sp|A0A1S3ZP85|OBL1_TOBAC ----> Triacylglycerol lipase OBL1

Intron 1 GTACTTCACT ... TTTATGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.41	49	31	16
A2	18.92	15	5	2
A3	29.13	45	22	15
A4	17.07	17	6	1
A5	20	12	6	0
A6	25.81	161	75	37
A7	24.58	224	85	61
A8	24.73	213	84	56
A9	29.21	228	119.2	69
A10	22.11	192	71	38

>TRINITY_DN120882_c0_g1_i2 from sample A1 / Example spliced isoform

AGGCTTTCCAAAATAACATAGTTGTTGATAAGATCTAAATTAGTAACATGGTAAAGTATTAGAATGGCAATAACTTGTACATTGTTTCAAATATACGTTGTCTTGAAATGGCAATACCTTGAACTGAAAGAATCATGGCGTGGCAAATATCCAGAGATGTGAGTGCCCAGCAGTGATCTACTTCTTTGCAATTAGTTAAT
CACTCGTATAAACAAATCCTCTGGACAAGGAGGTCGTGTGTTCAACTTTCCTTCCGTCCTCACAATATGACGACCAGCAGTAACTACTTGATAACTCGTCCGGAGAGAATCTGCGTCTCAAAGCTTTTACTGAGTCTCATTTTGCCTCGGGGATTGGGATCTGATGTTTTAGAATGTCCAGAATCCAACATATTGAAGGG
GACTGAGAACTGGGAAATCAAGCTTTCTCTTACATTAATAAAATTGATCAAGTTTGCTGCCGTGCCATTAGCCGAGCTTGGGATCTGTATAGAATTCCTTTTAAACCTGCTTTCTCACAATGGCGGACTGGGAAAAATGTTGTTCAGGCTACTCACTGGCCGAAGCATCGTTATACCGAGGAGAGGGTCCGAGTCGTTTC
TAAGTTTCGTCGGCCTTCTTGAGCATCGCGTCAACTTGTTTGATGGTGGAATTAGTGGGGCTATTGACTCGACCACTGAACCTGGGAATAGGTTCTATGCAGACCTTTGCATCATGGCTTCAACTGTAGCTTATGAAAATAAACTGGTTATCAGTAACAGAGTCACAGAACATTGGAAGATGCATTTCCTTGAGTTCTTC
GAATGCTGGAATGATCACTTACAAAAGAAATCGACGAAAGCGTTTATATTATGTGATAAGAAAGTAGACGCCCAGCTAATAGTGATAGCCTTCAGAGGTACAGAACCATTTGATGCAGATGATTGGGAAACAGATTTCGATTTCTCATGGTATCAACTCAGCCAATTTGGAAAGGTTCATCTTGGATTTCTGGAAGCTTT
AGGTCTTGCAAACCGTTCAGAAAAAGCTGAGATCTTCGACAATCATAGTAGTTCTGTACTCACGGCCAGTTATGTGCATTCTTTTGAAATTGATAAAGAAGATCCTGTAAAGCCTTTGGCTTATTATGCTCTTCGTAAGAAACTGAAAGAATTATTACAAGTTCACAGAAATGCAAAATTCATAGTGACAGGCCACAGCC
TTGGTGGCGCCTTGGCTGTGCTGTTTTCAGCAATATTGTTTATGCACAAGGAAGAGATGCTGTTGGAGAAACTGTTGGCTGTGTATACTTTCGGGCAGCCGAGAGTTGGGGATGAAGTGTTCGCGAAGTTTATGAACAAGAATCTAAACAATCCTGTGCCCAGATACTTTAGACTTGTCTACTGCAATGACATCGTTCCC
AGATTACCCTTCGATGATGATATATTTCTGTACAAGCACTTTGGAGTATGCCTTTACTACAACAGCTGCTACCATGAGAAGAATTTTCCTGAGGAGCCCAACAGAAATTTCTCCTTATTACACTTCTTTCCCGTACACCTAAATGCAGTGTGGGAATTGGTTCAGAGTTTATTTCTGCAATATATAAAGGGAAGGGAATT
TAAAGAAACCAGATTGTCGATATTCTCGAGAATACTGGGGGTTATGGTTCCGGGGGTTTCGGCTCATAGCCCCGTAAATTACATAAACGCTGTAAGATTGGGTCCATCGACGCTGGATCCAACAATGTCCAAAATGGTTCTGACAAAGTATCTGAAGGAGTAGGCATTCATCTCCCTATAAACACTTAATATCAGAATTC
TGTACACACGGCGTGTCCACCGTTGAAGTTTGCTTTAAGCTTCCATTATCTCAATTGATTTCTTTTCTTAGGCTTAGAAATGGATCATGATGGGCCATTACTGT

