sp|P24465|C71A1_PERAE ----> Cytochrome P450 71A1

Intron 1 GTAAAATTTG ... ACTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	-1	0	0	0
A4	-1	0	0	0
A5	50	1	1	0
A6	46.88	17	15	0
A7	43.33	17	13	5
A8	33.33	18	9	2
A9	45.45	48	40	11
A10	32.69	35	17	10

>TRINITY_DN46285_c2_g1_i6 from sample A7 / Example spliced isoform

CTGGAGATGATCTGAATTCCAAGTTCCATGCTCATGAATTCCAAATATTTGAGGATTACTCTCCCTGATCTCTTGATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATTTGGG
CTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTCAGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGATCGA
TGATGGTTAACCTCATATTTGATATCATTTTACGACTAATCCTACCTGGTTCTTTGCGATTTTATTTCTTGATGCTGTGGTGGTACTGGTAGGCATCGCCTCATTTAATCTGTGGGAGATGCGTTTTTATTGAGGAAAATAGGTTGTCATATGATCGAGCTATTTTATTTTGAATGATTCATGGTAAATACATTGAAATT
ATTTTGTTTTGATTTACAATTGCACAGCAATGAAGCAATGAAGAAGACAAATTGGCACTCAATTCTATAGAAAGCGTATAGCACATTTGATCTCTATGTCGTCCTGGGCCTTGGAATTGTGGGTCTTCTCTGATCCGGATTTTATGAGCAGAACTAAATGAATACAGATTATTATCAGATGCTTAACACAAAATAATAGA
GATATAGTAAAATTGGTTTTTTATTCAATAAGGAAATAAGCTAAAACCGGAAAACAAGCTTGTTGGAATGGTGGCGGCTTATTTTTGAGAAGAGATGTGACAGTTGTGCTCATTTCCC

>TRINITY_DN46285_c2_g1_i17 from sample A7 / Example unspliced isoform:

CTGGAGATGATCTGAATTCCAAGTTCCATGCTCATGAATTCCAAATATTTGAGGATTACTCTCCCTGATCTCTTGATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATTTGGG
CTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTCAGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGATCGA
TGATGGTTAACCTCATATTTGATATCATTTTACGACTAATCCTACCTGgtaaaatttgcaccgaatgcttatgcgtttatcgaatttgaatcaatagattcagtctcaaagtttattaaggttttctttaactatcttgcatttttagggcttaatttaggatcggtttaaaaaaacaactaaactttttcagGTTCTTT
GCGATTTTATTTCTTGATGCTGTGGTGGTACTGGTAGGCATCGCCTCATTTAATCTGTGGGAGATGCGTTTTTATTGAGGAAAATAGGTTGTCATATGATCGAGCTATTTTATTTTGAATGATTCATGGTAAATACATTGAAATTATTTTGTTTTGATTTACAATTGCACAGCAATGAAGCAATGAAGAAGACAAATTGG
CACTCAATTCTATAGAAAGCGTATAGCACATTTGATCTCTATGTCGTCCTGGGCCTTGGAATTGTGGGTCTTCTCTGATCCGGATTTTATGAGCAGAACTAAATGAATACAGATTATTATCAGATGCTTAACACAAAATAATAGAGATATAGTAAAATTGGTTTTTTATTCAATAAGGAAATAAGCTAAAACCGGAAAAC
AAGCTTGTTGGAATGGTGGCGGCTTATTTTTGAGAAGAGATGTGACAGTTGTGCTCATTTCCC

Intron 2 GTTCTTTGCG ... AATTGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	-1	0	0	0
A4	-1	0	0	0
A5	33.33	1	1	0
A6	54.17	11	17	9
A7	58.49	11	17	14
A8	63.33	11	18	20
A9	53.66	38	48	40
A10	63.89	13	35	11

