sp|Q84L08|GFT1_ARATH ----> GDP-fucose transporter 1

Intron 1 GTACACATTG ... CATCTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.22	10	10	42
A2	69.7	5	3	20
A3	61.19	13	19	22
A4	67.74	10	14	28
A5	71.43	6	13	17
A6	67.82	339	629	800
A7	63.85	233	409	414
A8	65.84	275	481	579
A9	65.77	260	485	514
A10	68.03	141	276	324

>TRINITY_DN104280_c0_g1_i5 from sample A3 / Example spliced isoform

GAAAATTCCGACATATGCACAAGTGTGGACAAAATCACCGAACCTCATTGAAATAGTGGTAGGTTATCGTTTAAAGCGGGACACAATTTCCCAAACAAAGAATCGAGTCATCCTCTTATATATTCAGCCATTTTCATGCATTCGAGACAAACACAGTATGCATGACAAAGGTTTCAGCCATTCGAGACAAACGGAACAGG
ACGAATGACAATAGTTTGGGCCATTTTCATTCATTTGACACAAATATGTAGCGAAAGTACTTAAAAAAGAGGTCAAACCCAGGATTTCTGCCAGCAAAGCAGGCGGGTCAAGCTATTCACACCAGGTACTACTTGTGGTTCTCTTCGCTTTTCATGGCTGATTCATTTTTCTTCACGTTTATACATGCAATGGATCTCAA
AGAGCTTAAAATCCCCTCATTGATTGCGTGAAGTCTACGTTCCTAACGAAGCAGATTTGAAGTAAAAGCATCGAAAAACCCTCGGTAGAAGGTTTCCAGAGCTCGCATTGGATACCCACATCCAGATCTAGGAAAAAATGGCGGGTAGATCAGAATCTTCGAAGCAATACTATGCAACGGCCACCGTCATTGTAGGCTAT
GCTCTGTGCTCTAGTTTGCTTGCAGTTATAAACAAATATGCAATCACTAAGTTCAATTACCCAGGCTTGATGACTGCCCTACAGTACTTGACGAGCGTAATTGGTGTTTGGGTTTTTGGCAAATTGGGTTTGTTGCATCACGATCCATTTGTTTGGGAAACTGCTAAGAAATTCATGCCAGCTGCATTTGTTTTTTACTT
GGCAATATTCACTAATACAAACCTTCTCAAGCATGCCAATGTAGATACATTCATTGTGTTCAGATCTCTGACCCCACTTCTTGTAGCCATAGCTGATACTGCATTCAGGAAGCAGCCATGGCCTTCTAAGTATACTTTTATGAGCCTAGTGGTTATATTGGGGGGTGCAGTTGGGTATGTAGCAACTGATTCGGGCTTTA
CACTGACTGCATATTCATGGGCTTTTGCTTATCTGATCACCATTGTTACAGAGATGGTTTACATCAAACACATGGTTACAAATCTGGGTCTGAATACATGGGGGTTTGTGTTGTACAACAATTTGCTGTCTCTGATGCTGGCACCGGTGTTCTGGATCTTGACTGGGGAGTATGTGGAAGTGTTTTCTGCTGCAGCGGAT
GCTGGTATGAGAGGTCTGTTGATATATGATTCCATATTTGCAGTGGGCCTGTCCTGTGTTTTTGGCCTTGCCATCAGCTTTTTTGGTTTTGCAGCGAGGAAGGCGGTGACAGCAACCGCATTCACTGTGACTGGGGTGGTGAACAAGTTCCTGACTGTGGTTATCAATGTGCTGATCTGGGACAAGCATGCCACTCCTGT
TGGTCTTATCTTCCTCCTTATGACTATTGTGGGTGGGGTGCTCTATCAGCAATCTGTGACCGTTGCTCCCGCCCCTCATATTGACAGCGGGATGAGTAAGCAGATGGAAGGTGTGAAGGATCATGAAGATGCTGACGTGAGTGATGAGGAAAAGAGTCTTATGAGAAAGTCTTCTGGAATTTAATTGGCTGTAGAGAAAC
ATGAGGCGATGAAGATTGGGCTGGTGATCCTTAGTAGGCCAAGTATTCGTTCTCTCTTAACATGATGCATAACCAATGACTGTCGTAAAGAAGACCAGGGACCTGTAGGGTGTGAAGATGCATAAGTCTTATTTTCATTGGTTGCTATTTAACTGTTTCCTGATGTTGAGCTCCCACTGAGCATCTGTTTCTTGAATTTT
AACTTGTGGATCTCTTACACATGAGAATGAGTCAAATGCAGCATGATGTTGTTTACTCACTTATATAAAGTAGCACTGTAATTTCAAATTTGTCTATTTTGTGTTGTGGAAAATAAGTAGAATGCAAACTTACGTTGCTTCTGTCTCTATGGCCACTCTCTATTGTTTCAAAGCTCTTAGAATTTAGATGTTGGATCAGA
ATGTTTCAGTTTAGTTGCCCTACACTGTTTGTAACCTAAAATTTGGGAAAAAATGCAATCATATGTTTGTTTTCAATAAATTGTGATCTTGGAGGAGGC

>TRINITY_DN104280_c0_g1_i2 from sample A3 / Example unspliced isoform:

