sp|O24467|LE14B_PRUAR ----> LEC14B homolog

Intron 1 GTATCTTTCT ... ATTTTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20	180	0	45
A2	25.18	104	3	35
A3	25.4	94	5	32
A4	18.18	72	0	16
A5	19.35	25	0	6
A6	24.53	486	4	158
A7	24.28	552	1	177
A8	22.94	551	0	164
A9	20.92	514	0	136
A10	20.22	367	0	93

>TRINITY_DN36018_c0_g2_i5 from sample A6 / Example spliced isoform

ATTCCACCGAACCTCCCCGCTTACAACACAAATTCGCACGCAAAATCCCACGTCACGGATTTACTTGTGAAGTGAAAATGTGGCTTATTGTTGTTAAGAACAGAAGTGACCTTCCTCAATTAGGAAGACCATTGTTGCAACAGAAAGTATTGTGGGTTCGGGGATCGAATCTCTCAGGGGTCTCGTTTCAGGAATATGAG
CATGGTTAGGGGTCCTATGAGGTACCCCGGAGTTTACTTAGCTATGATTAGCTAAGTCATTTGAATCGTGTTTCCCTGTGCAAACAACAGAAATGGTGGGTCATGGTAATCGAAATACTAGTTTTACACGTGGAGCCATTCATCATTCTGGAAGGATTGGGATGCGCTTAGTAGATATTGAAGGTCAAGAAGCTTCACCA
AGCACAAGGAACAGAGCTGCAAACACAAGACAAAGGGAGACAATGGGCAACTCTCAAAATCATCATTTGGACACAGATGATAACAGTGACAGAAATGATGAAGGTCAAAATACAGATGATGCTCTTGAAGAATCCGCCAGACTTTACCATGAACTTGCACATATAACAAAACTGGGGTCAGCACCAAATGTTATGCTATC
CAGAATAAATGGTAAGAGCCAGAATGGCTATGTTTCAACATTATCTATGTTGGCAGGAAGAGAAGCCAATATTTCTGGGAGAGGGAAATTTAGTGTAGCTGATTGCCGTCATGTCGCAAGTAGATATTTGCCAATTTGTGGCCCAGCTATAATGGAGAGGATGAGGAGTAGAGCATATGTTGGACAATTTTCAGCAGATG
GCTCTCTTTTTGTCACAGGATTTCAGGATCATCGGATAAGAATATACAATGTGGAAAATAGATGGAGAGTTCAAAAGGAAGTGCGTGCTAGAAACTTGCGTTGGACAATCACTGACACTTCTCTTTCACCTGATCAGCGTTACCTTGTGTATGCTACTATCTCTCCTATTGTCCATATCGTGAATGTTGGAAGCAGTATC
ACAGAATCCTTAGCAAACGTCACAGAAATTCACGAGGGGTTAGACTTCTCTAAGTATGAAGAAGAATGTTCATTTGGACTTTTCTGTGTTAAGTTTTCAACCGATGGACGAGAACTTGTTGCTGGCGGCAATGACAAATCTATATATGTTTATGATCTTGAAGCGAGCAAGCGTTCTTTAAAGATAGTGGCTCATGAGGA
TGATGTGAATACAGTATCATTCGCAGATGAGACTAGCCACTTGATCTACTCTGGAAGCGATGATAGCTATTGCAAGGTCTGGGATAGGCGATGCCTTACATCCAGGGCACAGCCTGCTGGAACTCTAGTTGGGCATTTGGAAGGCATTACTTTCATTGATAGTCGTGGAGATGGACGCTATTTTATATCTAATGGAAAAG
ACCAGACAACAAAGCTGTGGGATATTCGCAAAATGACTGCTGGAAATCCTAGGATAGAGTCAATGAGGTTACCTGTCGATCAATGGGATTATCGTTGGATGGAGTATCCAGCAACTAGGAAGAATGTTAAGCATCCACATGACCAGTCTGTGATGACGTACAGGGGACATGCTGTCTTGCAGACTCTTGTCCGCTGTTAC
TTTTCTCCATCCTTCAGCACTGCCCAGAAATACATCTACACTGGATCCCATGATGGCTGTGTTTATATTTATGATGTGGTAACTGGGAACCTCATTAAGAAACTCGAGTATCATACTTCCGCTGTGAGGGACTGCAGCTGGCATCCATTATATCCAACTCTTGTGAGCTGTTCATGGGATGGGATTGTTGCCAAATGGGA
CCATTTTACTAGCGAGGTAGAACAGCCTAGAAGAGCAAAGAATCGCAGAAGCCGCATCCTGTGACTGACATATTGAAAGAATTAAATATACTGTGGGAAAGTACACATACTAGTGTCTATTATCTTGGGGAAATTTTAGTGCTCCAATTAAGCTATCAGTTAGAGATCATGAGCTGAAATTCAAGTGACTGGAAAGCTGA
TGTTACCAATTTATTCATATCTTGGAAAGCTGATGTTACCAATTTATTCATATCTTGGAAAGCTGCATCACCATAAAGCTAATGATGCTAAAGATCTCCCCTCCTTTACTGTCTGATCAGCAGATGGGAGGGTTGGTGAAGCTGTTATTCCTTGTCTTTTTATTCCCTCTTTATTCCATTTTTTTTTATAAACTTCAATG
GTTGTTATTCTGGCTTTAAAGAAGCCAATCATTTGATAGGGTGCTACAGTAGTCCGCCTTGAATCTTTCTTTGTATGTGTTTGGGGCCTGAAACCATCATTATTTTCATTATATATATGTATATATGTTTTTCATGACTACAAAAA

