sp|Q9LVR5|MCU3_ARATH ----> Calcium uniporter protein 3, mitochondrial

Intron 1 GTATGGTTTC ... ATGGGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.43	14.3	21	7
A2	54.55	5	6	6
A3	58.31	4.7	8	5
A4	52.94	4	3	6
A5	63.64	2	3	4
A6	30.25	17	10	4
A7	42.34	31	27	18
A8	54.76	19	25	21
A9	34.19	27	16	12
A10	51.16	22	17	29

>TRINITY_DN48846_c1_g1_i8 from sample A7 / Example spliced isoform

AGCGGGATATATGTTTCTACCTGACAGAAGTGACTGATGAAGAAGAGGACATTCAATTCGTCCTTGTCACTATTCCAGACTTCAAATGCAAGAAACGAGCAATAAACGGATAGCGGATTACATTTTTTTTCTTGCCGCAATTTTTTTTTCTTGATTCCTTGAAGTGTAAAGAAGACTCATGCCAACTGTAGTGTAAAGAA
GACTCATGCCTGGAGGAGATCACTCATGCCAACGACGAGGAGATCATAACCGTGTCCACTAACGAACAGGCGATCAAAGAAAGGAAAGAAATCAGTTTTTGCAGGAAATGCTTCATTTCTTTTCTCAATTCTGTTTGGGGAATGGCGGGATTAATGTCGAACCATTGTCTAGTAGAGGGATTTCGGGCATGCTGTTCAGT
TTCCCAAATGGTCTCTAGAGTTTTTCTATTATAGAGAAGCATGCTGGGTAGGATCTTGCTCTACTGGTAATCGGACTGGATCATAAGCAAATTCTCTCCCAATTAACATTTCCATTTCTTCTCTTTCTTCGAGAGCCACTGCAGGATTTGTATTTGTAGCCATAACTCTCCCAATTCAATTAAGGTGCCAGTAAAATTGT
CTATAAAAAGTTTAAAGTTATTCATACGTCATTTAAAAAAAAATTATCTACTCGTGAATCTAAAAAAAATATTTATTAAAAGATATTATATATTATCTACTTTCGTGAATCTAAAATATTTATTAAAAATTATCTACTCGTGAATCTAAAATATTTATTATTATCTTTTAAGAGGAAAATTACATTTTATATATTATATA
TTATATTATTATCTTCACTTCAATATCCAATCCATCTCGTGCGACTCTGTAGTGAACACGGTTGTTTCAGAATTCGGTTTAACCTCAAATCCTAAGTACCGAATGCTATCAATATCTGTTCCACTGGTATTTAACATCTCCGACTCCCTGTACGAACTCAATATCTGTTATGTTATATTATTTGTTTTGTTTTGTTTCAC
AATTTTTTAGACTACAATTTTTTACATAACAATCCATCGCCAAATTTGTTATAAAATCACGACCAAATCGGCGCCATCGACTACTTTCGAATGTAGTTATCAAATACAATCAAA

>TRINITY_DN48846_c1_g1_i6 from sample A7 / Example unspliced isoform:

AGCGGGATATATGTTTCTACCTGACAGAAGTGACTGATGAAGAAGAGGACATTCAATTCGTCCTTGTCACTATTCCAGACTTCAAATGCAAGAAACGAGCAATAAACGGATAGCGGATTACATTTTTTTTCTTGCCGCAATTTTTTTTTCTTGATTCCTTGAAGgtatggtttcttgccgcaattttttttcttgattcc
ttagatctctgaacaattgaaggtatggtttctggcctatcctgttaatgaaagaacggaaagaaagcagtatatcaatatagacttgataattataaatgaacagtaaaagacataaatcgaacgagttattggttcaggattattaatttgttctgcaatgggtgtagTGTAAAGAAGACTCATGCCAACTGTAGTGT
AAAGAAGACTCATGCCAACTGGAGGAGATCACTCATGCCAACGACGAGGAGATCATAACCGTGTCCACTAACGAACAGGCGATCAAAGAAAGGAAAGAAATCAGTTTTTGCAGGAAATGCTTCATTTCTTTTCTCAATTCTGTTTGGGGAATGGCGGGATTAATGTCGAACCATTGTCTAGTAGAGGGATTTCGGGCATG
CTGTTCAGTTTCCCAAATGGTCTCTAGAGTTTTTCTATTATAGAGAAGCATGCTGGGTAGGATCTTGCTCTACTGGTAATCGGACTGGATCATAAGCAAATTCTCTCCCAATTAACATTTCCATTTCTTCTCTTTCTTCGAGAGCCACTGCAGGATTTGTATTTGTAGCCATAACTCTCCCAATTCAATTAAGGTGCCAG
TAAAATTGTCTATAAAAAGTTTAAAGTTATTCATACGTCATTTAAAAAAAAATTATCTACTCGTGAATCTAAAAAAAATATTTATTAAAAGATATTATATATTATCTACTTTCGTGAATCTAAAATATTTATTAAAAATTATCTACTCGTGAATCTAAAATATTTATTATTATCTTTTAAGAGGAAAATTACATTTTATA
TATTATATATTATATTATTATCTTCACTTCAATATCCAATCCATCTCGTGCGACTCTGTAGTGAACACGGTTGTTTCAGAATTCGGTTTAACCTCAAATCCTAAGTACCGAATGCTATCAATATCTGTTCCACTGGTATTTAACATCTCCGACTCCCTGTACGAACTCAATATCTGTTATGTTATATTATTTGTTTTGTT
TTGTTTCACAATTTTTTAGACTACAATTTTTTACATAACAATCCATCGCCAAATTTGTTATAAAATCACGACCAAATCGGCGCCATCGACTACTTTCGAATGTAGTTATCAAATACAATCAAA

Intron 2 GTATGGTTTC ... TAATGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.82	7	11.3	1
A2	52.33	4	7.3	2
A3	100	0	4.7	3
A4	77.78	1	2.3	5
A5	35.24	2	2.3	0
A6	14.25	20	1.7	5
A7	15.7	34	5.7	7
A8	35.67	23	17.7	8
A9	14.57	55.7	8	11
A10	24.04	20.3	9	4

>TRINITY_DN48846_c1_g1_i6 from sample A7 / Example spliced isoform

AGCGGGATATATGTTTCTACCTGACAGAAGTGACTGATGAAGAAGAGGACATTCAATTCGTCCTTGTCACTATTCCAGACTTCAAATGCAAGAAACGAGCAATAAACGGATAGCGGATTACATTTTTTTTCTTGCCGCAATTTTTTTTTCTTGATTCCTTGAAGGTATGGTTTCTTGCCGCAATTTTTTTTCTTGATTCC
TTAGATCTCTGAACAATTGAAGGTATGGTTTCTGGCCTATCCTGTTAATGAAAGAACGGAAAGAAAGCAGTATATCAATATAGACTTGATAATTATAAATGAACAGTAAAAGACATAAATCGAACGAGTTATTGGTTCAGGATTATTAATTTGTTCTGCAATGGGTGTAGTGTAAAGAAGACTCATGCCAACTGTAGTGT
AAAGAAGACTCATGCCAACTGGAGGAGATCACTCATGCCAACGACGAGGAGATCATAACCGTGTCCACTAACGAACAGGCGATCAAAGAAAGGAAAGAAATCAGTTTTTGCAGGAAATGCTTCATTTCTTTTCTCAATTCTGTTTGGGGAATGGCGGGATTAATGTCGAACCATTGTCTAGTAGAGGGATTTCGGGCATG
CTGTTCAGTTTCCCAAATGGTCTCTAGAGTTTTTCTATTATAGAGAAGCATGCTGGGTAGGATCTTGCTCTACTGGTAATCGGACTGGATCATAAGCAAATTCTCTCCCAATTAACATTTCCATTTCTTCTCTTTCTTCGAGAGCCACTGCAGGATTTGTATTTGTAGCCATAACTCTCCCAATTCAATTAAGGTGCCAG
TAAAATTGTCTATAAAAAGTTTAAAGTTATTCATACGTCATTTAAAAAAAAATTATCTACTCGTGAATCTAAAAAAAATATTTATTAAAAGATATTATATATTATCTACTTTCGTGAATCTAAAATATTTATTAAAAATTATCTACTCGTGAATCTAAAATATTTATTATTATCTTTTAAGAGGAAAATTACATTTTATA
TATTATATATTATATTATTATCTTCACTTCAATATCCAATCCATCTCGTGCGACTCTGTAGTGAACACGGTTGTTTCAGAATTCGGTTTAACCTCAAATCCTAAGTACCGAATGCTATCAATATCTGTTCCACTGGTATTTAACATCTCCGACTCCCTGTACGAACTCAATATCTGTTATGTTATATTATTTGTTTTGTT
TTGTTTCACAATTTTTTAGACTACAATTTTTTACATAACAATCCATCGCCAAATTTGTTATAAAATCACGACCAAATCGGCGCCATCGACTACTTTCGAATGTAGTTATCAAATACAATCAAA

