sp|Q6XAT2|ERL2_ARATH ----> LRR receptor-like serine/threonine-protein kinase ERL2

Intron 1 GTAAGTTCCT ... TCACATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.42	8.2	15	11
A2	41.85	11.9	7	10
A3	50.45	6.5	6	7
A4	62.49	3.9	4	9
A5	60	1	0	3
A6	24.84	12.2	2	6
A7	21.2	33.5	3	15
A8	35.32	33.1	20	16
A9	25.44	31.5	6	15.4
A10	27.03	26.4	5	14

>TRINITY_DN120199_c0_g2_i3 from sample A2 / Example spliced isoform

CCTCAACCCTTCGGGAATGACAAATGTCTGCCCCTAAATAAAATATTTTGAAATTCATCAATAGAAGTGAGGTTTCAGAGCTCTGCCGTAAATAAACCTCATAATCCCTACTGATTCATTCAGCGATAAGTCGCATGATCCATGATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCGACCAGTTGATAATTCTTAGA
ATCCAGGTAGAAGGCTTTAAAAGGAGAGTCTTATGCTGGGGAGGTTTTATGTTGTCAATTTTAATCCTTAGACCTTTCTGCTAACTGATTATCGGATAATGCTCCTCAAGAACTTGTACAGCTATAGAATTTGGTCTCAGTGATACTGGATAACAACAATTTATCAGGGGAGGTGCCATATCAGTTGATCAACTGCTTCA
ACCTTGCTACACTAAATATCTCCTACAGCAATTTGTCTGGGAATATTCCTGTCATAAAGAACTTCACAAGATTTACCCCAGAAAGTGATAGGAAAAGTGGGAAGTACAAGTTAATGTAACATGTTATAGTGGTGACTGGAGTCCCTGAATATATACGAGGCAGCAACTAGCTCAGAAACTCGTGCACTTTTGGAGGAAAT
AAATTGCCAAACATAAGAATGTTGGATAAAACATGTTTCTTATCAAATACAATGAAATACTCATCGAAATGTGGATAAAACATGTTTCTTATCAAATACAATGAAATACTCATCGAAATGTGGATAATGAAACTAAAAAAAAAAACCACCAGCTGTATAAGTGGTTTTATGCATCTACTATAAGCAATTGTGAGATGTGG
GAGGAGAATATATAATGTGGGATTCACATTAAATATTATGAATTGAATGAATGCGGCACTACCTAGGGATAATCGAGAACCAATGGCAGCATCACCTACAGCCCCACGATCAGTGCCAATGGTAACATCATCTGATACATCATTGGCACCTCATTGATGGTCTTTATCGTGTCTCCATTTGGTTGTTTTTCGGATTGTAT
ATCTGAC

>TRINITY_DN120199_c0_g2_i4 from sample A2 / Example unspliced isoform:

GGATTTTCTTTTTCCTTTTCTCTAAAACTTTCAAAGCCTCTAGTTCTGATGCACTAAAAACTTTGAAATGTTCTGATAAAAAAGTGGCATAATCTCCAACTCTACCTTTTGCAATTCTTCTTGCCTTTCTTCCTTTTTTTGTTTGCCCCGAGCAACAACTAATTGATTCACCCGTTTGATATTGGAAAACTACATCATCC
TTGAATTGTTGTCCTGTGGATCGTATAACTTCCTCTTCCCCAAGATAAGAGCCTGAAAATCCTTCTCAACTAGCCATGCATAATTGAACTTAAAAAGACCAGGGACTTCTTATCTTCTTTGTCTAATTGTAACAAAATTAGACAGTGATCAAAAGAACCTCCCAAACCTACCCATGACCTATAGTGATCAACCACCCCCA
CAAACTTCTCTGCCATGAAGAATCTATGCAGCCGTTTTGAGATGCTCAACCTACCTCCCCTCGCATTTTGTCAAGTCGGAATTAATTTGGTATCTTCAATATCAATGAGCTTCGCATCATCAAAGAGGTGGATGAAATATTCAACCAAAAGATCCTGCCTAGGCAACTCTTCAAATTTCTCGGGGTTACAATGTTATATT
AAATCACCACCCAGAATTACATTTTTTAAAATTGTAAGCTTTGTCTGTAGAAATCTTTTGAGAACAAACCAAGTTTTTATGGGTGAGAGTCATATTAATCATCATGGTCTCCATGGCCACTTCAAGAAAACAGTTACATTGTGTGACATATTTTCAATGCAGAGACCTTTCTGCTAACTGATTATCGGATAATGCTCCTC
AAGAACTTGTACAGCTATAGAATTTGGTCTCAGTgtaagttccttggaacgatccctaaaatacaatttatttaataggctcacaattattctgaaaataattctggagatggatcacatacagGATACTGGATAACAACAATTTATCAGGGGAGGTGCCATATCAGTTGATCAACTGCTTCAACCTTGCTACACTAAAT
ATCTCCTACAGCAATTTGTCTGGGAATATTCCTGTCATAAAGAACTTCACAAGATTTACCCCAGAAAGTGATAGGAAAAGTGGGAAGTACAAGTTAATGTAACATGTTATAGTGGTGACTGGAGTCCCTGAATATATACGAGGCAGCAACTAGCTCAGAAACTCGTGCACTTTTGGAGGAAATAAATTGCCAAACATAAG
AATGTTGGATAAAACATGTTTCTTATCAAATACAATGAAATACTCATCGAAATGTGGATAAAACATGTTTCTTATCAAATACAATGAAATACTCATCGAAATGTGGATAATGAAACTAAAAAAAAAAACCACCAGCTGTATAAGTGGTTTTATGCATCTACTATAAGCAATTGTGAGATGTGGGAGGAGAATATATAATG
TGGGATTCACATTAAATATTATGAATTGAATGAATGCGGCACTACCTAGGGATAATCGAGAACCAATGGCAGCATCACCTACAGCCCCACGATCAGTGCCAATGGTAACATCATCTGATACATCATTGGCACCTCATTGATGGTCTTTATCGTGTCTCCATTTGGTTGTTTTTCGGATTGTATATCTGAC

