RetrogeneDB ID: retro_dnov_154

Retrocopy location

• Organism: Armadillo (Dasypus novemcinctus)
• Coordinates: GeneScaffold_2159:57354..57852(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: ENSDNOG00000025364
• Aliases: None
• Status: KNOWN_PSEUDOGENE

Parental gene information

• Parental gene symbol: HRAS
• Ensembl ID: ENSDNOG00000015293
• Aliases: None
• Description: Harvey rat sarcoma viral oncogene homolog [Source:HGNC Symbol;Acc:5173]

Sequence

>retro_dnov_154
CTAATACAGAACCACTTTGTAGATGAGTATGATCCCACCATAGAGGATTCTTACCGAAAAAAGGTGGTTATAGATGGTGA
AACCTGTCTGCTGGACATTCTGGATACAGCCGGACAAGAGGAGTACAGTGCCATGAGAGACCAGTACATGAGGACAGGCG
AAGGTTTCCTCTGTGTGTTTGCCATCAATAATAGCAAATCATTTGCAGATATTAACCTCTACAGAGAGCAGATTAAACGA
GTTAAAGATTCAGATGATGTACCTATGGTGCTGGTAGGAAACAGGTGTGATTTGCCAACAAGGACAGTTGACACAAAACA
AGCCCATGAACTGGCCAAGAGTTATGGGATTCCATTCATTGAAACCTCAGCCAAGACCAGACAGGGTGTTGAAGAGGCCT
TTTACACACTGGTAAGAGAAATACATCAGTATCGAATGAAAAAACTCAACAGCAATGATGATGGGACTCAAGGTTGTATG
GGGTTGCCGTGTGTGGTG

Alignment

ORF - retro_dnov_154 Open Reading Frame is conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 80.72 %
• Parental protein coverage: 87.83 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 0

Retrocopy - Parental Gene Alignment:

Parental  LIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFED
          LIQNHFVDEYDPTIEDSYRK.VVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINN.KSF.D
Retrocopy LIQNHFVDEYDPTIEDSYRKKVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFAD

Parental  IHQYREQIKRVKDSDDVPMVLVGNKCDLPARTVDARQAQELACSYGLPYVETSAKTRQGVEDAFYTLVRE
          I..YREQIKRVKDSDDVPMVLVGN.CDLP.RTVD..QA.ELA.SYG.P..ETSAKTRQGVE.AFYTLVRE
Retrocopy INLYREQIKRVKDSDDVPMVLVGNRCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEEAFYTLVRE

Parental  IRQHKLRKLNPPDESGPGCADCKCTL
          I.Q....KLN..D....GC....C..
Retrocopy IHQYRMKKLNSNDDGTQGCMGLPCVV

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012922_ascending_colon	23.53 RPM	11.67 RPM
SRP012922_cerebellum	4.12 RPM	17.73 RPM
SRP012922_heart	1.62 RPM	7.19 RPM
SRP012922_kidney	7.67 RPM	23.00 RPM
SRP012922_liver	6.66 RPM	8.20 RPM
SRP012922_lung	9.62 RPM	17.72 RPM
SRP012922_quadricep_muscle	2.77 RPM	37.73 RPM
SRP012922_spleen	12.82 RPM	9.39 RPM

ChIP-Seq

Dasypus novemcinctus was not studied using ChIP-Seq data.

EST

Dasypus novemcinctus was not studied using EST data.

TSS

Dasypus novemcinctus was not studied using FANTOM5 data.

Lab

retro_dnov_154 was not experimentally validated.

Homology

Retrocopy orthology:

Dasypus novemcinctus does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

---

Parental genes homology:

Parental genes homology involve 8 parental genes, and 32 retrocopies.

Species	Parental gene accession	Retrocopies number
Dasypus novemcinctus	ENSDNOG00000007809	5 retrocopies	retro_dnov_101, retro_dnov_1617, retro_dnov_1764, retro_dnov_628, retro_dnov_94,
Dasypus novemcinctus	ENSDNOG00000015293	3 retrocopies	retro_dnov_1358, retro_dnov_154 , retro_dnov_2354,
Dasypus novemcinctus	ENSDNOG00000017413	1 retrocopy	retro_dnov_573,
Dasypus novemcinctus	ENSDNOG00000019724	18 retrocopies	retro_dnov_1243, retro_dnov_1249, retro_dnov_1323, retro_dnov_1395, retro_dnov_1574, retro_dnov_1710, retro_dnov_1763, retro_dnov_1841, retro_dnov_186, retro_dnov_1976, retro_dnov_2033, retro_dnov_217, retro_dnov_2679, retro_dnov_298, retro_dnov_318, retro_dnov_719, retro_dnov_761, retro_dnov_84,
Echinops telfairi	ENSETEG00000019899	2 retrocopies	retro_etel_1028, retro_etel_391,
Macropus eugenii	ENSMEUG00000008467	1 retrocopy	retro_meug_852,
Microcebus murinus	ENSMICG00000006472	1 retrocopy	retro_mmur_948,
Rattus norvegicus	ENSRNOG00000016611	1 retrocopy	retro_rnor_2941,