sp|Q9ZUC1|AOR_ARATH ----> NADPH-dependent alkenal/one oxidoreductase, chloroplastic

Intron 1 GTAAGCTTCT ... AGGAGAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	50	3	0	3
A4	0	1	1	0
A5	40	3	2	2
A6	33.33	20	1	10
A7	50	11	1	11
A8	35.29	22	0	12
A9	43.75	18	2	14
A10	48.57	18	2	17

>TRINITY_DN47286_c0_g1_i6 from sample A7 / Example spliced isoform

CGCGGCTACCGCCTTTTCAACGAAATAACTCAACCATAACGGTCTCGACCATCGTGCCCAGCATAGCAGAGCAGAAAGATTCCGAGGAAAAAAAGCATAGCAGAAAGATGAAATTAGAAAAGTGCAAACAAACCTTCGCTCTGAGTTCTGCAATGTCGATCTCACCGAAGTGGTCCTCCGGAATCCAGTCCCCGGTGGCG
GGATCCCTCATCCAGAAAGCCTTATTTTTCCCTTCCGCCTGTTCTTTTCCGCCTGCTGCTTTCGACTGCTGATGCGACGCCACCTCCGGTTTCACTCCATCGCTGCTACCGCCGACCAACGAATTCGCTGCTCCGCGCACTAACGTTGCGTATTGGTTTTTACTTTGAACGTATGCTTTATGCTTTACTAAAGTTGGCAC
CTTTGCTGATATATTCAGATTTTATCATTCACCCCTTTCTGTGGTGAGGTGAGACTTTTTAAGTCATATTGACCCCCTGATCGTCCAAAAAAAGAAGTGCCATTTGAGTGTGAAAGAGGTAAAGGATCCCAGGAAAGGAATGCCGTATGCGGCCCTTATTTGGATGGGAAACGGATTTTTATTCCAAAATATAAACCTTA
TCTCTTAAAACCAAAGTGTTTTCCTAGTTGATGGAGAATATATTGGAAGGTATAATACCATGTGTGGGTAGGCCTTTCGTGATTATTTGAGAAAGGACTATAATGGAATGCAATCTTCTCTTTTTCAGAATTTTTAAAATACAAAATAAGATATATTTTATGTGTAAATATATAAAATATGGAGGGTTTCATTATAGATA
TAAG

>TRINITY_DN47286_c0_g1_i5 from sample A7 / Example unspliced isoform:

CGCGGCTACCGCCTTTTCAACGAAATAACTCAACCATAACGGTCTCGACCATCGTGCCCAGCATAGCAGAGCAGAAAGATTCCGAGGAAAAAAAGCATAGCAGAAAGATGAAATTAGAAAAGTGCAAACAAACgtaagcttcttgattttgatttttttttcatttccataaacaaaaagatttgcaattacagtgcaga
tccattattctattacattttccatgaaatattgtagttatcttctactttctggaggagagcagCTTCGCTCTGAGTTCTGCAATGTCGATCTCACCGAAGTGGTCCTCCGGAATCCAGTCCCCGGTGGCGGGATCCCTCATCCAGAAAGCCTTATTTTTCCCTTCCGCCTGTTCTTTTCCGCCTGCTGCTTTCGACTG
CTGATGCGACGCCACCTCCGGTTTCACTCCATCGCTGCTACCGCCGACCAACGAATTCGCTGCTCCGCGCACTAACGTTGCGTATTGGTTTTTACTTTGAACGTATGCTTTATGCTTTACTAAAGTTGGCACCTTTGCTGATATATTCAGATTTTATCATTCACCCCTTTCTGTGGTGAGGTGAGACTTTTTAAGTCATA
TTGACCCCCTGATCGTCCAAAAAAAGAAGTGCCATTTGAGTGTGAAAGAGGTAAAGGATCCCAGGAAAGGAATGCCGTATGCGGCCCTTATTTGGATGGGAAACGGATTTTTATTCCAAAATATAAACCTTATCTCTTAAAACCAAAGTGTTTTCCTAGTTGATGGAGAATATATTGGAAGGTATAATACCATGTGTGGG
TAGGCCTTTCGTGATTATTTGAGAAAGGACTATAATGGAATGCAATCTTCTCTTTTTCAGAATTTTTAAAATACAAAATAAGATATATTTTATGTGTAAATATATAAAATATGGAGGGTTTCATTATAGATATAAG