GAAAATTCCGACATATGCACAAGTGTGGACAAAATCACCGAACCTCATTGAAATAGTGGTAGGTTATCGTTTAAAGCGGGACACAATTTCCCAAACAAAGAATCGAGTCATCCTCTTATATATTCAGCCATTTTCATGCATTCGAGACAAACACAGTATGCATGACAAAGGTTTCAGCCATTCGAGACAAACGGAACAGG
ACGAATGACAATAGTTTGGGCCATTTTCATTCATTTGACACAAATATGTAGCGAAAGTACTTAAAAAAGAGGTCAAACCCAGGATTTCTGCCAGCAAAGCAGGCGGGTCAAGCTATTCACACCAGGTACTACTTGTGGTTCTCTTCGCTTTTCATGGCTGATTCATTTTTCTTCACGTTTATACATGCAATGGATCTCAA
AGAGCTTAAAATCCCCTCATTGATTGCGTGAAGTCTACGTTCCTAACGAAGCAGATTTGAAGTAAAAGCATCGAAAAACCCTCGGTAGAAGGTTTCCAGAGCTCGCATTGGATACCCACATCCAGATCTAGGAAAAAATGGCGGGTAGATCAGAATCTTCGAAGCAATACTATGCAACGGCCACCGTCATTGTAGGCTAT
GCTCTGTGCTCTAGTTTGCTTGCAGTTATAAACAAATATGCAATCACTAAGTTCAATTACCCAGGCTTGATGACTGCCCTACAGTACTTGACGAGCGTAATTGGTGTTTGGGTTTTTGGCAAATTGGGTTTGTTGCATCACGATCCATTTGTTTGGGAAACTGCTAAGAAATTCATGCCAGCTGCATTTGTTTTTTACTT
GGCAATATTCACTAATACAAACCTTCTCAAGCATGCCAATGTAGATACATTCATTGTGTTCAGATCTCTGACCCCACTTCTTGTAGCCATAGCTGATACTGCATTCAGGAAGCAGCCATGGCCTTCTAAGTATACTTTTATGAGCCTAGTGGTTATATTGGGGGGTGCAGTTGGGTATGTAGCAACTGATTCGGGCTTTA
CACTGACTGCATATTCATGGGCTTTTGCTTATCTGATCACCATTGTTACAGAGATGGTTTACATCAAACACATGGTTACAAATCTGGGTCTGAATACATGGGGGTTTGTGTTGTACAACAATTTGCTGTCTCTGATGCTGGCACCGGTGTTCTGGATCTTGACTGGGGAGTATGTGGAAGTGTTTTCTGCTGCAGCGGAT
GCTGGTATGAGAGGTCTGTTGATATATGATTCCATATTTGCAGTGGGCCTGTCCTGTGTTTTTGGCCTTGCCATCAGCTTTTTTGGTTTTGCAGCGAGGAAGGCGGTGACAGCAACCGCATTCACTGTGACTGGGGTGGTGAACAAGTTCCTGACTGTGGTTATCAATGTGCTGATCTGGGACAAGCATGCCACTCCTGT
TGGTCTTATCTTCCTCCTTATGACTATTGTGGGTGGGGTGCTCTATCAGCAATCTGTGACCGTTGCTCCCGCCCCTCATATTGACAGCGGGATGAGTAAGCAGATGGAAGGTGTGAAGGATCATGAAGATGCTGACGTGAGTGATGAGGAAAAGAGTCTTATGAGAAAGTCTTCTGGAATTTAATTGGCTGTAGAGAAAC
ATGAGGCGATGAAGATTGGGCTGGTGATCCTTAGTAGGCCAAGTATTCGTTCTCTCTTAACATGATGCATAACCAATGACTGTCGTAAAGAAGACCAGgtacacattgcccgtgcatatttgtctatttcattattttgtcatcaaagcatccttggcgaaagaacttgccttggaattctgaaaagtggctgggttggt
ctttactcagcacttttttattatttatgtctgaagattattttgtgagcatgccagccttggcaattttccattgatgtattttggaggtagaagaagaaatcatttaaattaccatgggagctaagcaaaattgatgtttataattccattgaagtttaggtccatgtcattgatggcaatacttttaactttgtctc
atctttcagGGACCTGTAGGGTGTGAAGATGCATAAGTCTTATTTTCATTGGTTGCTATTTAACTGTTTCCTGATGTTGAGCTCCCACTGAGCATCTGTTTCTTGAATTTTAACTTGTGGATCTCTTACACATGAGAATGAGTCAAATGCAGCATGATGTTGTTTACTCACTTATATAAAGTAGCACTGTAATTTCAAAT
TTGTCTATTTTGTGTTGTGGAAAATAAGTAGAATGCAAACTTACGTTGCTTCTGTCTCTATGGCCACTCTCTATTGTTTCAAAGCTCTTAGAATTTAGATGTTGGATCAGAATGTTTCAGTTTAGTTGCCCTACACTGTTTGTAACCTAAAATTTGGGAAAAAATGCAATCATATGTTTGTTTTCAATAAATTGTGATCT
TGGAGGAGGC

Intron 2 GTACTACTTG ... AACGAAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.43	51.6	35	23
A2	46.25	39.6	33	31
A3	46.03	38.7	40	24
A4	63.23	11.4	26	11
A5	60.54	16.7	39	9
A6	47.61	95.4	99	59
A7	49.98	107.7	123	80
A8	42.18	70.3	59	37
A9	48.31	129.1	136	88
A10	44.05	148.7	137	78