>TRINITY_DN44408_c0_g1_i3 from sample A8 / Example spliced isoform

CAAATATTTGAGGATTACTCTCCTTGTTCTCTCTATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATTTGGGCTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTC
AGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGATCGATGATGGTTAACCTCATATTTGATATCATTTTACGACTAATC
CTACCTGCAATGAAGCAATGAAGAAGACAAATTGGCACTCAATTCTATAGAAAGCGTATAGCACATTTGATCTCTATGTCGTCCTGGGCCTTGGAATTGTGGGTCTTCTCTGATCCGGATTTTATGAGCAGAACTAAATGAATACAGATTATTATCAGATGCTTAACACAAAATAATAGAGATATAGTAAAATTGGTTTT
TTATTCAATAAGGAAATAAGCTAAAACCGGAAAACAAGCTTGTTGGAATCGT

>TRINITY_DN44408_c0_g1_i18 from sample A8 / Example unspliced isoform:

CAAATATTTGAGGATTACTCTCCTTGTTCTCTCTATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATTTGGGCTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTC
AGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGATCGATGATGGTTAACCTCATATTTGATATCATTTTACGACTAATC
CTACCTGgttctttgcgattttatttcttgatgctgtggtggtactggtaggcatcgcctcatttaatctgtgggagatgcgtttttattgaggaaaataggttgtcatatgatcgagctattttattttgaatgattcatggtaaatacattgaaattattttgttttgatttacaattgcacagCAATGAAGCAATGA
AGAAGACAAATTGGCACTCAATTCTATAGAAAGCGTATAGCACATTTGATCTCTATGTCGTCCTGGGCCTTGGAATTGTGGGTCTTCTCTGATCCGGATTTTATGAGCAGAACTAAATGAATACAGATTATTATCAGATGCTTAACACAAAATAATAGAGATATAGTAAAATTGGTTTTTTATTCAATAAGGAAATAAGC
TAAAACCGGAAAACAAGCTTGTTGGAATCGT

Intron 3 GTCTTATCCC ... GTCTTTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	-1	0	0	0
A4	-1	0	0	0
A5	33.33	1	1	0
A6	30.61	17	12	3
A7	26.32	21	12	3
A8	34.62	17	9	9
A9	36	40	31	14
A10	27.66	17	13	0

>TRINITY_DN44408_c0_g1_i19 from sample A8 / Example spliced isoform

CAAATATTTGAGGATTACTCTCCTTGTTCTCTCTATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATTTGGGCTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTC
AGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGATCGATGATGGTTAACCTCATATTTGATATCATTTTACGACTAATC
CTACCTGAAAATTTACTTTTTCAGAAAGCCGTGGATTGCCCATAGAGAGAATCTTCCTGTCAGTTTCAAGAGCGAGAGAAGAATTGGAGGAAACGACTTGCCGTCTTCTGGAAGAGAGAGTAAGCATCATGATTAAAGAAGAAATCACAAGGAAGTTGCGATGGCTGCTGTGCGAAACTCAAGCAACAATTTGTTAATTT
GTTATGTTGTTGCAAATGTTGCAGATAAGTTTAGAGACTGTGGTTGTACAGCACAACTTGTAGTTTACCAAAGTTGGTTCTGATAGACTAGATTTTGGGTTCCACTATCGCTTTTGTATTAGAACTGTGAACAATCTGTTAGGAATTTGAGGCTAAGATTTAAG

>TRINITY_DN44408_c0_g1_i7 from sample A8 / Example unspliced isoform:

CAAATATTTGAGGATTACTCTCCTTGTTCTCTCTATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATTTGGGCTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTC
AGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGATCGATGATGGTTAACCTCATATTTGATATCATTTTACGACTAATC
CTACCTGgtcttatcccactatgtttattcaaaaataaattgttcttccacctgagaggttattgtctttttagAAAATTTACTTTTTCAGAAAGCCGTGGATTGCCCATAGAGAGAATCTTCCTGTCAGTTTCAAGAGCGAGAGAAGAATTGGAGGAAACGACTTGCCGTCTTCTGGAAGAGAGAGTAAGCATCATGAT
TAAAGAAGAAATCACAAGGAAGTTGCGATGGCTGCTGTGCGAAACTCAAGCAACAATTTGTTAATTTGTTATGTTGTTGCAAATGTTGCAGATAAGTTTAGAGACTGTGGTTGTACAGCACAACTTGTAGTTTACCAAAGTTGGTTCTGATAGACTAGATTTTGGGTTCCACTATCGCTTTTGTATTAGAACTGTGAACA
ATCTGTTAGGAATTTGAGGCTAAGATTTAAG