Intron 2 GTTAGTCAGA ... GCATGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45	11	12	6
A2	41.38	17	10	14
A3	52	6	2	11
A4	35.14	12	9	4
A5	60	2	4	2
A6	46.67	8	5	9
A7	32.2	20	11	8
A8	11.11	12	1	2
A9	13.51	16	4	1
A10	28	18	11	3

>TRINITY_DN107866_c0_g2_i12 from sample A3 / Example spliced isoform

CCTCATGTCCCTTAGTTAAGGGCTTACTTCCAATGTGGTCGGCGATCTGCCTCATAAGTTTTTCAAGAACGACGACGTGATATTGACCCTCCATTGCGAAATTTGCACAAAACTATTTTGTACTTTTGAAAAAATGCCTCGAGCTCCAGGGGAATGGAGCGGAGAGAGGGCTAAGTTCTGATTTACAGAATATATAAACG
AAAGCAAGCAGATGGACTGATCAAGAACAGGACATTGATTTCTCGAATAATTCTCATGGATTTTATCTGATTTGGGAAGAGGAGAACCAGAGAGTGAGAAAGAGAGAGAGATAGGGAGAGAGAGAGGGAGAGAAAGGAATGAGAGGAGAGACAGGCTCAAACACAACTCGGACACTTATATTATTGCAACATTTTCCACA
ATGACTGGCAATAGAAAGCTTGTGGTTTTAGGCATTCCCTGGGATGTGGATACTGAAGGCTTGAGACAATACATGTGCCAATTTGGTGACTTGGAGGATGTAATTGTAATGAAGGATCGTTTATCGGGACGATCTCGTGGATTTGGATATGTTACCTTCTCCTCTTATGAAGGTGCAGAGAAAGCAGTGGCGAGTCAACA
TGTTTTACAAGGGAGGATTCTTGAGGTCAAAATAGCAACCCCAAAGGATGAGATGAGGCCCACAAGTAGGAAAATCACTCGCATATTTGTTGCGCGAATCTCTCCAACTGTTACAGATGAAATATTTCGAAGCTACTTCGAGAAATATGGCTCCATTCTAGATGCTTATTTGCCAAAGGTGAAATATTGATGCTTTTATT
ATTACTTACC

>TRINITY_DN107866_c0_g2_i8 from sample A3 / Example unspliced isoform:

CCTCATGTCCCTTAGTTAAGGGCTTACTTCCAATGTGGTCGGCGATCTGCCTCATAAGTTTTTCAAGAACGACGACGTGATATTGACCCTCCATTGCGAAATTTGCACAAAACTATTTTGTACTTTTGAAAAAATGCCTCGAGCTCCAGGGGAATGGAGCGGAGAGAGGGCTAAGTTCTGATTTACAGAATATATAAACG
AAAGCAAGCAGATGGACTGATCAAGAACAGGACATTGATTTCTCGAATAATTCTCATGGATTTTATCTGATTTGgttagtcagattatagataatacatatgaatgtctcttcacatctgcatctcatgctaatactctgcctctgctacagaccgtagtgggcaatttctttgtgcggcaaacctcaaccaaggaggat
ttttagtccgttagattgcttaagcctcccaagttgtgtaattttttccccccgtattttttttggttgttttgaaggttgtaaattatgaatcccttgcattgccaagatactagacatgtggggggcaagtccctgggtgatgtgattacatcctcctcctcaggcctcacgcctgtactaatatctctgatgtaatg
tcatgctttgtgagccatctttagcttctgattattttgcgattattatctattagatgtttgaaagtttatccatatgttggaatttcattccacacgaatacttgatttgatgtttaaaagtctatccctatgttggaatttcattccacatgaatacttcatgtgtttctgggcagagtttggtatttttgacatag
gagtaaatatgacaaccataggagtccgagttcagcagcttcttagtcacccatctggctgttttgggtttttaagctacccagttggctgttctggtacttccaagcttctacttgtgcaagctgtaggtgctcttttgaatagtgaaaagaagtcattcttctgactagttgttatatactggtgtggaaatacattg
tattccgtgcccttgagcttagccttgatctgttaaatgaaaggttagcctcagcaaggagtgaatttagtatgtgcatgtgcagGGAAGAGGAGAACCAGAGAGTGAGAAAGAGAGAGAGATAGGGAGAGAGAGAGGGAGAGAAAGGAATGAGAGGAGAGACAGGCTCAAACACAACTCGGACACTTATATTATTGCAA
CATTTTCCACAATGACTGGCAATAGAAAGCTTGTGGTTTTAGGCATTCCCTGGGATGTGGATACTGAAGGCTTGAGACAATACATGTGCCAATTTGGTGACTTGGAGGATGTAATTGTAATGAAGGATCGTTTATCGGGACGATCTCGTGGATTTGGATATGTTACCTTCTCCTCTTATGAAGGTGCAGAGAAAGCAGTG
GCGAGTCAACATGTTTTACAAGGGAGGATTCTTGAGGTCAAAATAGCAACCCCAAAGGATGAGATGAGGCCCACAAGTAGGAAAATCACTCGCATATTTGTTGCGCGAATCTCTCCAACTGTTACAGATGAAATATTTCGAAGCTACTTCGAGAAATATGGCTCCATTCTAGATGCTTATTTGCCAAAGGACCAAACAAC
AAAGCAACATCGTGGAATTGGATTTGTTACATATGAAAATGCAGAGTCTGTTGACAAAGTTATGTCAGAGAGTCATGAGCTCGCGGGATCAACTATTGCTGTAGATCGAGCAAATCCAAAGTGTGATACCCTGAAGGAGGAGACTGGAAAAGTTTGGGAAAGGACCTTTCCTGGAGCCAGTGGTGTTTTTAACAGTCATG
TAAACACAACAGACTTTGGACCTTTTTATGTGTCAGCTTTTGGAGGTTGTGACTTCCCAGGATCAGATAGAGCGGCTATAGGAAATACATATGAAAGCAACTGTGGAAGAACCAACCCATGGTCAGCAGGTCTACCTGCACCACAAGTTGGAGGACCTTTGCCAGGAATACCGGGTATTCATCCATCAGCCATGAATTAT
GGCTCAACTGATCCTAATTTTGCTCCGTCCAGAGTGGGGAGAAAAATATTTGTAGGGCGTATTCCAGTTGAAGCAACAGCTGAAGACTTGCAATTATATTTCAGTCAATTTGGTCAGATTTTGGATGTGTATCTTCCAAAGGATGCAAAGAAAATATCCCATAGAGGTTTTGGTTTTGTGACTTTTGCGGAAGAGTCTAC
TGCTGAGCATGTTTCTCAGAGGAGACATGAGATCCTTGGGCACTTGATTGCAGTTGATCGTGCTTCACCATTGGATGAAACGCCAAGTGTAGGAGTCTATGCTCCTTCTGGAATGGGAAAAAAGATTTTCATTGGTCGCATTCCAGTTGAAGCAACGACTGAAGACTTGAGACTGTATTTTAGCCAGTATGGGTGCATTT
TAGATGTGTACCTTCCAAAGGATGCAAAGAAAATCTCCCACAGAGGATTTGGCTTTGTCACCTTTGCAGAAGAGTCTACAGCTGAGCATGTTGCTCAAAAGACACATGACATTCTTGGGCATCGGATTGCAGTTGATCGTGCAGCACCTTTGGACGAGACACGGAGTGGATTAAAGCCATCGAATGCTTTATTTGGAGGT
GCTGGTGGTCCACTAAGAGGATCTAGCTCTACCATCTCTGGAAGTGCTTATGGAAACACACCTGCTAACCTTGGTTTTAATAGTAGTTGGACTCCATATGGTGGGTTGGGACAAATGGCTGCTTCTGATGGAGGTCCAAGTGGTCATCGGACTTCCAGGATGGAACCCCGTTACAGGCCGTACTAGGATAGAATTGTTGT
GTAAAGTGATGTTAAATTGCCATCATTTTCTTGGGGCTTTTGGAAGATGCTTTTGAAGTTAGATCATTGACATACCTGTCATCATTAAAGAAATTATTTCTGATGCTTTGGTTTATTAATGATGTGCTTTGATTGGAGTGTTAATAACTGTTAGATAGGAAGTTTGGGCTCATATAATTTGCTTGTTTTGCCTATATTTT
GATTTTTTCTATTCATATGGCAATTGACTCAATAATCTGGACTCATCATTCAGACGGTAGCAAGTTTGCTAACCATTGCCACTAATTGATGGTGAAGTTAGTTATTCTAACT