>TRINITY_DN31108_c0_g1_i3 from sample A6 / Example spliced isoform

GCGGGTCAAGCTATTCACACCAGATTTGAAGTAAAAGCATCGAAAAACCCTCGGTAGAAGGTTTCCAGAGCTCGCATTGGATACCCACATCCAGATCTAGGAAAAAATGGCGGGTAGATCAGAATCTTCGAAGCAATACTATGCAACGGCCACCGTCATTGTAGGCTATGCTCTGTGCTCTAGTTTGCTTGCAGTTATAA
ACAAATATGCAATCACTAAGTTCAATTACCCAGGCTTGATGACTGCCCTACAGTACTTGACGAGCGTAATTGGTGTTTGGGTTTTTGGCAAATTGGGTTTGTTGCATCACGATCCATTTGTTTGGGAAACTGCTAAGAAATTCATGCCAGCTGCATTTGTTTTTTACTTGGCAATATTCACTAATACAAACCTTCTCAAG
CATGCCAATGTAGATACATTCATTGTGTTCAGATCTCTGACCCCACTTCTTGTAGCCATAGCTGATACTGCATTCAGGAAGCAGCCATGGCCTTCTAAGTATACTTTTATGAGCCTAGTGGTTATATTGGGGGGTGCAGTTGGGTATGTAGCAACTGATTCGGGCTTTACACTGACTGCATATTCATGGGCTTTTGCTTA
TCTGATCACCATTGTTACAGAGATGGTTTACATCAAACACATGGTTACAAATCTGGGTCTGAATACATGGGGGTTTGTGTTGTACAACAATTTGCTGTCTCTGATGCTGGCACCGGTGTTCTGGATCTTGACTGGGGAGTATGTGGAAGTGTTTTCTGCTGCAGCGGATGCTGGTATGAGAGGTCTGTTGATATATGATT
CCATATTTGCAGTGGGCCTGTCCTGTGTTTTTGGCCTTGCCATCAGCTTTTTTGGTTTTGCAGCGAGGAAGGCGGTGACAGCAACCGCATTCACTGTGACTGGGGTGGTGAACAAGTTCCTGACTGTGGTTATCAATGTGCTGATCTGGGACAAGCATGCCACTCCTGTTGGTCTTATCTTCCTCCTTATGACTATTGTG
GGTGGGGTGCTCTATCAGCAATCTGTGACCGTTGCTCCCGCCCCTCATATTGACAGCGGGATGAGTAAGCAGATGGAAGGTGTGAAGGATCATGAAGATGCTGACGTGAGTGATGAGGAAAAGAGTCTTATGAGAAAGTCTTCTGGAATTTAATTGGCTGTAGAGAAACATGAGGCGATGAAGATTGGGCTGGTGATCCT
TAGTAGGCCAAGTATTCGTTCTCTCTTAACATGATGCATAACCAATGACTGTCGTAAAGAAGACCAGGTACACATTGCCCGTGCATATTTGTCTATTTCATTATTTTGTCATCAAAGCATCCTTGGCGAAAGAACTTGCCTTGGAATTCTGAAAAGTGGCTGGGTTGGTCTTTACTCAGCACTTTTTTATTATTTATGTC
TGAAGATTATTTTGTGAGCATGCCAGCCTTGGCAATTTTCCATTGATGTATTTTGGAGGTAGAAGAAGAAATCATTTAAATTACCATGGGAGCTAAGCAAAATTGATGTTTATAATTCCATTGAAGTTTAGGTCCATGTCATTGATGGCAATACTTTTAACTTTGTCTCATCTTTCAGGGACCTGTAGGGTGTGAAGATG
CATAAGTCTTATTTTCATTGGTTGCTATTTAACTGTTTCCTGATGTTGAGCTCCCACTGAGCATCTGTTTCTTGAATTTTAACTTGTGGATCTCTTACACATGAGAATGAGTCAAATGCAGCATGATGTTGTTTACTCACTTATATAAAGTAGCACTGTAATTTCAAATTTGTCTATTTTGTGTTGTGGAAAATAAGTAG
AATGCAAACTTACGTTGCTTCTGTCTCTATGGCCACTCTCTATTGTTTCAAAGCTCTTAGAATTTAGATGTTGGATCAGAATGTTTCAGTTTAGTTGCCCTACACTGTTTGTAACCTAAAATTTGGGAAAAAATGCAATCATATGTTTGTTTTCAATAAATTGTGATCTTGGAGGAGGTTATAGCCACAAAACCAATGCT
GTGGTTATTTGAGGGAAATCCATGTAATATCAAATGTCAAGCTGCTTTCTTCATCAAATTTCAGTTTTGTGGGAAAGAAAATATGAATTGTTGGCATCAATTATTTTCTGCTTTTCAATAGTATAACCTTGTTTTGTGTTATTTCCATTTCAC

>TRINITY_DN31108_c0_g1_i2 from sample A6 / Example unspliced isoform:

GGAAAATTCCGACATATGCACAAGTGTGGACAAAATCACCGAACCTCATTGAAATAGTGGTAGGTTATCGTTTAAAGCGGGACACAATTTCCCAAACAAAGAATCGAGTCATCCTCTTATATATTCAGCCATTTTCATGCATTCGAGACAAACACAGTATGCATGACAAAGGTTTCAGCCATTCGAGACAAACGGAACAG
GACGAATGACAATAGTTTGGGCCATTTTCATTCATTTGACACAAATATGTAGCGAAAGTACTTAAAAAAGAGGTCAAACCCAGGATTTCTGCCAGCAAAGCAGGCGGGTCAAGCTATTCACACCAGgtactacttgtggttctcttcgcttttcatggctgattcatttttcttcacgtttatacatgcaatggatctca
aagagcttaaaatcccctcattgattgcgtgaagtctacgttcctaacgaagcagATTTGAAGTAAAAGCATCGAAAAACCCTCGGTAGAAGGTTTCCAGAGCTCGCATTGGATACCCACATCCAGATCTAGGAAAAAATGGCGGGTAGATCAGAATCTTCGAAGCAATACTATGCAACGGCCACCGTCATTGTAGGCTA
TGCTCTGTGCTCTAGTTTGCTTGCAGTTATAAACAAATATGCAATCACTAAGTTCAATTACCCAGGCTTGATGACTGCCCTACAGTACTTGACGAGCGTAATTGGTGTTTGGGTTTTTGGCAAATTGGGTTTGTTGCATCACGATCCATTTGTTTGGGAAACTGCTAAGAAATTCATGCCAGCTGCATTTGTTTTTTACT
TGGCAATATTCACTAATACAAACCTTCTCAAGCATGCCAATGTAGATACATTCATTGTGTTCAGATCTCTGACCCCACTTCTTGTAGCCATAGCTGATACTGCATTCAGGAAGCAGCCATGGCCTTCTAAGTATACTTTTATGAGCCTAGTGGTTATATTGGGGGGTGCAGTTGGGTATGTAGCAACTGATTCGGGCTTT
ACACTGACTGCATATTCATGGGCTTTTGCTTATCTGATCACCATTGTTACAGAGATGGTTTACATCAAACACATGGTTACAAATCTGGGTCTGAATACATGGGGGTTTGTGTTGTACAACAATTTGCTGTCTCTGATGCTGGCACCGGTGTTCTGGATCTTGACTGGGGAGTATGTGGAAGTGTTTTCTGCTGCAGCGGA
TGCTGGTATGAGAGGTCTGTTGATATATGATTCCATATTTGCAGTGGGCCTGTCCTGTGTTTTTGGCCTTGCCATCAGCTTTTTTGGTTTTGCAGCGAGGAAGGCGGTGACAGCAACCGCATTCACTGTGACTGGGGTGGTGAACAAGTTCCTGACTGTGGTTATCAATGTGCTGATCTGGGACAAGCATGCCACTCCTG
TTGGTCTTATCTTCCTCCTTATGACTATTGTGGGTGGGGTGCTCTATCAGCAATCTGTGACCGTTGCTCCCGCCCCTCATATTGACAGCGGGATGAGTAAGCAGATGGAAGGTGTGAAGGATCATGAAGATGCTGACGTGAGTGATGAGGAAAAGAGTCTTATGAGAAAGTCTTCTGGAATTTAATTGGCTGTAGAGAAA
CATGAGGCGATGAAGATTGGGCTGGTGATCCTTAGTAGGCCAAGTATTCGTTCTCTCTTAACATGATGCATAACCAATGACTGTCGTAAAGAAGACCAGGTACACATTGCCCGTGCATATTTGTCTATTTCATTATTTTGTCATCAAAGCATCCTTGGCGAAAGAACTTGCCTTGGAATTCTGAAAAGTGGCTGGGTTGG
TCTTTACTCAGCACTTTTTTATTATTTATGTCTGAAGATTATTTTGTGAGCATGCCAGCCTTGGCAATTTTCCATTGATGTATTTTGGAGGTAGAAGAAGAAATCATTTAAATTACCATGGGAGCTAAGCAAAATTGATGTTTATAATTCCATTGAAGTTTAGGTCCATGTCATTGATGGCAATACTTTTAACTTTGTCT
CATCTTTCAGGGACCTGTAGGGTGTGAAGATGCATAAGTCTTATTTTCATTGGTTGCTATTTAACTGTTTCCTGATGTTGAGCTCCCACTGAGCATCTGTTTCTTGAATTTTAACTTGTGGATCTCTTACACATGAGAATGAGTCAAATGCAGCATGATGTTGTTTACTCACTTATATAAAGTAGCACTGTAATTTCAAA
TTTGTCTATTTTGTGTTGTGGAAAATAAGTAGAATGCAAACTTACGTTGCTTCTGTCTCTATGGCCACTCTCTATTGTTTCAAAGCTCTTAGAATTTAGATGTTGGATCAGAATGTTTCAGTTTAGTTGCCCTACACTGTTTGTAACCTAAAATTTGGGAAAAAATGCAATCATATGTTTGTTTTCAATAAATTGTGATC
TTGGAGGAGGTTATAGCCACAAAACCAATGCTGTGGTTATTTGAGGGAAATCCATGTAATATCAAATGTCAAGCTGCTTTCTTCATCAAATTTCAGTTTTGTGGGAAAGAAAATATGAATTGTTGGCATCAATTATTTTCTGCTTTTCAATAGTATAACCTTGTTTTGTGTTATTTCCATTTCAC

