NA ----> NA

Intron 1 GTAATGCTGT ... TTAGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.64	89	9	24
A2	19.56	109	20	33
A3	21.17	108	35	23
A4	18.44	115	25	27
A5	26.47	50	14	22
A6	20.2	571	117	172
A7	20.89	515	126	146
A8	20.25	378	82	110
A9	20.36	616	139	176
A10	19.83	552	126	147

>TRINITY_DN48541_c0_g2_i2 from sample A10 / Example spliced isoform

GATTTCCAATTTTTTTTACCGGTGGCGGTCTTCAAGGGACTGACTTCTCTGAGCACCATGCTGATTATTAATGCTCAGAGTTACACCTTTAAACCCTAAAGTTGAGAACAGCCAGATTTCCACCCCATTTTCTTGGTCGATCCGAGTCATTCACCCCAACTCCAACTGATAAATCTTTTTAAACCCATCTCTAAGCCTTG
TTCTTTTCCAAAATACAACGCCCAAAGAAAGAACTCGGAGTTTCATGTTCGTAATTGCTTGAAATACCGCACCGGATCATCAGGACAAAGCTTCTCTCTTCTGCATGTTCCTCAAGAAAACAAATACAGATAAATCGGACCACTGAGCCGCCCAGTTCGTATCGTAGCGCAGGTTCAATTGTCCGCGTAGGTTCAATTCT
CCAGAAGGGATCTCAGACAGCGAATGGAATACCCACTACCTGCTCTATATGGCGGCCACTATGCTTCCTACTATGGCAGCGGAGGCAGGGCCAACTATTACGCGCTGTGGGCACGCTCAGCTGGAAGCACCGGCAGGACGGACAACGACTTCCCCGGCGTGCGCCTCCGTGGGCTCCCGTACCTGTGCACAGAGAGCGAC
GTATGCGACTTCTTCTCAGAGCTGGACATCGTGGACATTTTGTTCGTAAAGAAGCAGGGCTTGTTCTTGGGCGAGGCCTTGGTTCTTTTTGGCCTGGCCTGGAAGGTGAACATCGCGATACGGAAAAACGGGCATACCATAAAGAATCAGCACATCCAGGTCTTCCGTTGCAGGAAGACGGAATATTACCATACCATCTT
TGCCCAAATCAATCACGAGATTGAGCTCACCCATCGCAGACACGGCCATAACGAGGTTGATGGTGTTCTCAGGATGGACGGCCTCCCATTTTCTGCCACAAAGAAAGATGTCATTGATTTCTTCACTGGTTTTGCGGTGTCTGACGAGAGCGTGCATCTTGTATTTGAGTCTGATGAGAAGACCTGGGGACACCATGCGT
ATGTTCAGTTTGAGAGTGCAGAGCAGTCCAAGGCCGCCATGAGAAGAGACAGGATGATGGTCGGCCGTAGGTATGTCAAATTGTTTCCCTCCACGCATCAAGAAATGGCCACTGCAGTCTCCAACTCCCGCCATTAAATGCCATGAAATAACCTAATGACAACAGACTGCTTTAATTTTTTGAAGAGGTTTCTGCATCTA
CTTCATTTTCTGGACTATAATTTCGTTATATCTTGGACCCTGCATGGCCATCTTCACTGGCTTGAATTTCATTCTTGAAGCTATATCTGTATTTCTTGGTCCGGTTCACCCGATCTCTTGATTTTTTTTAAATAAACTTTCAATTAATAAAAAATGGAAA

>TRINITY_DN48541_c0_g2_i4 from sample A10 / Example unspliced isoform:

TTTCCAATTTTTTAGCCGTGGCGGTCTTCGAGGGACTGATTTCTCTGAGCACCATGCTGATTATTAATGCTCAGAGTTACACCTTTAAACCCTAAAGTTGAGAACAGCCAGATTTCCACCCCATTTTCTTGGTCGATCCGAGTCATTCACCCCAACTCCAATGATAAATCTTTTTAAACCCATCTCTAAGCCTTGTTCTT
TTCCAAAATACAACGCCCAAAGAAAGAACTCGGAGTTTCATGTTCGTAATTGCTTGAAATACCGCACCGGATCATCAGGACAAAGCTTCTCTCTTCTGCATGTTCCTCAAGAAAACAAATACAGATAAATCGGACCACTGAGCCGCCCAGTTCGTATCGTAGCGCAGGTTCAATTGTCCGCGTAGGTTCAATTCTCCAGA
AGgtaatgctgtcatatcatcaagcaacgtctctagtatttttgtttgaatctctgcgccattagatttcattttgttgtaaatttagtttcagGGATCTCAGACAGCGAATGGAATACCCACTACCTGCTCTATATGGCGGCCACTATGCTTCCTACTATGGCAGCGGAGGCAGGGCCAACTATTACGCGCTGTGGGCA
CGCTCAGCTGGAAGCACCGGCAGGACGGACAACGACTTCCCCGGCGTGCGCCTCCGTGGGCTCCCGTACCTGTGCACAGAGAGCGACGTATGCGACTTCTTCTCAGAGCTGGACATCGTGGACATTTTGTTCGTAAAGAAGCAGGGCTTGTTCTTGGGCGAGGCCTTGGTTCTTTTTGGCCTGGCCTGGAAGGTGAACAT
CGCGATACGGAAAAACGGGCATACCATAAAGAATCAGCACATCCAGGTCTTCCGTTGCAGGAAGACGGAATATTACCATACCATCTTTGCCCAAATCAATCACGAGATTGAGCTCACCCATCGCAGACACGGCCATAACGAGGTTGATGGTGTTCTCAGGATGGACGGCCTCCCATTTTCTGCCACAAAGAAAGATGTCA
TTGATTTCTTCACTGGTTTTGCGGTGTCTGACGAGAGCGTGCATCTTGTATTTGAGTCTGATGAGAAGACCTGGGGACACCATGCGTATGTTCAGTTTGAGAGTGCAGAGCAGTCCAAGGCCGCCATGAGAAGAGACAGGATGATGGTCGGCCGTAGGTATGTCAAATTGTTTCCCTCCACGCATCAAGAAATGGCCACT
GCAGTCTCCAACTCCCGCCATTAAATGCCATGAAATAACCTAATGACAACAGACTGCTTTAATTTTTTGAAGAGGTTTCTGCATCTACTTCATTTTCTGGACTATAATTTCGTTATATCTTGGACCCTGCATGGCCATCTTCACTGGCTTGAATTTCATTCTTGAAGCTATATCTGTATTTCTTGGTCCGGTTCACCCGA
TCTCTTGATTTTTTTTAAATAAACTTTCAATTAATAAAAAATGGAAA

Intron 2 GTTTCTTCCT ... TTAACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.69	348	1212	231
A2	77.72	321	1120	187
A3	80.73	270	1131	157
A4	78.53	219	801	131
A5	79.37	157	604	88
A6	85.14	2056	11779	86
A7	83.31	1982	9892	153
A8	84.98	2161	12227	102
A9	85.2	1686	9705	128
A10	83.65	1393	7129	167

>TRINITY_DN45991_c0_g1_i11 from sample A8 / Example spliced isoform

AGTGGGAGCTTCAACCTGTTAAATTAATCGGAGCCTCTTTCCAGGTCCAGGAAAAGGAGGTTCCGGCTCCACACCAGAATCAGACGGAACTGAATTCAAGGCTAATGGTGTCGTTTAAAGCATTAAGGCATTCTACAATGATAGAGGCCAGTGAAAGTTCATTCGGACGATGTTGTGATTGCATGGGAGGTACCATACCG
TGGTGGCAACAAATGCTATATTGTGGCGTGTGGCAGTAGTTTAAAGTTCCTCCTTGAACCTTATATGAAGCTCCCATGGCGGACGCTAACATCCCAGACGGCCAAGAAATCGAAAACCCAAAATCCGCAGAACAGATCCCCCAGTCCAGTGTGCAGGAGCAGGACAGATATTCAAATGTTGAAAGATGCATATCAGAACA
CTCAAAACACTAAACCAATACATGAAACATCTCATGTTATGAAAGATGCATCGATTGAAGTTCTACTGCAGTATTATGGGCGCACTACAATTATGGTTTTATTTCATTCAATGTTGCAGGAGCATAAACGCAATTGCATGCTCAAGAACAAAAATGAACTGGGTGCCCAAGAAGAGATCGGTGGTTCCCCAGGGGGCAAC
AATCAAATTGAGTGCCCAACAATCGGATTGAGTGCCGAGGTACCAAATTTGGTTATAGATTAGGTTTAAAATATGATGCAATTGCATGCTCAG

