sp|Q9LDR4|ERG24_ARATH ----> Delta(14)-sterol reductase

Intron 1 GTATATACCT ... TCTCTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	13.89	31	5	2
A2	8	23	2	2
A3	22.86	54	16	4
A4	14.29	24	4	0
A5	8.7	21	2	1
A6	11.59	145	19	0
A7	10.48	111	13	0
A8	6.08	108	7	0
A9	9.76	111	12	0
A10	22.61	89	26	0

>TRINITY_DN119050_c10_g1_i1 from sample A3 / Example spliced isoform

GCGGGTGTTCGTGTTCAAAGAAGTTCTGTTGCGTTTTGCTCGCAGTGAAGAAAAATGAACGCAGGCTCTCTTATGGCAGCTCTAAGGGACTGCCCATTACTGGGATTTCCCTCATGGACCATGGCCGGAATTATTTTGGCATATTTTATTTATCTGGCTTTAGCTGGATTTCTTCTTCCCGGGAAGCTGATTCCTGGCGT
TGTGCTTGCTGATAAGACGAGGATTCACTATCGCTGCAATGGTTTTATCACTCTTCTTCTGCTGGTTACTCTTTTAGGAATCAGTATGGCAGCAGGGCTCTTGTCACCAGCGGTGGTGGCAGGTAAAGGTGGGGAGCTACTTTCTACAACGCTGATATTAAGTGTTTTGGTGGTTTGGGATACAATTAAATCCACAATTT
ATGGGCATTGACCTCAAATTCTTTTTCGTTCGTGCTGGGATGATTGGTTGGGTCGTCATCAATCTCTCAGTTGCAGCAAAAGCCTTGCAACAGAAGGATCCATTTAGCCTTTCAATGTCTCTTTATCAAATTTTTTGTTTGTTATATGTGATGGATTACTTCTGGTATGAAGAGTATATGACGTCCACTTGGGACATAAT
TGCGGAGAATCTTGGTTTCATGTTGGTCTTTGGGGACTTGGTTTGGATTCCATTCACTTTCAGTATCCAGGGTTGGTGGCTTTTAACACACAAACCTGACCTTACAAAAGCTGCTGCCATTCTTAATGTTCTAATCTTTATAATTGGATATCGGGTTTTCAGAGGCTCAAATAAACAGAAGCATATCTTCAAAAAAGATC
CAACGGCTCATATATGGGGTGAGTCTCCAAAGGTTATTGGGGGAAAGTTGCTAGCTTCAGGTTATTGGGGCATATCCAGACACTGTAATTATCTCGGTGACTTACTTCTAGCCTTCTCTTTTAGTTTGCCTTGTGGAGCAAGCTCTTTCGTTCCTTACTTTTACCCTATGTATCTACTAATCCTACTACTTTGGAGAGAG
CGAAGAGATGAGACAAAGTGTCGCGAAAAATACAAGGAAGATTGGGTTACATACTGCAAAGTTGTACCATGGAGAATAATACCATACTTGTATTAGTTGTCATTGAATTTGAATTTTTTGTTATTCAATGCATGTTTTCTCCTTACAGGAATTGCCAGCTCTGAGTGTTTTGAGAAAATCTTCATCTTTGTGGGCATTGT
TCTGTAGACTCTGGGTTTCAAACCTGGATGGTTCACAGTGGTGATTGAAATATGTATGAAAATGTTGTCTTTTGTCTATATTGGGTCTTTGATCTCCCGTGACTTAAAACGATTTTGGACTTTGGTGATACAGAAAAGCTAATAATAACATCAATTTTGAAGATAATTTAGCCTGATTACTGACGTTCATGTTTAATGTA
TGAAATCAAGACAATTACTAGACAAGAATGGAGC

>TRINITY_DN119050_c10_g1_i8 from sample A3 / Example unspliced isoform:

GCGGGTGTTCGTGTTCAAAGAAGTTCTGTTGCGTTTTGCTCGCAGTGAAGAAAAATGAACGCAGGCTCTCTTATGGCAGCTCTAAGGGACTGCCCATTACTGGGATTTCCCTCATGGACCATGGCCGGAATTATTTTGGCATATTTTATTTATCTGGCTTTAGCTGGATTTCTTCTTCCCGGGAAGCTGATTCCTGGCGT
TGTGCTTGCTGATAAGACGAGGATTCACTATCGCTGCAATGGTTTTATCACTCTTCTTCTGCTGGTTACTCTTTTAGGAATCAGTATGGCAGCAGGGCTCTTGTCACCAGCGGTGGTGGCAGGTAAAGGTGGGGAGCTACTTTCTACAACGCTGATATTAAGTGTTTTGATTCTTTTTCGTTCGTGCTGGGATGATTGGT
TGGGTCGTCATCAATCTCTCAGTTGCAGCAAAAGCCTTGCAACAGAAGGATCCATTTAGCCTTTCAATGTCTCTTTATCAAATTTTTTGTTTGgtatatacctttatcaggctgcaattttattgatcatctttgcatgttctttgtcattttaatgagcaattaactgtgttagtgcatctatgacagtgtgaaaatga
cttgttccatccatttgaccaactgatttctcttacagTTATATGTGATGGATTACTTCTGGTATGAAGAGTATATGACGTCCACGTAAGTAAATTGCCTTTCTGTTTCTGCTAAATATCGA

Intron 2 GCCGGAATTA ... CTGGCTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.2	13	50	49
A2	84.38	5	29	25
A3	70.97	9	22	22
A4	84.13	5	23	30
A5	65.38	9	16	18
A6	85.11	14	74	86
A7	79.44	22	79	91
A8	87.59	9	63	64
A9	85.11	14	78	82
A10	83.64	9	43	49

>TRINITY_DN110069_c3_g1_i4 from sample A5 / Example spliced isoform

GTGCAATGCAGTAGGAGCCACTTCACTACTAAAACCTCCCGAGCGGGTGTTCGTGTTCAAAGAAGTTCTGTTGCGTTTTGCTCGCAGTGAAGAAAAATGAACGCAGGCTCTCTTATGGCAGCTCTAAGGGACTGCCCATTACTGGGATTTCCCTCATGGACCATGCTGGATTTCTTCTTCCCGGGAAGCTGATTCCTGGC
GTTGTGCTTGCTGATAAGACGAGGATTCACTATCGCTGCAATGGTTTTATCACTCTTCTTCTGCTGGTTACTCTTTTAGGAATCAGTATGGCAGCAGGGCTCTTGTCACCAGCGCCGAGTTGGTGAGGCCACTTCTTTGTCAGCCAAAATCTGTTTGTGAGAGGACCGAACCTTTGTTGAGGGTCTCTTACCTTAGC

>TRINITY_DN110069_c3_g1_i1 from sample A5 / Example unspliced isoform:

GTGCAATGCAGTAGGAGCCACTTCACTACTAAAACCTCCCGAGCGGGTGTTCGTGTTCAAAGAAGTTCTGTTGCGTTTTGCTCGCAGTGAAGAAAAATGAACGCAGGCTCTCTTATGGCAGCTCTAAGGGACTGCCCATTACTGGGATTTCCCTCATGGACCATGgccggaattattttggcatattttatttatctggc
tttagCTGGATTTCTTCTTCCCGGGAAGCTGATTCCTGGCGTTGTGCTTGCTGATAAGACGAGGATTCACTATCGCTGCAATGGTTTTATCACTCTTCTTCTGCTGGTTACTCTTTTAGGAATCAGTATGGCAGCAGGGCTCTTGTCACCAGCGGTGGTGGCAGGTAAAGGTGGGGAGCTACTTTCTACAACGCTGATAT
TAAGTGTTTTGATTTCATTGTTCTTATATGTTGCTGGTTACCTATCCCAAAGCAAAACAACTTCTTTAAAACCACATGTTACGGGGAACTTTATTCATGACTGGTGGTTTGGGATACAATTAAATCCACAATTTATGGGCATTGACCTCAAATTCTTTTTCGTTCGTGCTGGGATGATTGGTTGGGTCGTCATCAATCTC
TCAGTTGCAGCAAAAGCCTTGCAACAGAAGGATCCATTTAGCCTTTCAATGTCTCTTTATCAAATTTTTTGTTTGTTATATGTGATGGATTACTTCTGGTATGAAGAGTATATGACGTCCACTTGGGACATAATTGCGGAGAATCTTGGTTTCATGTTGGTCTTTGGGGACTTGGTTTGGATTCCATTCACTTTCAGTAT
CCAGGGTTGGTGGCTTTTAACACACAAACCTGACCTTACAAAAGCTGCTGCCATTCTTAATGTTCTAATCTTTATAATTGGGTATGGAAGTCTACTTCCAAGTGCTTTGATTATTCCATCACAATGCAAAGCTGAAGAGTGTAGTCTAGAAACTTGTG