Intron 3 GTACTACTTG ... TTGCGTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.43	60	35	20
A2	45	33	33	21
A3	46.34	33	40	17
A4	57.58	14	26	12
A5	56.52	20	39	13
A6	44.05	94	99	49
A7	46.54	112	123	72
A8	37.99	71	59	28
A9	44.49	136	136	82
A10	39.84	148	137	59

>TRINITY_DN103441_c0_g1_i2 from sample A1 / Example spliced isoform

TACACAGGAAAATTCCGACATATGCACAAGTGTGGACAAAATCACCGAACCTCATTGAAATAGTGGTAGGTTATCGTTTAAAGCGGGACACAATTTCCCAAACAAAGAATCGAGTCATCCTCTTATATATTCAGCCATTTTCATGCATTCGAGACAAACACAGTATGCATGACAAAGGTTTCAGCCATTCGAGACAAACG
GAACAGGACGAATGACAATAGTTTGGGCCATTTTCATTCATTTGACACAAATATGTAGCGAAAGTACTTAAAAAAGAGGTCAAACCCAGGATTTCTGCCAGCAAAGCAGGCGGGTCAAGCTATTCACACCAGTCTACGTTCCTAACGAAGCAGATTTGAAGTAAAAGCATCGAAAAACCCTCGGTAGAAGGTTTCCAGAG
CTCGCATTGGATACCCACATCCAGATCTAGGAAAAAATGGCGGGTAGATCAGAATCTTCGAAGCAATACTATGCAACGGCCACCGTCATTGTAGGCTATGCTCTGTGCTCTAGTTTGCTTGCAGTTATAAACAAATATGCAATCACTAAGTTCAATTACCCAGGCTTGATGACTGCCCTACAGTACTTGACGAGCGTAAT
TGGTGTTTGGGTTTTTGGCAAATTGGGTTTGTTGCATCACGATCCATTTGTTTGGGAAACTGCTAAGAAATTCATGCCAGCTGCATTTGTTTTTTACTTGGCAATATTCACTAATACAAACCTTCTCAAGCATGCCAATGTAGATACATTCATTGTGTTCAGATCTCTGACCCCACTTCTTGTAGCCATAGCTGATACTG
CATTCAGGAAGCAGCCATGGCCTTCTAAGTATACTTTTATGAGCCTAGTGGTTATATTGGGGGGTGCAGTTGGGTATGTAGCAACTGATTCGGGCTTTACACTGACTGCATATTCATGGGCTTTTGCTTATCTGATCACCATTGTTACAGAGATGGTTTACATCAAACACATGGTTACAAATCTGGGTCTGAATACATGG
GGGTTTGTGTTGTACAACAATTTGCTGTCTCTGATGCTGGCACCGGTGTTCTGGATCTTGACTGGGGAGTATGTGGAAGTGTTTTCTGCTGCAGCGGATGCTGGTATGAGAGGTCTGTTGATATATGATTCCATATTTGCAGTGGGCCTGTCCTGTGTTTTTGGCCTTGCCATCAGCTTTTTTGGTTTTGCAGCGAGGAA
GGCGGTGACAGCAACCGCATTCACTGTGACTGGGGTGGTGAACAAGTTCCTGACTGTGGTTATCAATGTGCTGATCTGGGACAAGCATGCCACTCCTGTTGGTCTTATCTTCCTCCTTATGACTATTGTGGGTGGGGTGCTCTATCAGCAATCTGTGACCGTTGCTCCCGCCCCTCATATTGACAGCGGGATGAGTAAGC
AGATGGAAGGTGTGAAGGATCATGAAGATGCTGACGTGAGTGATGAGGAAAAGAGTCTTATGAGAAAGTCTTCTGGAATTTAATTGGCTGTAGAGAAACATGAGGCGATGAAGATTGGGCTGGTGATCCTTAGTAGGCCAAGTATTCGTTCTCTCTTAACATGATGCATAACCAATGACTGTCGTAAAGAAGACCAGGTA
CACATTGCCCGTGCATATTTGTCTATTTCATTATTTTGTCATCAAAGCATCCTTGGCGAAAGAACTTGCCTTGGAATTCTGAAAAGTGGCTGGGTTGGTCTTTACTCAGCACTTTTTTATTATTTATGTCTGAAGATTATTTTGTGAGCATGCCAGCCTTGGCAATTTTCCATTGATGTATTTTGGAGGTAGAAGAAGAA
ATCATTTAAATTACCATGGGAGCTAAGCAAAATTGATGTTTATAATTCCATTGAAGTTTAGGTCCATGTCATTGATGGCAATACTTTTAACTTTGTCTCATCTTTCAGGGACCTGTAGGGTGTGAAGATGCATAAGTCTTATTTTCATTGGTTGCTATTTAACTGTTTCCTGATGTTGAGCTCCCACTGAGCATCTGTTT
CTTGAATTTTAACTTGTGGATCTCTTACACATGAGAATGAGTCAAATGCAGCATGATGTTGTTTACTCACTTATATAAAGTAGCACTGTAATTTCAAATTTGTCTATTTTGTGTTGTGGAAAATAAGTAGAATGCAAACTTACGTTGCTTCTGTCTCTATGGCCACTCTCTATTGTTTCAAAGCTCTTAGAATTTAGATG
TTGGATCAGAATGTTTCAGTTTAGTTGCCCTACACTGTTTGTAACCTAAAATTTGGGAAAAAATGCAATCATATGTTTGTTTTCAATAAATTGTGATCTTGGAGGAGGTTATAGCCACAAAACCAATGCTGTGGTTATTTGAGGGAAATCCATGTAATATCAAATGTCAAGCTGCTTTCTTCATCAAATTTCAGTTTTGT
GGGAAAGAAAATATGAATTGTTG

