RetrogeneDB ID: retro_lcha_33

Retrocopy location

• Organism: Coelacanth (Latimeria chalumnae)
• Coordinates: JH126669.1:387354..387939(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: MAD1L1
• Ensembl ID: ENSLACG00000017346
• Aliases: None
• Description: None

Sequence

>retro_lcha_33
TGGTTTAATAAGGGGTTGTATAGGATTGGGGACTTTTTTCGAGGTACCACTTTGAAATCTTTTAATGATCTCCAGAGGGA
ATTTTCTCTGGATTCTAAGGATATATGGCGCTTTTTCCAGTTAAGGCACTGTATTGTGCAGCTTTATAGTGATTGGGGGA
AGGCTCCCTTACCAGGGGAGTTTCTGCCGTTTTATGAATCGGCAAGGTGCTTGCTGCATACTGCGGCGCTTATCTACTCC
CATCTTATTTCCATTCCACCAGCAATTATGATAGGTCCGCTAGAGTATTGGGTGAGGGAGTTGGAGCTAGAACTGGAGGG
AGGAGGAAGAGAGAGCCTCATGAGAAACATGTATCTCCCCTTGAGAGAAGCCAGGCTAAAACTACAGCAATTTAAGATCA
TCAATAGGCTTTATTGGACACCGTCACGTCTTGGGGTGGCTGGGCTATGAGAAGGGAGAGGCTGCTGGTTCTGTTTAGCG
CAGGTGGGAGATCTCCATCATATGCTGTGGGAATGTGGAAACCTAACGCAATTTTGGGATGAGGTGCTGCAGTTTTGCGG
TAAGGTGCTGGACAGGTTTATTCCT

Alignment

ORF - retro_lcha_33 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 73.33 %
• Parental protein coverage: 87.84 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 0

Retrocopy - Parental Gene Alignment:

Parental  WCAESLSLLSVKYNCTTLKPFSDLHRQFCLDSKDIWRFFQLRHCIVQLYRDQGKVPLLGELLPFYELTRC
          W....L.........TTLK.F.DL.R.F.LDSKDIWRFFQLRHCIVQLY.D.GK.PL.GE.LPFYE..RC
Retrocopy WFNKGLYRIGDFFRGTTLKSFNDLQREFSLDSKDIWRFFQLRHCIVQLYSDWGKAPLPGEFLPFYESARC

Parental  LPHTAALIYSHLISIPPASMIGPLEYWVRELELELEEGERESLMRNMYLPLREARLKLQHFKIVNRLYWT
          L.HTAALIYSHLISIPPA.MIGPLEYWVRELELELE.G.RESLMRNMYLPLREARLKLQ.FKI.NRLYWT
Retrocopy LLHTAALIYSHLISIPPAIMIGPLEYWVRELELELEGGGRESLMRNMYLPLREARLKLQQFKIINRLYWT

Parental  PSHLGVAGLQEGRGCWFCSAQVGDLQHMLWECENLTRFWDEVLQFCGKVLDRPIP
          PS.LGVAGL.EGRGCWFC.AQVGDL.HMLWEC.NL...............DR.IP
Retrocopy PSRLGVAGL*EGRGCWFCLAQVGDLHHMLWECGNLXXXXXXXXXXXXXXXDRFIP

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
DRP000627_gill	0.00 RPM	1.12 RPM
DRP000627_kidney	0.00 RPM	0.58 RPM
DRP000627_pectoral_fin	0.00 RPM	3.42 RPM
DRP000627_pelvic_fin	0.00 RPM	3.94 RPM
DRP000627_pharynx	0.00 RPM	0.51 RPM
DRP000627_tail_muscle	0.00 RPM	0.43 RPM

ChIP-Seq

Latimeria chalumnae was not studied using ChIP-Seq data.

EST

Latimeria chalumnae was not studied using EST data.

TSS

Latimeria chalumnae was not studied using FANTOM5 data.

Lab

retro_lcha_33 was not experimentally validated.

Homology

Retrocopy orthology:

Latimeria chalumnae does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 2 parental genes, and 18 retrocopies.

Species	Parental gene accession	Retrocopies number
Equus caballus	ENSECAG00000014242	1 retrocopy	retro_ecab_724,
Latimeria chalumnae	ENSLACG00000017346	17 retrocopies	retro_lcha_102, retro_lcha_106, retro_lcha_109, retro_lcha_113, retro_lcha_114, retro_lcha_115, retro_lcha_33 , retro_lcha_36, retro_lcha_41, retro_lcha_44, retro_lcha_60, retro_lcha_66, retro_lcha_70, retro_lcha_79, retro_lcha_80, retro_lcha_93, retro_lcha_99,