sp|O80763|NRX1_ARATH ----> Probable nucleoredoxin 1

Intron 1 GTAAGAATCT ... TGTTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	57.77	326	446	109
A2	56.75	330	433	59
A3	53.5	339	390	80
A4	46.73	261	229	56
A5	57.89	160	220	32
A6	65.15	1322	2471	319
A7	60.12	1654	2493	266
A8	66.91	1627	3290	411
A9	64.57	1489	2714	246
A10	61.2	1251	1973	174

>TRINITY_DN45572_c0_g5_i2 from sample A6 / Example spliced isoform

GCACCTCCAGAATCAGGAAGGGTGTTTTCTTACCATAAGAATGAAAAACATATATAGTGCAAAGAAACAATGGATGTGAAGGCTGAGTTCCTGAATGGAAAGGGTGATAATTTTGGAACTACTGAGTTCAGTTGGATTGATGATATCCCTATCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGT
ATATCTCCAAACTATTGCACCTACTGCATCAAAATATGGTTTTTATAGGATTCAATGCTTCTTCTGCAATTTGTAGAAGATTAATTTGTCAGGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATAGCTTGACCCTCTCAAAGGTGCCCAAATTCCTGATTATTTGTTTGTAAATGGCAGTTTAGTTTTGT
TAAACTGAATTGGAAATGGTAATTTCATTGTACATTGCCCTG

>TRINITY_DN45572_c0_g5_i1 from sample A6 / Example unspliced isoform:

GCACCTCCAGAATCAGGAAGGGTGTTTTCTTACCATAAGAATGAAAAACATATATAGTGCAAAGAAACAATGGATGTGAAGGCTGAGTTCCTGAATGGAAAGGGTGATAATTTTGGAACTACTGAGTTCAGTTGGATTGATGATATCCCTATCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGT
ATATCTCCAAACTATTGCACCTACTGCATCAAAATATGgtaagaatctatgcaaaaatgtaaaacgagacctgctcttatttcttgttatttgtgttatatgataggaagaatagttatttgtacagtttttgtaatatatatcaaaaaaattgttgtactattttagattatgctgttaatgggctagctttcactgat
ttcactttgctcgtgcgagcatacttatagtgaatgcatacaaaattttagaatagagagtattataagaatcgtggtaaacagagcatttgaaagacttgaagaattttaattactaataacagtgaatcattctgggtcaatctgtcatactctcatgcactaaattatgatgtttctctgcatcattctcaaatgtt
gtttctcattgtgtttggattatagtgtggcccccacgaaaggagtattttgtatcgttgttcttttaaggcatcttgatttcaatctgtcaatgatcatgctatgccctttccataaaaaaatagctggatccacagaaagtccagaaagaatgcatgtatttgtttgtttttgtggatggttactttgaagttaaatc
taacttctgcttttaagttatttggatacgtcctaagttggaagttttcaatctcatgtatatgcaagatagtatctcccttggtagcctcagtacctgcaggagtggttttgatgagggagaaattaggcttcaagttcacaactggggtgcaacctttgcgatttgcaaactgggatagtgaagataaaataaccttc
tgtatgagtggaaggcaagtaaataactagttatgccataggcgttctttcagtgagaaactctgcctgggcaccgaacagattcctcatcactctgcccaaccgccttccgccatgcgtgtttttcaattaactcttctatcgcatgccagctgtgttgcgtgtcgcagtttgaagatggtaatttcactgtacatttt
attttgaacgattattgcactgtaaatggaaggtgtgttgtggttatccaacttccattgcttatgtgggtactaggtttgattctcactgcctgctatatagcagttccagtacctgtgccaaatcataaatttaacgttcctgataacaccagggaccttgaagctaccttacagggattacagggctttcagtgaat
atgattttgaatctcaaatatctagggtttcgttgttttacagGTTTTTATAGGATTCAATGCTTCTTCTGCAATTTGTAGAAGATTAATTTGTCAGGTAATGTTCGTCTCTGTGGAATTTGATTTGGAGTTTTTTTCCTTGTTTTATTGATAATTAGGGTGAAAAAGGTTCTACGGGTGCGCAATGTGTTTAATTCTGA
TGGTTAGGGTTTGGAGTGTTCACATGCTTGCTTGTATCTAGATTTTCACAGCTGGTATGCTAGAAGGGATTATTGTCACATACTATTCTAAACTTAATTATTGCAATGTTGAACGGGGCAGGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATCAGCAAAAATGGACAGCAGGTATGATTGTGATACTTA
TATATACACTCTTTGGTGTCATTCTCTTTGCTTGAAAATTGCATCTATGGA

Intron 2 GTGCCCAAAT ... GGTTTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.41	60.6	102.8	71
A2	50.47	61.5	67.4	57
A3	49.4	70.5	76.9	58
A4	42.81	66.9	71.8	28
A5	68.41	18.9	41.3	39
A6	78.06	85.3	473.3	115
A7	61.34	143.5	323.6	114
A8	64.67	181	505.6	142
A9	65.54	135.8	352.5	150
A10	55.1	104.8	183.8	66

>TRINITY_DN123593_c1_g2_i1 from sample A2 / Example spliced isoform

GGAGTTTGAAGATCCATCAGTATATCTCCAAACTATTGCACCTACTGCATCAAAATATGGTTTTTATAGGATTCAATGCTTCTTCTGCAATTTGTAGAAGATTAATTTGTCAGGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATAGCTTGACCCTCTCAAAGTGGAAATGTGGAGAATGGAAGTGAAGT
TTGTAGTTGTGAATTGTACCAAAAAAACTACTGATTTCCTTAATCAGCAAAAATGGACAGCAGGTCCTAGTATCTGAGCTTGTGGAAAAGACAGATTCTCTGTATTTCTCTGCCCATTGGTGTCCCCC

>TRINITY_DN123593_c1_g2_i4 from sample A2 / Example unspliced isoform:

GTTTTTATAGGATTCAATGCTTCTTCTGCAATTTGTAGAAGATTAATTTGTCAGGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATAGCTTGACCCTCTCAAAGgtgcccaaattcctgattatttgtttgtaaatggcagtttagttttgttaaactgaattggaaatggtaatttcattgtacattgc
cctgtacattttgtatctaactctaaattagcttgtggtgtttgatacacaacccacttcttttaattataaaatgctctttccatatttggagcataatgtacttttcttttgaagttgataatataaataactgtggagttatagacattaaatgtaatgtgtaagaaactgatactacaaaggtaactacttgagcg
ttgttgggtttggcagTGGAAATGTGGAGAATGGAAGTGAAGTTTGTAGTTGTGAATTGTACCAAAAAAACTACTGATTTCCTTAATCAGCAAAAATGGACAGCGGGTATGATTATGATACTTATATATACACTCTTTGGTGTCATTCTCTTTGCTTGAAAATTGTATCTATGGATTGAATTCAATGACCTAGAG

