NA ----> NA

Intron 1 GTATTGGTCT ... TGACGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	6.52	20	0	1.5
A2	5.26	34	0	2
A3	22.57	17	0	5
A4	0	5	0	0
A5	11.89	21	0	3
A6	9.73	130	1.5	15
A7	7.54	242	0.5	21.5
A8	6.66	245	2.5	18.5
A9	7.08	277	0.5	22.5
A10	8.11	190	0	18

>TRINITY_DN34948_c0_g1_i1 from sample A8 / Example spliced isoform

CGGACGGCTAGGCTATCAAATTGTTGCACGGGAATGTGTCACAGATCACAGCTGGCTCGGTGGCTTTATGAGACAGTTGAAGTTTCCACAAATTCATATGGTTTGTTGAAAGCATGATGACTCAGTCCACGGATGCCAAATTTAATTAAATAATGCTATCGGATCGTTGGACGAGTTTGTCTTCATCTCATCAAAATCTA
GTTGCAGTCATAACATACTTCAGCAATCGGCAGTGAAGACGTAGAAGAAACCCGAGATCATATGAATGCTTGCTTCATATTTAGTTTATGCTCAGTAAATTGAGATTCTTCAATGGATTTCAAGCTTTCATAGAAGAAGTTCACGTGCATTTCTGTTATGAATCCGGATTAGAATGGAAGGAATATTGCACGAGAAGGAA
CAAAAATGAAAAGGCATGTGCGCCAACTCAACGAACAAAAGCGAGGATGTTGGAGAGCGTTTTGAAAATTTATGCAACATGAAGAAAGACGGAATTTCAATCAACCAGCTGTAGCAGAATCAGGAATTTGTTATCTGTCTTCATGGCCTATATAAACAGCACAACTTGTCTGGGGTGTGGGGAGAGGATCTCGACAGGAC
TTGAGAGATTTGTCTTTAGAAGCTCTGTAATTCTCTTTGTTTTATCTGTACGAGAAAATAAGAAGAGGGAGAATACTGCCAGAAGTAGTTTTTGGGCATGCAAAAAGATCTTGTTATTTTTATCCAGAGAGATTTGTAACCAAAGATTACTGTCATGATTGCTCTAAGACTCCTACAATTGTTTCAGTAATACTGCACTT
TGTTGTTGTTTTCTGCTTATCTGGTTGAATGGAATTAAATGCAATTTCTTGGTTCATTCCAGCTTTTGACCTTTGGTGTTTAACCAAATATTACTGCCATGATTGTTCTAAT

>TRINITY_DN34948_c0_g1_i3 from sample A8 / Example unspliced isoform:

AATGCTTGCTTCATATTTAGTTTgtattgttctcttaactttgcttcctaaactcgttatttatattttcaccttcctttccgaaaacttctattttgaaattctgtgcggaaatattgaatgggattatagtttgaagtttttggattttctttacagtgtaattaagtttttgtattgtttttcttttttatgatgta
cagATGCTCAGTAAATTGAGATTCTTCAATGGATTTCAAGCTTTCATAGAAGAAGTTCACGTGCATTTCTGTTATGAATCCGGATTAGAATGGAAGGAATATTGCACGAGAAGGAACAAAAATGAAAAGGCATGTGCGCCAACTCAACGAACAAAAGCGAGGATGTTGGAGAGCGTTTTGAAAATTTATGCAACATGAAG
AAAGACGGAATTTCAATCAACCAGCTGTAGCAGAATCAGGAATTTGTTATCTGTCTTCATGGCCTATATAAACAGCACAACTTGTCTGGGGTGTGGGGAGAGGATCTCGACAGGACTTGAGAGATTTGTCTTTAGAAGCTCTGTAATTCTCTTTGTTTTATCTGTACGAGAAAATAAGAAGAGGGAGAATACTGCCAGAA
GTAGTTTTTGGGCATGCAAAAAGATCTTGTTATTTTTATCCAGAGAGATTTGTAACCAAAGATTACTGTCATGATTGCTCTAAGACTCCTACAATTGTTTCAGTAATACTGCACTTTGTTGTTGTTTTCTGCTTATCTGGTTGAATGGAATTAAATGCAATTTCTTGGTTCATTCCAGCTTTTGACCTTTGGTGTTTAAC
CAAATATTACTGCCATGATTGTTCTAAT

Intron 2 GTAATTAAGT ... TGATGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	9.09	11.7	0	1
A2	14.81	13.7	0	1.3
A3	33	9	2.7	3.3
A4	-1	1.7	0	0
A5	18.06	11.7	0.7	2
A6	16.08	69	3.3	10.7
A7	13.92	119	0	14.3
A8	13.5	119.3	1.7	12.7
A9	13.9	133.7	0.7	15
A10	14.78	96	1.3	12

>TRINITY_DN97085_c0_g1_i4 from sample A3 / Example spliced isoform

GTTATTTATATTTTCACCTTCCTTTCCGAAAACTTCTATTTTGAAATTCTGTGCGGAAATATTGAATGGGATTATAGTTTGAAGTTTTTGGATTTTCTTTACAGTATGCTCAGTAAATTGAGATTCTTCAATGGATTTCAAGCTTTCATAGAAGAAGTTCACGTGCATTTCTGTTATGAATCCGGATTAGAATGGAAGGA
ATATTGCACGAGAAGGAACAAAAATGAAAAGGCATGTGCGCCAACTCAACGAACAAAAGCGAGGATGTTGGAGAGCGTTTTGAAAATTTATGCAACATGAAGAAAGACGGAATTTCAATCAACCAGCTGTAGCAGAATCAGGAATTTGTTATCTGTCTTCATGGCCTATATAAACAGCACAACTTGTCTGGGGTGTGGGG
AGAGGATCTCGACAGGACTTGAGAGATTTGTCTTTAGAAGCTCTGTAATTCTCTTTGTTTTATCTGTACGAGAAAATAAGAAGAGGGAGAATACTGCCAGAAGTAGTTTTTGGGCATGCAAAAAGATCTTGTTATTTTTATCCAGAGAGATTTGTAACCAAAGATTACTGTCATGATTGCTCTAAGACTCCTACAATTGT
TTCAGTAATACTGCACTTTGTTGTTGTTTTCTGCTTATCTGGTTGAATGGAATTAAATGCAATTTCTTGGTGC

>TRINITY_DN97085_c0_g1_i1 from sample A3 / Example unspliced isoform:

GTTATTTATATTTTCACCTTCCTTTCCGAAAACTTCTATTTTGAAATTCTGTGCGGAAATATTGAATGGGATTATAGTTTGAAGTTTTTGGATTTTCTTTACAGTgtaattaagtttttgtattgtttttcttttttatgatgtacagATGCTCAGTAAATTGAGATTCTTCAATGGATTTCAAGCTTTCATAGAAGAAG
TTCACGTGCATTTCTGTTATGAATCCGGATTAG