQVVTQGTSLKNAGTCLSPAVIVGLLREASKQADVNLVNAKLLVKEAGLNVTTSHNPGVPGEQGSG
          PKGTIQVVTQGTSLKNAGTCLSPAVI.GL.REAS..ADVNLVNAKLLVKEAGL.VTTSHNP.VPGEQGSG
Retrocopy PKGTIQVVTQGTSLKNAGTCLSPAVIFGLPREASN*ADVNLVNAKLLVKEAGLKVTTSHNPSVPGEQGSG

Parental  ECLLTVALAGAPYQAVGLVQGTTPMLQMLNGAVFRPEVPLRRGQPLLVFRAQPSDPGMLPTMIGLLAEAG
          ECLLT.ALAGAPYQAVGL.QGTTPMLQMLNGAVFRPEVPL.RGQPLL.FR.QPS.P.MLPTMIGLLAEAG
Retrocopy ECLLTMALAGAPYQAVGLIQGTTPMLQMLNGAVFRPEVPLHRGQPLLIFRDQPSNPVMLPTMIGLLAEAG

Parental  VQLLSYQTSMVSDGEPWHVMGLSSLLPSLETWKQHVLEAFQFCF
          VQLLSYQTSMVSDGEPWHVMGLSSLLPSLETWKQHVLE.FQFCF
Retrocopy VQLLSYQTSMVSDGEPWHVMGLSSLLPSLETWKQHVLETFQFCF

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	21.05 RPM
SRP007412_cerebellum	0.04 RPM	11.94 RPM
SRP007412_heart	0.00 RPM	0.59 RPM
SRP007412_kidney	0.00 RPM	5.64 RPM
SRP007412_liver	0.00 RPM	0.08 RPM
SRP007412_testis	3.34 RPM	7.27 RPM

ChIP-Seq

RNA Polymerase II actvity near the 5' end of retro_mmus_1384 was not detected

EST

No EST(s) were mapped for retro_mmus_1384 retrocopy.

TSS

No TSS is located nearby retro_mmus_1384 retrocopy 5' end.

Lab

retro_mmus_1384 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_1384 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 4 parental genes, and 22 retrocopies.

Species	Parental gene accession	Retrocopies number
Callithrix jacchus	ENSCJAG00000019916	4 retrocopies	retro_cjac_2894, retro_cjac_2958, retro_cjac_3441, retro_cjac_3994,
Myotis lucifugus	ENSMLUG00000002986	1 retrocopy	retro_mluc_2502,
Mus musculus	ENSMUSG00000053398	16 retrocopies	retro_mmus_1008, retro_mmus_1223, retro_mmus_1384 , retro_mmus_1708, retro_mmus_1835, retro_mmus_1892, retro_mmus_2057, retro_mmus_2169, retro_mmus_2889, retro_mmus_2979, retro_mmus_3021, retro_mmus_3176, retro_mmus_3180, retro_mmus_3217, retro_mmus_3441, retro_mmus_545,
Rattus norvegicus	ENSRNOG00000019328	1 retrocopy	retro_rnor_452,