Intron 3 GTAATGTTCG ... AACGGGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	54.87	100.5	104.1	123.5
A2	52.33	89.2	66	113.4
A3	48.41	106.5	81	105.7
A4	41.9	100.3	49	76.8
A5	52.27	42	25	65.4
A6	43.06	307.1	190.2	259.6
A7	42.3	303.1	179.5	247.7
A8	46.21	367.2	262	351.7
A9	41.51	300.5	168.1	246.1
A10	41.98	245.8	135	204.6

>TRINITY_DN124108_c2_g1_i3 from sample A3 / Example spliced isoform

GCACCTCCAGAATCAGGAAGGGTGTTTTCTTACCATAAGAATGAAAAACATATATAGTGCAAAGAAACAATGGATGTGAAGGCTGAGTTCCTGAATGGAAAGGGTGATAATTTTGGAACTACTGAGTTCAGTTGGATTGATGATATCCCTATCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGT
ATATCTCCAAACTATTGCACCTACTGCATCAAAATATGGTTTTTATAGGATTCAATGCTTCTTCTGCAATTCGTAGAAGATTAATTTGTCAGGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATAGCTTGACCCTCTCAAAGTGGAAATGTGGAGAATGGAAGTGAAGTTTGTAGTTGTGAATTGTACCA
AAAAAACTACTGATTTCCTTAATCAGCAAAAATGGACAGCAGGTCCTAGTATCTGAGCTTGTGGAAAAGACAGATTCTCTGTATTTCTCTGCCCATTGGTG

>TRINITY_DN124108_c2_g1_i8 from sample A3 / Example unspliced isoform:

ATGCTATGCCCTTTCCATAAAAAAATAGCTGGATCCACAGAAAGTCCAGAAAGAATGCATGTATTTGTTTGTTTTTGTGGATGGTTACTTTGAAGTTAAATCTAACTTCTGCTTTTAAGTTATTTGGATACGTCCTAAGTTGGAAGTTTTCAATCTCATGTATATGCAAGATAGTATCTCCCTTGGTAGCCTCAGTACCT
GCAGGAGTGGTTTTGATGAGGGAGAAATTAGGCTTCAAGTTCACAACTGGGGTGCAACCTTTGAGATTTGCAAACTGGGATAGTGAAGATAAAATAACCTTCTGTATGAGTGGAAGGCAAGTAAATAACTAGTTATGCCATAGGCGTTCTTTCAGTGAGAAACTCTGCCTGGGCACCGAACAGATTCCTCATCACTCTGC
CCAACCGCCTTCCGCCATGCGTGTTTTTCAATTAACTCTTCTATCGCATGCCAGCTGTGTTGCATGTCGCAGTTTGAAGATGGTAATTTCACTGTACATTTTATTTTGAACGATTATTGCACTGTAAATGGAAGGTGTGTTGTGGTTATCCAACTTCCATTGCTTATGTGGGTACTAGGTTTGATTCTCACTGCCTGCTA
TATAGCAGTTCCAGTACCTGTGCCAAATCATAAATTTAACGTTCCTGATAACACCAGGGACGTTGAAGCTACCTTACAGGGATTACAGGGCTTTCAGTGAATATGATTTTGAATCTCAAATATCTAGGGTTTCGTTGTTTTACAGGTTTTTATAGGATTCAATGCTTCTTCTGCAATTCGTAGAAGATTAATTTGTCAGg
taatgttcgtctctgtggaatttgatttggagtttttttccttgttttattgataattagggtgaaaaaggttctacgggtgcgcaatgtgtttaattctgatggttagggtttggagtgttcacatgcttgcttgtatctagattttcacagctggtatgctagaagggattattgtcacatactattctaaacttaat
tattgcaatgttgaacggggcagGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATAGCTTGACCCTCTCAAAGTTGCCCAAATTCCTGATTATTTGTTTGTAAATGGCAGTTTAGTTTTGTTAAACTGAATTGGAAATGGTAATTTCATTGTACATTGCCCTGTACATTTTGTATCTAACTCTAAATTAG
CTTGTGGTGTTTGATACACAACCCACTTCTTTTAATTATAAAATGCTCTTTCCATATTTGGAGCATAATGTACTTTTCTTTTGAAGTTGATAATATAAATAACTGTGGAGTTATAGACATTAAATGTAATGTGTAAGAAACTGATACTACAAAGGTAACTACTTGAGCGTTGTTGGGTTTGGATTGCAGTGGAAATGTGG
AGAATGGAAGTGAAGTTTGTAGTTGTGAATTGTACCAAAAAAACTACTGATTTCCTTAATCAGCAAAAATGGACAGCAGGTCCTAGTATCTGAGCTTGTGGAAAAGACAGATTCTCTGTATTTCTCTGCCCATTGGTG

Intron 4 GTTTTTATAG ... AATTTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.04	100	135	88.3
A2	67	97.3	143	83.7
A3	67.92	93.7	143.7	90.7
A4	78.46	52	124.3	88
A5	46.75	61.7	63.3	36.7
A6	47.42	525	530	281.3
A7	53.64	560.3	636	260
A8	51.37	592	650.3	332
A9	52.41	527.3	581	265.7
A10	53.18	429.7	482.7	217

>TRINITY_DN45572_c0_g5_i6 from sample A6 / Example spliced isoform

GCACCTCCAGAATCAGGAAGGGTGTTTTCTTACCATAAGAATGAAAAACATATATAGTGCAAAGAAACAATGGATGTGAAGGCTGAGTTCCTGAATGGAAAGGGTGATAATTTTGGAACTACTGAGTTCAGTTGGATTGATGATATCCCTATCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGT
ATATCTCCAAACTATTGCACCTACTGCATCAAAATATGGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATAGCTTGACCCTCTCAAAGGTGCCCAAATTCCTGATTATTTGTTTGTAAATGGCAGTTTAGTTTTGTTAAACTGAATTGGAAATGGTAATTTCATTGTACATTGCCCTG

>TRINITY_DN45572_c0_g5_i2 from sample A6 / Example unspliced isoform:

GCACCTCCAGAATCAGGAAGGGTGTTTTCTTACCATAAGAATGAAAAACATATATAGTGCAAAGAAACAATGGATGTGAAGGCTGAGTTCCTGAATGGAAAGGGTGATAATTTTGGAACTACTGAGTTCAGTTGGATTGATGATATCCCTATCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGT
ATATCTCCAAACTATTGCACCTACTGCATCAAAATATGgtttttataggattcaatgcttcttctgcaatttgtagaagattaatttgtcagGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATAGCTTGACCCTCTCAAAGGTGCCCAAATTCCTGATTATTTGTTTGTAAATGGCAGTTTAGTTTTGT
TAAACTGAATTGGAAATGGTAATTTCATTGTACATTGCCCTG

Intron 5 GTAAGAATCT ... TATATGCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60.85	287	446	126
A2	58.67	305	433	88
A3	57.18	292	390	130
A4	54.92	188	229	82
A5	59.78	148	220	59
A6	68.72	1125	2471	102
A7	64.39	1379	2493	129
A8	71.46	1314	3290	110
A9	68.19	1266	2714	118
A10	64.99	1063	1973	111

>TRINITY_DN45572_c0_g5_i4 from sample A6 / Example spliced isoform

GCACCTCCAGAATCAGGAAGGGTGTTTTCTTACCATAAGAATGAAAAACATATATAGTGCAAAGAAACAATGGATGTGAAGGCTGAGTTCCTGAATGGAAAGGGTGATAATTTTGGAACTACTGAGTTCAGTTGGATTGATGATATCCCTATCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGT
ATATCTCCAAACTATTGCACCTACTGCATCAAAATATGATAGTATCTCCCTTGGTAGCCTCAGTACCTGCAGGAGTGGTTTTGATGAGGGAGAAATTAGGCTTCAAGTTCACAACTGGGGTGCAACCTTTGCGATTTGCAAACTGGGATAGTGAAGATAAAATAACCTTCTGTATGAGTGGAAGGCAAGTAAATAACTAG
TTATGCCATAGGCGTTCTTTCAGTGAGAAACTCTGCCTGGGCACCGAACAGATTCCTCATCACTCTGCCCAACCGCCTTCCGCCATGCGTGTTTTTCAATTAACTCTTCTATCGCATGCCAGCTGTGTTGCGTGTCGCAGTTTGAAGATGGTAATTTCACTGTACATTTTATTTTGAACGATTATTGCACTGTAAATGGA
AGGTGTGTTGTGGTTATCCAACTTCCATTGCTTATGTGGGTACTAGGTTTGATTCTCACTGCCTGCTATATAGCAGTTCCAGTACCTGTGCCAAATCATAAATTTAACGTTCCTGATAACACCAGGGACCTTGAAGCTACCTTACAGGGATTACAGGGCTTTCAGTGAATATGATTTTGAATCTCAAATATCTAGGGTTT
CGTTGTTTTACAGGTTTTTATAGGATTCAATGCTTCTTCTGCAATTTGTAGAAGATTAATTTGTCAGG

>TRINITY_DN45572_c0_g5_i5 from sample A6 / Example unspliced isoform:

GCACCTCCAGAATCAGGAAGGGTGTTTTCTTACCATAAGAATGAAAAACATATATAGTGCAAAGAAACAATGGATGTGAAGGCTGAGTTCCTGAATGGAAAGGGTGATAATTTTGGAACTACTGAGTTCAGTTGGATTGATGATATCCCTATCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGT
ATATCTCCAAACTATTGCACCTACTGCATCAAAATATGgtaagaatctatgcaaaaatgtaaaacgagacctgctcttatttcttgttatttgtgttatatgataggaagaatagttatttgtacagtttttgtaatatatatcaaaaaaattgttgtactattttagattatgctgttaatgggctagctttcactgat
ttcactttgctcgtgcgagcatacttatagtgaatgcatacaaaattttagaatagagagtattataagaatcgtggtaaacagagcatttgaaagacttgaagaattttaattactaataacagtgaatcattctgggtcaatctgtcatactctcatgcactaaattatgatgtttctctgcatcattctcaaatgtt
gtttctcattgtgtttggattatagtgtggcccccacgaaaggagtattttgtatcgttgttcttttaaggcatcttgatttcaatctgtcaatgatcatgctatgccctttccataaaaaaatagctggatccacagaaagtccagaaagaatgcatgtatttgtttgtttttgtggatggttactttgaagttaaatc
taacttctgcttttaagttatttggatacgtcctaagttggaagttttcaatctcatgtatatgcaagATAGTATCTCCCTTGGTAGCCTCAGTACCTGCAGGAGTGGTTTTGATGAGGGAGAAATTAGGCTTCAAGTTCACAACTGGGGTGCAACCTTTGCGATTTGCAAACTGGGATAGTGAAGATAAAATAACCTTC
TGTATGAGTGGAAGGCAAGTAAATAACTAGTTATGCCATAGGCGTTCTTTCAGTGAGAAACTCTGCCTGGGCACCGAACAGATTCCTCATCACTCTGCCCAACCGCCTTCCGCCATGCGTGTTTTTCAATTAACTCTTCTATCGCATGCCAGCTGTGTTGCGTGTCGCAGTTTGAAGATGGTAATTTCACTGTACATTTT
ATTTTGAACGATTATTGCACTGTAAATGGAAGGTGTGTTGTGGTTATCCAACTTCCATTGCTTATGTGGGTACTAGGTTTGATTCTCACTGCCTGCTATATAGCAGTTCCAGTACCTGTGCCAAATCATAAATTTAACGTTCCTGATAACACCAGGGACCTTGAAGCTACCTTACAGGGATTACAGGGCTTTCAGTGAAT
ATGATTTTGAATCTCAAATATCTAGGGTTTCGTTGTTTTACAGGTTTTTATAGGATTCAATGCTTCTTCTGCAATTTGTAGAAGATTAATTTGTCAGGTAATGTTCGTCTCTGTGGAATTTGATTTGGAGTTTTTTTCCTTGTTTTATTGATAATTAGGGTGAAAAAGGTTCTACGGGTGCGCAATGTGTTTAATTCTGA
TGGTTAGGGTTTGGAGTGTTCACATGCTTGCTTGTATCTAGATTTTCACAGCTGGTATGCTAGAAGGGATTATTGTCACATACTATTCTAAACTTAATTATTGCAATGTTGAACGGGGCAGGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATAGCTTGACCCTCTCAAAGGTGCCCAAATTCCTGATTA
TTTGTTTGTAAATGGCAGTTTAGTTTTGTTAAACTGAATTGGAAATGGTAATTTCATTGTACATTGCCCTG

Intron 6 GTTAGTAGTT ... CTTCTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.35	16	26	27
A2	60.98	16	21	29
A3	67.86	18	42	34
A4	74.6	8	18	29
A5	61.29	12	14	24
A6	60.56	70	71	144
A7	59.74	63	76	111
A8	58.95	109	104	209
A9	60.69	68	84	126
A10	61.75	35	48	65

