NA ----> NA

Intron 1 GCTGAAAATG ... GAGGGAAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.42	178	97	28
A2	47.47	125.5	113	15
A3	45.03	122.5	100	21
A4	42.27	20.5	15	2
A5	41.36	55.5	39	18
A6	41.43	17	12	3
A7	33.69	412.5	209	47
A8	36.78	208.5	121	34
A9	38.63	401.5	252	93
A10	34.56	541.5	285	79

>TRINITY_DN41589_c2_g2_i9 from sample A9 / Example spliced isoform

AGAAAATGTTCAAAACCAACCGCAGCAGGGAGATGTCCCTATTCAAACAAGTCTTTTTAACGGCTGCCAGACAACCAAACTGTTATGCCAGAGCCCTCCCACTCATCACCGTAGAAGCCCTAGCCTTGCCATGGAAACCCTAGCCTCGCCATGGAAACCCTAGTCGACCCTTTCATAAAAAAATTGAGCCGACGATATCT
ATAAGTTGGATTCAAAAGATACGTATTCTGATTCGCAAACAAGAACAAGGGGCGAGACATGGCAGAGCACTTCAATACGAAAAGGCCAAAGGAAGAAAATGAAGAAGATCAAGATCAAGATCCAAGGGAAGAAAATGCAGAATATCAAGATCAAACCATGCAACTGAAAATGAAGAAGATCGAAGGGAAGTAAATGCAGA
AGATCAAGATCAAACCACGCAGGTGCAGGCAAAGCATTTAAAGCGCAAGGGTAAAAATGAAGATGATCTGAGGGAAGACAAAGCATTTAAAGCGCAGGCGGTGAAAATGAAGAAGATCGAAGGGAAGTAGAAGTAAATGCAGAAGATCAAGATCAAACCATGCAGCCAAAGCATTTAAAGCGCAGGGGTGAAAATGAAGA
AGATCGAAGGGAAGTAAATGCAGAAGATCAAGATCAAACCACGCAGGCAAAGCATTTAAAGCGCAGCGGTGAAAATGAAGAAGATCGAAAGGAAGTAAATGCAGAAGATCAAGATCAAACTAAGCAGGCAATGCAGGCAAAGCGCAGGGGTGAAAATGAAGGGGAAGTGAATCAAGAAGATCAAGATCAAACCATGCAGG
CAAAGCATTTAAAGCGCAGGGGTGAAAATGAAGAAGATCAAGACATGGCAGATACGAAGAGGCCAACGAAAAAAAATGGAAAAGATCTTATGTCATGGGCACAATCAGACATAGCTGTGAATGGAGTATCCTCATCTTCCAGCTTCCACAATGAGAACCAAGCTCAA

>TRINITY_DN41589_c2_g2_i2 from sample A9 / Example unspliced isoform:

TAAAATTGGATTCAAAATATATCTCTCTTTCGCGAATAAGAAGTGAGACATGGCAGAGCACTTCAATAATGAAGAAGTTCAAGATCAAGATCGAATGGAAGAAAATGCAGAAGATCAAGATCAAACCATATTGCAGGCAAAGCATTTAAAGCGCAGGgctgaaaatgaagaagatcgaagggaagtaaatgcagaagatc
aagatcaaaccatgcaggcaaagcatttaaagcgcaagggtaaaaatgaagatgatctgagggaagagGGTGAAAATGAAGAAGATCGAAGAAATGCAGAAGATCAAGATCAAACCATGCAGGCAAAGCATTTAAAGCGCAGGAGTGAAAATGAAGAAGATCGAAGGGAAGTAAATGCAGAAAATCAACATCAAACCATG
CAGGCAAAGCGTTTAAAGCGCACGGGTGAAAATGAAGAAGATCGAAGGGAAGTAAATGCAGAAGATCAAGATCAAACCACGCAGCGGTGAAAATGAAGAAGATCGAAAGGAAGTAAATGCAGAAGATCAAGATCAAACTAAGCAGGCAATGCAGGCAAAGCGCAGGGGTGAAAATGAAGAGGCAAAGCATTTAAAGCGCA
GGGGTGAAAATGAAGAAGATCAAGACATGGCAGATACGAAGAGGCCAACGAAAAAAAATGGAAAAGATCTTATGTCATGGGCACAATCAGACATAGCTGTGAATGGAGTATCCTCATCTTCCAGCTTCCACAATGAGAACCAAGCTCAA