>TRINITY_DN103441_c0_g1_i1 from sample A1 / Example unspliced isoform:

TACACAGGAAAATTCCGACATATGCACAAGTGTGGACAAAATCACCGAACCTCATTGAAATAGTGGTAGGTTATCGTTTAAAGCGGGACACAATTTCCCAAACAAAGAATCGAGTCATCCTCTTATATATTCAGCCATTTTCATGCATTCGAGACAAACACAGTATGCATGACAAAGGTTTCAGCCATTCGAGACAAACG
GAACAGGACGAATGACAATAGTTTGGGCCATTTTCATTCATTTGACACAAATATGTAGCGAAAGTACTTAAAAAAGAGGTCAAACCCAGGATTTCTGCCAGCAAAGCAGGCGGGTCAAGCTATTCACACCAGgtactacttgtggttctcttcgcttttcatggctgattcatttttcttcacgtttatacatgcaatgg
atctcaaagagcttaaaatcccctcattgattgcgtgaagTCTACGTTCCTAACGAAGCAGATTTGAAGTAAAAGCATCGAAAAACCCTCGGTAGAAGGTTTCCAGAGCTCGCATTGGATACCCACATCCAGATCTAGGAAAAAATGGCGGGTAGATCAGAATCTTCGAAGCAATACTATGCAACGGCCACCGTCATTGT
AGGCTATGCTCTGTGCTCTAGTTTGCTTGCAGTTATAAACAAATATGCAATCACTAAGTTCAATTACCCAGGCTTGATGACTGCCCTACAGTACTTGACGAGCGTAATTGGTGTTTGGGTTTTTGGCAAATTGGGTTTGTTGCATCACGATCCATTTGTTTGGGAAACTGCTAAGAAATTCATGCCAGCTGCATTTGTTT
TTTACTTGGCAATATTCACTAATACAAACCTTCTCAAGCATGCCAATGTAGATACATTCATTGTGTTCAGATCTCTGACCCCACTTCTTGTAGCCATAGCTGATACTGCATTCAGGAAGCAGCCATGGCCTTCTAAGTATACTTTTATGAGCCTAGTGGTTATATTGGGGGGTGCAGTTGGGTATGTAGCAACTGATTCG
GGCTTTACACTGACTGCATATTCATGGGCTTTTGCTTATCTGATCACCATTGTTACAGAGATGGTTTACATCAAACACATGGTTACAAATCTGGGTCTGAATACATGGGGGTTTGTGTTGTACAACAATTTGCTGTCTCTGATGCTGGCACCGGTGTTCTGGATCTTGACTGGGGAGTATGTGGAAGTGTTTTCTGCTGC
AGCGGATGCTGGTATGAGAGGTCTGTTGATATATGATTCCATATTTGCAGTGGGCCTGTCCTGTGTTTTTGGCCTTGCCATCAGCTTTTTTGGTTTTGCAGCGAGGAAGGCGGTGACAGCAACCGCATTCACTGTGACTGGGGTGGTGAACAAGTTCCTGACTGTGGTTATCAATGTGCTGATCTGGGACAAGCATGCCA
CTCCTGTTGGTCTTATCTTCCTCCTTATGACTATTGTGGGTGGGGTGCTCTATCAGCAATCTGTGACCGTTGCTCCCGCCCCTCATATTGACAGCGGGATGAGTAAGCAGATGGAAGGTGTGAAGGATCATGAAGATGCTGACGTGAGTGATGAGGAAAAGAGTCTTATGAGAAAGTCTTCTGGAATTTAATTGGCTGTA
GAGAAACATGAGGCGATGAAGATTGGGCTGGTGATCCTTAGTAGGCCAAGTATTCGTTCTCTCTTAACATGATGCATAACCAATGACTGTCGTAAAGAAGACCAGGTACACATTGCCCGTGCATATTTGTCTATTTCATTATTTTGTCATCAAAGCATCCTTGGCGAAAGAACTTGCCTTGGAATTCTGAAAAGTGGCTG
GGTTGGTCTTTACTCAGCACTTTTTTATTATTTATGTCTGAAGATTATTTTGTGAGCATGCCAGCCTTGGCAATTTTCCATTGATGTATTTTGGAGGTAGAAGAAGAAATCATTTAAATTACCATGGGAGCTAAGCAAAATTGATGTTTATAATTCCATTGAAGTTTAGGTCCATGTCATTGATGGCAATACTTTTAACT
TTGTCTCATCTTTCAGGGACCTGTAGGGTGTGAAGATGCATAAGTCTTATTTTCATTGGTTGCTATTTAACTGTTTCCTGATGTTGAGCTCCCACTGAGCATCTGTTTCTTGAATTTTAACTTGTGGATCTCTTACACATGAGAATGAGTCAAATGCAGCATGATGTTGTTTACTCACTTATATAAAGTAGCACTGTAAT
TTCAAATTTGTCTATTTTGTGTTGTGGAAAATAAGTAGAATGCAAACTTACGTTGCTTCTGTCTCTATGGCCACTCTCTATTGTTTCAAAGCTCTTAGAATTTAGATGTTGGATCAGAATGTTTCAGTTTAGTTGCCCTACACTGTTTGTAACCTAAAATTTGGGAAAAAATGCAATCATATGTTTGTTTTCAATAAATT
GTGATCTTGGAGGAGGTTATAGCCACAAAACCAATGCTGTGGTTATTTGAGGGAAATCCATGTAATATCAAATGTCAAGCTGCTTTCTTCATCAAATTTCAGTTTTGTGGGAAAGAAAATATGAATTGTTG