Intron 3 GTAAGCTTCT ... AATATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	-1	0	0	0
A4	50	1	1	1
A5	50	2	2	2
A6	24	19	1	6
A7	38.46	16	1	10
A8	30.43	16	0	7
A9	34.38	21	2	11
A10	43.24	21	2	16

>TRINITY_DN47286_c0_g1_i4 from sample A7 / Example spliced isoform

CGCGGCTACCGCCTTTTCAACGAAATAACTCAACCATAACGGTCTCGACCATCGTGCCCAGCATAGCAGAGCAGAAAGATTCCGAGGAAAAAAAGCATAGCAGAAAGATGAAATTAGAAAAGTGCAAACAAACTTATCTTCTACTTTCTGGAGGAGAGCAGCTTCGCTCTGAGTTCTGCAATGTCGATCTCACCGAAGTG
GTCCTCCGGAATCCAGTCCCCGGTGGCGGGATCCCTCATCCAGAAAGCCTTATTTTTCCCTTCCGCCTGTTCTTTTCCGCCTGCTGCTTTCGACTGCTGATGCGACGCCACCTCCGGTTTCACTCCATCGCTGCTACCGCCGACCAACGAATTCGCTGCTCCGCGCACTAACGTTGCGTATTGGTTTTTACTTTGAACGT
ATGCTTTATGCTTTACTAAAGTTGGCACCTTTGCTGATATATTCAGATTTTATCATTCACCCCTTTCTGTGGTGAGGTGAGACTTTTTAAGTCATATTGACCCCCTGATCGTCCAAAAAAAGAAGTGCCATTTGAGTGTGAAAGAGGTAAAGGATCCCAGGAAAGGAATGCCGTATGCGGCCCTTATTTGGATGGGAAAC
GGATTTTTATTCCAAAATATAAACCTTATCTCTTAAAACCAAAGTGTTTTCCTAGTTGATGGAGAATATATTGGAAGGTATAATACCATGTGTGGGTAGGCCTTTCGTGATTATTTGAGAAAGGACTATAATGGAATGCAATCTTCTCTTTTTCAGAATTTTTAAAATACAAAATAAGATATATTTTATGTGTAAATATA
TAAAATATGGAGGGTTTCATTATAGATATAAG

>TRINITY_DN47286_c0_g1_i5 from sample A7 / Example unspliced isoform:

CGCGGCTACCGCCTTTTCAACGAAATAACTCAACCATAACGGTCTCGACCATCGTGCCCAGCATAGCAGAGCAGAAAGATTCCGAGGAAAAAAAGCATAGCAGAAAGATGAAATTAGAAAAGTGCAAACAAACgtaagcttcttgattttgatttttttttcatttccataaacaaaaagatttgcaattacagtgcaga
tccattattctattacattttccatgaaatattgtagTTATCTTCTACTTTCTGGAGGAGAGCAGCTTCGCTCTGAGTTCTGCAATGTCGATCTCACCGAAGTGGTCCTCCGGAATCCAGTCCCCGGTGGCGGGATCCCTCATCCAGAAAGCCTTATTTTTCCCTTCCGCCTGTTCTTTTCCGCCTGCTGCTTTCGACTG
CTGATGCGACGCCACCTCCGGTTTCACTCCATCGCTGCTACCGCCGACCAACGAATTCGCTGCTCCGCGCACTAACGTTGCGTATTGGTTTTTACTTTGAACGTATGCTTTATGCTTTACTAAAGTTGGCACCTTTGCTGATATATTCAGATTTTATCATTCACCCCTTTCTGTGGTGAGGTGAGACTTTTTAAGTCATA
TTGACCCCCTGATCGTCCAAAAAAAGAAGTGCCATTTGAGTGTGAAAGAGGTAAAGGATCCCAGGAAAGGAATGCCGTATGCGGCCCTTATTTGGATGGGAAACGGATTTTTATTCCAAAATATAAACCTTATCTCTTAAAACCAAAGTGTTTTCCTAGTTGATGGAGAATATATTGGAAGGTATAATACCATGTGTGGG
TAGGCCTTTCGTGATTATTTGAGAAAGGACTATAATGGAATGCAATCTTCTCTTTTTCAGAATTTTTAAAATACAAAATAAGATATATTTTATGTGTAAATATATAAAATATGGAGGGTTTCATTATAGATATAAG