Intron 4 GTTCTTTGCG ... ACTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	1
A3	-1	0	0	0
A4	-1	0	0	0
A5	50	1	1	0
A6	53.13	15	17	3
A7	45.95	20	17	7
A8	48.65	19	18	10
A9	53.93	41	48	20
A10	67.31	17	35	2

>TRINITY_DN43200_c0_g6_i1 from sample A9 / Example spliced isoform

CGGGCTGGAGATGATCTGAATTCCAAGTTCCATGCTCATGAATTCCAAATATTTGAGGATTACTCTCCTTGTTCTCTCTATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATT
TGGGCTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTCAGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGA
TCGATGATGGTTAACCTCATATTTGATATCATTTTACGACTAATCCTACCTGAAAGCCGTGGATTGCCCATAGAGAGAATCTTCCTGTCAGTTTCAAGAGCGAGAGAAGAATTGGAGGAAACGACTTGCCGTCTTCTGGAAGAGAGAGTAAGCATCATGATTAAAGAAGAAATCACAAGGAAGTTGCGATGGCTGCTGTG
CGAAACTCAAGCAACAATTTGTTAATTTGTTATGTTGTTGCAAATGTTGCAGATAAGTTTAGAGACTGTGGTTGTACAGCACAACTTGTAGTTTACCAAAGTTGGTTCTGATAGACTAGATTTTGGGTTCCACTATCGCTTTTGTATTAGAACTGTGAACAATCTGTTAGGAATTTGAGGCTAAGATTTAAG

>TRINITY_DN43200_c0_g6_i3 from sample A9 / Example unspliced isoform:

CGGGCTGGAGATGATCTGAATTCCAAGTTCCATGCTCATGAATTCCAAATATTTGAGGATTACTCTCCTTGTTCTCTCTATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATT
TGGGCTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTCAGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGA
TCGATGATGGTTAACCTCATATTTGATATCATTTTACGACTAATCCTACCTGgttctttgcgattttatttcttgatgctgtggtggtactggtaggcatcgcctcatttaatctgtgggagatgcgtttttattgaggaaaataggttgtcatatgatcgagctattttattttgaatgattcatggtaaatacattga
aattattttgttttgatttacaattgcacagcaatgaagcaatgaagaagacaaattggcactcaattctatagaaagcgtatagcacatttgatctctatgtcgtcctgggccttggaattgtgggtcttctctgatccggattttatgagcagaactaaatgaatacagattattatcagatgcttaacacaaaataa
tagagatatagtaaaattggttttttattcaataaggaaataagctaaaaccggaaaacaagcttgttggaatggtggcggcttatttttgagaagagatgtgacagttgtgctcatttcccaagaagatggttacaatgaggctctcaaggccttttacaataaacatgaatgttaaaaaccctagacatccaaaaacc
taaccctaggagatgaagcgccaaaagtcgtttggttaagttattacatcaacttccccgggtcgtaacttttgcatacgatctccgtttgggtgctcgatatcccattggaaatgtttatccaagatttacaactttcgactcaagccaaatgcatgccaaaggaaaccatttggccccccaaatcgccaaaaaaagtc
gtttaggcctcaaaatggccccggaagtacccaaaaaactactaaaaatagtacaataccaaaattgcgagaaaactgttttgagccaattggaaaggtgagtgtccaccgatagatcagtctcctccatatgtaatgtgttgtctatgacatttgattgtgttaaaatattgtttgataataaaattgtgttcggtatt
ccccttagggtttgtagggttggtataatgagcattcatatcatttaaatatgccttttcttttcgattagttatagtttttattttgtatgggatatttgtcgttgtagagtgctaacacatagtctagcatcttcgtcatcccatgagcatggcagccatggatatttgaagctgttacacttattattttttgaatg
gaaagactgttgttgtaaatatgatcttgagtagcaaggatatctatgcagatattgtgataaatcatattttccattaaacactatcaaactataattgattaaatgttttttatgtgtatccatccaatggttttttgtgtagccatcagattgtttaaatgtttaatgattttaatgtgtagacatccttatcccca
attgtgttttgggacaatcggaggttgagaagtcaatggttgtctttcttagcttttgcaaaattgcgcaggtcttatcccactatgtttattcaaaaataaattgttcttccacctgagaggttattgtctttttagaaaatttactttttcagAAAGCCGTGGATTGCCCATAGAGAGAATCTTCCTGTCAGTTTCAA
GAGCGAGAGAAGAATTGGAGGAAACGACTTGCCGTCTTCTGGAAGAGAGAGTAAGCATCATGATTAAAGAAGAAATCACAAGGAAGTTGCGATGGCTGCTGTGCGAAACTCAAGCAACAATTTGTTAATTTGTTATGTTGTTGCAAATGTTGCAGATAAGTTTAGAGACTGTGGTTGTACAGCACAACTTGTAGTTTACC
AAAGTTGGTTCTGATAGACTAGATTTTGGGTTCCACTATCGCTTTTGTATTAGAACTGTGAACAATCTGTTAGGAATTTGAGGCTAAGATTTAAG