>TRINITY_DN36018_c0_g2_i6 from sample A6 / Example unspliced isoform:

CTAATTTGTTTTGCATATGGAATTCTAGCCCCCAGGTTGAGTTAAACACATGTTTTGCTTATTATCTAATCCTTTCAACCTCAGCTTCAGCATAAATCTGCCCAGAACATGAATGACAGTTATGTGCAGGTTGGAACCATGAATTTGCCTGGAGCAACGAAGTGACAGTTATGGGCTGGTTGAAACCATGAATTCATTGT
TCCAACCTACCCATAACTGTATCTTCATGGTTCCAGATAGATTTCTTTATGATGCTCAATTATTTCCTTGAAATAGTATTTAGAATCTCGTAAAATTTAGTATCATCAAAATTTTCTGTGATTCCTGCCCAATTTACTGTTTTGGACAAATAGAAAACAAAAGACTAGAAGCTAAAAGAATGACATGCAGAATGGCAGAA
TGTACCTCTACAGTTTCAAGTAACAATAAGAAACATTTAAAGAGAATTAAATAGAGCATTGCATCTTACAGTATTTTTCCTTCCTGCTTTTGTAGCACTGCCCAGAAATACATCTACACTGGATCCCATGATGGCTGTGTTTATATTTATGATGTGgtatctttctgaaccatttacttcttccctgattacagaaagat
cttcactgctttgcttaaactaaggtttgcatactcacatattttctctctagatcagatttttacagtcacatatgatggaatcattttcacagGTAACTGGGAACCTCATTAAGAAACTCGAGTATCATACTTCCGCTGTGAGGGACTGCAGCTGGCATCCATTATATCCAACTCTTGTGAGCTGTTCATGGGATGGG
ATTGTTGCCAAATGGGACCATTTTACTAGCGAGGTAGAACAGCCTAGAAGAGCAAAGAATCGCAGAAGCCGCATCCTGTGACTGACATATTGAAAGAATTAAATATACTGTGGGAAAGTACACATACTAGTGTCTATTATCTTGGGGAAATTTTAGTGCTCCAATTAAGCTATCAGTTAGAGATCATGAGCTGAAATTCA
AGTGACTGGAAAGCTGATGTTACCAATTTATTCATATCTTGGAAAGCTGATGTTACCAATTTATTCATATCTTGGAAAGCTGCATCACCATAAAGCTAATGATGCTAAAGATCTCCCCTCCTTTACTGTCTGATCAGCAGATGGGAGGGTTGGTGAAGCTGTTATTCCTTGTCTTTTTATTCCCTCTTTATTCCATTTTT
TTTTATAAACTTCAATGGTTGTTATTCTGGCTTTAAAGAAGCCAATCATTTGATAGGGTGCTACAGTAGTCCGCCTTGAATCTTTCTTTGTATGTGTTTGGGGCCTGAAACCATCATTATTTTCATTATATATATGTATATATGTTTTTCATGACTACAAAAA

