RetrogeneDB ID: retro_mmul_848

Retrocopy location

• Organism: Rhesus macaque (Macaca mulatta)
• Coordinates: 11:19837411..19838785(-)
• Located in intron of: ENSMMUG00000016243

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: MMU.11091
• Ensembl ID: ENSMMUG00000004852
• Aliases: None
• Description: eukaryotic translation elongation factor 1 alpha 1 [Source:RefSeq peptide;Acc:NP_001182679]

Sequence

>retro_mmul_848
CAGGAAAAGACTCATATCAACATTGATTTCACTGGACACGTAGATTCGGACAAGTTCACCACTACTCGCCATCTGAACTA
CAAATGTGGTAGCATCGACAAAAGCAGCACTGAAAAATTTGAGAAGGAGGCTGCTGAGATGGGAAAGTGCTCTTTCAAGT
AGGCCTGGGTCTTGGATAAACTCAAAGCTGAGTGTGAATGTGGTATCACCATTGATATCTCCCTGCGGAAATTTGAGACC
AGCAAGTACTACGTGAGTATCACCGATGCCCCAGAACACAGAGACGTCACCAAAAACATGATTACAGGGACATCTCAGGC
TGACCGTGCTGTCCTGATTGTTGCTGCTGGTGTTGGTGATTTGCAGCCGGTATCTCCAAGAATGGACAGACCTAGGAGCA
TGCCCTTCTGGCTTACACACTGGAGGTGAAACAACTCATTGTTAGTGTTAACAAAATGGATTCCACCGAGCCACCCTACA
GCCAGAAGAGATATGAGGAAGCTGTTAAGGAAGTCAGCACTTACATTAAGAAAACTGGCTACAACCCCAACACAGCAGCA
TTTGTGCCAATTTCTGGCTGGAATGGTGACAACATGTGGGAGCCAAATGCTAACATGCCTTGGTTCAAGGGATGGAAAAC
CACTCGTAAGGATGGCAATACCAGTGGAACCACGCTGCTTGGCGCTCTGGACTGCATCCTACCACCAACTCGTCCAACTA
ACAAGCCCCTTTGCCTGCCTCTCCAGGATGTCTACAGAAAGTGGTGGTATTGGTACTGTTCCTGTTGGCTGAGTGCAGAC
TGGTGTTCTCAAACCTGGTATGGTGGTCACCTTTGCTCCAGTCAATGTTACAACTGAAGTAAAATCTGTCGAAATGCACC
ATGAAGCTTTGAGGAAAGCTCTTCCTGGGGACAATGTGGGCTTCAATGTCAAGAATGTGTCTGTGAAGGATGTTCATCAC
AGCAATGATGCTGGTGACAGCAAAAACGACCCACCAATGGAAGCAGCTGGCTTCACTGCTCAGGGGATTATCCTGAACCA
TCCAGGCCAAATAAGTGCTGGCTATGCCCCTGTACTGGATTGCCACACGGCTCACATTGCATGCAAGTTTGCCGAGCTGA
AGGAAAAGACTGATCACCTTTCTGGTGAAAAGCTGGAAGATGGCCCTAACTTCTTGAACTCTGGTGATGCTGCCATTGTT
GATATGGTTCCTGGCAAGCCCATGTATGTTGAGTGCTTCTCAGACTATCCACTTCTGGGTCACTTTGCTGTTCGTGGTAT
GAGACAGACTGTTGTGGTAGATGTCATCAAAGCAGTGAACAAGAAGGCTGCTGGAGCTGGCAAGGTCACCAAATCTGCCC
AGAAAGCTCAAAAG

Alignment

ORF - retro_mmul_848 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 85.65 %
• Parental protein coverage: 99.13 %
• Number of stop codons detected: 3
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGIT
          .EKTHINI...GHVDS.K.TTT.HL.YKCG.IDK...EKFEKEAAEMGK.SFK.AWVLDKLKAE.E.GIT
Retrocopy QEKTHINIDFTGHVDSDKFTTTRHLNYKCGSIDKSSTEKFEKEAAEMGKCSFK*AWVLDKLKAECECGIT

Parental  IDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGE-FEAGISKNGQTREHALLAY
          IDISL.KFETSKYYV.I.DAP.HRD..KNMITGTSQAD.AVLIVAAGVG..F.AGISKNGQT.EHALLAY
Retrocopy IDISLRKFETSKYYVSITDAPEHRDVTKNMITGTSQADRAVLIVAAGVGD<FAAGISKNGQT*EHALLAY

Parental  TLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWF
          TL.VKQLIV.VNKMDSTEPPYSQKRYEE.VKEVSTYIKK.GYNP.T.AFVPISGWNGDNM.EP.ANMPWF
Retrocopy TLEVKQLIVSVNKMDSTEPPYSQKRYEEAVKEVSTYIKKTGYNPNTAAFVPISGWNGDNMWEPNANMPWF

