RetrogeneDB ID: retro_pabe_1333

Retrocopy location

• Organism: Orangutan (Pongo abelii)
• Coordinates: 15_random:6066098..6067216(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: TMEM183A
• Ensembl ID: ENSPPYG00000000340
• Aliases: None
• Description: transmembrane protein 183A [Source:HGNC Symbol;Acc:20173]

Sequence

>retro_pabe_1333
ATGGCCCAGGGGCCCGGCACGCTAGGCAGGCCTCACCCAGATACAATCGCCCTGCCCAAGAGAGGAAAGCGACTCAAGTC
GCTCGTCTGCTCCGGCGGAGTGACCATGAAGGATTACGCCAACTCAGATCTGGTGGTCGTGAGGTCTGGACGAGTCAAGA
AAGCCATAGCCAACGCTGTTCAGCAGGAAGTAAAATGTTTTTGAGGCTTGGAAGCCTCTCAGGTTCCTGCAGAGGAAGTT
CGTTCTGGGGCTGGTAAGCCCATGACATCATCGACAGCAGTGATGAGATGGATGCCCAGGACGAAAGCATCCATGAGAGA
ACTGTCTCCAGAAAAACGAAAAGCAAGAGGCACAAAGAAGAACTGGACGGGGCTGGAGGAAAAGAGTATCCCATGGATTT
TTGGCTATTGCTGGCCTCCTATATTTGTTCTGAGAACATTGTGAATTTTTTCCTGATTTCTAAGAATGCCTGGACTGTCA
TTTGTACTGCTGCCTTTTGGACCAGGTTGTACCAAAGGCACTACACGCTGGATGCTTCTCTGCCTTTGCGTCTGCGACCA
GAGTCAATGGAGAAGCTGCGCTGTCTCTGGGCTTGTGTGATCCGATCTCTGTACCATATGTATGAGCCATTTACTGCTGG
AATCTCCAAGAATCCAGCCATTCCAGAAAGCACCCCCAGCACATTAAAGAATTCCAAATGCTTACTTTTCTGGTGCAGAA
AGATTGTTGGGAACAGACGGGAACCAATGTGGGAATTCAACTTCACGTTCAAAAAACAGTCCGCTAGGTTAAAGAGCAAG
TGTACAGGAGGACTGCAGCTTCCCATTCAGTACGAAGATGTTCATACCAATCGAGACCAGGACTGCTGCCTACTGCAGGT
CACCACCCTCAATTTCATCTTTATTCCGATTGTCATGGGAATAATATTTACTCTGTTTACTGTCAATGCAAGCACGGACA
CGCGGCATCACCGAGTGAGACTGGTGTTCCAAGATTCCCCTGTCCATGGTGGTCAGAAACTGCGCAGTGAACAGGGTGTG
CAAGTCATGCTGGACCCAGTGCACGGCGTTCGGCTCTTTGACTGGTGGCTTTCTCAGTACCCATTCTCCCTGACAGCA

Alignment

ORF - retro_pabe_1333 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 83.82 %
• Parental protein coverage: 100.0 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  MARGPGPLGRPRPDTVAMPKRGKRLKFRAHDACSGRVTVADYANSDPAVVRSGRVKKAVANAVQQEVKSL
          MA.GPG.LGRP.PDT.A.P...KR.K......CSG.VT..DYANSD..VVRSGRVKKA.ANAVQQEVK..
Retrocopy MAQGPGTLGRPHPDTIALP---KRGKRLKSLVCSGGVTMKDYANSDLVVVRSGRVKKAIANAVQQEVKCF

Parental  CGLEASQVPAEEALSGAGEPC-DIIDSSDEMEAQEESIHERTVSRKKKSKRHKEELDGAG-EEYPMDIWL
          .GLEASQVPAEE..SGAG.P..DIIDSSDEM.AQ.ESIHERTVSRK.KSKRHKEELDGAG..EYPMD.WL
Retrocopy *GLEASQVPAEEVRSGAGKPM<DIIDSSDEMDAQDESIHERTVSRKTKSKRHKEELDGAGGKEYPMDFWL

