RetrogeneDB ID: retro_cfam_204

Retrocopy location

• Organism: Dog (Canis familiaris)
• Coordinates: 2:60211707..60212115(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: ENSCAFG00000009308
• Aliases: None
• Status: KNOWN_PROTEIN_CODING

Parental gene information

• Parental gene symbol: RPL32
• Ensembl ID: ENSCAFG00000004871
• Aliases: EFCAB12, C20H3orf25
• Description: 60S ribosomal protein L32 [Source:UniProtKB/Swiss-Prot;Acc:E2RKA8]

Sequence

>retro_cfam_204
ATGGCTGCCCTCAGACTTCTGGTGAGGCCCAAGATCGTTAAAAAGAGGACCAAGAAGTTCATCTGGCACCAGTCAGACCG
ATATGTCAAAATTAAGTGCAACTGGCGGAAACCCAGAGGCATTGACAATAGGGTACGCAGAAGATTCAAGGGCCAGATCT
TGATGCCCAACATTGGTTACGGAAGCAACAAGAAAACAAAGCACATGCTGCCCAGTGGCTTCTGGAAGTTCCTAGTCCAC
AACGTCAAGGAGCTTGAAGTGCTGCTGATGTGCAACAAATCTTATTGTGCAAAGATTGCTCACAATGTATCCTCCAAGAA
CCGCAAAGCCATTGTGGAAAGAGCAGCCCAGCTGGCCATCAGAGTCACCATTCCCAATGCCAGGCTGCGTAGTGAAGAAA
ATGAATAG

Alignment

ORF - retro_cfam_204 Open Reading Frame is conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 94.81 %
• Parental protein coverage: 100.0 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 0

Retrocopy - Parental Gene Alignment:

Parental  MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHML
          MAALR.LV.PKIVKKRTKKFI.HQSDRYVKIK.NWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHML
Retrocopy MAALRLLVRPKIVKKRTKKFIWHQSDRYVKIKCNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHML

Parental  PSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE
          PSGF.KFLVHNVKELEVLLMCNKSYCA.IAHNVSSKNRKAIVERAAQLAIRVT.PNARLRSEENE
Retrocopy PSGFWKFLVHNVKELEVLLMCNKSYCAKIAHNVSSKNRKAIVERAAQLAIRVTIPNARLRSEENE

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012049_cerebellum	0.13 RPM	200.54 RPM
SRP017611_brain	0.08 RPM	51.13 RPM
SRP017611_kidney	0.07 RPM	519.25 RPM
SRP017611_liver	0.07 RPM	122.62 RPM

ChIP-Seq

Canis familiaris was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_cfam_204 retrocopy.

TSS

Canis familiaris was not studied using FANTOM5 data.

Lab

retro_cfam_204 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_cfam_204 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 1 parental gene, and 6 retrocopies.

Species	Parental gene accession	Retrocopies number
Canis familiaris	ENSCAFG00000004871	6 retrocopies	retro_cfam_100, retro_cfam_107, retro_cfam_1747, retro_cfam_177, retro_cfam_204 , retro_cfam_234,