Intron 2 GTATGTCTCT ... ATCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.55	184	87	46
A2	24.66	110	44	28
A3	29.66	115	62	35
A4	22.54	122	34	37
A5	16.85	37	10	5
A6	20.33	194	39	60
A7	20.94	287	91	61
A8	20.13	252	56	71
A9	21.26	287	90	65
A10	19.92	205	64	38

>TRINITY_DN112379_c3_g1_i2 from sample A3 / Example spliced isoform

CCGAACCTCCCCGCTTACAACACAAATTCGCACGCAAAATCCCACGTCACGGATTTACTTGTGAAGTGAAAATGTGGCTTATTGTTGTTAAGAACAGAAGTGACCTTCCTCAATTAGGAAGACCATTGTTGCAACAGAAAGTATTGTGGGTTCGGGGATCGAATCTCTCAGGGGTCTCGTTTCAGGAATATGAGCATGGT
TAGGGGTCCTATGAGGTACCCCGGAGTTTACTTAGCTATGATTAGCTAAGTCATTTGAATCGTGTTTCCCTGTGCAAACAACAGAAATGGTGGGTCATGGTAATCGAAATACTAGTTTTACACGTGGAGCCATTCATCATTCTGGAAGGATTGGGATGCGCTTAGTAGATATTGAAGGTCAAGAAGCTTCACCAAGCACA
AGGAACAGAGCTGCAAACACAAGACAAAGGGAGACAATGGGCAACTCTCAAAATCATCATTTGGACACAGATGATAACAGTGACAGAAATGATGAAGGTCAAAATACAGATGATGCTCTTGAAGAATCCGCCAGACTTTACCATGAACTTGCACATATAACAAAACTGGGGTCAGCACCAAATGTTATGCTATCCAGAAT
AAATGGTAAGAGCCAGAATGGCTATGTTTCAACATTATCTATGTTGGCAGGAAGAGAAGCCAATATTTCTGGGAGAGGGAAATTTAGTGTAGCTGATTGCCGTCATGTCGCAAGTAGATATTTGCCAATTTGTGGCCCAGCTATAATGGAGAGGATGAGGAGTAGAGCATATGTTGGACAATTTTCAGCAGATGGCTCTC
TTTTTGTCACAGGATTTCAGGATCATCGGATAAGAATATACAATGTGGAAAATAGATGGAGAGTTCAAAAGGAAGTGCGTGCTAGAAACTTGCGTTGGACAATCACTGACACTTCTCTTTCACCTGATCAGCGTTACCTTGTGTATGCTACTATCTCTCCTATTGTCCATATCGTGAATGTTGGAAGCAGTATCACAGAA
TCCTTAGCAAACGTCACAGAAATTCACGAGGGGTTAGACTTCTCTAAGTATGAAGAAGAATGTTCATTTGGACTTTTCTGTGTTAAGTTTTCAACCGATGGACGAGAACTTGTTGCTGGCGGCAATGACAAATCTATATATGTTTATGATCTTGAAGCGAGCAAGCGTTCTTTAAAGATAGTGGCTCATGAGGATGATGT
GAATACAGTATCATTCGCAGATGAGACTAGCCACTTGATCTACTCTGGAAGCGATGATAGCTATTGCAAGGTCTGGGATAGGCGATGCCTTACATCCAGGGCACAGCCTGCTGGAACTCTAGTTGGGCATTTGGAAGGCATTACTTTCATTGATAGTCGTGGAGATGGACGCTATTTTATATCTAATGGAAAAGACCAGA
CAACAAAGCTGTGGGATATTCGCAAAATGACTGCTGGAAATCCTAGGATAGAGTCAATGAGGTTACCTGTCGATCAATGGGATTATCGTTGGATGGAGTATCCAGCAACTAGGAAGAATGTTAAGCATCCACATGACCAGTCTGTGATGACGTACAGGGGACATGCTGTCTTGCAGACTCTTGTCCGCTGTTACTTTTCT
CCATCCTTCAGCACTGCCCAGAAATACATCTACACTGGATCCCATGATGGCTGTGTTTATATTTATGATGTGGTAACTGGGAACCTCATTAAGAAACTCGAGTATCATACTTCCGCTGTGAGGGACTGCAGCTGGCATCCATTATATCCAACTCTTGTGAGCTGTTCATGGGATGGGATTGTTGCCAAATGGGACCATTT
TACTAGCGAGGTAGAACAGCCTAGAAGAGCAAAGAATCGCAGAAGCCGCATCCTGTGACTGACATATTGAAAGAATTAAATATACTGTGGGAAAGTACACATACTAGTGTCTATTATCTTGGGGAAATTTTAGTGCTCCAATTAAGCTATCAGTTAGAGATCATGAGCTGAAATTCAAGTGACTGGAAAGCTGATGTTAC
CAATTTATTCATATCTTGGAAAGCTGATGTTACCAATTTATTCATATCTTGGAAAGCTGCATCACCATAAAGCTAATGATGCTAAAGATCTCCCCTCCTTTACTGTCTGATCAGCAGATGGGAGGGTTGGTGAAGCTGTTATTCCTTGTCTTTTTATTCCCTCTTTATTCCATTTTTTTTTATAAACTTCAATGGTTGTT
ATTCTGGCTTTAAAGAAGCCAATCATTTGATAGGGTGCTACAGTAGTCCGCCTTGAATCTTTCTTTGTATGTGTTTGGGGCCTGAAACCATCATTATTTTCATTATATATATGTATATATGTTTTTCATGACTACCATAATTTGCATGTGTTTGTGTTTTTGGAAATTGATTCAAGAAACATGTCCTGCGGTTAGTGGCT
CCACTGCATAATACAGAG