Intron 4 GTCAATACCA ... TTTGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.16	205	38	53
A2	19.44	145	36	34
A3	20.75	231	58	63
A4	18.73	115	30	23
A5	19.05	85	17	23
A6	19.9	783	223	166
A7	20.11	1007	269	238
A8	21.56	1104	293	314
A9	20.33	1027	303	221
A10	20.24	875	258	186

>TRINITY_DN41931_c0_g3_i3 from sample A8 / Example spliced isoform

TTTAGCCATTATCCAAAAAGTAATTTTGGCCCCGTTCAAACAAGCGTTTGATGATGTCTGGGCTTCCCATTTCATGATCAGATCATGGCTGAGCTCAAAATTAAATCCGTGGAGAGTTTCCAATGAGATCATGGCTGAGCTCAAAATTAAATCCGTGGAGAGTTTCCAATGAGATCACGGCTTAAAATAAAATCGACAAC
TTTCTAATCGAAATCCTCCATCTAATGGCGCCGTCACATAACATAAAACACCGCAAGCTTGGATTCTCGCCTTCTAACATCCGACACTCAGTTTCCATAGCTTGAATTGCAGGGAAGACGAACAAAATGAAGGCAGTGGTGATAACAACTCCAGGAGATGCAGATGTTCTGCAATTACAAGATGTAGATGACCCCCAAAT
CAACGACAATGAAGTGCTGATAAAAATCTCGGCAACGGCCCTGAATAGGGCAGATACCCTACAAAGACAAGGGAGGTACCCTCCGCCGAAAGACGCACCGCCTTATCTCGGTCTCGAATGCTCAGGCATTATTGAAGCCGTCGGCAAAAATGTACAGAACTGGAAAGTTGGCGATGAGGTTTGTGCACTTCTGGGAGGAG
GAGGGTATGCAGAAAAGGTGGCCGTTCCATCAGGGCATGTCTTGCCTGTTCCAAAAGGCATTTCATTACAAGAAGCTGCAGCCCTTCCTGAGGTGGCATGCACTGTGTGGTCTACAGTTTTTATGACAAGTCATCTGTCCTCAGGCGAGAGCATTCTGGTGCATGGAGGCTCTAGTGGTATTGGAACGTTTGCTATTCAG
ATAGCCAAGTACTATGGAGCAAGAGTGTTTGTTACAGCTGGAAACGAGGAAAAACTAGCAAGTTGTAAGGAGCTTGGTGCTGATGTGGCAATAAATTACAAAACAGAAGATTTTGTTGCACGTGTAAAAGAAGAAACAGGAGGAAAAGGTGTTGATGTAATATTAGATAATATGGGCGGCTCTTATCTTCAGAGGAATTT
AGATGCCTTGGGCCTCGATGGGAGATTATTTCTCATTGGCTTTCAAGGGGGTTCTGTTGCACAGATCAACTTATCAACTATATTTGCAAAGCGCCTGACTATCCAAGCTGCGGGCTTGCGGTCTCGCTCTGCAGAGAACAAAGAACAAATCATCTCTGATGTTCACAAGAATGTTTGGCCTGCATTTGAAGAAGGAAAAG
TGAAGCCCATCATTTACACCTCACTTCCCTTGTCACAAGCTGCAGAGGCCCACAGGATTATGGAATCCAGCACACATATTGGCAAAATCCTGCTTGTAGTTTAGCAATTGTATGTGAAGAGGGATGGAATAAAGATATTCTCCCCATCTCAATTCTTTTTTTATATATTCAGAGGATAGCTAAAGTGAAACGTCTTACAC
ACTATCGAATGCCTTTTATTATCTATTGTTTGAAGTTATGGGAAATCCATTAGTCTAAAAATTTCTTGCTGGGAACGCTGGCTATTTGTAATTCTGCTTAAGCTATTTTAGAATGGTTGAAAACTCGTGGACTATTTGAATTAATAGTAGGTGAATGTGTCTTTGCATGAGTGAGAGAGAAAGAATGAGAGATTTTTTTT
ACTAACATCTGTGTCACCAAACAATGTATTGTACTTGTAACAAAGGATTACAGTAAAATAAAAATCATCATTGAGTGGTTAGATTTAAACATGAAACATAGCCAAGGGATTAGTTAAACCAGTTATTGTCAATGTTGCTGGGCATTTGTCTAGTTCTATGCCAGCAAGTGCTCAGTTTGATTTTTTTGGGTCAGGTACAC
TAGTCAATGCTGAC