>TRINITY_DN120882_c0_g1_i3 from sample A1 / Example unspliced isoform:

AGGCTTTCCAAAATAACATAGTTGTTGATAAGATCTAAATTAGTAACATGGTAAAGTATTAGAATGGCAATAACTTGTACATTGTTTCAAATATACGTTGTCTTGAAATGGCAATACCTTGAACTGAAAGAATCATGGCGTGGCAAATATCCAGAGATGTGAGTGCCCAGCAGTGATCTACTTCTTTGCAATTAGTTAAT
CACTCGTATAAACAAATCCTCTGGACAAGGAGGTCGTGTGTTCAACTTTCCTTCCGTCCTCACAATATGACGACCAGCAGTAACTACTTGATAACTCGTCCGGAGAGAATCTGCGTCTCAAAGCTTTTACTGAGTCTCATTTTGCCTCGGGGATTGGGATCTGATGTTTTAGAATGTCCAGAATCCAACATATTGAAGGG
GACTGAGAACTGGGAAATCAAGCTTTCTCTTACATTAATAAAATTGATCAAGTTTGCTGCCGTGCCATTAGCCGAGCTTGGGATCTGTATAGAATTCCTTTTAAACCTGCTTTCTCACAATGGCGGACTGGGAAAAATGTTGTTCAGGCTACTCACTGGCCGAAGCATCGTTATACCGAGGAGAGGGTCCGAGTCGTTTC
TAAGTTTCGTCGGCCTTCTTGAGCATCGCGTCAACTTGTTTGATGGTGGAATTAGTGGGGCTATTGACTCGACCACTGAACCTGGGAATAGGTTCTATGCAGACCTTTGCATCATGGCTTCAACTGTAGCTTATGAAAATAAACTGGTTATCAGTAACAGAGTCACAGAACATTGGAAGATGCATTTCCTTGAGTTCTTC
GAATGCTGGAATGATCACTTACAAAAGAAATCGACGAAAGCGTTTATATTATGTGATAAGAAAGTAGACGCCCAGCTAATAGTGATAGCCTTCAGAGGTACAGAACCATTTGATGCAGATGATTGGGAAACAGATTTCGATTTCTCATGGTATCAACTCAGCCAATTTGGAAAGGTTCATCTTGGATTTCTGGAAGCTTT
AGGTCTTGCAAACCGTTCAGAAAAAGCTGAGATCTTCGACAATCATAGTAGTTCTGTACTCACGGCCAGTTATGTGCATTCTTTTGAAATTGATAAAGAAGATCCTGTAAAGCCTTTGGCTTATTATGCTCTTCGTAAGAAACTGAAAGAATTATTACAAGTTCACAGAAATGCAAAATTCATAGTGACAGGCCACAGCC
TTGGTGGCGCCTTGGCTGTGCTGTTTTCAGCAATATTGTTTATGCACAAGGAAGAGATGCTGTTGGAGAAACTGTTGGCTGTGTATACTTTCGGGCAGCCGAGAGTTGGGGATGAAGTGTTCGCGAAGTTTATGAACAAGAATCTAAACAATCCTGTGCCCAGATACTTTAGACTTGTCTACTGCAATGACATCGTTCCC
AGATTACCCTTCGATGATGATATATTTCTGTACAAGCACTTTGGAGTATGCCTTTACTACAACAGCTGCTACCATGAGAAGgtacttcactgccactcttcacatggtcttctgaaaaagaaaacttggttttcctttctgatacacttagttatgaaagtttatttattttatgacagAATTTTCCTGAGGAGCCCAAC
AGAAATTTCTCCTTATTACACTTCTTTCCCGTACACCTAAATGCAGTGTGGGAATTGGTTCAGAGTTTATTTCTGCAATATATAAAGGGAAGGGAATTTAAAGAAACCAGATTGTCGATATTCTCGAGAATACTGGGGGTTATGGTTCCGGGGGTTTCGGCTCATAGCCCCGTAAATTACATAAACGCTGTAAGATTGGG
TCCATCGACGCTGGATCCAACAATGTCCAAAATGGTTCTGACAAAGTATCTGAAGGAGTAGGCATTCATCTCCCTATAAACACTTAATATCAGAATTCTGTACACACGGCGTGTCCACCGTTGAAGTTTGCTTTAAGCTTCCATTATCTCAATTGATTTCTTTTCTTAGGCTTAGAAATGGATCATGATGGGCCATTACT
GT