Intron 2 GCAAATCATT ... GCGCATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	9.84	339	9	37
A2	9.39	222	5	23
A3	4.92	290	0	15
A4	4	24	0	1
A5	5.43	122	0	7
A6	0	28	0	0
A7	8.33	770	18	70
A8	7.24	423	11	33
A9	7.47	793	20	64
A10	7.93	1056	16	91

>TRINITY_DN118485_c0_g1_i3 from sample A1 / Example spliced isoform

TGTTATGCCAGAGCCCTCCCACTCATCACCGTAGAAGCCCTAGCCTTGCCATGGAAACCCTAGCCTCGCCATGGAAACCCTAGTCGACCCTTTCATAAAAAAATTGAGCCGACGATATCTATAAGTTGGATTCAAAAGATACGTATTCTGATTCGCAAACAAGAACAAGGGGCGAGACATGGCAGAGCACTTCAATACGA
AAAGGCCAAAGGAAGAAAATGAAGAAGATCAAGATCAAGATCCAAGGGAAGAAAATGCAGAATATCAAGATCAAACCATGCAACCAAAGCATTTAAAGCGCAGCGGTGAAAATGAAGAAGATCGAAGGGAAGTGAATGCAGAAGATCAAGATCAAACCATGCAGCCAAAGCATTTAAAGCGCAGGGGTGAAAATGAAGAA
GATCGAAGGGAAGTAAATGCGGAAGATCAAGATCAAACCACGCAGGCAAAGCATTTAAAGCGCAGCGGTGAAAATGAAGAAGATCGAAAGGAAGTAAATGCAGAAGATCAAGATCAAACTAAGCAGGCAATGCAGGCAAAGCGCAGGGGTGAAAATGAAGAAGATCGAAGGGAAGTAAATGCAGAAGATCAAGATCAAAC
CATGCAGGCAAAGCATTTAAAGCGCAAGGGTAAAAATGAAGATGATCTGAGGGAAGAACGCAGGCAAAGCATTTAAAGCGCAGCGGTGAAAATGAAGAAGATCGAAGGGAAGTGAATGCAGAAGATCAAGATCAAACCATGCAGGCAAAGAAGGGAAGTAAATGCAGAAAATCAAGATCAAACCATGCAGGCAAAGCATT
TAAAGCGCAGGGGTGAAAATGAAGAAGATCGAAGGGAAGTAAATGCAGAAGATCAAGATCAAAGCATGCACGCAAAGCATTTAAAGCGCAGGGGCGAAAATGAAGAAGATCAAGCCAGGGCAGATACGAAGAGGCCAATGGAAAAAAATGGAAAAGATCTTACGTCACCAGACATAGCTGTGAATGGGGTATCGTCATCA
TCCACCTTCCACAATGAGAACCAAGCTCAA

>TRINITY_DN118485_c0_g1_i4 from sample A1 / Example unspliced isoform:

GAAGATCGAAGGGAAGTAAATGCGGAAGATCAAGATCAAACCACAATGCAGAAGATCAAGATCAAACCATGCAGgcaaatcatttaaagcgcatgcagGCAAAGCATTTAAAGCGCAGGGGTGAAAATGAAGAAGATCGAAGGGAAGTAAATGCGGAAGATCAAGATCAAACCACGCAGGCAAAGCATTTAAAGCGCAGC
GGTGAAAATGAAGAAGATCGAAAGGAAGTAAATGCAGAAGATCAAGATCAAACTAAGCAGGCAATGCAGGCAAAGCGCAGGGGTGAAAATGAAGAAGATCGAAGGGAAGTAAATGCAGAAGATCAAGATCAAACCATGCAGGCAAAGCATTTAAAGCGCAAGGGTAAAAATGAAGATGATCTGAGGGAAGAACGCAGGCA
AAGCATTTAAAGCGCAGCGGTGAAAATGAAGAAGATCGAAGGGAAGTGAATGCAGAAGATCAAGATCAAACCATGCAGGCAAATCATTTAAAGCGCATGGGTGAAAATGAAGAAGATCGAAGGGAAGTGAATGCAGAAGATCAAGATCAAACAAATGCAGAAAATCAAGATCAAACCATGCAGGCAAAGCATTTAAAGCG
CAGGGGTGAAAATGAAGAAGATCGAAGGGAAGTAAATGCAGAAGATCAAGATCAAAGCATGCACGCAAAGCATTTAAAGCGCAGGGGCGAAAATGAAGAAGATCAAGCCAGGGCAGATACGAAGAGGCCAATGGAAAAAAATGGAAAAGATCTTACGTCACCAGACATAGCTGTGAATGGGGTATCGTCATCATCCACCT
TCCACAATGAGAACCAAGCTCAA