>TRINITY_DN102933_c0_g1_i5 from sample A4 / Example spliced isoform

ATGGGAAGGACTTAAACAAATCTAACATGACTAGAAGTGGAGGAGATGTTTTAGCACCTCCAGAATCAGGAAGGGTGTTTTTTCACCCTAAGAATGAAAAAGATTTACAGTGCAAAGAAACAATGGATATGAAGGCTGATTTCCCAAATGGCAAGGCTGATAAATTTGGAACTAGTGAGTTCAGTTGGATTGACGATATT
CCGAGCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGTATATCTCCAAACTATTGCACCTATTGCATCAAAATATGGTATTTGCAAGATAGTATCTCCCTTGGTAGCCTCAGTACCTGCAGGAGTGGTTTTGATGAAGGAGAAATTGGGCTTCAAGTTCACAACTCGGGTGCAACCTTTGAGATT
TGCAAACTGGGATACTGAAGATAAAATAACCTTCTCTATGAGTGGAAGAAACTATTCCTTCCGTGAATACGAGAGGATGGCAAATAAGGCATTTGCCCGCAAGTTCTCAACTGCTGCAAACTTGCCTAGCAAGTTTGTGGAGGAGGAATTTTGGCGTGAGATAACTTCTGGAAAGACACCAGCTGTTGAATATGCATGTG
ATATTGAAGGAAGTGCTTTCTCAGAATCACCAAGTGATCCACTTGGAACCAGCAAATGGAATTTAAAGGACGTGGCAAGACACCCAAGCTCTATATTGCGCCTTCTTGAAACTCCTATTCCGGGTGTCACTGATCCTATGCTATACATTGGAATGCTATTTAGCATGTTTGCATGGCATGTTGAAGATCACTATTTGTAC
AGCCTAAATTATCACCATTGTGGTGCACCCAAGACATGGTATGGTGTTCCTGGCAGTGCTGCTCATGATTTCGAAAATGTGGTTAGGGAGCATGTCTATGATCAGGAATTGCTCTTAGATGAAGCAGAAGGCGCAGTGTATGATTTTCTTATAGGGAAGACAACAATGTTCCCTCCAGATGTGTTATCTAAACATGGAGT
TCCTGTCTTCAAAGCTGTGCAAAAACCCGGAGAGTTTGTCATCACTTTTCCTCGATCTTATCATGCAGGCTTTAGTCATGGATTCAATTGTGGAGAGGCTGTGAACTTTGCTTTAATTGACTGGTTTCCATTTGGGGCGGCAGCATGTAAACGCTATGAGTTTCTTAACAGGGTGGCGCTTTTACCTCACGAGGAATTAC
TGTGTAAAGAAACAATGAATCTTGCCAGGACTAGGTTTTCTGAAGAGGGAAGGCATTCTTCTTTATCTGAGGAACCAGCAGGGCAACTATTTTTGAAGGTTGCATTTGTTAAGCTTATAAGGTTACAGCATCAGATGATTTGGTCACTAAAGAAGCTAGGAGCACAGACAAGTATTTCACTGGGACACATGAGCACCTTG
CGTTGCGGTCTCTGCAAGTGCTTATGTTATGTGGCATACATTAATTGTGAATGCTATACCCATCCAATGTGCCTCAATCATGTGAGGGAGATAAGAGAATGTAACTGTGGCAGTATGACTGTCTTTGTAAGAGAGGATTTTATGAAGATGGTTTCTGTAGCACAAAGATTTGAACAGGATGAGGGAATTCTTGAGGAAGT
GTTGGCAGATCTACCTGTAAAAGATCATACTGAAACGGATTACTTTGACTCCGAAGAAAGTTTGCTTAATTGCGACTTTGCCGAATATGTTCCATATTCTCATCTGGGTTTGGGAGAATATACTTCATTAAATAAGAATTCAAATGGTGAAAGTAGTAGCAGTTGTGAACCACTGGTTCATAGAAAACGGCAATCAGAAA
GCTCTCTATCTTCAGGAGTAGGTGGAGATCTACGTACATCTAACATTGTTCATGCAAAGGAATCATCTGCTGCTTCCAAACAGGGTGCTGCTGTACGGTCTTTAAAGAAGTTGAATGGCAAGGTCTATAGGAATTCAGAGGCTAAAATGGCTACTCAAAACATTTCCATTCCACGGAGCCAATGTGCAGTTGAATCAGAG
GACAAAAAGTTTAATCTGCTTTCCACTCCAGATGGACGGCATGTGCTGCTGATATCGGATATTCTATCTGCGATGAAAGCTGAAGTTTCATCTTCTGAGATCCTGAACAAGTGGAAGGCAAAGGCAAAGGCACTTTCCAAGGGGTTGGATCTCAAGGCACACCATGTTGATATCCAGTCAGTTTCTGAGCTTGACAAGAT
TGAGGCCAATGATCATTGGGGACTATCACTGAAGGGATGTATGCAGTTAAGAAAACTCCTAGAAGAACATGAAGTCCGTCTGCTGCAGAAACTGGTGCAGAAAACATTTGGGAATCTCTATGGTTCCACTAATGCTGGAGGCCTGGCTTTGAAATCTACAAAAGTAAATGGTGGAAAAGGCGAGAAAAGAGATTTTGATA
CCATGAACAAGACTGTTGATCTGAACACTCGTAAGATCTCAGGTGGTAAAAACACTGCTAATTTCTCAGAAGTATCCTCAAAGAATCCTACTCATGGTGGAAGAAGCAAGAAAAGAGATTTTGATACCATGAACAAGACTGCTGATGTGAGTGCTCATAAGATCTCAGGTAGCAAGGACAGTGCTAGTTTGCCAATAGTA
TCCTTAAAGAAGCCTGTATATGGTGGAAAAGGAACGAAAATAGATTTTGATACCATGAACAAGACTGCTGATGTGAAGACTTGTAAGATCTCAGGTGGTAAAGACAGTGTTAGTTTATCAGAAGTATCCTTAAAGAAGCCTTGTCCAGAATCCAAGATGAGAACTACATATCTTGACCAGTCTGTTATGAGAAAGCCTCA
AGAAGTAAAAGCTCAAGGTGACAGGCAAATGAAGAATGTCAACCAGAACTATATGGAAACATCTTCCTCATCTGCTGTAAACCAGCGACACAGATCTGTGCGCAGGAGGACTTTACAGTGTTCAAAAGAAGCAAAAGCAAGAAAGGATGACCAGTCAGAGGAAAAAGAACACCTGAATTTGTGGGTTAAAAATGAAAATG
