RetrogeneDB ID: retro_dnov_2426

Retrocopy location

• Organism: Armadillo (Dasypus novemcinctus)
• Coordinates: scaffold_73993:8263..9064(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: GHITM
• Ensembl ID: ENSDNOG00000018880
• Aliases: None
• Description: growth hormone inducible transmembrane protein [Source:HGNC Symbol;Acc:17281]

Sequence

>retro_dnov_2426
GATCAGATGGAAGATGGTTTGTTGCTGGAGGAGCTGCTGTTGGTCTTAGAGCATTGTGCTACTGTGGCTTGGGACTGTCT
AATGAAATTGGAAATATTGAAAAGGCTACAATTTGGCCTCAATACTTAAAGGACGAATTCAATCTACCTGCATGTACTTA
GCAGGAAGTATTGGTTTAACAGCTTTGTCTACCTTGACAATGATCAGATCTCATGTTCTCATGAGCTTTATGATGAGAGG
CTCTTGGGTGATGATTGGTGCCACCATTGCAGGCTCGATTGGAGCTGGCATGCTGGTACAATCCTTGTCATATGATCGGA
GTCCCAGTCCAAAGCATCTGACTGACTGCTACATTCTGGTGTCATGGTTGCAGTGATAGCTCCCCTGGAGATCTTAAGGG
GCCCTCATCTCATTAAAGCTGTGTGGTACACAGCTGGCATTGTGGGTGGCCTCTCCACCAAGGTTATGTGTCTCCCAGGA
AAGTTCATAAATATGGGCGCACCCTGGGGAGTGGGCCTGGGTTTCGTCTTTGTGCCCTCACTGGGATCTATTTTTCTTCC
AACTACCTCAGTGGCTAGGCACCACTCTATACTCAGTGGCAGTTTACAGTGGATTGGTTCTTTTCAGCATGTTTCTCTAG
TACGACACTCAGAAAGTAATCAGGCAGGCCGAAGTAGCACTGTTATATGGAGGTCAATAATACAATCTCATTAATTAGAT
GATGGGAATCTACGTGGATACATTAAATATATTTATGTGAGTTGTCACTGTGCTGGGCAACTGGGAAGCAACAGAAAGAA
A

Alignment

ORF - retro_dnov_2426 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 74.09 %
• Parental protein coverage: 77.71 %
• Number of stop codons detected: 5
• Number of frameshifts detected: 6

Retrocopy - Parental Gene Alignment:

Parental  DQM-GRWFVAGGAAVGLGALCYYGLGMSNEIGAIEKAVIWPQYVKD-RIHSTYMYLAGSIGLTALSALAM
          DQM.GRWFVAGGAAVGL.ALCY.GLG.SNEIG.IEKA.IWPQY.KD.RI.ST.MYLAGSIGLTALS.L.M
Retrocopy DQM<GRWFVAGGAAVGLRALCYCGLGLSNEIGNIEKATIWPQYLKD<RIQSTCMYLAGSIGLTALSTLTM

Parental  TRSPALMGFMMRGSWVTIGATFAAMIGAGMLVRSIPYDQSPGPKHLA-WLLHSGVMGAVVAPLAILGGPL
          .RS..LM.FMMRGSWV.IGAT.A..IGAGMLV.S..YD.SP.PKHL...LLHSGVM.AV.APL.IL.GP.
Retrocopy IRSHVLMSFMMRGSWVMIGATIAGSIGAGMLVQSLSYDRSPSPKHLT<*LLHSGVMVAVIAPLEILRGPH

Parental  LIRAAWYTAGIVGGLSTVAMCAPSEKFMNMGAPLGVGLGLVFVSSLGSMFLPPTTVA-GATLYSVAVYGG
          LI.A.WYTAGIVGGLST..MC.P..KF.NMGAP.GVGLG.VFV.SLGS.FLP.T.VA.G.TLYSVAVY.G
Retrocopy LIKAVWYTAGIVGGLSTKVMCLPG-KFINMGAPWGVGLGFVFVPSLGSIFLPTTSVA>GTTLYSVAVYSG

Parental  LVLFSMFLLYDTQKVIWRAEGAPLYGVQKYDPIN--TGIYIDALNLFM-VAAML-ATG-SNRKK
          LVLFSMFL.YDTQKVI..AE.A.LYG.Q.Y..IN...GIY.D.LN.FM.V...L.ATG.SNRKK
Retrocopy LVLFSMFL*YDTQKVIRQAEVALLYGGQ*YNLIN*MMGIYVDTLNIFM*VVTVL>ATG>SNRKK

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012922_ascending_colon	0.00 RPM	148.18 RPM
SRP012922_cerebellum	0.00 RPM	145.30 RPM
SRP012922_heart	0.00 RPM	71.23 RPM
SRP012922_kidney	0.00 RPM	130.87 RPM
SRP012922_liver	0.00 RPM	73.38 RPM
SRP012922_lung	0.00 RPM	61.85 RPM
SRP012922_quadricep_muscle	0.00 RPM	97.79 RPM
SRP012922_spleen	0.00 RPM	59.86 RPM

ChIP-Seq

Dasypus novemcinctus was not studied using ChIP-Seq data.

EST

Dasypus novemcinctus was not studied using EST data.

TSS

Dasypus novemcinctus was not studied using FANTOM5 data.

Lab

retro_dnov_2426 was not experimentally validated.

Homology

Retrocopy orthology:

Dasypus novemcinctus does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 18 parental genes, and 28 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000009753	1 retrocopy	retro_amel_336,
Bos taurus	ENSBTAG00000032829	2 retrocopies	retro_btau_1689, retro_btau_779,
Choloepus hoffmanni	ENSCHOG00000001576	1 retrocopy	retro_chof_5,
Cavia porcellus	ENSCPOG00000006432	1 retrocopy	retro_cpor_1436,
Dasypus novemcinctus	ENSDNOG00000018880	5 retrocopies	retro_dnov_2426 , retro_dnov_2514, retro_dnov_2524, retro_dnov_708, retro_dnov_812,
Dipodomys ordii	ENSDORG00000001109	1 retrocopy	retro_dord_184,
Echinops telfairi	ENSETEG00000001796	1 retrocopy	retro_etel_68,
Loxodonta africana	ENSLAFG00000009646	1 retrocopy	retro_lafr_388,
Macropus eugenii	ENSMEUG00000009861	3 retrocopies	retro_meug_1431, retro_meug_1806, retro_meug_798,
Microcebus murinus	ENSMICG00000011621	1 retrocopy	retro_mmur_692,
Monodelphis domestica	ENSMODG00000001693	3 retrocopies	retro_mdom_1008, retro_mdom_407, retro_mdom_886,
Oryctolagus cuniculus	ENSOCUG00000006093	1 retrocopy	retro_ocun_260,
Otolemur garnettii	ENSOGAG00000012120	1 retrocopy	retro_ogar_1287,
Rattus norvegicus	ENSRNOG00000013961	1 retrocopy	retro_rnor_2461,
Sus scrofa	ENSSSCG00000010345	1 retrocopy	retro_sscr_1102,
Tupaia belangeri	ENSTBEG00000016365	2 retrocopies	retro_tbel_1601, retro_tbel_2819,
Tarsius syrichta	ENSTSYG00000006367	1 retrocopy	retro_tsyr_4,
Drosophila melanogaster	FBgn0030263	1 retrocopy	retro_dmel_61,