Intron 2 GTGATTGTAT ... ATTTGTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.02	39	16.2	8
A2	8.88	40.4	14.6	3.9
A3	25.64	58	28.6	20
A4	25	33	21.8	11
A5	12.2	36	8.5	5
A6	15.69	86	34	16
A7	20.37	129	37.9	33
A8	24	57	27.6	18
A9	19.27	104.8	43.1	25
A10	18.23	143.6	55.5	32

>TRINITY_DN107611_c0_g1_i1 from sample A3 / Example spliced isoform

ATTCCCATGAAAATGGAATTTTCAAGAACAGGATATAGTTATAAATTTGTAATGGGGCTTTCCAAAATAGAATTTTCAGACATGAGGCTTTCCAAAATAACATAGTTGTTGATAAGATCTAAATTAGTAACATGGTAAAGTATTAGAATGGCAATACCTTGTACATTGTTTCAAATATACGTTGTCTTGAAATGGCAATA
CCTTGAACTGAAAGAATCATGGCGTGGCAAATATCCAGAGATGTGAGTGCCCAGCAGTGATCTACTTCTTTGCAATTAGTTAATCACTCGTATAAACAAATCCTCTGGACAAGGAGGTCGTGTGTTCAACTTTCCTTCCGTCCTCACAATATGACGACCAGCAGTAACTACTTGATAACTCGTCCGGAGAGAATCTGCGT
CTCAAAGCTTTTACTGAGTCTCATTTTGCCTCGGGGATTGGGATCTGATGTTTTAGAATGTCCAGAATCCAACATATTGAAGGGGACTGAGAACTGGGAAATCAAGCTTTCTCTTACATTAATAAAATTGATCAAGTTTGCTGCCGTGCCATTAGCCGAGCTTGGGATCTGTATAGAATTCCTTTTAAACCTGCTTTCTC
ACAATGGCGGACTGGGAAAAATGTTGTTCAGGCTACTCACTGGCCGAAGCATCGTTATACCGAGGAGAGGGTCCGAGTCGTTTCTAAGTTTCGTCGGCCTTCTTGAGCATCGCGTCAACTTGTTTGATGGTGGAATTAGTGGGGCTATTGACTCGACCACTGAACCTGGGAATAGGTTCTATGCAGACCTTTGCATCATG
GCTTCAACTGTAGCTTATGAAAATAAACTGGTTATCAGTAACAGAGTCACAGAACATTGGAAGATGCATTTCCTTGAGTTCTTCGAATGCTGGAATGATCACTTACAAAAGAAATCGACGAAAGCGTTTATATTATGTGATAAGAAAGTAGACGCCCAGCTAATAGTGATAGCCTTCAGAGGTACAGAACCATTTGATGC
AGATGATTGGGAAACAGATTTCGATTTCTCATGGTATCAACTCAGCCAATTTGGAAAGGTTCATCTTGGATTTCTGGAAGCTTTAGGTCTTGCAAACCGTTCAGAAAAAGCTGAGATCTTCGACAATCATAGTAGTTCTGTACTCACGGCCAGTTATGTGCATTCTTTTGAAATTGATAAAGAAGATCCTGTAAAGCCTT
TGGCTTATTATGCTCTTCGTAAGAAACTGAAAGAATTATTACAAGTTCACAGAAATGCAAAATTCATAGTGACAGGCCACAGCCTTGGTGGCGCCTTGGCTGTGCTGTTTTCAGCAATATTGTTTATGCACAAGGAAGAGATGCTGTTGGAGAAACTGTTGGCTGTGTATACTTTCGGGCAGCCGAGAGTTGGGGATGAA
GTGTTCGCGAAGTTTATGAACAAGAATCTAAACAATCCTGTGCCCAGATACTTTAGACTTGTCTACTGCAATGACATCGTTCCCAGATTACCCTTCGATGATGATATATTTCTGTACAAGCACTTTGGAGTATGCCTTTACTACAACAGCTGCTACCATGAGAAGAATTTTCCTGAGGAGCCCAACAGAAATTTCTCCTT
ATTACACTTCTTTCCCGTACACCTAAATGCAGTGTGGGAATTGGTTCAGAGTTTATTTCTGCAATATATAAAGGGAAGGGAATTTAAAGAAACCAGATTGTCGATATTCTCGAGAATACTGGGGGTTATGGTTCCGGGGGTTTCGGCTCATAGCCCCGTAAATTACATAAACGCTGTAAGATTGGGTCCATCGACGCTGG
ATCCAACAATGTCCAAAATGGTTCTGACAAAGTATCTGAAGGAGTAGGCATTCATCTCCCTATAAACACTTAATATCAGAATTCTGTACACACGGCGTGTCCACCGTTGAAGTTTGCTTTAAGCTTCCATTATCTCAATTGATTTCTTTTCTTAGGCTTAGAAATGGATCATGATGGGCCATTACTGTTTTACCTTACAT
AATGAACCAATATGCTTATTACATGCTTAAA