TGAAATCCCAAGAGACAGACAAAGAGGCTTCAAAGAAGTTAACAATTTTATCAGAAGAGAGCCTTCAGGAGAATCCACTCTCAGCAAATGTTGTGGTGGAATCTGGCCAAGACTGTGGGTCAAAAGTGGGAAATAGAGAGAACCTTACAGCTGCTAAAAATAGAATTCGCATGCAGGGAAAACATGCTGTAGCCATACCA
GAGAAAGGAGCCGACCCCAGAGGTTCATCTGTACAGGATAGTGTTCTGCTAAAGCATGAACAGCTTGAACATACTGTTAGATCTGCTAAGGGCATAAAGATGAGGGAAAGAGAGTCTACCATACAATGTGACAGAAAGTCAAGTTCAAAGAAACCTAGTATAAAATTATCAAATGATGAAGAGGTACCTTCAGAAAATCA
AAGAGAAAATGGGAAAGTTAAACGACCTTTTGCAAAAGACATGAGCAGTGATAATTGTACGACTACTGAGAGAAAATCATCTTCTCAGATCAGCCGTCTGAAAATCAAAGGCCCATCTCCACCTATTGTTTCTGGAGGTCTTTGTGTTTTAGAGAACAATTTTCATCAAATTGACACAGATTGTGGCATAATGGCAGCTG
GAGATTCTTTGACAGAAGGATTTAATAGCAGTGTACCGAAGAGCAACAAGGAAGGGGGAATTTGCCTTCAGCCTCAGAATGCCGGGAAGAAAAGTAAAGTAAATATGCAAGGACAAATTGACATGGACAAAGGCAAGGGGGAGAAAGAAGAAGCCAGAGATTTAATGAATATTGATATCCCTAGAGCTGATTTTTATTCA
GAAGCAGGACTGACAGCTGTATCATTTTCAACTCTTGAAAATAGGGATAAAGATATATACAGTGCTAAAAAAGAGCAATTAAAAGAAAATACTTTCCAGCGTCTTCAGAGTGAAAATCATGAGGTGAGATCAACAGGGGAAAATCCAGGAGCTGTAAAAAGTACCGAAGCTGCCAACAATGCTATCTGGTACAAGAGCTG
TGATCTTGGCAATACTTTTAAGTTTTCGGATCATTACTCTTCTGTGGTCAAAGGAGGTCAAACTAGTAGTAGTTCAGGTTTCTCCAGTGGATTTTCAGCCATGTCACCTGAACTGAGAGTCTCTTTGGATTTTGGTTGTGGGAGTGGTATGCCGAATGACAGACACTGTATTAGTAAAGAAGAATTCTCACCTAAAAAAA
AGGCTTCTGGATCAGGCTGCATCAATGGCTCAGCTATGAAACAGGGGGAATTTCCAAGATCTGTAGATAATGAAAGAATTTCCATTTTGAAATTGTCAAGGAGAATTAGCTCTCCAGAGTTACGGTCAAAATCAATATCTTGTCATACTTTTGATACTGCTACATTTCCAGGAAAGCCTTCATCAAGTGCTCAGAAAGAG
TTGCTTCAAAGGGATCAGAGTCCACAGCTGCGAGGGGATAACAATTCAAGACAATCGGGTCATGAATATGACCAGTTGACTCCGGAGAAGCAATTTCACAGAATTGGATTTGAGCATGAAAATTCCTCTCAATATAAATGCCAGTATGAAGTCAGAGGCCAAGAGGTGAAACCATGTTCATTCAGAAACAAATGGAGGGA
TGTGGCTTCAACTTCAACCAAGGAGTGTCCAAGTCCAGAATCATTAATAACTGAAAACTATTTACCTGAAAAGCAAGTTGTAGATACTTGTACTGCTGATGGCCGTGATAAATTCAGATCAAGGGCCTATGATGGGTATGAACCATTTTGCAGGGACTCAGGTGCACAACAGCCAATTGAACCAACAAAAGAATTCAGTT
CAAGTTGGAAAAACACTGGAGCAGGTAAGAGCTTCACAAAACAAGCAGTACATTTTAACATGGAATATATAGGATTTGAAAACAAAAATAGTTCTTTCATGCCACAATCTCCTCGTGCTTCCAGTGTAGATTATTCTGAGGAAACAAAGACACTAAAGATAATGCCAGCTGATGAAAATTCCTTCCCTGTACAGCCTGAT
CTGAAATATAATCTTTGTCAAAAAGAAGATTCTCCACAATCTGTTAATAGAAAGGGGCAAAAGATGAACCATAATCATTGGAAAGATATGTCCAAATGTCAGATGAGTCCTTTTGGACCTCAAGTTGGTTCATATGTTTCTAGGGACAACCATGTCCCGACTGTAAGCAGAGAGAAGTGGCCAAGTCATCAGAAATGGAT
TGTAAATGAAAACAAGGATAATGGAATGTGTAAAACTGAAACTCCTGGAAATTTGCATCCAAACCTACAGCCAAAATATTCAATAGACCACTCAGCTCCACAGTTTGATGCTGGTGCAGGTCAAGAATTTGCTATAGAAAATTTTGCAGTTTTTGATCACGGAAAAGAATCCCAGGCTCAATCAGATTTTCATCAGGGCA
ATGCCTTATGGGCAGATACAAGAACACCTTTATTAGAAGATGAAATGGAATTTGCCATTGACATGCAGCCTAATAATAGGAGCCGGCAAAGCCACAATAAGATGTCAGTGGCAGTGGACTCAGAATCACAGCAGATGTGCACATCAGAACATGGGTACTGGCCATCGTTTGTGCACACTCCCAGAAAGCAAGAACAGTTA
GCTCGTCAGAAGCTACTAATTGAAAAGTGGGGTAGAGCATGATCTCAATGTGATGATGCACTGGTTGACTTCAGCAATGACACAATTGCCCTGCTGGACATAGTAGAGGGTGTAATCATCTTTCACATCATTCTCAGTCAAATGATATTGTAAGAATTTCTGCAGATCTGTTAACAAGCTGTACTGGCCACTGGTCGAAC
TGACTATACTGTACAATATAATTATTTGATTCTTTTATTTTTGCTGAGAAGCCCAATGGGTGCTATCTCCATACATTGGTTGTATCCATTGAATTTTAGGTGATTCTCAGGCCCAAAAATTTCTTTATTCTCTGAGTGCAGCATCACTTCGATGCTAATAACTAGAGAATCAGACTGACGTACAGTACTTCTGGGTTGTC
AAGTGCCTACCATGACTGACACTAAAGCT

