RetrogeneDB ID: retro_ocun_475

Retrocopy location

• Organism: Rabbit (Oryctolagus cuniculus)
• Coordinates: 11:48219843..48220618(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: EIF4A1
• Ensembl ID: ENSOCUG00000010375
• Aliases: None
• Description: Eukaryotic initiation factor 4A-I [Source:UniProtKB/Swiss-Prot;Acc:P29562]

Sequence

>retro_ocun_475
GAGTCCCTCCTTCATGGCATCTATGTCCGTGGTTTTGAGAAGCCTTCTGCCATCCAGCAGTGTGCCAATCTTCCTCGTAT
CAAGGATTATGATGTGATCATTCAAGTCCAATCTGGAAGTGGAAAAAATGGCCACACTGGCCATATTAATTCTACTGCAG
ATTGAATTGGATCTGAAGGCCACTCAGGCCTTAGTACTAGTAACTACTAGGAAATTGACTCAACAGGTACAAAAGGTGTT
TATGGCATTAGGAGATTATATGCGTGACTCCTGTCTTGCCTGTTTTGCAGACCCCAACTTGCATGCTGTGGTACAGAAAC
TGCAAATGGAAGCTCCCCATGTCATCATGGGCACCACTCTCCACTTGTTTCATATGCTTAACCAGAGATAATTGTCTCCC
AAATACTTGAAGACATTTGTACTGGACAAAACTGATGAAACATTAGGGCATGGATTCAAGGACCAGGTCTATGATATATT
CTAAAAGCTGAACAGCAACACCTAGGTGGTTTTGGTGTGAGCCATAATGCCTTCCAATGTGCCTGAGGTGACCACGGAGT
TCATGAAGGACCCCATCTGGATCCTTGCCAAGAAGGAAGAGTTGTCCCTGGAAGGTATCTGCCATCTCTACATCAATATA
GAATGAGAAGAATAGAAGCTGGACACACTATGTGACTTGTATGAAACCTTGACCATAACCCAGGCTGTCAATTTCATCAA
TACCAGAAGGAAGGTTGATTGGCTCAGTGCGATGCATGAACAAGACTTCATTGTC

Alignment

ORF - retro_ocun_475 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 71.54 %
• Parental protein coverage: 63.79 %
• Number of stop codons detected: 6
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  ESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKT-ATFAISILQQIELDLKATQALVLAPT
          ESLL.GIY..GFEKPSAIQQ.A.LP.IK.YDVI.Q.QSG.GK..AT.AI.IL.QIELDLKATQALVL..T
Retrocopy ESLLHGIYVRGFEKPSAIQQCANLPRIKDYDVIIQVQSGSGKN>ATLAILILLQIELDLKATQALVLVTT

Parental  RELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMF
          R.L.QQ.QKV.MALGDYM..SC.AC....N..A.VQKLQMEAPH.I.GT....F.MLN.R.LSPKY.K.F
Retrocopy RKLTQQVQKVFMALGDYMRDSCLACFADPNLHAVVQKLQMEAPHVIMGTTLHLFHMLNQR*LSPKYLKTF

Parental  VLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQF
          VLD..DE.L..GFKDQ.YDIF.KLNSNT.VVL..A.MPS.V.EVT..FM.DPI.IL.KKEEL.LEGI...
Retrocopy VLDKTDETLGHGFKDQVYDIF*KLNSNT*VVLV*AIMPSNVPEVTTEFMKDPIWILAKKEELSLEGICHL

Parental  YINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTV
          YIN.E.EE.KLDTLCDLYETLTITQAV.FINTRRKVDWL...MH..DF.V
Retrocopy YINIE*EE*KLDTLCDLYETLTITQAVNFINTRRKVDWLS-AMHEQDFIV

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP017611_brain	0.00 RPM	85.60 RPM
SRP017611_kidney	0.00 RPM	143.12 RPM
SRP017611_liver	0.00 RPM	73.91 RPM

ChIP-Seq

Oryctolagus cuniculus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ocun_475 retrocopy.

TSS

Oryctolagus cuniculus was not studied using FANTOM5 data.

Lab

retro_ocun_475 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ocun_475 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 7 parental genes, and 22 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000000132	1 retrocopy	retro_btau_1207,
Loxodonta africana	ENSLAFG00000001107	1 retrocopy	retro_lafr_1210,
Macropus eugenii	ENSMEUG00000010780	2 retrocopies	retro_meug_158, retro_meug_179,
Monodelphis domestica	ENSMODG00000007090	3 retrocopies	retro_mdom_1282, retro_mdom_326, retro_mdom_711,
Oryctolagus cuniculus	ENSOCUG00000005011	3 retrocopies	retro_ocun_392, retro_ocun_696, retro_ocun_763,
Oryctolagus cuniculus	ENSOCUG00000010375	5 retrocopies	retro_ocun_1020, retro_ocun_1326, retro_ocun_1375, retro_ocun_475 , retro_ocun_934,
Pongo abelii	ENSPPYG00000007926	7 retrocopies	retro_pabe_1090, retro_pabe_1474, retro_pabe_3759, retro_pabe_3792, retro_pabe_548, retro_pabe_846, retro_pabe_987,