>TRINITY_DN112379_c3_g1_i8 from sample A3 / Example unspliced isoform:

CCGAACCTCCCCGCTTACAACACAAATTCGCACGCAAAATCCCACGTCACGGATTTACTTGTGAAGTGAAAATGTGGCTTATTGTTGTTAAGAACAGAAGTGACCTTCCTCAATTAGGAAGACCATTGTTGCAACAGAAAGTATTGTGGGTTCGGGGATCGAATCTCTCAGGGGTCTCGTTTCAGGAATATGAGCATGGT
TAGAAATGGTGGGTCATGGTAATCGAAATACTAGTTTTACACGTGGAGCCATTCATCATTCTGGAAGGATTGGGATGCGCTTAGTAGATATTGAAGGTCAAGAAGCTTCACCAAGCACAAGGAACAGAGCTGCAAACACAAGACAAAGGGAGACAATGGGCAACTCTCAAAATCATCATTTGGACACAGATGATAACAGT
GACAGAAATGATGAAGGTCAAAATACAGATGATGCTCTTGAAGAATCCGCCAGACTTTACCATGAACTTGCACATATAACAAAACTGGGGTCAGCACCAAATGTTATGCTATCCAGAATAAATGGTAAGAGCCAGAATGGCTATGTTTCAACATTATCTATGTTGGCAGGAAGAGAAGCCAATATTTCTGGGAGAGGGAA
ATTTAGTGTAGCTGATTGCCGTCATGTCGCAAGTAGATATTTGCCAATTTGTGGCCCAGCTATAATGGAGAGGATGAGGAGTAGAGCATATGTTGGACAATTTTCAGCAGATGGCTCTCTTTTTGTCACAGGATTTCAGGATCATCGGATAAGAATATACAATGTGGAAAATAGATGGAGAGTTCAAAAGGAAGTGCGTG
CTAGAAACTTGCGTTGGACAATCACTGACACTTCTCTTTCACCTGATCAGCGTTACCTTGTGTATGCTACTATCTCTCCTATTGTCCATATCGTGAATGTTGGAAGCAGTATCACAGAATCCTTAGCAAACGTCACAgtatgtctcttctctccttgcccatatcatgcatggttgtaaagagtgaatggaacatgtgaa
catggttcaaacctgttagattttatgttcaagtgagggaatagggatgaggaggggggtgagagggagatggatttcatgcctacaccaaatatatgcatacttaattgtgatttgaagcactgacatcatagatacattcatagtgcaaatagaaccttatggagatgtctgattaagtcaatctttgcagGAAATTC
ACGAGGGGTTAGACTTCTCTAAGTATGAAGAAGAATGTTCATTTGGACTTTTCTGTGTTAAGTTTTCAACCGATGGACGAGAACTTGTTGCTGGCGGCAATGACAAATCTATATATGTTTATGATCTTGAAGCGAGCAAGCGTTCTTTAAAGATAGTGGCTCATGAGGTGTTGTAACTTGGTGATACATCCACAAGCATA
GCAGTTTTTAAGTTTGTCAAAGCTATTCTTGTGCAAGATATCCATAATGTCTTTGTGATCTCTTTAGATTCATATCTCAATGGATTAATTTTCCTGCCA