Intron 2 GTAGAAGGCT ... CCCCAGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	53.41	9.8	11	11.5
A2	59.13	5	8	6.5
A3	47.49	12.5	9	13.3
A4	75.45	1.8	5	5.5
A5	44.09	5.3	3	5.5
A6	54.51	9.3	14	8
A7	54.19	12.5	10	19.3
A8	73	6.8	6	21.5
A9	62.73	3.8	5	7.8
A10	48.87	13.8	8	16

>TRINITY_DN107813_c0_g1_i4 from sample A3 / Example spliced isoform

TTAGAGCTCTGCCGTAAATAAACCTCATAATCCCTACTGATTCATTCAGCGATAAGTCGCATGATCCATGATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCGACCAGTTGATAATTCTTAGAATCCAGTGATAGGAAAAGTGGGAAGTACAAGTTAATGTAACATGTTATAGTGGTGACTGGAGTCCCTGAATATA
TACGAGGCAGCAACTAGCTCAGAAACTCGTGCACTTTTGGAGGAAATAAATTGCCAAACATAAGAATGTTGGATAAAACATGTTTCTTATCAAATACAATGAAATACTCATCGAAATGTGGATAATGAAACTAAAAAAAAAAACCACCAGCTGTATAAGTGGTTTTATGCATCTACTATAAGCAATTGTGAGATGTGGGA
GGAGAATATATAATGTGGGATTCACATTAAATATTATGAATTGAATGAATGCGGCACTACCTAGGGATAATCGAGAACCAATGGCAGCATCACCTACAGCCCCACGATCAGTGCCAATGGTAACATCATCTGATACATCATTGGCACCTCATTGATGGTCTTTATCGTGTCTCCATTTGGTTGTTTTTCGGATTGTATAT
CTGACATCAGGGACAACTTCTCTGATTTTGGATTGTATATCTGACATCAGGGACAACTTCTGTGTTTTGGCAGAACAAATGGTCTAATGTATTTTGTATTGAGATCTAAGTTCTGTACGGCAATATAATGCGTATGGGGCTATGGGATGAAGTAGTTTGCATGGACCAAAGAGCAGGGGACTTGAGATATTTTGCAATTA
ATTTTTATGGGCAAAGCCCACTATTTCTGATTTTTCTTAAGGTTTGGCCTGCAAAGGGTTTTTAATTTCTT

>TRINITY_DN107813_c0_g1_i5 from sample A3 / Example unspliced isoform:

TTAGAGCTCTGCCGTAAATAAACCTCATAATCCCTACTGATTCATTCAGCGATAAGTCGCATGATCCATGATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCGACCAGTTGATAATTCTTAGAATCCAGgtagaaggctttaaaaggagagtcttatgctggggaggttttatgttgtcaattttaatccttagacc
tttctgctaactgattatcggataatgctcctcaagaacttgtacagctatagaatttggtctcagtgatactggataacaacaatttatcaggggaggtgccatatcagttgatcaactgcttcaaccttgctacactaaatatctcctacagcaatttgtctgggaatattcctgtcataaagaacttcacaagattt
accccagaaagTGATAGGAAAAGTGGGAAGTACAAGTTAATGTAACATGTTATAGTGGTGACTGGAGTCCCTGAATATATACGAGGCAGCAACTAGCTCAGAAACTCGTGCACTTTTGGAGGAAATAAATTGCCAAACATAAGAATGTTGGATAAAACATGTTTCTTATCAAATACAATGAAATACTCATCGAAATGTGG
ATAATGAAACTAAAAAAAAAAACCACCAGCTGTATAAGTGGTTTTATGCATCTACTATAAGCAATTGTGAGATGTGGGAGGAGAATATATAATGTGGGATTCACATTAAATATTATGAATTGAATGAATGCGGCACTACCTAGGGATAATCGAGAACCAATGGCAGCATCACCTACAGCCCCACGATCAGTGCCAATGGT
AACATCATCTGATACATCATTGGCACCTCATTGATGGTCTTTATCGTGTCTCCATTTGGTTGTTTTTCGGATTGTATATCTGACATCAGGGACAACTTCTCTGATTTTGGATTGTATATCTGACATCAGGGACAACTTCTGTGTTTTGGCAGAACAAATGGTCTAATGTATTTTGTATTGAGATCTAAGTTCTGTACGGC
AATATAATGCGTATGGGGCTATGGGATGAAGTAGTTTGCATGGACCAAAGAGCAGGGGACTTGAGATATTTTGCAATTAATTTTTATGGGCAAAGCCCACTATTTCTGATTTTTCTTAAGGTTTGGCCTGCAAAGGGTTTTTAATTTCTT

Intron 3 GTAGAAGGCT ... CCCCAGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.38	10	9	13
A2	60	4	4	8
A3	46.15	14	9	15
A4	71.43	2	4	6
A5	45.45	6	3	7
A6	45.95	10	8	9
A7	50.88	14	7	22
A8	60.87	9	2	26
A9	63.64	4	5	9
A10	40.35	17	3	20

>TRINITY_DN42153_c1_g1_i10 from sample A9 / Example spliced isoform

CAAATGTCTGCCCCTAAATAAAATATTTTGAAATTCATCAATAGAAGTGAGGTTTCAGAGCTCTGCCGTAAATAAACCTCATAATCCCTACTGATTCATTCAGCGATAAGTCGCATGATCCATGATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCGACCAGTTGATAATTCTTAGAATCCAGTGATAGGAAAAGTG
GGAAGTACAAGTTAATGTAACATGTTATAGTGGTGACTGGAGTCCCTGAATATATACGAGGCAGCAACTAGCTCAGAAACTCGTGCACTTTTGGAGGAAATAAATTGCCAAACATAAGAATGTTGGATAAAACATGTTTCTTATCAAATACAATGAAATACTCATCGAAATGTGG

>TRINITY_DN42153_c1_g1_i20 from sample A9 / Example unspliced isoform:

CAAATGTCTGCCCCTAAATAAAATATTTTGAAATTCATCAATAGAAGTGAGGTTTCAGAGCTCTGCCGTAAATAAACCTCATAATCCCTACTGATTCATTCAGCGATAAGTCGCATGATCCATGATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCGACCAGTTGATAATTCTTAGAATCCAGgtagaaggctttaa
aaggagagtcttatgctggggaggttttatgttgtcaattttaatccttgatactgaataacaacaatttatcaggggaggtgccatatcagttgatcaactgcttcaaccttgctacactaaatatctcctacagcaatttgtctgggaatattcctgtcataaagaacttcacaagatttaccccagaaagTGATAGG
AAAAGTGGGAAGTACAAGTTAATGTAACATGTTATAGTGGTGACTGGAGTCCCTGAATATATACGAGGCAGCAACTAGCTCAGAAACTCGTGCACTTTTGGAGGAAATAAATTGCCAAACATAAGAATGTTGGATAAAACATGTTTCTTATCAAATACAATGAAATACTCATCGAAATGTGG

Intron 4 GTTAGCGTTA ... TTTTAACTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.2	15	62.3	53
A2	83.91	10	62.7	42
A3	63.77	20	33.7	37
A4	63.69	16	36	21
A5	100	0	28	20
A6	54.43	34	36.7	44.7
A7	56.76	47	54.7	69
A8	71.51	16	33.3	47
A9	50.77	36	19.3	55
A10	47.41	51	38	54