Parental  KGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYK-IGGIGTVPVGRVETGVLKPGMVVTF
          KGWK.TRKDGN.SGTTLL.ALDCILPPTRPT.KPL.LPLQDVY...GGIGTVPVG.V.TGVLKPGMVVTF
Retrocopy KGWKTTRKDGNTSGTTLLGALDCILPPTRPTNKPLCLPLQDVYR>SGGIGTVPVG*VQTGVLKPGMVVTF

Parental  APVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHP
          APVNVTTEVKSVEMHHEAL..ALPGDNVGFNVKNVSVKDV...N.AGDSKNDPPMEAAGFTAQ.IILNHP
Retrocopy APVNVTTEVKSVEMHHEALRKALPGDNVGFNVKNVSVKDVHHSNDAGDSKNDPPMEAAGFTAQGIILNHP

Parental  GQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPP
          GQISAGYAPVLDCHTAHIACKFAELKEK.D..SG.KLEDGP.FL.SGDAAIVDMVPGKPM.VE.FSDYP.
Retrocopy GQISAGYAPVLDCHTAHIACKFAELKEKTDHLSGEKLEDGPNFLNSGDAAIVDMVPGKPMYVECFSDYPL

Parental  LGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQK
          LG.FAVR.MRQTV.V.VIKAV.KKAAGAGKVTKSAQKAQK
Retrocopy LGHFAVRGMRQTVVVDVIKAVNKKAAGAGKVTKSAQKAQK

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	257.72 RPM
SRP007412_brain_prefrontal_cortex	0.00 RPM	933.00 RPM
SRP007412_cerebellum	0.19 RPM	305.24 RPM
SRP007412_heart	0.18 RPM	637.02 RPM
SRP007412_kidney	0.82 RPM	964.42 RPM
SRP007412_liver	0.40 RPM	1738.88 RPM
SRP007412_testis	0.19 RPM	580.33 RPM

ChIP-Seq

Macaca mulatta was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_mmul_848 retrocopy.

TSS

Macaca mulatta was not studied using FANTOM5 data.

Lab

retro_mmul_848 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmul_848 has 2 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_1093
Gorilla gorilla	retro_ggor_860

---

Parental genes homology:

Parental genes homology involve 13 parental genes, and 68 retrocopies.

Species	Parental gene accession	Retrocopies number
Canis familiaris	ENSCAFG00000013249	1 retrocopy	retro_cfam_1007,
Callithrix jacchus	ENSCJAG00000010051	6 retrocopies	retro_cjac_1431, retro_cjac_3075, retro_cjac_34, retro_cjac_3735, retro_cjac_733, retro_cjac_97,
Cavia porcellus	ENSCPOG00000004067	15 retrocopies	retro_cpor_1150, retro_cpor_1162, retro_cpor_1191, retro_cpor_1256, retro_cpor_1393, retro_cpor_1492, retro_cpor_1499, retro_cpor_348, retro_cpor_381, retro_cpor_387, retro_cpor_413, retro_cpor_709, retro_cpor_891, retro_cpor_918, retro_cpor_959,
Dipodomys ordii	ENSDORG00000009629	1 retrocopy	retro_dord_509,
Equus caballus	ENSECAG00000022260	1 retrocopy	retro_ecab_802,
Erinaceus europaeus	ENSEEUG00000000342	3 retrocopies	retro_eeur_407, retro_eeur_492, retro_eeur_625,
Loxodonta africana	ENSLAFG00000028081	1 retrocopy	retro_lafr_651,
Macaca mulatta	ENSMMUG00000004852	15 retrocopies	retro_mmul_1375, retro_mmul_1407, retro_mmul_1518, retro_mmul_1525, retro_mmul_1690, retro_mmul_1707, retro_mmul_1721, retro_mmul_1739, retro_mmul_1743, retro_mmul_1906, retro_mmul_2196, retro_mmul_2250, retro_mmul_323, retro_mmul_6, retro_mmul_848 ,
Monodelphis domestica	ENSMODG00000018533	4 retrocopies	retro_mdom_1053, retro_mdom_1146, retro_mdom_311, retro_mdom_792,
Mus musculus	ENSMUSG00000037742	15 retrocopies	retro_mmus_1349, retro_mmus_1518, retro_mmus_1667, retro_mmus_1783, retro_mmus_1816, retro_mmus_1849, retro_mmus_1850, retro_mmus_1864, retro_mmus_1909, retro_mmus_2363, retro_mmus_2583, retro_mmus_2902, retro_mmus_3249, retro_mmus_480, retro_mmus_483,
Pan troglodytes	ENSPTRG00000018347	1 retrocopy	retro_ptro_71,
Ictidomys tridecemlineatus	ENSSTOG00000007687	4 retrocopies	retro_itri_1509, retro_itri_1740, retro_itri_1816, retro_itri_195,
Drosophila melanogaster	FBgn0000557	1 retrocopy	retro_dmel_9,