Intron 4 GTCTGTTGAT ... GGTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	81.05	9	41	36
A2	79.78	9	31	40
A3	73.08	14	37	39
A4	70.54	19	48	43
A5	54.17	11	16	10
A6	80.67	92	365	403
A7	82.75	74	341	369
A8	76.09	85	243	298
A9	83	76	354	388
A10	78.62	85	298	327

>TRINITY_DN98749_c0_g1_i4 from sample A5 / Example spliced isoform

GAAAATTCCGACATATGCACAAGTGTGGACAAAATCACCGAACCTCATTGAAATAGTGGTAGGTTATCGTTTAAAGCGGGACACAATTTCCCAAACAAAGAATCGAGTCATCCTCTTATATATTCAGCCATTTTCATGCATTCGAGACAAACACAGTATGCATGACAAAGGTTTCAGCCATTCGAGACAAACGGAACAGG
ACGAATGACAATAGTTTGGGCCATTTTCATTCATTTGACACAAATATGTAGCGAAAGTACTTAAAAAAGAGGTCAAACCCAGGATTTCTGCCAGCAAAGCAGGCGGGTCAAGCTATTCACACCAGGTACTACTTGTGGTTCTCTTCGCTTTTCATGGCTGATTCATTTTTCTTCACGTTTATACATGCAATGGATCTCAA
AGAGCTTAAAATCCCCTCATTGATTGCGTGAAGTCTACGTTCCTAACGAAGCAGATTTGAAGTAAAAGCATCGAAAAACCCTCGGTAGAAGGTTTCCAGAGCTCGCATTGGATACCCACATCCAGATCTAGGAAAAAATGGCGGGTAGATCAGAATCTTCGAAGCAATACTATGCAACGGCCACCGTCATTGTAGGCTAT
GCTCTGTGCTCTAGTTTGCTTGCAGTTATAAACAAATATGCAATCACTAAGTTCAATTACCCAGGCTTGATGACTGCCCTACAGTACTTGACGAGCGTAATTGGTGTTTGGGTTTTTGGCAAATTGGGTTTGTTGCATCACGATCCATTTGTTTGGGAAACTGCTAAGAAATTCATGCCAGCTGCATTTGTTTTTTACTT
GGCAATATTCACTAATACAAACCTTCTCAAGCATGCCAATGTAGATACATTCATTGTGTTCAGATCTCTGACCCCACTTCTTGTAGCCATAGCTGATACTGCATTCAGGAAGCAGCCATGGCCTTCTAAGTATACTTTTATGAGCCTAGTGGTTATATTGGGGGGTGCAGTTGGGTATGTAGCAACTGATTCGGGCTTTA
CACTGACTGCATATTCATGGGCTTTTGCTTATCTGATCACCATTGTTACAGAGATGGTTTACATCAAACACATGGTTACAAATCTGGGTCTGAATACATGGGGGTTTGTGTTGTACAACAATTTGCTGTCTCTGATGCTGGCACCGGTGTTCTGGATCTTGACTGGGGAGTATGTGGAAGTGTTTTCTGCTGCAGCGGAT
GCTGGTATGAGAGCGAGGAAGGCGGTGACAGCAACCGCATTCACTGTGACTGGGGTGGTGAACAAGTTCCTGACTGTGGTTATCAATGTGCTGATCTGGGACAAGCATGCCACTCCTGTTGGTCTTATCTTCCTCCTTATGACTATTGTGGGTGGGGTGCTCTATCAGCAATCTGTGACCGTTGCTCCCGCCCCTCATAT
TGACAGCGGGATGAGTAAGCAGATGGAAGGTGTGAAGGATCATGAAGATGCTGACGTGAGTGATGAGGAAAAGAGTCTTATGAGAAAGTCTTCTGGAATTTAATTGGCTGTAGAGAAACATGAGGCGATGAAGATTGGGCTGGTGATCCTTAGTAGGCCAAGTATTCGTTCTCTCTTAACATGATGCATAACCAATGACT
GTCGTAAAGAAGACCAGGTACACATTGCCCGTGCATATTTGTCTATTTCATTATTTTGTCATCAAAGCATCCTTGGCGAAAGAACTTGCCTTGGAATTCTGAAAAGTGGCTGGGTTGGTCTTTACTCAGCACTTTTTTATTATTTATGTCTGAAGATTATTTTGTGAGCATGCCAGCCTTGGCAATTTTCCATTGATGTA
TTTTGGAGGTAGAAGAAGAAATCATTTAAATTACCATGGGAGCTAAGCAAAATTGATGTTTATAATTCCATTGAAGTTTAGGTCCATGTCATTGATGGCAATACTTTTAACTTTGTCTCATCTTTCAGGGACCTGTAGGGTGTGAAGATGCATAAGTCTTATTTTCATTGGTTGCTATTTAACTGTTTCCTGATGTTGAG
CTCCCACTGAGCATCTGTTTCTTGAATTTTAACTTGTGGATCTCTTACACATGAGAATGAGTCAAATGCAGCATGATGTTGTTTACTCACTTATATAAAGTAGCACTGTAATTTCAAATTTGTCTATTTTGTGTTGTGGAAAATAAGTAGAATGCAAAC