>TRINITY_DN41931_c0_g3_i1 from sample A8 / Example unspliced isoform:

AGGTTTGTGCACTTCTGGGAGGAGGAGGGTATGCAGAAAAGGTGGCCGTTCCATCAGGGCATGTCTTGCCTGTTCCAAAAGGCATTTCATTACAAGAAGCTGCAGCCCTTCCTGAGGTGGCATGCACTGTGTGGTCTACAGTTTTTATGACAAGTCATCTGTCCTCAGGCGAGAGCATTCTGGTGCATGGAGGCTCTAGT
GGTATTGGAACGTTTGCTATTCAGATAGCCAAGTACTATGGAGCAAGAGTGTTTGTTACAGCTGGAAACGAGGAAAAACTAGCAAGTTGTAAGGAGCTTGGTGCTGATGTGGCAATAAATTACAAAACAGAAGATTTTGTTGCACGTGTAAAAGAAGAAACAGGAGGAAAAGgtcaataccaagttaatacacggatact
tttcttttgtcttgcacagagaacaaatctctttaccaaattcaatcaatgtttgttgcagGTGTTGATGTAATATTAGATAATATGGGCGGCTCTTATCTTCAGAGGAATTTAGATGCCTTGGGCCTCGATGGGAGATTATTTCTCATTGGCTTTCAAGGGGGTTCTGTTGCACAGATCAACTTATCAACTATATTTGC
AAAGCGCCTGACTATCCAAGCTGCGGGCTTGCGGTCTCGCTCTGCAGAGAACAAAGAACAAATCATCTCTGATGTTCACAAGAATGTTTGGCCTGCATTTGAAGAAGGAAAAGTGAAGCCCATCATTTACACCTCACTTCCCTTGTCACAAGCTGCAGAGGCCCACAGGATTATGGAATCCAGCACACATATTGGCAAAA
TCCTGCTTGTAGTTTAGCAATTGTATGTGAAGAGGGATGGAATAAAGATATTCTCCCCATCTCAATTCTTTTTTTATATATTCAGAGGATAGCTAAAGTGAAACGTCTTACACACTATCGAATGCCTTTTATTATCTATTGTTTGAAGTTATGGGAAATCCATTAGTCTAAAAATTTCTTGCTGGGAACGCTGGCTATTT
GTAATTCTGCTTAAGCTATTTTAGAATGGTTGAAAACTCGTGGACTATTTGAATTAATAGTAGGTGAATGTGTCTTTGCATGAGTGAGAGAGAAAGAATGAGAGATTTTTTTTACTAACATCTGTGTCACCAAACAATGTATTGTACTTGTAACAAAGGATTACAGTAAAATAAAAATCATCATTGAGTGGTTAGATTTA
AACATGAAACATAGCCAAGGGATTAGTTAAACCAGTTATTGTCAATGTTGCTGGGCATTTGTCTAGTTCTATGCCAGCAAGTGCTCAGTTTGATTTTTTTGGGTCAGGTACACTAGTCAATGCTGAC

Intron 5 GTTTGCACTA ... TGATATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.05	15	2	6
A2	10	18	2	2
A3	44	7	5	6
A4	2.22	22	1	0
A5	12.5	7	0	2
A6	17.24	24	7	3
A7	33.33	18	7	11
A8	30.23	30	8	18
A9	23.53	26	4	12
A10	19.3	23	4	7