>TRINITY_DN102933_c0_g1_i10 from sample A4 / Example unspliced isoform:

ATGGGAAGGACTTAAACAAATCTAACATGACTAGAAGTGGAGGAGATGTTTTAGCACCTCCAGAATCAGGAAGGGTGTTTTTTCACCCTAAGAATGAAAAAGATTTACAGTGCAAAGAAACAATGGATATGAAGGCTGATTTCCCAAATGGCAAGGCTGATAAATTTGGAACTAGTGAGTTCAGTTGGATTGACGATATT
CCGAGCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGTATATCTCCAAACTATTGCACCTATTGCATCAAAATATGGTATTTGCAAGATAGTATCTCCCTTGGTAGCCTCAGTACCTGCAGGAGTGGTTTTGATGAAGGAGAAATTGGGCTTCAAGTTCACAACTCGGGTGCAACCTTTGAGATT
TGCAAACTGGGATACTGAAGATAAAATAACCTTCTCTATGAGTGGAAGAAACTATTCCTTCCGTGAATACGAGAGGATGGCAAATAAGGCATTTGCCCGCAAGTTCTCAACTGCTGCAAACTTGCCTAGCAAGTTTGTGGAGGAGGAATTTTGGCGTGAGATAACTTCTGGAAAGACACCAGCTGTTGAATATGCATGTG
ATATTGAAGGAAGTGCTTTCTCAGAATCACCAAGTGATCCACTTGGAACCAGCAAATGGAATTTAAAGGACGTGGCAAGACACCCAAGCTCTATATTGCGCCTTCTTGAAACTCCTATTCCGGGTGTCACTGATCCTATGCTATACATTGGAATGCTATTTAGCATGTTTGCATGGCATGTTGAAGATCACTATTTGTAC
AGCCTAAATTATCACCATTGTGGTGCACCCAAGACATGGTATGGTGTTCCTGGCAGTGCTGCTCATGATTTCGAAAATGTGGTTAGGGAGCATGTCTATGATCAGGAATTGCTCTTAGATGAAGCAGAAGGCGCAGTGTATGATTTTCTTATAGGGAAGACAACAATGTTCCCTCCAGATGTGTTATCTAAACATGGAGT
TCCTGTCTTCAAAGCTGTGCAAAAACCCGGAGAGTTTGTCATCACTTTTCCTCGATCTTATCATGCAGGCTTTAGTCATGGATTCAATTGTGGAGAGGCTGTGAACTTTGCTTTAATTGACTGGTTTCCATTTGGGGCGGCAGCATGTAAACGCTATGAGTTTCTTAACAGGGTGGCGCTTTTACCTCACGAGGAATTAC
TGTGTAAAGAAACAATGAATCTTGCCAGGACTAGGTTTTCTGAAGAGGGAAGGCATTCTTCTTTATCTGAGGAACCAGCAGGGCAACTATTTTTGAAGGTTGCATTTGTTAAGCTTATAAGGTTACAGCATCAGATGATTTGGTCACTAAAGAAGCTAGGAGCACAGACAAGTATTTCACTGGGACACATGAGCACCTTG
CGTTGCGGTCTCTGCAAGTGCTTATGTTATGTGGCATACATTAATTGTGAATGCTATACCCATCCAATGTGCCTCAATCATGTGAGGGAGATAAGAGAATGTAACTGTGGCAGTATGACTGTCTTTGTAAGAGAGGATTTTATGAAGATGGTTTCTGTAGCACAAAGATTTGAACAGGATGAGGGAATTCTTGAGGAAGT
GTTGGCAGATCTACCTGTAAAAGATCATACTGAAACGGATTACTTTGACTCCGAAGAAAGTTTGCTTAATTGCGACTTTGCCGAATATGTTCCATATTCTCATCTGGGTTTGGGAGAATATACTTCATTAAATAAGAATTCAAATGGTGAAAGTAGTAGCAGTTGTGAACCACTGGTTCATAGAAAACGGCAATCAGAAA
GCTCTCTATCTTCAGGAGTAGGTGGAGATCTACGTACATCTAACATTGTTCATGCAAAGGAATCATCTGCTGCTTCCAAACAGGGTGCTGCTGTACGGTCTTTAAAGAAGTTGAATGGCAAGGTCTATAGGAATTCAGAGGCTAAAATGGCTACTCAAAACATTTCCATTCCACGGAGCCAATGTGCAGTTGAATCAGAG
GACAAAAAGTTTAATCTGCTTTCCACTCCAGATGGACGGCATGTGCTGCTGATATCGGATATTCTATCTGCGATGAAAGCTGAAGTTTCATCTTCTGAGATCCTGAACAAGTGGAAGGCAAAGGCAAAGGCACTTTCCAAGGGGTTGGATCTCAAGGCACACCATGTTGATATCCAGTCAGTTTCTGAGCTTGACAAGAT
TGAGGCCAATGATCATTGGGGACTATCACTGAAGGGATGTATGCAGTTAAGAAAACTCCTAGAAGAACATGAAGTCCGTCTGCTGCAGAAACTGGTGCAGAAAACATTTGGGAATCTCTATGGTTCCACTAATGCTGGAGGCCTGGCTTTGAAATCTACAAAAGTAAATGGTGGAAAAGGCGAGAAAAGAGATTTTGATA
CCATGAACAAGACTGTTGATCTGAACACTCGTAAGATCTCAGGTGGTAAAAACACTGCTAATTTCTCAGAAGTATCCTCAAAGAATCCTACTCATGGTGGAAGAAGCAAGAAAAGAGATTTTGATACCATGAACAAGACTGCTGATGTGAGTGCTCATAAGATCTCAGGTAGCAAGGACAGTGCTAGTTTGCCAATAGTA
TCCTTAAAGAAGCCTGTATATGGTGGAAAAGGAACGAAAATAGATTTTGATACCATGAACAAGACTGCTGATGTGAAGACTTGTAAGATCTCAGGTGGTAAAGACAGTGTTAGTTTATCAGAAGTATCCTTAAAGAAGCCTTGTCCAGAATCCAAGATGAGAACTACATATCTTGACCAGTCTGTTATGAGAAAGCCTCA
AGAAGTAAAAGCTCAAGGTGACAGGCAAATGAAGAATGTCAACCAGAACTATATGGAAACATCTTCCTCATCTGCTGTAAACCAGCGACACAGATCTGTGCGCAGGAGGACTTTACAGTGTTCAAAAGAAGCAAAAGCAAGAAAGGATGACCAGTCAGAGGAAAAAGAACACCTGAATTTGTGGGTTAAAAATGAAAATG