>TRINITY_DN98749_c0_g1_i3 from sample A5 / Example unspliced isoform:

GAAAATTCCGACATATGCACAAGTGTGGACAAAATCACCGAACCTCATTGAAATAGTGGTAGGTTATCGTTTAAAGCGGGACACAATTTCCCAAACAAAGAATCGAGTCATCCTCTTATATATTCAGCCATTTTCATGCATTCGAGACAAACACAGTATGCATGACAAAGGTTTCAGCCATTCGAGACAAACGGAACAGG
ACGAATGACAATAGTTTGGGCCATTTTCATTCATTTGACACAAATATGTAGCGAAAGTACTTAAAAAAGAGGTCAAACCCAGGATTTCTGCCAGCAAAGCAGGCGGGTCAAGCTATTCACACCAGTCTACGTTCCTAACGAAGCAGATTTGAAGTAAAAGCATCGAAAAACCCTCGGTAGAAGGTTTCCAGAGCTCGCAT
TGGATACCCACATCCAGATCTAGGAAAAAATGGCGGGTAGATCAGAATCTTCGAAGCAATACTATGCAACGGCCACCGTCATTGTAGGCTATGCTCTGTGCTCTAGTTTGCTTGCAGTTATAAACAAATATGCAATCACTAAGTTCAATTACCCAGGCTTGATGACTGCCCTACAGTACTTGACGAGCGTAATTGGTGTT
TGGGTTTTTGGCAAATTGGGTTTGTTGCATCACGATCCATTTGTTTGGGAAACTGCTAAGAAATTCATGCCAGCTGCATTTGTTTTTTACTTGGCAATATTCACTAATACAAACCTTCTCAAGCATGCCAATGTAGATACATTCATTGTGTTCAGATCTCTGACCCCACTTCTTGTAGCCATAGCTGATACTGCATTCAG
GAAGCAGCCATGGCCTTCTAAGTATACTTTTATGAGCCTAGTGGTTATATTGGGGGGTGCAGTTGGGTATGTAGCAACTGATTCGGGCTTTACACTGACTGCATATTCATGGGCTTTTGCTTATCTGATCACCATTGTTACAGAGATGGTTTACATCAAACACATGGTTACAAATCTGGGTCTGAATACATGGGGGTTTG
TGTTGTACAACAATTTGCTGTCTCTGATGCTGGCACCGGTGTTCTGGATCTTGACTGGGGAGTATGTGGAAGTGTTTTCTGCTGCAGCGGATGCTGGTATGAGAGgtctgttgatatatgattccatatttgcagtgggcctgtcctgtgtttttggccttgccatcagcttttttggttttgcagCGAGGAAGGCGGTG
ACAGCAACCGCATTCACTGTGACTGGGGTGGTGAACAAGTTCCTGACTGTGGTTATCAATGTGCTGATCTGGGACAAGCATGCCACTCCTGTTGGTCTTATCTTCCTCCTTATGACTATTGTGGGTGGGGTGCTCTATCAGCAATCTGTGACCGTTGCTCCCGCCCCTCATATTGACAGCGGGATGAGTAAGCAGATGGA
AGGTGTGAAGGATCATGAAGATGCTGACGTGAGTGATGAGGAAAAGAGTCTTATGAGAAAGTCTTCTGGAATTTAATTGGCTGTAGAGAAACATGAGGCGATGAAGATTGGGCTGGTGATCCTTAGTAGGCCAAGTATTCGTTCTCTCTTAACATGATGCATAACCAATGACTGTCGTAAAGAAGACCAGGTACACATTG
CCCGTGCATATTTGTCTATTTCATTATTTTGTCATCAAAGCATCCTTGGCGAAAGAACTTGCCTTGGAATTCTGAAAAGTGGCTGGGTTGGTCTTTACTCAGCACTTTTTTATTATTTATGTCTGAAGATTATTTTGTGAGCATGCCAGCCTTGGCAATTTTCCATTGATGTATTTTGGAGGTAGAAGAAGAAATCATTT
AAATTACCATGGGAGCTAAGCAAAATTGATGTTTATAATTCCATTGAAGTTTAGGTCCATGTCATTGATGGCAATACTTTTAACTTTGTCTCATCTTTCAGGGACCTGTAGGGTGTGAAGATGCATAAGTCTTATTTTCATTGGTTGCTATTTAACTGTTTCCTGATGTTGAGCTCCCACTGAGCATCTGTTTCTTGAAT
TTTAACTTGTGGATCTCTTACACATGAGAATGAGTCAAATGCAGCATGATGTTGTTTACTCACTTATATAAAGTAGCACTGTAATTTCAAATTTGTCTATTTTGTGTTGTGGAAAATAAGTAGAATGCAAAC