>TRINITY_DN107611_c0_g1_i4 from sample A3 / Example unspliced isoform:

ATTCCCATGAAAATGGAATTTTCAAGAACAGGATATAGTTATAAATTTGTAATGGGGCTTTCCAAAATAGAATTTTCAGACATGAGGCTTTCCAAAATAACATAGTTGTTGATAAGATCTAAATTAGTAACATGGTAAAGTATTAGAATGGCAATACCTTGTACATTGTTTCAAATATACGTTGTCTTGAAATGGCAATA
CCTTGAACTGAAAGAATCATGGCGTGGCAAATATCCAGAGATGTGAGTGCCCAGCAGTGATCTACTTCTTTGCAATTAGTTAATCACTCGTATAAACAAATCAACAAATCCTCTGGACAAGGAGGTCGTGTGTTCAACTTTCCTTCCGTCTTTTTATGATATAATTCTTCTACGAAGTTACACCGTGCTCTGTGAATTCT
GTATCTCCTCACAATATGACGACCAGCAGTAACTACTTGATAACTCGTCCGGAGAGAATCTGCGTCTCAAAGCTTTTACTGAGTCTCATTTTGCCTCGGGGATTGGGATCTGATGTTTTAGAATGTCCAGAATCCAACATATTGAAGGGGACTGAGAACTGGGAAATCAAGCTTTCTCTTACATTAATAAAATTGATCAA
GTTTGCTGCCGTGCCATTAGCCGAGCTTGGGATCTGTATAGAATTCCTTTTAAACCTGCTTTCTCACAATGGCGGACTGGGAAAAATGTTGTTCAGGCTACTCACTGgtgattgtattgatctgtttctttgattgtattgatctgtttcttaatgtatttaaaattaaataggtatttcatgcttcatgctccatgtgt
tagggtttagttgaatgctttgatttgttcagGCCGAAGCATCGTTATACCGAGGAGAGGGTCCGAGTCGTTTCTAAGTTTCGTCGGCCTTCTTGAGCATCGCGTCAACTTGTTTGATGGTGGAATTAGTGGGGCTATTGACTCGACCACTGAACCTGGGAATAGGTTCTATGCAGACCTTTGCATCATGGCTTCAACTG
TAGCTTATGAAAATAAACTGGTTATCAGTAACAGAGTCACAGAACATTGGAAGATGCATTTCCTTGAGTTCTTCGAATGCTGGAATGATCACTTACAAAAGAAATCGACGAAAGCGTTTATATTATGTGATAAGAAAGTAGACGCCCAGCTAATAGTGATAGCCTTCAGAGGTACAGAACCATTTGATGCAGATGATTGG
GAAACAGATTTCGATTTCTCATGGTATCAACTCAGCCAATTTGGAAAGGTTCATCTTGGATTTCTGGAAGCTTTAGGTCTTGCAAACCGTTCAGAAAAAGCTGAGATCTTCGACAATCATAGTAGTTCTGTACTCACGGCCAGTTATGTGCATTCTTTTGAAATTGATAAAGAAGATCCTGTAAAGCCTTTGGCTTATTA
TGCTCTTCGTAAGAAACTGAAAGAATTATTACAAGTTCACAGAAATGCAAAATTCATAGTGACAGGCCACAGCCTTGGTGGCGCCTTGGCTGTGCTGTTTTCAGCAATATTGTTTATGCACAAGGAAGAGATGCTGTTGGAGAAACTGTTGGCTGTGTATACTTTCGGGCAGCCGAGAGTTGGGGATGAAGTGTTCGCGA
AGTTTATGAACAAGAATCTAAACAATCCTGTGCCCAGATACTTTAGACTTGTCTACTGCAATGACATCGTTCCCAGATTACCCTTCGATGATGATATATTTCTGTACAAGCACTTTGGAGTATGCCTTTACTACAACAGCTGCTACCATGAGAAGAATTTTCCTGAGGAGCCCAACAGAAATTTCTCCTTATTACACTTC
TTTCCCGTACACCTAAATGCAGTGTGGGAATTGGTTCAGAGTTTATTTCTGCAATATATAAAGGGAAGGGAATTTAAAGAAACCAGATTGTCGATATTCTCGAGAATACTGGGGGTTATGGTTCCGGGGGTTTCGGCTCATAGCCCCGTAAATTACATAAACGCTGTAAGATTGGGTCCATCGACGCTGGATCCAACAAT
GTCCAAAATGGTTCTGACAAAGTATCTGAAGGAGTAGGCATTCATCTCCCTATAAACACTTAATATCAGAATTCTGTACACACGGCGTGTCCACCGTTGAAGTTTGCTTTAAGCTTCCATTATCTCAATTGATTTCTTTTCTTAGGCTTAGAAATGGATCATGATGGGCCATTACTGTTTTACCTTACATAATGAACCAA
TATGCTTATTACATGCTTAAA