>TRINITY_DN48846_c1_g1_i4 from sample A7 / Example unspliced isoform:

AGCGGGATATATGTTTCTACCTGACAGAAGTGACTGATGAAGAAGAGGACATTCAATTCGTCCTTGTCACTATTCCAGACTTCAAATGCAAGAAACGAGCAATAAACGGATAGCGGATTACATTTTTTTTCTTGCCGCAATTTTTTTTTCTTGATTCCTTGAAGTGTAAAGAAGACTCATGCCAACTGTAGTGTAAAGAA
GACTCATGCCAACTGGAGGAGATCACTCATGCCAACGACGAGGAGATCATAACCGTGTCCACTAACGAACAGGCGATCAAAGAAAGGAAAGAAATCAGgtatggtttcgtgatctgacttctttgtgtcgatagttaggaatgaatgcatttctttgtgtccatttcgaacgtcaatttcatgaaaaatggtagagaatg
gtcgcaaaaccagggattggatttttacatagccttcactattattataaacaaagagtagtaatcctaaaatcacaccccgtcctggccaggaattttcttcatagttctcaagaaatcccttggaattttctccacgtgctaatatgacgtcaattgccctaactatttttttacaagcatcaagagctgcttctgtg
caatccttttcttgtagggtttcctcaataactttccttaaatatgtgaagacttgtaaagatatgaataattttatgttctgagaatgaaactcacaaaattagttttcttcaatatttttgcccattgattctctgttattgtacttccttcaacatatttatgcaaattcattaaatgttgttccatctctccctgc
tccacaatttttgaatactcggtaatgtacagTTTTTGCAGGAAATGCTTCATTTCTTTTCTCAATTCTGTTTGGGGAATGGCGGGATTAATGTCGAACCATTGTCTAGTAGAGGGATTTCGGGCATGCTGTTCAGTTTCCCAAATGGTCTCTAGAGTTTTTCTATTATAGAGAAGCATGCTGGGTAGGATCTTGCTCTA
CTGGTAATCGGACTGGATCATAAGCAAATTCTCTCCCAATTAACATTTCCATTTCTTCTCTTTCTTCGAGAGCCACTGCAGGATTTGTATTTGTAGCCATAACTCTCCCAATTCAATTAAGGTGCCAGTAAAATTGTCTATAAAAAGTTTAAAGTTATTCATACGTCATTTAAAAAAAAATTATCTACTCGTGAATCTAA
AAAAAATATTTATTAAAAGATATTATATATTATCTACTTTCGTGAATCTAAAATATTTATTAAAAATTATCTACTCGTGAATCTAAAATATTTATTATTATCTTTTAAGAGGAAAATTACATTTTATATATTATATATTATATTATTATCTTCACTTCAATATCCAATCCATCTCGTGCGACTCTGTAGTGAACACGGTT
GATGGTTTCATGATCTCTGACTTTTTCAGAATTCGGTTTAACCTCAAA