>TRINITY_DN45991_c0_g1_i2 from sample A8 / Example unspliced isoform:

ACGACAATGGGTAATCCTCTATTTTTTTTTGTTTAATCCTAGCCTCTTGCCAGGTGGGAGCTTCCACCTGTTAAATTAATCGGAGCCTCTTGCCAGGTGAGAGCTTCAATGGGTTAATTTGTAGTGGAGGAGGACCGCGGTTAAATGAATGGTCCTCGTTTCAGTTTCAGTTTCAAGGGAATACCTAATATATTAGGTTA
CTCCGTGTCTTTCAATTTCTTTTGAGGTTGTTTGTTTTCTTTTGCAGGTCCAGGAAAAGGAGGTTCCGGCTCCACACCAGAATCAGACGGAACTGAATTCAAGGCTAATGGTGTCGTTTAAAGCATTAAGGCATTCTACAATGATAGAGGCCAGTGAAAGTTCATTCGGACGATGTTGTGATTGCATGGGAGGTACCATA
CCGTGGTGGCAACAAATGCTATATTGTGGCGTGTGGCAGTAGTTTAAAGTTCCTCCTTGAACCTTATATGAAGCTCCCATGGCGGACGCTAACATCCCAGACGGCCAAGAAATCGAAAACCCCAAATCCGGCGAACAGATCCCCCAATCCAGTGTGCAGGAGCAGGACAGgtttcttcctatcgccaacattagctgcat
catgaagataaagtttctgcatcttcttcattttcttgactatagtttcgttatatcttggaccctgcatagccatcttcactggcttgaatttctttggcagactaaaccattcatgaagctaatctctatttcttggtcctgttcactcgatttcttgatttttttaaataaactttcaataataatgatgcgcaaga
ttttatgtggggggacagttgttttcaaaatatttgttaataaaaaatatttcagatttattttactattagatttgtgatttattagataacgattttgaattttttgaaattaactacagATATTCAAATGTTGAAATATTTTATGTTGAAAGATGCATCTCAGAACACTCAAAACACTAAACCAATACATGAAACAT
CTCATGTTATGAAAGATGCATCGATTGAAGTTCTACTGCAGTATTATGGGCGCACTACAATTATGGTTTTATTTCATTCAATGTTGCAGGAGCATAAACGCAATTGCATGCTCAAGAACAAAAATGAACTGGGTGCCCAAGAAGAGATCGGTGGTTCCCCAGGGGGCAACAATCAAATTGAGTGCCCAACAATCGGATTG
AGTGCCGAGGTACCAAATTTGGTTATAGATTAGGTTTAAAATATGATGCAATTGCATGCTCAG

Intron 3 GTAATGCTGT ... GTAAATTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.72	88	7.7	23
A2	21.8	104	16.7	29
A3	15.45	104	29.7	19
A4	18.12	113	21.2	25
A5	25.86	43	12.7	15
A6	20.21	537	98.2	136
A7	18.02	473	105.5	104
A8	20.71	363	68.7	94.8
A9	18.31	566	116.2	126.8
A10	18.11	520	105.5	115