Intron 5 GTAAAATTTG ... ACTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	100	0	0	1
A3	-1	0	0	0
A4	-1	0	0	0
A5	33.33	1	1	0
A6	37.5	15	15	3
A7	33.33	20	13	7
A8	33.33	19	9	10
A9	42.25	41	40	20
A10	35.85	17	17	2

>TRINITY_DN43200_c0_g6_i1 from sample A9 / Example spliced isoform

CGGGCTGGAGATGATCTGAATTCCAAGTTCCATGCTCATGAATTCCAAATATTTGAGGATTACTCTCCTTGTTCTCTCTATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATT
TGGGCTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTCAGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGA
TCGATGATGGTTAACCTCATATTTGATATCATTTTACGACTAATCCTACCTGAAAGCCGTGGATTGCCCATAGAGAGAATCTTCCTGTCAGTTTCAAGAGCGAGAGAAGAATTGGAGGAAACGACTTGCCGTCTTCTGGAAGAGAGAGTAAGCATCATGATTAAAGAAGAAATCACAAGGAAGTTGCGATGGCTGCTGTG
CGAAACTCAAGCAACAATTTGTTAATTTGTTATGTTGTTGCAAATGTTGCAGATAAGTTTAGAGACTGTGGTTGTACAGCACAACTTGTAGTTTACCAAAGTTGGTTCTGATAGACTAGATTTTGGGTTCCACTATCGCTTTTGTATTAGAACTGTGAACAATCTGTTAGGAATTTGAGGCTAAGATTTAAG

>TRINITY_DN43200_c0_g6_i8 from sample A9 / Example unspliced isoform:

CGGGCTGGAGATGATCTGAATTCCAAGTTCCATGCTCATGAATTCCAAATATTTGAGGATTACTCTCCTTGTTCTCTCTATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATT
TGGGCTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTCAGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGA
TCGATGATGGTTAACCTCATATTTGATATCATTTTACGACTAATCCTACCTGgtaaaatttgcaccgaatgcttatgcgtttatcgaatttgaatcaatagattcagtctcaaagtttattaaggttttctttaactatcttgcatttttagggcttaatttaggatcggtttaaaaaaacaactaaactttttcaggtt
ctttgcgattttatttcttgatgctgtggtggtactggtaggcatcgcctcatttaatctgtgggagatgcgtttttattgaggaaaataggttgtcatatgatcgagctattttattttgaatgattcatggtaaatacattgaaattattttgttttgatttacaattgcacagcaatgaagcaatgaagaagacaaa
ttggcactcaattctatagaaagcgtatagcacatttgatctctatgtcgtcctgggccttggaattgtgggtcttctctgatccggattttatgagcagaactaaatgaatacagattattatcagatgcttaacacaaaataatagagatatagtaaaattggttttttattcaataaggaaataagctaaaaccgga
aaacaagcttgttggaatggtggcggcttatttttgagaagagatgtgacagttgtgctcatttcccaagaagatggttacaatgaggctctcaaggccttttacaataaacatgaatgttaaaaaccctagacatccaaaaacctaaccctaggagatgaagcgccaaaagtcgtttggttaagttattacatcaactt
ccccgggtcgtaacttttgcatacgatctccgtttgggtgctcgatatcccattggaaatgtttatccaagatttacaactttcgactcaagccaaatgcatgccaaaggaaaccatttggccccccaaatcgccaaaaaaagtcgtttaggcctcaaaatggccccggaagtacccaaaaaactactaaaaatagtaca
ataccaaaattgcgagaaaactgttttgagccaattggaaaggtgagtgtccaccgatagatcagtctcctccatatgtaatgtgttgtctatgacatttgattgtgttaaaatattgtttgataataaaattgtgttcggtattccccttagggtttgtagggttggtataatgagcattcatatcatttaaatatgcc
ttttcttttcgattagttatagtttttattttgtatgggatatttgtcgttgtagagtgctaacacatagtctagcatcttcgtcatcccatgagcatggcagccatggatatttgaagctgttacacttattattttttgaatggaaagactgttgttgtaaatatgatcttgagtagcaaggatatctatgcagatat
tgtgataaatcatattttccattaaacactatcaaactataattgattaaatgttttttatgtgtatccatccaatggttttttgtgtagccatcagattgtttaaatgtttaatgattttaatgtgtagacatccttatccccaattgtgttttgggacaatcggaggttgagaagtcaatggttgtctttcttagctt
ttgcaaaattgcgcaggtcttatcccactatgtttattcaaaaataaattgttcttccacctgagaggttattgtctttttagaaaatttactttttcagAAAGCCGTGGATTGCCCATAGAGAGAATCTTCCTGTCAGTTTCAAGAGCGAGAGAAGAATTGGAGGAAACGACTTGCCGTCTTCTGGAAGAGAGAGTAAG
CATCATGATTAAAGAAGAAATCACAAGGAAGTTGCGATGGCTGCTGTGCGAAACTCAAGCAACAATTTGTTAATTTGTTATGTTGTTGCAAATGTTGCAGATAAGTTTAGAGACTGTGGTTGTACAGCACAACTTGTAGTTTACCAAAGTTGGTTCTGATAGACTAGATTTTGGGTTCCACTATCGCTTTTGTATTAGAA
CTGTGAACAATCTGTTAGGAATTTGAGGCTAAGATTTAAG

Intron 6 GTAAAATTTG ... AATTGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	-1	0	0	0
A4	-1	0	0	0
A5	33.33	1	1	0
A6	52.17	11	15	9
A7	55.1	11	13	14
A8	56.86	11	9	20
A9	51.28	38	40	40
A10	51.85	13	17	11

>TRINITY_DN44408_c0_g1_i3 from sample A8 / Example spliced isoform

CAAATATTTGAGGATTACTCTCCTTGTTCTCTCTATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATTTGGGCTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTC
AGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGATCGATGATGGTTAACCTCATATTTGATATCATTTTACGACTAATC
CTACCTGCAATGAAGCAATGAAGAAGACAAATTGGCACTCAATTCTATAGAAAGCGTATAGCACATTTGATCTCTATGTCGTCCTGGGCCTTGGAATTGTGGGTCTTCTCTGATCCGGATTTTATGAGCAGAACTAAATGAATACAGATTATTATCAGATGCTTAACACAAAATAATAGAGATATAGTAAAATTGGTTTT
TTATTCAATAAGGAAATAAGCTAAAACCGGAAAACAAGCTTGTTGGAATCGT

>TRINITY_DN44408_c0_g1_i12 from sample A8 / Example unspliced isoform:

CAAATATTTGAGGATTACTCTCCTTGTTCTCTCTATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATTTGGGCTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTC
AGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGATCGATGATGGTTAACCTCATATTTGATATCATTTTACGACTAATC
CTACCTGgtaaaatttgcaccgaatgcttatgcgtttatcgaatttgaatcaatagattcagtctcaaagtttattaaggttttctttaactatcttgcatttttagggcttaatttaggatcggtttaaaaaaacaactaaactttttcaggttctttgcgattttatttcttgatgctgtggtggtactggtaggcat
cgcctcatttaatctgtgggagatgcgtttttattgaggaaaataggttgtcatatgatcgagctattttattttgaatgattcatggtaaatacattgaaattattttgttttgatttacaattgcacagCAATGAAGCAATGAAGAAGACAAATTGGCACTCAATTCTATAGAAAGCGTATAGCACATTTGATCTCTA
TGTCGTCCTGGGCCTTGGAATTGTGGGTCTTCTCTGATCCGGATTTTATGAGCAGAACTAAATGAATACAGATTATTATCAGATGCTTAACACAAAATAATAGAGATATAGTAAAATTGGTTTTTTATTCAATAAGGAAATAAGCTAAAACCGGAAAACAAGCTTGTTGGAATCGT

Intron 7 GTCTTATCCC ... ACTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	-1	0	0	0
A4	-1	0	0	0
A5	50	1	1	0
A6	44.44	15	12	2
A7	36.83	20	11.7	2
A8	31.3	19	8.7	8
A9	41.94	41	29.7	10.3
A10	43.33	17	13	1.3

>TRINITY_DN46285_c2_g1_i4 from sample A7 / Example spliced isoform

CTGGAGATGATCTGAATTCCAAGTTCCATGCTCATGAATTCCAAATATTTGAGGATTACTCTCCCTGATCTCTTGATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATTTGGG
CTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTCAGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGATCGA
TGATGGTTAACCTCATATTTGATATCATTTTACGACTAATCCTACCTGAAAGCCGTGGATTGCCCATAGAGAGAATCTTCCTGTCAGTTTCAAGAGCGAGAGAAGAATTGGAGGAAACGACTTGCCGTCTTCTGGAAGAGAGAGTAAGCATCATGATTAAAGAAGAAATCACAAGGAAGTTGCGATGGCTGCTGTGCGAA
ACTCAAGCAACAATTTGTTAATTTGTTATGTTGTTGCAAATGTTGCAGATAAGTTTAGAGACTGTGGTTGTACAGCACAACTTGTAGTTTACCAAAGTTGGTTCTGATAGACTAGATTTTGGGTTCCACTATCGCTTTTGTATTAGAACTGTGAACAATCTGTTAGGAATTTGAGGCTAAGATTTAAGACTT

>TRINITY_DN46285_c2_g1_i5 from sample A7 / Example unspliced isoform:

CTGGAGATGATCTGAATTCCAAGTTCCATGCTCATGAATTCCAAATATTTGAGGATTACTCTCCCTGATCTCTTGATCTCTTAAATTTTTGGACTGTTGAGGATTACTCTACTGATCTGATCGTGGACTGATTCTGTATCTCTCAATCGCAAACTGAACTTTGCTTCCAGGCCACGGTCGACGGCAGCTAAGAATTTGGG
CTACGATTGTACAATGCTGGGCCTCGATCCATATGACCGTCAGTGAACATTCGCAAGCTCTGCACATCGCATCTTCTATCTCCTTCCATGGTGGAGTCGTCTCAACATATTCTCAGGGAGGAGGTGTCCAATTTGGTGCGTGGCCTGTTCCAGAGCTGTACGCAGATGGGAATCAACCTCATATTTGGACGTGAGATCGA
TGATGGTTAACCTCATATTTGATATCATTTTACGACTAATCCTACCTGgtcttatcccactatgtttattcaaaaataaattgttcttccacctgagaggttattgtctttttagaaaatttactttttcagAAAGCCGTGGATTGCCCG