RetrogeneDB ID: retro_cfam_1120

Retrocopy location

• Organism: Dog (Canis familiaris)
• Coordinates: 23:15889873..15890541(-)
• Located in intron of: ENSCAFG00000005488

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: FAM60A
• Ensembl ID: ENSCAFG00000010685
• Aliases: None
• Description: family with sequence similarity 60, member A [Source:HGNC Symbol;Acc:30702]

Sequence

>retro_cfam_1120
GAATAAAAGATGTTTAGTTTTACAAACCAAAGATGTGCTGAAGTATAGAGGGCTGCTCTATTTGCAGAGCTAAGTTCTGC
ACTTCTCGGTTTAGTTATAGTAAATAATGAAAGGACTTTCAGAGCTGCAGTTTACAGCTACTCAAGACTTCAGAAAACTT
TTGCAATAGCTGAGTACTATTATGAAAAGATAGAAAAAGCTACCAGCAGGATAAAAAAACAAAACAACTGGAATCATGTT
GTAGGCTCAAGGGCAGGACCCCAGTCTAAACTCTGTATCAAAACCAAAGAAAGTGTAAAGTATCTGGGAAAAAGATAAAG
AAGCAACTAGATTGGTTAACTACAGGAAGAGTTTCAACTTCACGGCCCTGATGCCCACCATACCACCTCAATTACCTCTT
CAACTCAGTCTCCCTGTGCATTTACCAGTCAGATGATGACTTTGATACAGAAATGGCTTCTGGTTCAAACAACACACCTA
CCTTTTCTTTGTAATCTCACGTGTTGGAAAAAGCAGAAACTATGTTGTGGGTTTTAACTATAAAAGGCACTTTGGGGAAG
TCCTTGACACCCACCTATTTAATAAGCCTTGCTTCAGCAGTAAGAAAGCAGTTTCTGAGAAGCCGGAGAAGTGTGGTACT
AGAGCCTCTACCCATTTCCATTGAGGAG

Alignment

ORF - retro_cfam_1120 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 53.88 %
• Parental protein coverage: 97.81 %
• Number of stop codons detected: 12
• Number of frameshifts detected: 6

Retrocopy - Parental Gene Alignment:

Parental  EEKMFGF-HKPKMYRSIEGCCICRAKSSSSRFTDSKRYEKDFQSCFGLHETRSGDICNACVLL-VKRWKK
          E.KMF.F..KPKM..SIEGC.ICRAK...SRF..SK....DFQSC.......S...CN..VLL..KR.KK
Retrocopy E*KMFSF<YKPKMC*SIEGCSICRAKFCTSRFSYSK**K-DFQSCSLQLLKTSENFCNS*VLL<MKR*KK

Parental  LPAGSKKNWNHVVDARAG--PSLKTTLKPKKVKTL-SGNRIKSNQISKLQKEFKRHNSDAHSTTSSASPA
          LPAG.K...........G..PSL....KPKKV.....G.R..SN.I..LQ.EF..H..DAH.TTS..S..
Retrocopy LPAG*KNKTTGIML*AQGQDPSLNSVSKPKKV*SI<LGKR*RSN*IG*LQEEFQLHGPDAHHTTSITSST

Parental  QSPCYSN-QSDDGSDTEMASGSNRTPVFS-FLDLTYWKRQKICCGIIYKGRFGEVLIDTHLF-KPCCSNK
          QSPC....QSDD..DTEMASGSN.TP.FS.F......K......G..YK..FGEVL.DTHLF.KPC.S.K
Retrocopy QSPCAFT<QSDDDFDTEMASGSNNTPTFS<FVISRVGKSRNYVVGFNYKRHFGEVL-DTHLFNKPCFSSK

Parental  KAAAEKPEEQGP-EPLPISTQE
          KA..EKPE..G..EPLPIS..E
Retrocopy KAVSEKPEKCGT>EPLPISIEE

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012049_cerebellum	0.08 RPM	1.97 RPM
SRP017611_brain	0.08 RPM	0.48 RPM
SRP017611_kidney	0.00 RPM	4.62 RPM
SRP017611_liver	0.07 RPM	0.73 RPM

ChIP-Seq

Canis familiaris was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_cfam_1120 retrocopy.

TSS

Canis familiaris was not studied using FANTOM5 data.

Lab

retro_cfam_1120 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_cfam_1120 has 1 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Equus caballus	retro_ecab_385

---

Parental genes homology:

Parental genes homology involve 24 parental genes, and 70 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000001161	1 retrocopy	retro_btau_1020,
Canis familiaris	ENSCAFG00000010685	4 retrocopies	retro_cfam_1120 , retro_cfam_1552, retro_cfam_1692, retro_cfam_1837,
Choloepus hoffmanni	ENSCHOG00000007193	17 retrocopies	retro_chof_109, retro_chof_1108, retro_chof_1136, retro_chof_1255, retro_chof_1614, retro_chof_1689, retro_chof_1770, retro_chof_1971, retro_chof_2126, retro_chof_2493, retro_chof_2576, retro_chof_2587, retro_chof_453, retro_chof_511, retro_chof_856, retro_chof_979, retro_chof_998,
Callithrix jacchus	ENSCJAG00000001215	3 retrocopies	retro_cjac_1072, retro_cjac_3154, retro_cjac_3289,
Dasypus novemcinctus	ENSDNOG00000016873	2 retrocopies	retro_dnov_1674, retro_dnov_2668,
Equus caballus	ENSECAG00000009655	2 retrocopies	retro_ecab_287, retro_ecab_385,
Erinaceus europaeus	ENSEEUG00000012617	2 retrocopies	retro_eeur_350, retro_eeur_372,
Echinops telfairi	ENSETEG00000002888	2 retrocopies	retro_etel_1087, retro_etel_427,
Felis catus	ENSFCAG00000008955	4 retrocopies	retro_fcat_1311, retro_fcat_1339, retro_fcat_58, retro_fcat_965,
Homo sapiens	ENSG00000139146	3 retrocopies	retro_hsap_1928, retro_hsap_1945, retro_hsap_4029,
Gorilla gorilla	ENSGGOG00000015484	3 retrocopies	retro_ggor_1405, retro_ggor_1415, retro_ggor_2704,
Microcebus murinus	ENSMICG00000003460	2 retrocopies	retro_mmur_1176, retro_mmur_409,
Myotis lucifugus	ENSMLUG00000009827	3 retrocopies	retro_mluc_1663, retro_mluc_1672, retro_mluc_765,
Macaca mulatta	ENSMMUG00000009155	2 retrocopies	retro_mmul_1074, retro_mmul_2324,
Mus musculus	ENSMUSG00000039985	4 retrocopies	retro_mmus_1234, retro_mmus_1471, retro_mmus_1557, retro_mmus_3331,
Nomascus leucogenys	ENSNLEG00000016487	1 retrocopy	retro_nleu_1213,
Oryctolagus cuniculus	ENSOCUG00000008618	1 retrocopy	retro_ocun_1176,
Otolemur garnettii	ENSOGAG00000008927	4 retrocopies	retro_ogar_1253, retro_ogar_1762, retro_ogar_2117, retro_ogar_251,
Pongo abelii	ENSPPYG00000004396	2 retrocopies	retro_pabe_1592, retro_pabe_3309,
Rattus norvegicus	ENSRNOG00000046048	1 retrocopy	retro_rnor_2858,
Rattus norvegicus	ENSRNOG00000049943	2 retrocopies	retro_rnor_1121, retro_rnor_2917,
Sarcophilus harrisii	ENSSHAG00000006261	1 retrocopy	retro_shar_670,