Intron 3 GTATGCAACT ... ATACAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.91	38	17	12
A2	41.03	23	16	7.3
A3	20	32	8	5
A4	30.77	36	16	9.4
A5	23.81	16	5	2.9
A6	21.6	98	27	19.3
A7	18.18	99	22	12.4
A8	25	84	28	12.4
A9	17.24	120	25	22.1
A10	20.21	75	19	10.7

>TRINITY_DN100467_c0_g2_i4 from sample A4 / Example spliced isoform

GTTGTCTTGAAATGGCAATACCTTGAACTGAAAGAATCATGGCGTGGCAAATATCCAGAGATGTGAGTGCCCAGCAGTGATCTACTTCTTTGCAATTAGTTAATCACTCGTATAAACAAATCCTCTGGACAAGGAGGTCGTGTGTTCAACTTTCCTTCCGTCCTCACAATATGACGACCAGCAGTAACTACTTGATAACT
CGTCCGGAGAGAATCTGCGTCTCAAAGCTTTTACTGAGTCTCATTTTGCCTCGGGGATTGGGATCTGATGTTTTAGAATGTCCAGAATCCAACATATTGAAGGGGACTGAGAACTGGGAAATCAAGCTTTCTCTTACATTAATAAAATTGATCAAGTTTGCTGCCGTGCCATTAGCCGAGCTTGGGATCTGTATAGAATT
CCTTTTAAACCTGCTTTCTCACAATGGCGGACTGGGAAAAATGTTGTTCAGGCTACTCACTGGCCGAAGCATCGTTATACCGAGGAGAGGGTCCGAGTCGTTTCTAAGTTTCGTCGGCCTTCTTGAGCATCGCGTCAACTTGTTTGATGGTGGAATTAGTGGGGCTATTGACTCGACCACTGAACCTGGGAATAGGTTCT
ATGCAGACCTTTGCATCATGGCTTCAACTGTAGCTTATGAAAATAAACTGGTTATCAGTAACAGAGTCACAGAACATTGGAAGATGCATTTCCTTGAGTTCTTCGAATGCTGGAATGATCACTTACAAAAGAAATCGACGAAAGCGTTTATATTATGTGATAAGAAAGTAGACGCCCAGCTAATAGTGATAGCCTTCAGA
GGTACAGAACCATTTGATGCAGATGATTGGGAAACAGATTTCGATTTCTCATGGTATCAACTCAGCCAATTTGGAAAGGTTCATCTTGGATTTCTGGAAGCTTTAGGTCTTGCAAACCGTTCAGAAAAAGCTGAGATCTTCGACAATCATAGTAGTTCTGTACTCACGGCCAGTTATGTGCATTCTTTTGAAATTGATAA
AGAAGATCCTGTAAAGCCTTTGGCTTATTATGCTCTTCGTAAGAAACTGAAAGAATTATTACAAGTTCACAGAAATGCAAAATTCATAGTGACAGGCCACAGCCTTGGTGGCGCCTTGGCTGTGCTGTTTTCAGCAATATTGTTTATGCACAAGGAAGAGATGCTGTTGGAGAAACTGTTGGCTGTGTATACTTTCGGGC