>TRINITY_DN107813_c0_g1_i16 from sample A3 / Example spliced isoform

AGGGATTTCCACCGCAGCCGATTCGCGTTGCAGGGCTTTCGACAGATTATATTTGTTAGATGGTTTTTTCGTCTCAGGGTTTTCAAATGATCGAGGCTTTAGGGTTCCTATGATGTGGCGTTCATAAAATCTGTTCAGGCACAGTCCGCCACGCTCTCAGATAACACAAGGGCTCTAAGAAATAATGGTGGCCCGGCCGT
AAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCATGATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCCACCAGGTGCAAGGGGAATGTAAAGGCCTTTCACTTTCCCTGAAGGTGATCGGAAGCTCTTTGCATGGTGAGCCTCTCCCGACACGGGAGAGTGCAAAGAGAAAGCTCTT
AAATGGGGAACCAATCTCAGATTATTACAAAGAAGGGCTTCTTAGATGCTTGGAAACTAGTATTGATGCCCTCAATGAGGAAACAAGGGAGTGATT

>TRINITY_DN107813_c0_g1_i10 from sample A3 / Example unspliced isoform:

GTCCGCCACGCTCTCAGATAACACAAGGGCTCTAAGAAATAATGGTGGCCCGGCCGTAAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCGTGATGGAAAGGCAGCCGATTCGCGTTGCAGGGATTTCCACCAGGCAGCCGATTCGCGTTGCAGGGCTTTCGACGTGATAATTCTTAGAGT
CCAGGTTTCAAATCTCCCTGTATTTTTCTTTTTCCTTTTGGCATTTAGTTTTCCCACATGAATTTATATTGGTTATTTGATTTATGCTTGGGAAGCCTCTAATGCCTGGCTAACTCTTATGAGTAGATTATATTTGTTAGATGGTTTTTTCGTCTCAGGGTTTTCAAATGATCGAGGCTTTAGGGTTCCTATGATGTGGC
GTTCATAAAATCTGTTCAGGgttagcgttagtttaattattaatatttccattaataattgtgttacaagctaggagctgtctgttttgcacagtatctgacaaattcttttgcattgattcctacatgctttcccatgcttttaactagCACAGTCCGCCACGCTCTCAGATAACACAAGGGCTCTAAGAAATAATGGT
GGCCCGGCCGTAAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCATGATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCCACCAGGTGCAAGGGGAATGTAAAGGCCTTTCACTTTCCCTGAAGGTGATCGGAAGCTCTTTGCATGGTGAGCCTCTCCCGACACGGGAGAGTGCAAAG
AGAAAGCTCTTAAATGGGGAACCAATCTCAGATTATTACAAAGAAGGGCTTCTTAGATGCTTGGAAACTAGTATTGATGCCCTCAATGAGGAAACAAGGGAGTGATT

Intron 5 GTGATAATTC ... ACTAGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.46	10	5	47
A2	83.67	8	9	41
A3	86.05	6	10	37
A4	86.36	3	11	19
A5	100	0	3	17
A6	68.33	19	5	41
A7	67.02	31	17	63
A8	72.31	18	11	47
A9	55.88	45	9	57
A10	64.38	26	4	47

>TRINITY_DN107813_c0_g1_i6 from sample A3 / Example spliced isoform

GTCCGCCACGCTCTCAGATAACACAAGGGCTCTAAGAAATAATGGTGGCCCGGCCGTAAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCGTGATGGAAAGGCAGCCGATTCGCGTTGCAGGGATTTCCACCAGGCAGCCGATTCGCGTTGCAGGGCTTTCGACTCCGCCACGCTCTCAGA
TAACACAAGGGCTCTAAGAAATAATGGTGGCCCGGCCGTAAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCATGATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCCACCAGGTGCAAGGGGAATGTAAAGGCCTTTCACTTTCCCTGAAGGTGATCGGAAGCTCTTTGCATGGTGA
GCCTCTCCCGACACGGGAGAGTGCAAAGAGAAAGCTCTTAAATGGGGAACCAATCTCAGATTATTACAAAGAAGGGCTTCTTAGATGCTTGGAAACTAGTATTGATGCCCTCAATGAGGAAACAAGGGAGTGATT

>TRINITY_DN107813_c0_g1_i10 from sample A3 / Example unspliced isoform:

GTCCGCCACGCTCTCAGATAACACAAGGGCTCTAAGAAATAATGGTGGCCCGGCCGTAAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCGTGATGGAAAGGCAGCCGATTCGCGTTGCAGGGATTTCCACCAGGCAGCCGATTCGCGTTGCAGGGCTTTCGACgtgataattcttagagt
ccaggtttcaaatctccctgtatttttctttttccttttggcatttagttttcccacatgaatttatattggttatttgatttatgcttgggaagcctctaatgcctggctaactcttatgagtagattatatttgttagatggttttttcgtctcagggttttcaaatgatcgaggctttagggttcctatgatgtggc
gttcataaaatctgttcagggttagcgttagtttaattattaatatttccattaataattgtgttacaagctaggagctgtctgttttgcacagtatctgacaaattcttttgcattgattcctacatgctttcccatgcttttaactagcacagTCCGCCACGCTCTCAGATAACACAAGGGCTCTAAGAAATAATGGT
GGCCCGGCCGTAAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCATGATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCCACCAGGTGCAAGGGGAATGTAAAGGCCTTTCACTTTCCCTGAAGGTGATCGGAAGCTCTTTGCATGGTGAGCCTCTCCCGACACGGGAGAGTGCAAAG
AGAAAGCTCTTAAATGGGGAACCAATCTCAGATTATTACAAAGAAGGGCTTCTTAGATGCTTGGAAACTAGTATTGATGCCCTCAATGAGGAAACAAGGGAGTGATT

Intron 6 GTGATAATTC ... TATTTGTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	92.48	4	5	47
A2	83.43	5	9	25
A3	86.13	7	10	43
A4	91.97	3.5	11	40
A5	100	0	3	15
A6	73.81	9	5	24
A7	82.37	7.5	17	35
A8	77.22	6.5	11	22
A9	96	1	9	24
A10	85	3	4	17

>TRINITY_DN107813_c0_g1_i8 from sample A3 / Example spliced isoform

GTCCGCCACGCTCTCAGATAACACAAGGGCTCTAAGAAATAATGGTGGCCCGGCCGTAAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCGTGATGGAAAGGCAGCCGATTCGCGTTGCAGGGATTTCCACCAGGCAGCCGATTCGCGTTGCAGGGCTTTCGACATGGTTTTTTCGTCTCA
GGGTTTTCAAATGATCGAGGCTTTAGGGTTCCTATGATGTGGCGTTCATAAAATCTGTTCAGGCTTTCCCATGCTTTTAACTAGCACAGTCCGCCACGCTCTCAGATAACACAAGGGCTCTAAGAAATAATGGTGGCCCGGCCGTAAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCATG
ATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCCACCAGGTGCAAGGGGAATGTAAAGGCCTTTCACTTTCCCTGAAGGTGATCGGAAGCTCTTTGCATGGTGAGCCTCTCCCGACACGGGAGAGTGCAAAGAGAAAGCTCTTAAATGGGGAACCAATCTCAGATTATTACAAAGAAGGGCTTCTTAGATGCTTGGAA
ACTAGTATTGATGCCCTCAATGAGGAAACAAGGGAGTGATT

>TRINITY_DN107813_c0_g1_i10 from sample A3 / Example unspliced isoform:

GTCCGCCACGCTCTCAGATAACACAAGGGCTCTAAGAAATAATGGTGGCCCGGCCGTAAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCGTGATGGAAAGGCAGCCGATTCGCGTTGCAGGGATTTCCACCAGGCAGCCGATTCGCGTTGCAGGGCTTTCGACgtgataattcttagagt
ccaggtttcaaatctccctgtatttttctttttccttttggcatttagttttcccacatgaatttatattggttatttgatttatgcttgggaagcctctaatgcctggctaactcttatgagtagattatatttgttagATGGTTTTTTCGTCTCAGGGTTTTCAAATGATCGAGGCTTTAGGGTTCCTATGATGTGGC
GTTCATAAAATCTGTTCAGGGTTAGCGTTAGTTTAATTATTAATATTTCCATTAATAATTGTGTTACAAGCTAGGAGCTGTCTGTTTTGCACAGTATCTGACAAATTCTTTTGCATTGATTCCTACATGCTTTCCCATGCTTTTAACTAGCACAGTCCGCCACGCTCTCAGATAACACAAGGGCTCTAAGAAATAATGGT
GGCCCGGCCGTAAATAAACCTCACAATCCCTTCTGATTTATTCAGCGATAAGTTGCATGGTCCATGATGGAAAGGCAGCCGATTCGCGTTGCAGGGCTTTCCACCAGGTGCAAGGGGAATGTAAAGGCCTTTCACTTTCCCTGAAGGTGATCGGAAGCTCTTTGCATGGTGAGCCTCTCCCGACACGGGAGAGTGCAAAG
AGAAAGCTCTTAAATGGGGAACCAATCTCAGATTATTACAAAGAAGGGCTTCTTAGATGCTTGGAAACTAGTATTGATGCCCTCAATGAGGAAACAAGGGAGTGATT