Parental  LLASYIRPEDIVNFSLICKNAWTVTCTAAFWTRLYRRHYTLDASLPLRLRPESMEKLRCLRACVIRSLYH
          LLASYI..E.IVNF.LI.KNAWTV.CTAAFWTRLY.RHYTLDASLPLRLRPESMEKLRCL.ACVIRSLYH
Retrocopy LLASYICSENIVNFFLISKNAWTVICTAAFWTRLYQRHYTLDASLPLRLRPESMEKLRCLWACVIRSLYH

Parental  MYEPFAARISKNPAIPESTPSTLKNSKCLLFWCRKIVGNRQEPMWEFNFKFKKQSPRLKSKCTGGLQPPV
          MYEPF.A.ISKNPAIPESTPSTLKNSKCLLFWCRKIVGNR.EPMWEFNF.FKKQS.RLKSKCTGGLQ.P.
Retrocopy MYEPFTAGISKNPAIPESTPSTLKNSKCLLFWCRKIVGNRREPMWEFNFTFKKQSARLKSKCTGGLQLPI

Parental  QYEDVHTNPDQDCCLLQVTTLNFIFIPIVMGMIFTLFTINVSTDMRHHRVRLVFQDSPVHGGRKLRSEQG
          QYEDVHTN.DQDCCLLQVTTLNFIFIPIVMG.IFTLFT.N.STD.RHHRVRLVFQDSPVHGG.KLRSEQG
Retrocopy QYEDVHTNRDQDCCLLQVTTLNFIFIPIVMGIIFTLFTVNASTDTRHHRVRLVFQDSPVHGGQKLRSEQG

Parental  VQVILDPVHSVRLFDWWHPQYPFSLRA
          VQV.LDPVH.VRLFDWW..QYPFSL.A
Retrocopy VQVMLDPVHGVRLFDWWLSQYPFSLTA

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain_prefrontal_cortex	0.11 RPM	96.92 RPM
SRP007412_cerebellum	0.00 RPM	117.10 RPM
SRP007412_heart	0.00 RPM	13.31 RPM
SRP007412_kidney	0.00 RPM	44.25 RPM
SRP007412_liver	0.00 RPM	43.36 RPM

ChIP-Seq

Pongo abelii was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_pabe_1333 retrocopy.

TSS

Pongo abelii was not studied using FANTOM5 data.

Lab

retro_pabe_1333 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_pabe_1333 has 0 orthologous retrocopies within eutheria group .

---

Parental genes homology:

Parental genes homology involve 14 parental genes, and 21 retrocopies.

Species	Parental gene accession	Retrocopies number
Echinops telfairi	ENSETEG00000008318	1 retrocopy	retro_etel_1835,
Felis catus	ENSFCAG00000013106	1 retrocopy	retro_fcat_3,
Homo sapiens	ENSG00000163444	3 retrocopies	retro_hsap_2808, retro_hsap_2863, retro_hsap_3157,
Gorilla gorilla	ENSGGOG00000009834	2 retrocopies	retro_ggor_1358, retro_ggor_2533,
Loxodonta africana	ENSLAFG00000018443	1 retrocopy	retro_lafr_149,
Macaca mulatta	ENSMMUG00000000801	1 retrocopy	retro_mmul_1532,
Nomascus leucogenys	ENSNLEG00000018106	2 retrocopies	retro_nleu_2541, retro_nleu_2663,
Otolemur garnettii	ENSOGAG00000000911	1 retrocopy	retro_ogar_3209,
Pongo abelii	ENSPPYG00000000340	2 retrocopies	retro_pabe_1333 , retro_pabe_2627,
Pan troglodytes	ENSPTRG00000001864	2 retrocopies	retro_ptro_1902, retro_ptro_2278,
Rattus norvegicus	ENSRNOG00000003594	2 retrocopies	retro_rnor_1954, retro_rnor_2386,
Sus scrofa	ENSSSCG00000015473	1 retrocopy	retro_sscr_879,
Ictidomys tridecemlineatus	ENSSTOG00000012466	1 retrocopy	retro_itri_421,
Tarsius syrichta	ENSTSYG00000007009	1 retrocopy	retro_tsyr_717,