>TRINITY_DN107866_c0_g2_i8 from sample A3 / Example spliced isoform

CCTCATGTCCCTTAGTTAAGGGCTTACTTCCAATGTGGTCGGCGATCTGCCTCATAAGTTTTTCAAGAACGACGACGTGATATTGACCCTCCATTGCGAAATTTGCACAAAACTATTTTGTACTTTTGAAAAAATGCCTCGAGCTCCAGGGGAATGGAGCGGAGAGAGGGCTAAGTTCTGATTTACAGAATATATAAACG
AAAGCAAGCAGATGGACTGATCAAGAACAGGACATTGATTTCTCGAATAATTCTCATGGATTTTATCTGATTTGGTTAGTCAGATTATAGATAATACATATGAATGTCTCTTCACATCTGCATCTCATGCTAATACTCTGCCTCTGCTACAGACCGTAGTGGGCAATTTCTTTGTGCGGCAAACCTCAACCAAGGAGGAT
TTTTAGTCCGTTAGATTGCTTAAGCCTCCCAAGTTGTGTAATTTTTTCCCCCCGTATTTTTTTTGGTTGTTTTGAAGGTTGTAAATTATGAATCCCTTGCATTGCCAAGATACTAGACATGTGGGGGGCAAGTCCCTGGGTGATGTGATTACATCCTCCTCCTCAGGCCTCACGCCTGTACTAATATCTCTGATGTAATG
TCATGCTTTGTGAGCCATCTTTAGCTTCTGATTATTTTGCGATTATTATCTATTAGATGTTTGAAAGTTTATCCATATGTTGGAATTTCATTCCACACGAATACTTGATTTGATGTTTAAAAGTCTATCCCTATGTTGGAATTTCATTCCACATGAATACTTCATGTGTTTCTGGGCAGAGTTTGGTATTTTTGACATAG
GAGTAAATATGACAACCATAGGAGTCCGAGTTCAGCAGCTTCTTAGTCACCCATCTGGCTGTTTTGGGTTTTTAAGCTACCCAGTTGGCTGTTCTGGTACTTCCAAGCTTCTACTTGTGCAAGCTGTAGGTGCTCTTTTGAATAGTGAAAAGAAGTCATTCTTCTGACTAGTTGTTATATACTGGTGTGGAAATACATTG
TATTCCGTGCCCTTGAGCTTAGCCTTGATCTGTTAAATGAAAGGTTAGCCTCAGCAAGGAGTGAATTTAGTATGTGCATGTGCAGGGAAGAGGAGAACCAGAGAGTGAGAAAGAGAGAGAGATAGGGAGAGAGAGAGGGAGAGAAAGGAATGAGAGGAGAGACAGGCTCAAACACAACTCGGACACTTATATTATTGCAA
CATTTTCCACAATGACTGGCAATAGAAAGCTTGTGGTTTTAGGCATTCCCTGGGATGTGGATACTGAAGGCTTGAGACAATACATGTGCCAATTTGGTGACTTGGAGGATGTAATTGTAATGAAGGATCGTTTATCGGGACGATCTCGTGGATTTGGATATGTTACCTTCTCCTCTTATGAAGGTGCAGAGAAAGCAGTG
GCGAGTCAACATGTTTTACAAGGGAGGATTCTTGAGGTCAAAATAGCAACCCCAAAGGATGAGATGAGGCCCACAAGTAGGAAAATCACTCGCATATTTGTTGCGCGAATCTCTCCAACTGTTACAGATGAAATATTTCGAAGCTACTTCGAGAAATATGGCTCCATTCTAGATGCTTATTTGCCAAAGGACCAAACAAC
AAAGCAACATCGTGGAATTGGATTTGTTACATATGAAAATGCAGAGTCTGTTGACAAAGTTATGTCAGAGAGTCATGAGCTCGCGGGATCAACTATTGCTGTAGATCGAGCAAATCCAAAGTGTGATACCCTGAAGGAGGAGACTGGAAAAGTTTGGGAAAGGACCTTTCCTGGAGCCAGTGGTGTTTTTAACAGTCATG
TAAACACAACAGACTTTGGACCTTTTTATGTGTCAGCTTTTGGAGGTTGTGACTTCCCAGGATCAGATAGAGCGGCTATAGGAAATACATATGAAAGCAACTGTGGAAGAACCAACCCATGGTCAGCAGGTCTACCTGCACCACAAGTTGGAGGACCTTTGCCAGGAATACCGGGTATTCATCCATCAGCCATGAATTAT
GGCTCAACTGATCCTAATTTTGCTCCGTCCAGAGTGGGGAGAAAAATATTTGTAGGGCGTATTCCAGTTGAAGCAACAGCTGAAGACTTGCAATTATATTTCAGTCAATTTGGTCAGATTTTGGATGTGTATCTTCCAAAGGATGCAAAGAAAATATCCCATAGAGGTTTTGGTTTTGTGACTTTTGCGGAAGAGTCTAC
TGCTGAGCATGTTTCTCAGAGGAGACATGAGATCCTTGGGCACTTGATTGCAGTTGATCGTGCTTCACCATTGGATGAAACGCCAAGTGTAGGAGTCTATGCTCCTTCTGGAATGGGAAAAAAGATTTTCATTGGTCGCATTCCAGTTGAAGCAACGACTGAAGACTTGAGACTGTATTTTAGCCAGTATGGGTGCATTT
TAGATGTGTACCTTCCAAAGGATGCAAAGAAAATCTCCCACAGAGGATTTGGCTTTGTCACCTTTGCAGAAGAGTCTACAGCTGAGCATGTTGCTCAAAAGACACATGACATTCTTGGGCATCGGATTGCAGTTGATCGTGCAGCACCTTTGGACGAGACACGGAGTGGATTAAAGCCATCGAATGCTTTATTTGGAGGT
GCTGGTGGTCCACTAAGAGGATCTAGCTCTACCATCTCTGGAAGTGCTTATGGAAACACACCTGCTAACCTTGGTTTTAATAGTAGTTGGACTCCATATGGTGGGTTGGGACAAATGGCTGCTTCTGATGGAGGTCCAAGTGGTCATCGGACTTCCAGGATGGAACCCCGTTACAGGCCGTACTAGGATAGAATTGTTGT
GTAAAGTGATGTTAAATTGCCATCATTTTCTTGGGGCTTTTGGAAGATGCTTTTGAAGTTAGATCATTGACATACCTGTCATCATTAAAGAAATTATTTCTGATGCTTTGGTTTATTAATGATGTGCTTTGATTGGAGTGTTAATAACTGTTAGATAGGAAGTTTGGGCTCATATAATTTGCTTGTTTTGCCTATATTTT
GATTTTTTCTATTCATATGGCAATTGACTCAATAATCTGGACTCATCATTCAGACGGTAGCAAGTTTGCTAACCATTGCCACTAATTGATGGTGAAGTTAGTTATTCTAACT