Intron 3 GTATGGATTT ... ATCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.88	25	0.7	7
A2	9.68	28	1	3
A3	16.13	26	4	5
A4	17.24	24	2.7	5
A5	11.76	15	0.9	2
A6	20.75	168	7.9	44
A7	20.3	137.4	5.6	35
A8	18.01	132	5.4	29
A9	14.54	135.2	5.3	23
A10	18.71	113	8.1	26

>TRINITY_DN119050_c10_g1_i2 from sample A3 / Example spliced isoform

GTTAAGTTTCTGGTCAAAAGCTCTTTTGAAATGTAGATGTGCTTTTATTTTGAACCATTGGGACAAGTGTATCTCTCTCAAGCTGTAACTCAATCTGCAGATATCGGGTTTTCAGAGGCTCAAATAAACAGAAGCATATCTTCAAAAAAGATCCAACGGCTCATATATGGGGTGAGTCTCCAAAGGTTATTGGGGGAAAG
TTGCTAGCTTCAGGTTATTGGGGCATATCCAGACACTGTAATTATCTCGGTGACTTACTTCTAGCCTTCTCTTTTAGTTTGCCTTGTGGAGCAAGCTCTTTCGTTCCTTACTTTTACCCTATGTATCTACTAATCCTACTACTTTGGAGAGAGCGAAGAGATGAGACAAAGTGTCGCGAAAAATACAAGGAAGATTGGGT
TACATACTGCAAAGTTGTACCATGGAGAATAATACCATACTTGTATTAGTTGTCATTGAATTTGAATTTTTTGTTATTCAATGCATGTTTTCTCCTTACAGGAATTGCCAGCTCTGAGTGTTTTGAGAAAATCTTCATCTTTGTGGGCATTGTTCTGTAGACTCTGGGTTTCAAACCTGGATGGTTCACAGTGGTGATTG
AAATATGTATGAAAATGTTGTCTTTTGTCTATATTGGGTCTTTGATCTCCCGTGACTTAAAACGATTTTGGACTTTGGTGATACAGAAAAGCTAATAATAACATCAATTTTGAAGATAATTTAGCCTGATTACTGACGTTCATGTTTAATGTATGAAATCAAGACAATTACTAGACAAGAATGGAGC

>TRINITY_DN119050_c10_g1_i5 from sample A3 / Example unspliced isoform:

GGATTCTTACCAGGAAGTACCAATTTTACATAGAAATAAGAAATGTTCTCAGTAGTAAGATTATTTTTTTTCTTGCAGGGGCATATCCAGACACTGTAATTATCTCGGTGACTTACTTCTAGCCTTCTCTTTTAGTTTGCCTTGTGGAGCAAGgtatggatttctggcaccacatttttactgctgctgtatctacttga
ttgtcaccaagtgtgccttcacctttataggtatattgattttattcttataaatctggtgggcatctttgcagCTCTTTCGTTCCTTACTTTTACCCTATGTATCTACTAATCCTACTACTTTGGAGAGAGCGAAGAGATGAGACAAAGTGTCGCGAAAAATACAAGGAAGATTGGGTTACATACTGCAAAGTTGTACC
ATGGAGAATAATACCATACTTGTATTAGTTGTCATTGAATTTGAATTTTTTGTTATTCAATGCATGTTTTCTCCTTACAGGAATTGCCAGCTCTGAGTGTTTTGAGAAAATCTTCATCTTTGTGGGCATTGTTCTGTAGACTCTGGGTTTCAAACCTGGATGGTTCACAGTGGTGATTGAAATATGTATGAAAATGTTGT
CTTTTGTCTATATTGGGTCTTTGATCTCCCGTGACTTAAAACGATTTTGGACTTTGGTGATACAGAAAAGCTAATAATAACATCAATTTTGAAGATAATTTAGCCTGATTACTGACGTTCATGTTTAATGTATGAAATCAAGACAATTACTAGACAAGAATGGAGC