TGAAATCCCAAGAGACAGACAAAGAGGCTTCAAAGAAGTTAACAATTTTATCAGAAGAGAGCCTTCAGGAGAATCCACTCTCAGCAAATGTTGTGGTGGAATCTGGCCAAGACTGTGGGTCAAAAGTGGGAAATAGAGAGAACCTTACAGCTGCTAAAAATAGAATTCGCATGCAGGGAAAACATGCTGTAGCCATACCA
GAGAAAGGAGCCGACCCCAGAGGTTCATCTGTACAGGATAGTGTTCTGCTAAAGCATGAACAGCTTGAACATACTGTTAGATCTGCTAAGGGCATAAAGATGAGGGAAAGAGAGTCTACCATACAATGTGACAGAAAGTCAAGTTCAAAGAAACCTAGTATAAAATTATCAAATGATGAAGAGGTACCTTCAGAAAATCA
AAGAGAAAATGGGAAAGTTAAACGACCTTTTGCAAAAGACATGAGCAGTGATAATTGTACGACTACTGAGAGAAAATCATCTTCTCAGATCAGCCGTCTGAAAATCAAAGGCCCATCTCCACCTATTGTTTCTGGAGGTCTTTGTGTTTTAGAGAACAATTTTCATCAAATTGACACAGATTGTGGCATAATGGCAGCTG
GAGATTCTTTGACAGAAGGATTTAATAGCAGTGTACCGAAGAGCAACAAGGAAGGGGGAATTTGCCTTCAGCCTCAGAATGCCGGGAAGAAAAGTAAAGTAAATATGCAAGGACAAATTGACATGGACAAAGGCAAGGGGGAGAAAGAAGAAGCCAGAGATTTAATGAATATTGATATCCCTAGAGCTGATTTTTATTCA
GAAGCAGGACTGACAGCTGTATCATTTTCAACTCTTGAAAATAGGGATAAAGATATATACAGTGCTAAAAAAGAGCAATTAAAAGAAAATACTTTCCAGCGTCTTCAGAGTGAAAATCATGAGGTGAGATCAACAGGGGAAAATCCAGGAGCTGTAAAAAGTACCGAAGCTGCCAACAATGCTATCTGGTACAAGAGCTG
TGATCTTGGCAATACTTTTAAGTTTTCGGATCATTACTCTTCTGTGGTCAAAGGAGGTCAAACTAGTAGTAGTTCAGGTTTCTCCAGTGGATTTTCAGCCATGTCACCTGAACTGAGAGTCTCTTTGGATTTTGGTTGTGGGAGTGGTATGCCGAATGACAGACACTGTATTAGTAAAGAAGAATTCTCACCTAAAAAAA
AGGCTTCTGGATCAGGCTGCATCAATGGCTCAGCTATGAAACAGGGGGAATTTCCAAGATCTGTAGATAATGAAAGAATTTCCATTTTGAAATTGTCAAGGAGAATTAGCTCTCCAGAGTTACGGTCAAAATCAATATCTTGTCATACTTTTGATACTGCTACATTTCCAGGAAAGCCTTCATCAAGTGCTCAGAAAGAG
TTGCTTCAAAGGGATCAGAGTCCACAGCTGCGAGGGGATAACAATTCAAGACAATCGGGTCATGAATATGACCAGTTGACTCCGGAGAAGCAATTTCACAGAATTGGATTTGAGCATGAAAATTCCTCTCAATATAAATGCCAGTATGAAGTCAGAGGCCAAGAGGTGAAACCATGTTCATTCAGAAACAAATGGAGGGA
TGTGGCTTCAACTTCAACCAAGGAGTGTCCAAGTCCAGAATCATTAATAACTGAAAACTATTTACCTGAAAAGCAAGTTGTAGATACTTGTACTGCTGATGGCCGTGATAAATTCAGATCAAGGGCCTATGATGGGTATGAACCATTTTGCAGGGACTCAGGTGCACAACAGCCAATTGAACCAACAAAAGAATTCAGTT
CAAGTTGGAAAAACACTGGAGCAGGTAAGAGCTTCACAAAACAAGCAGTACATTTTAACATGGAATATATAGGATTTGAAAACAAAAATAGTTCTTTCATGCCACAATCTCCTCGTGCTTCCAGTGTAGATTATTCTGAGGAAACAAAGACACTAAAGATAATGCCAGCTGATGAAAATTCCTTCCCTGTACAGCCTGAT
CTGAAATATAATCTTTGTCAAAAAGAAGATTCTCCACAATCTGTTAATAGAAAGGGGCAAAAGATGAACCATAATCATTGGAAAGATATGTCCAAATGTCAGATGAGTCCTTTTGGACCTCAAGTTGGTTCATATGTTTCTAGGGACAACCATGTCCCGACTGTAAGCAGAGAGAAGTGGCCAAGTCATCAGAAATGGAT
TGTAAATGAAAACAAGGATAATGGAATGTGTAAAACTGAAACTCCTGGAAATTTGCATCCAAACCTACAGCCAAAATATTCAATAGACCACTCAGCTCCACAGTTTGATGCTGGTGCAGGTCAAGAATTTGCTATAGAAAATTTTGCAGTTTTTGATCACGGAAAAGAATCCCAGGCTCAATCAGATTTTCATCAGGGCA
ATGCCTTATGGGCAGATACAAGAACACCTTTATTAGAAGATGAAATGGAATTTGCCATTGACATGCAGCCTAATAATAGGAGCCGGCAAAGCCACAATAAGATGTCAGTGGCAGTGGACTCAGAATCACAGCAGATGTGCACATCAGAACATGGGTACTGGCCATCGTTTGTGCACACTCCCAGAAAGCAAGAACAGTTA
GCTCGTCAGAAGCTACTAATTGAAAAGTGGGGTAGAGCATGATCTCAATGTGATGATGCACTGGTTGACTTCAGCAATGACACAgttagtagttaccaatcattgacctgattctataagcaatttttttcccacttgcaatgactgcctgattacagggccaagaaaactagcattggttttgttgctggcccaggttc
aagtcaggtaaattgtgtgggactaacattaggacttgtcttctttcagATTGCCCTGCTGGACATAGTAGAGGGTGTAATCATCTTTCACATCATTCTCAGTCAAATGATATTGTAAGAATTTCTGCAGATCTGTTAACAAGCTGTACTGGCCACTGGTCGAACTGACTATACTGTACAATATAATTATTTGATTCTTT
TATTTTTGCTGAGAAGCCCAATGGGTGCTATCTCCATACATTGGTTGTATCCATTGAATTTTAGGTGATTCTCAGGCCCAAAAATTTCTTTATTCTCTGAGTGCAGCATCACTTCGATGCTAATAACTAGAGAATCAGACTGACGTACAGTACTTCTGGGTTGTCAAGTGCCTACCATGACTGACACTAAAGCT