AGCCGAGAGTTGGGGATGAAGTGTTCGCGAAGTTTATGAACAAGAATCTAAACAATCCTGTGCCCAGATACTTTAGACTTGTCTACTGCAATGACATCGTTCCCAGATTACCCTTCGATGATGATATATTTCTGTACAAGCACTTTGGAGTATGCCTTTACTACAACAGCTGCTACCATGAGAAGAATTTTCCTGAGGAG
CCCAACAGAAATTTCTCCTTATTACACTTCTTTCCCGTACACCTAAATGCAGTGTGGGAATTGGTTCAGAGTTTATTTCTGCAATATATAAAGGGAAGGGAATTTAAAGAAACCAGATTGTCGATATTCTCGAGAATACTGGGGGTTATGGTTCCGGGGGTTTCGGCTCATAGCCCCGTAAATTACATAAACGCTGTAAG
ATTGGGTCCATCGACGCTGGATCCAACAATGTCCAAAATGGTTCTGACAAAGTATCTGAAGGAGTAGGCATTCATCTCCCTATAAACACTTAATATCAGAATTCTGTACACACGGCGTGTCCACCGTTGAAGTTTGCTTTAAGCTTCCATTATCTCAATTGATTTCTTTTCTTAGGCTTAGAAATGGATCATGATGGGCC
ATTACTGTTTTACCTTACATAATGAACCAATATGCTTATTACATGCTTATTAAAAA

>TRINITY_DN100467_c0_g2_i2 from sample A4 / Example unspliced isoform:

GTTGTCTTGAAATGGCAATACCTTGAACTGAAAGAATCATGGCGTGGCAAATATCCAGAGATGTGAGTGCCCAGCAGTGATCTACTTCTTTGCAATTAGTTAATCACTCGTATAAACAAATCCTCTGGACAAGGAGGTCGTGTGTTCAACTTTCCTTCCGTCCTCACAATATGACGACCAGCAGTAACTACTTGATAACT
CGTCCGGAGAGAATCTGCGTCTCAAAGCTTTTACTGAGTCTCATTTTGCCTCGGGGATTGGGATCTGATGTTTTAGAATGTCCAGAATCCAACATATTGAAGGGGACTGAGAACTGGGAAATCAAGCTTTCTCTTACATTAATAAAATTGATCAAGTTTGCTGCCGTGCCATTAGCCGAGCTTGGGATCTGTATAGAATT
CCTTTTAAACCTGCTTTCTCACAATGGCGGACTGGGAAAAATGTTGTTCAGGCTACTCACTGGTGATTGTATTGATCTGTTTCTTTGATTGTATTGATCTTTTTTTTAATGTATTTAAAATTAAATAGGGATTTCATGCTTCATGCTCCATGTGTTAGGGTTTAGTTGAATGCTTTGATTTGTTCAGGCCGAAGCATCGT
TATACCGAGGAGAGGGTCCGAGTCGTTTCTAAGTTTCGTCGGCCTTCTTGAGCATCGCGTCAACTTGTTTGATGGTGGAATTAGTGGGGCTATTGACTCGACCACTGAACCTGGGAATAGGTTCTATGCAGACCTTTGCATCATGGCTTCAACTGTAGCTTATGAAAATAAACTGGTTATCAGTAACAGAGTCACAGAAC
ATTGGAAGATGCATTTCCTTGAGTTCTTCGAATGCTGGAATGgtatgcaactctttggcttcagtaaagtattattcaatcattaaatatattgatttctctgtgaataattttgcgttctatcaattgatgttgagattgacttgtatacaatcagATCACTTACAAAAGAAATCGAC