>TRINITY_DN112025_c3_g1_i1 from sample A3 / Example spliced isoform

CGGTCTTCGAGGGACTGATTTCTCTGAGCACCATGCTGATTATTAATGCTCAGAGTTACACCTTTAAACCCTAAAGTTGAGAACAGCCAGATTTCCACCCCATTTTCTTGGTCGATCCGAGTCATTCACCCCAACTCCAACTGATAAATCTTTTTAAACCCATCTCTAAGCCTTGTTCTTTTCCAAAATACAACGCCCAA
AGAAAGAACTCGGAGTTTCATGTTCGTAATTGCTTGAAATACCGCACCGGATCATCAGGACAAAGCTTCTCTCTTCTGCATGTTCCTCAAGAAAACAAATACAGATAAATCGGACCACTGAGCCGCCCAGTTCGTATCGTAGCGCAGGTTCAATTGTCCGCGTAGGTTCAATTCTCCAGAAGTTTCAGGGATCTCAGACA
GCGAATGGAATACCCACTACCTGCTCTATATGGCGGCCACTATGCTTCCTACTATGGCAGCGGAGGCAGGGCCAACTATTACGCGCTGTGGGCACGCTCAGCTGGAAGCACCGGCAGGACGGACAACGACTTCCCCGGCGTGCGCCTCCGTGGGCTCCCGTACCTGTGCACAGAGAGCGACGTATGCGACTTCTTCTCAG
AGCTGGACATCGTGGACATTTTGTTCGTAAAGAAGCAGGGCTTGTTCTTGGGCGAGGCCTTGGTTCTTTTTGGCCTGGCCTGGAAGGTGAACATCGCGATACGGAAAAACGGGCATACCATAAAGAATCAGCACATCCAGGTCTTCCGTTGCAGGAAGACGGAATATTACCATACCATCTTTGCCCAAATCAATCACGAG
ATTGAGCTCACCCATCGCAGACACGGCCATAACGAGGTTGATGGTGTTCTCAGGATGGACGGCCTCCCATTTTCTGCCACAAAGAAAGATGTCATTGATTTCTTCACTGGTTTTGCGGTGTCTGACGAGAGCGTGCATCTTGTATTTGAGTCTGATGAGAAGACCTGGGGACACCATGCGTATGTTCAGTTTGAGAGTGC
AGAGCAGTCCAAGGCCGCCATGAGAAGAGACAGGATGATGGTCGGCCGTAGGTATGTCAAATTGTTTCCCTCCACGCATCAAGAAATGGCCACTGCAGTCTCCAACTCCCGCCATTAAATGCCATGAAATAACCTAATGACAACAGACTGCTTTAATTTTTTGAAGAGGTTTCTGCATCTACTTCATTTTCTGGACTATA
ATTTCGTTATATCTTGGACCCTGCATGGCCATCTTCACTGGCTTGAATTTCATTCTTGAAGCTATATCTGTATTTCTTGGTCCGGTTCACCCGATCTCTTGATTTTTTTTAAATAAACTTTCAATAATAATGATGCGCAAGATTTTATGTGGAA

>TRINITY_DN112025_c3_g1_i2 from sample A3 / Example unspliced isoform:

CGTAGGTTCAATTCTCCAGAAGgtaatgctgtcatatcatcaagcaacgtctctagtatttttgtttgaatctctgcgccattagatttcattttgttgtaaatttagTTTCAGGGATCTCAGACAGCGAATGGAATACCCACTACCTGCTCTATATGGCGGCCACTATGCTTCCTACTATGGCAGCGGAGGCAGGGCCA
ACTATTACGCGCTGTGGGCACGCTCAGCTGGAAGCACCGGCAGGACGGACAACGACTTCCCCGGCGTGCGCCTCCGTGGGCTCCCGTACCTGTGCACAGAGAGCGACGTATGCGACTTCTTCTCAGAGCTGGACATCGTGGACATTTTGTTCGTAAAGAAGCAGGGCTTGTTCTTGGGCGAGGCCTTGGTTCTTTTTGGC
CTGGCCTGGAAGGTGAACATCGCGATACGGAAAAACGGGCATACCATAAAGAATCAGCACATCCAGGTCTTCCGTTGCAGGAAGACGGAATATTACCATACCATCTTTGCCCAAATCAATCACGAGATTGAGCTCACCCATCGCAGACACGGCCATAACGAGGTTGATGGTGTTCTCAGGATGGACGGCCTCCCATTTTC
TGCCACAAAGAAAGATGTCATTGATTTCTTCACTGGTTTTGCGGTGTCTGACGAGAGCGTGCATCTTGTATTTGAGTCTGATGAGAAGACCTGGGGACACCATGCGTATGTTCAGTTTGAGAGTGCAGAGCAGTCCAAGGCCGCCATGAGAAGAGACAGGATGATGGTCGGCCGTAGGTATGTCAAATTGTTTCCCTCCA
CGCATCAAGAAATGGCCACTGCAGTCTCCAACTCCCGCCATTAAATGCCATGAAATAACCTAATGACAACAGACTGCTTTAATTTTTTGAAGAGGTTTCTGCATCTACTTCATTTTCTGGACTATAATTTCGTTATATCTTGGACCCTGCATGGCCATCTTCACTGGCTTGAATTTCATTCTTGAAGCTATATCTGTATT
TCTTGGTCCGGTTCACCCGATCTCTTGATTTTTTTTAAATAAACTTTCAATAATAATGATGCGCAAGATTTTATGTGGAA