Intron 7 GTAAGAATCT ... AACGGGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.64	244	446	97.5
A2	63.03	254	433	93.5
A3	61.22	247	390	96
A4	62.74	136	229	60
A5	59.95	147	220	53.5
A6	66.28	1257	2471	189
A7	62.99	1465	2493	193
A8	69.23	1462	3290	267
A9	66.72	1354	2714	187
A10	63.85	1117	1973	156.5

>TRINITY_DN45572_c0_g5_i6 from sample A6 / Example spliced isoform

GCACCTCCAGAATCAGGAAGGGTGTTTTCTTACCATAAGAATGAAAAACATATATAGTGCAAAGAAACAATGGATGTGAAGGCTGAGTTCCTGAATGGAAAGGGTGATAATTTTGGAACTACTGAGTTCAGTTGGATTGATGATATCCCTATCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGT
ATATCTCCAAACTATTGCACCTACTGCATCAAAATATGGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATAGCTTGACCCTCTCAAAGGTGCCCAAATTCCTGATTATTTGTTTGTAAATGGCAGTTTAGTTTTGTTAAACTGAATTGGAAATGGTAATTTCATTGTACATTGCCCTG

>TRINITY_DN45572_c0_g5_i5 from sample A6 / Example unspliced isoform:

GCACCTCCAGAATCAGGAAGGGTGTTTTCTTACCATAAGAATGAAAAACATATATAGTGCAAAGAAACAATGGATGTGAAGGCTGAGTTCCTGAATGGAAAGGGTGATAATTTTGGAACTACTGAGTTCAGTTGGATTGATGATATCCCTATCTGTCCAGTGTTCTACCCCACAAAGTAGGAGTTTGAAGATCCATCAGT
ATATCTCCAAACTATTGCACCTACTGCATCAAAATATGgtaagaatctatgcaaaaatgtaaaacgagacctgctcttatttcttgttatttgtgttatatgataggaagaatagttatttgtacagtttttgtaatatatatcaaaaaaattgttgtactattttagattatgctgttaatgggctagctttcactgat
ttcactttgctcgtgcgagcatacttatagtgaatgcatacaaaattttagaatagagagtattataagaatcgtggtaaacagagcatttgaaagacttgaagaattttaattactaataacagtgaatcattctgggtcaatctgtcatactctcatgcactaaattatgatgtttctctgcatcattctcaaatgtt
gtttctcattgtgtttggattatagtgtggcccccacgaaaggagtattttgtatcgttgttcttttaaggcatcttgatttcaatctgtcaatgatcatgctatgccctttccataaaaaaatagctggatccacagaaagtccagaaagaatgcatgtatttgtttgtttttgtggatggttactttgaagttaaatc
taacttctgcttttaagttatttggatacgtcctaagttggaagttttcaatctcatgtatatgcaagatagtatctcccttggtagcctcagtacctgcaggagtggttttgatgagggagaaattaggcttcaagttcacaactggggtgcaacctttgcgatttgcaaactgggatagtgaagataaaataaccttc
tgtatgagtggaaggcaagtaaataactagttatgccataggcgttctttcagtgagaaactctgcctgggcaccgaacagattcctcatcactctgcccaaccgccttccgccatgcgtgtttttcaattaactcttctatcgcatgccagctgtgttgcgtgtcgcagtttgaagatggtaatttcactgtacatttt
attttgaacgattattgcactgtaaatggaaggtgtgttgtggttatccaacttccattgcttatgtgggtactaggtttgattctcactgcctgctatatagcagttccagtacctgtgccaaatcataaatttaacgttcctgataacaccagggaccttgaagctaccttacagggattacagggctttcagtgaat
atgattttgaatctcaaatatctagggtttcgttgttttacaggtttttataggattcaatgcttcttctgcaatttgtagaagattaatttgtcaggtaatgttcgtctctgtggaatttgatttggagtttttttccttgttttattgataattagggtgaaaaaggttctacgggtgcgcaatgtgtttaattctga
tggttagggtttggagtgttcacatgcttgcttgtatctagattttcacagctggtatgctagaagggattattgtcacatactattctaaacttaattattgcaatgttgaacggggcagGAATTGATCCTGTGCTTATTTGCTCCAGTGAATTGCAATAATAGCTTGACCCTCTCAAAGGTGCCCAAATTCCTGATTA
TTTGTTTGTAAATGGCAGTTTAGTTTTGTTAAACTGAATTGGAAATGGTAATTTCATTGTACATTGCCCTG

Intron 8 GTTTTTATAG ... CCTCTCAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	63.93	143.5	203	82.5
A2	62.64	153	207.5	80
A3	61.8	158.5	212.5	98
A4	64.97	110.5	178.5	93
A5	64.46	72.5	94	26
A6	63.72	615.5	727	140
A7	58.63	822	932.5	227
A8	58.17	823	929.5	278.5
A9	58.05	740	822	211.5
A10	61.05	614.5	714	157.5

>TRINITY_DN123593_c1_g2_i3 from sample A2 / Example spliced isoform

GGAGTTTGAAGATCCATCAGTATATCTCCAAACTATTGCACCTACTGCATCAAAATATGTGGAAATGTGGAGAATGGAAGTGAAGTTTGTAGTTGTGAATTGTACCAAAAAAACTACTGATTTCCTTAATCAGCAAAAATGGACAGCAGGTCCTAGTATCTGAGCTTGTGGAAAAGACAGATTCTCTGTATTTCTCTGCC
CATTGGTGTCCCCC

>TRINITY_DN123593_c1_g2_i1 from sample A2 / Example unspliced isoform:

GGAGTTTGAAGATCCATCAGTATATCTCCAAACTATTGCACCTACTGCATCAAAATATGgtttttataggattcaatgcttcttctgcaatttgtagaagattaatttgtcaggaattgatcctgtgcttatttgctccagtgaattgcaataatagcttgaccctctcaaagTGGAAATGTGGAGAATGGAAGTGAAGT
TTGTAGTTGTGAATTGTACCAAAAAAACTACTGATTTCCTTAATCAGCAAAAATGGACAGCAGGTCCTAGTATCTGAGCTTGTGGAAAAGACAGATTCTCTGTATTTCTCTGCCCATTGGTGTCCCCC