Intron 4 GTACTGTTTA ... ATTCCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.67	40	1	8
A2	3.57	27	0	1
A3	20	36	0	9
A4	22.22	35	0	10
A5	14.29	24	0	4
A6	17.29	110	2	23
A7	20.3	125.6	3	32
A8	15.38	99	0	18
A9	16.47	142	0	28
A10	8.26	100	0	9

>TRINITY_DN44756_c0_g1_i1 from sample A7 / Example spliced isoform

TGATATAGTTGTAAATTTGTAATGAGGCTTTCCAAAATAACATAGTTGTTGATAAGATCTAAATTAGTAACATGGTAAAGTATTAGAATGGCAATACCTTGTACATTGTTTCAAATATACGTTGTCTTGAAATGGCAATACCTTGAACTGAAAGAATCATGGCGTGGCAAATATCCAGAGATGTGAGTGCCCAGCAGTGA
TCTACTTCTTTGCAATTAGTTAATCACTCGTATAAACAAATCCTCTGGACAAGGAGGTCGTGTGTTCAACTTTCCTTCCGTCCTCACAATATGACGACCAGCAGTAACTACTTGATAACTCGTCCGGAGAGAATCTGCGTCTCAAAGCTTTTACTGAGTCTCATTTTGCCTCGGGGATTGGGATCTGATGTTTTAGAATG
TCCAGAATCCAACATATTGAAGGTGACTGAGAACTGGGAAATCAAGCTTTCTCTTATATTAATAAAATTGATCAAGTTTGCTGCCGTGCCATTAGCCGAGCTTGGGATCTGTATAGAATTCCTTTTAAACCTGCTTTCTCACAATGGCGGACTGGGAAAAATGTTGTTCAGGCTACTCACTGGCCGAAGCATCGTTATAC
CGAGGAGAGGGTCCGAGTCGTTTCTAAGTTTCGTCGGCCTTCTTGAGCATCGCGTCAACTTGTTTGATGGTGGAATTAGTGGGGCTATTGACTCGACCACTGAACCTGGGAATAGGTTCTATGCAGACCTTTGCATCATGGCTTCAACTGTAGCTTATGAAAATAAACTGGTTATCAGTAACAGAGTCACAGAACATTGG
AAGATGCATTTCCTTGAGTTCTTCGAATGCTGGAATGATCACTTACAAAAGAAATCGACGAAAGCGTTTATATTATGTGATAAGAAAGTAGACGCCCAGCTAATAGTGATAGCCTTCAGAGGTACAGAACCATTTGATGCAGATGATTGGGAAACAGATTTCGATTTCTCATGGTATCAACTCAGCCAATTTGGAAAGGT
TCATCTTGGATTTCTGGAAGCTTTAGGTCTTGCAAACCGTTCAGAAAAAGCTGAGATCTTCGACAATCATAGTAGTTCTGTACTCACGGCCAGTTATGTGCATTCTTTTGAAATTGATAAAGAAGATCCTGTAAAGCCTTTGGCTTATTATGCTCTTCGTAAGAAACTGAAAGAATTATTACAAGTTCACAGAAATGCAA
AATTCATAGTGACAGGCCACAGCCTTGGTGGCGCCTTGGCTGTGCTGTTTTCAGCAATATTGTTTATGCACAAGGAAGAGATGCTGTTGGAGAAACTGTTGGCTGTGTATACTTTCGGGCAGCCGAGAGTTGGGGATGAAGTGTTCGCGAAGTTTATGAACAAGAATCTAAACAATCCTGTGCCCAGATACTTTAGACTT
GTCTACTGCAATGACATCGTTCCCAGATTACCCTTCGATGATGATATATTTCTGTACAAGCACTTTGGAGTATGCCTTTACTACAACAGCTGCTACCATGAGAAGAATTTTCCTGAGGAGCCCAACAGAAATTTCTCCTTATTACACTTCTTTCCCGTACACCTAAATGCAGTGTGGGAATTGGTTCAGAGTTTATTTCT
GCAATATATAAAGGGAAGGGAATTTAAAGAAACCAGATTGTCGATATTCTCGAGAATACTGGGGGTTATGGTTCCGGGGGTTTCGGCTCATAGCCCCGTAAATTACATAAACGCTGTAAGATTGGGTCCATCGACGCTGGATCCAACAATGTCCAAAATGGTTCTGACAAAGTATCTGAAGGAGTAGGCATTCATCTCCC
TATAAACACTTAATATCAGAATTCTGTACACACGGCGTGTCCACCGTTGAAGTTTGCTTTAAGCTTCCATTATCTCAATTGATTTCTTTTCTTAGGCTTAGAAATGGATCATGATGGGCCATTACTGTTTTACCTTACATAATGAACCAATATGCTTATTACATGCTTATTACTTTCAAGTATCATGTTTGTAGCTTATT
ATTTTGCAACTTCCTTCCTTTTTTA

