RetrogeneDB ID: retro_ecab_504

Retrocopy location

• Organism: Horse (Equus caballus)
• Coordinates: 2:103287161..103287770(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: AKTIP
• Ensembl ID: ENSECAG00000020381
• Aliases: None
• Description: AKT interacting protein [Source:HGNC Symbol;Acc:16710]

Sequence

>retro_ecab_504
CAGTAAACAAATGGCACACATGCTTTTTACAGACCCTTCCATCCGGAACACTCCCTTCTTGCGGTATTTACCTTGCTTGT
GAAGCAGAAGTTCTCTGGTGTCTATGTGCAGCCATCTTATAGCTCTTCATTAATGTAGGTCAGAGCAACATTCATATGCG
ATGGACTTTATCAAAGTGGCATATTTACATTTACAGTGTATATCACTGATAACTATCCAGATGGTGACTGTCCATGATGA
GGTTTGATCTGATGAGGTCTGTCTTTCACCCACTAGTTGATTCCAACGCAGGTCAACTAGAGATGCAGAGAGCATTTGCA
AAATGCAGGTGGAGCCATAAACATAGTTGTTAAGTATTTATGTATCCATGGGGAGTTGAAACAGCAAGACTCCTGAACCC
AGAGTCTGCAGTACTGTATGAAAAGATAGTCAACTTTTTAAAGTAAAATGCTTGACAGTATTGACATGCACTGCTTGCTT
GTTTGATAAATCTAAATTGGAAGACACTATGCAATTAGTTTTTCTCTGTGGAATTCTTCTGTATGTGATGAACTCATAGA
GATGATGCTATCTTATAGAAAGAAGACTGATGAACACCATAATAAAACA

Alignment

ORF - retro_ecab_504 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 59.43 %
• Parental protein coverage: 77.53 %
• Number of stop codons detected: 3
• Number of frameshifts detected: 3

Retrocopy - Parental Gene Alignment:

Parental  QSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPD
          Q.TNGTHA.Y.PF..E.SLLA.FTL.VKQK..GVYVQPSY.S.LM.....FI..GLYQ.G.F.FTVYI.D
Retrocopy Q*TNGTHAFYRPFHPEHSLLAVFTLLVKQKFSGVYVQPSYSSSLM*VRATFICDGLYQSGIFTFTVYITD

Parental  NYPDGDCP-RLVFDI--PVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPE
          NYPDGDCP....FD....VFHPLVD...G.L...RAFAK.R..H.H...V.MY.........TA..LNPE
Retrocopy NYPDGDCP<MMRFDLMRSVFHPLVDSNAGQLEMQRAFAKCRWSHKHSC*VFMYP----WGVETARLLNPE

Parental  AAVLYE-KDIQLFKSKVIDSVKACTARLFDQPKIEDP-YAISFSPWNPSVHDEAREKMLTQKKKPEEQHN
          .AVLYE.KD.QLFK.K....V..CTA.LFD..K.ED..YAISFS.WN.SV.DE..E.ML...KK..E.HN
Retrocopy SAVLYE<KDSQLFKVKCL-TVLTCTACLFDKSKLEDT<YAISFSLWNSSVCDELIEMMLSYRKKTDEHHN

Parental  KS
          K.
Retrocopy KT

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP021940_articular_cartilage	0.00 RPM	4.72 RPM
SRP021940_cerebellum	0.00 RPM	15.01 RPM
SRP021940_embryo	0.00 RPM	11.58 RPM
SRP021940_placental_villous	0.00 RPM	19.63 RPM
SRP021940_synovial_membrane	0.00 RPM	6.73 RPM
SRP021940_testis	0.00 RPM	40.35 RPM

ChIP-Seq

Equus caballus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ecab_504 retrocopy.

TSS

Equus caballus was not studied using FANTOM5 data.

Lab

retro_ecab_504 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ecab_504 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 20 parental genes, and 32 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000016345	1 retrocopy	retro_amel_392,
Canis familiaris	ENSCAFG00000009500	1 retrocopy	retro_cfam_1826,
Choloepus hoffmanni	ENSCHOG00000013690	2 retrocopies	retro_chof_2533, retro_chof_629,
Callithrix jacchus	ENSCJAG00000001498	1 retrocopy	retro_cjac_2895,
Cavia porcellus	ENSCPOG00000006523	1 retrocopy	retro_cpor_622,
Dasypus novemcinctus	ENSDNOG00000004906	4 retrocopies	retro_dnov_1376, retro_dnov_1776, retro_dnov_2080, retro_dnov_756,
Equus caballus	ENSECAG00000020381	1 retrocopy	retro_ecab_504 ,
Homo sapiens	ENSG00000166971	2 retrocopies	retro_hsap_1458, retro_hsap_3152,
Gorilla gorilla	ENSGGOG00000014821	1 retrocopy	retro_ggor_1122,
Microcebus murinus	ENSMICG00000010810	3 retrocopies	retro_mmur_124, retro_mmur_333, retro_mmur_985,
Microcebus murinus	ENSMICG00000016793	1 retrocopy	retro_mmur_1191,
Monodelphis domestica	ENSMODG00000025788	1 retrocopy	retro_mdom_1881,
Nomascus leucogenys	ENSNLEG00000001106	1 retrocopy	retro_nleu_2660,
Otolemur garnettii	ENSOGAG00000014495	1 retrocopy	retro_ogar_365,
Oryzias latipes	ENSORLG00000013747	1 retrocopy	retro_olat_30,
Pongo abelii	ENSPPYG00000007351	2 retrocopies	retro_pabe_1204, retro_pabe_1205,
Pan troglodytes	ENSPTRG00000008116	1 retrocopy	retro_ptro_2282,
Rattus norvegicus	ENSRNOG00000011956	1 retrocopy	retro_rnor_1611,
Tupaia belangeri	ENSTBEG00000017105	5 retrocopies	retro_tbel_1063, retro_tbel_2864, retro_tbel_3350, retro_tbel_4130, retro_tbel_853,
Tursiops truncatus	ENSTTRG00000012705	1 retrocopy	retro_ttru_348,