RetrogeneDB ID: retro_dnov_441

Retrocopy location

• Organism: Armadillo (Dasypus novemcinctus)
• Coordinates: GeneScaffold_4741:272535..273065(+)
• Located in intron of: ENSDNOG00000009562

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: APOPT1
• Ensembl ID: ENSDNOG00000019553
• Aliases: None
• Description: apoptogenic 1, mitochondrial [Source:HGNC Symbol;Acc:20492]

Sequence

>retro_dnov_441
AGGACTCGCCTCCCTCTTCTGTGCCCCACTTTCACCAGCCCAGGCAGACAGCTGACCCCAGAGCACAGCGCCCAGCTCAG
GGATGCCAGGCTGAGCCAGTCTCTAGTTCCCGTCACAATTGGATAGGACCCCAGATAAATAGTCAAACCTTTGATCTATT
CAGTTTTACAACCCTGAAAATGAGTCACCATTGGAACAAAAGCTGAGAGAATTGAGACAAGAATCACAATAAAGATATCA
ACAATTTGGGCAAACCAGAATTTGACTTTTAGTAACAAAAAAGAAGAATTCATTCACTTGAGACTGAACTCCAAAGGCCT
GGGAGTGAGGGCTGGCTCAGTTCAAAAAGCAACACTGAAAGCAGAGGAAATGGCTGAGTTTTACAAGGAATTTTTAAATA
AAATTTCCTGAAGCATATGTATTATAACAGAGACTAGTATAAGTGTAACTTTACCATCACATTCTTCATGGGAAAAGTGG
CCCTGGAGAGAATTTACAAAAAGTTTGGACTGAAACAAAACAAGACCACC

Alignment

ORF - retro_dnov_441 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 66.48 %
• Parental protein coverage: 95.19 %
• Number of stop codons detected: 4
• Number of frameshifts detected: 4

Retrocopy - Parental Gene Alignment:

Parental  RLLLPLLGRAFAGRGRQPAPERGAERREAAPSR-VSSSHHDWIGP-PDKYSNLRPVQFYIPENESPLERK
          R..LPLL...F...GRQ..PE..A..R.A..S..VSSS.H.WIGP.PDK.SNL...QFY.PENESPLE.K
Retrocopy RTRLPLLCPTFTSPGRQLTPEHSAQLRDARLSQ<VSSSRHNWIGP<PDK*SNL*SIQFYNPENESPLEQK

Parental  LRELRQETQEWNQRF-WTNQNLTFSKEKEEFIHSRLKARGLGGSAGSGQKPTLNAEEMAEFYKEFLNK-N
          LRELRQE.Q...Q.F.W.NQNLTFS..KEEFIH.RL...GLG..AGS.QK.TL.AEEMAEFYKEFLNK.N
Retrocopy LRELRQESQ*RYQQF<WANQNLTFSNKKEEFIHLRLNSKGLGVRAGSVQKATLKAEEMAEFYKEFLNK<N

Parental  FQKHMYYNRGWYKHNFAVTFFLGKLTPKRIWKKFGLKQKKTS
          F.KHMYYNR..YK.NF..TFF.GK....RI.KKFGLKQ.KT.
Retrocopy FLKHMYYNRD*YKCNFTITFFMGKVALERIYKKFGLKQNKTT

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012922_ascending_colon	0.00 RPM	6.61 RPM
SRP012922_cerebellum	0.14 RPM	8.39 RPM
SRP012922_heart	0.00 RPM	4.64 RPM
SRP012922_kidney	0.00 RPM	4.38 RPM
SRP012922_liver	0.00 RPM	2.01 RPM
SRP012922_lung	0.00 RPM	3.82 RPM
SRP012922_quadricep_muscle	0.00 RPM	8.48 RPM
SRP012922_spleen	0.00 RPM	4.24 RPM

ChIP-Seq

Dasypus novemcinctus was not studied using ChIP-Seq data.

EST

Dasypus novemcinctus was not studied using EST data.

TSS

Dasypus novemcinctus was not studied using FANTOM5 data.

Lab

retro_dnov_441 was not experimentally validated.

Homology

Retrocopy orthology:

Dasypus novemcinctus does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 7 parental genes, and 15 retrocopies.

Species	Parental gene accession	Retrocopies number
Canis familiaris	ENSCAFG00000030884	2 retrocopies	retro_cfam_1514, retro_cfam_396,
Dasypus novemcinctus	ENSDNOG00000019553	5 retrocopies	retro_dnov_1003, retro_dnov_2304, retro_dnov_2391, retro_dnov_441 , retro_dnov_777,
Erinaceus europaeus	ENSEEUG00000011728	1 retrocopy	retro_eeur_107,
Felis catus	ENSFCAG00000027939	3 retrocopies	retro_fcat_482, retro_fcat_549, retro_fcat_649,
Microcebus murinus	ENSMICG00000009792	1 retrocopy	retro_mmur_744,
Mustela putorius furo	ENSMPUG00000002350	2 retrocopies	retro_mputfur_1406, retro_mputfur_166,
Otolemur garnettii	ENSOGAG00000015416	1 retrocopy	retro_ogar_1394,