>TRINITY_DN44756_c0_g1_i7 from sample A7 / Example unspliced isoform:

ACAGAGTCACAGAACATTGGAAGgtactgtttaatttctatccagtttgattcttgattttttattcttgattcttgattaaatgattcttgattcttgatttaatttccatccattttttattcttgatttaatgcacattttgatctcatattcctgcagATGCATTTCCTTGAGTTCTTCGAATGCTGGAATGATCA
CTTACAAAAGAAATCGACGAAAGCGTTTATATTATGTGATAAGAAAGTAGACGCCCAGCTAATAGTGATAGCCTTCAGAGGTACAGAACCATTTGATGCAGATGATTGGGAAACAGATTTCGATTTCTCATGGTATCAACTCAGCCAATTTGGAAAGGTTCATCTTGGATTTCTGGAAGCTTTAGGTCTTGCAAACCGTT
CAGAAAAAGCTGAGATCTTCGACAATCATAGTAGTTCTGTACTCACGGCCAGTTATGTGCATTCTTTTGAAATTGATAAAGAAGATCCTGTAAAGCCTTTGGCTTATTATGCTCTTCGTAAGAAACTGAAAGAATTATTACAAGTTCACAGAAATGCAAAATTCATAGTGACAGGCCACAGCCTTGGTGGCGCCTTGGCT
GTGCTGTTTTCAGCAATATTGTTTATGCACAAGGAAGAGATGCTGTTGGAGAAACTGTTGGCTGTGTATACTTTCGGGCAGCCGAGAGTTGGGGATGAAGTGTTCGCGAAGTTTATGAACAAGAATCTAAACAATCCTGTGCCCAGATACTTTAGACTTGTCTACTGCAATGACATCGTTCCCAGATTACCCTTCGATGA
TGATATATTTCTGTACAAGCACTTTGGAGTATGCCTTTACTACAACAGCTGCTACCATGAGAAGAATTTTCCTGAGGAGCCCAACAGAAATTTCTCCTTATTACACTTCTTTCCCGTACACCTAAATGCAGTGTGGGAATTGGTTCAGAGTTTATTTCTGCAATATATAAAGGGAAGGGAATTTAAAGAAACCAGATTGT
CGATATTCTCGAGAATACTGGGGGTTATGGTTCCGGGGGTTTCGGCTCATAGCCCCGTAAATTACATAAACGCTGTAAGATTGGGTCCATCGACGCTGGATCCAACAATGTCCAAAATGGTTCTGACAAAGTATCTGAAGGAGTAGGCATTCATCTCCCTATAAACACTTAATATCAGAATTCTGTACACACGGCGTGTC
CACCGTTGAAGTTTGCTTTAAGCTTCCATTATCTCAATTGATTTCTTTTCTTAGGCTTAGAAATGGATCATGATGGGCCATTACTGTTTTACCTTACATAATGAACCAATATGCTTATTACATGCTTATTACTTTCAAGTATCATGTTTGTAGCTTATTATTTTGCAACTTCCTTCCTTTTTTA