>TRINITY_DN107866_c0_g2_i13 from sample A3 / Example unspliced isoform:

CATTATCCAAAAAGTAATTTTGGCCCCGTTCAAACAAGCGTTTGATGATGTCTGGGCTTCCCATTTCATGATGAGATCATGGCTGAGCTCAAAATTAAATCCGTGGAGAGTTTCCAATGAGATCACGGCTTAAAATAAAATCGACAACTTTCTAATCGAAATCCTCCATCTAATGGCGCCGTCACATAACATAAAACACC
GCAAGCTTGGATTCTCGCCTTCTAACATCCGACACTCAGTTTCCATAGCTTGAATTGCAGGGAAGACGAACAAAATGAAGGCAGTGGTGATAACAACTCCAGGAGATGCAGATGTTCTGCAATTACAAGATGTAGATGACCCCCAAATCAACGACAATGAAGTGCTGATAAAAATCTCGGCAACAGCCCTGAATAGGGCA
GATGCCCTACAAAGACAAGGGAGGTACTCTCCGCCGAAAGACGCACCGCCTTATCTCGGTGTCGAATGCTCAGGCATTATTGAAGCCGTCGGCAAAAATGTACAGAACTGGAAAGTTGGCGATGAGTGTGATACCCTGAAGGAGGAGACTGGAAAAGTTTGGGAAAGGACCTTTCCTGGAGCCAGTGGTGTTTTTAACAG
TCATGTAAACACAACAGACTTTGGACCTTTTTATGTGTCAGCTTTTGGAGGTTGTGACTTCCCAGGATCAGATAGAGCGGCTATAGGAAATACATATGAAAGCAACTGTGGAAGAACCAACCCATGGTCAGCAGGTCTACCTGCACCACAAGTTGGAGGACCTTTGCCAGGAATACCGGGTATTCATCCATCAGCCATGA
ATTATGGCTCAACTGATCCTAATTTTGCTCCGTCCAGAGTGGGGAGAAAAATATTTGTAGGGCGTATTCCAGTTGAAGCAACAGCTGAAGACTTGCAATTATATTTCAGTCAATTTGGTCAGATTTTGGATGTGTATCTTCCAAAGGATGCAAAGAAAATATCCCATAGAGGTTTTGGTTTTGTGACTTTTGCGGAAGAG
TCTACTGCTGAGCATGTTTCTCAGAGGAGACATGAGATCCTTGGGCACTTGATTGCAGTTGATCGTGCTTCACCATTGGATGAAACGCCAAGTGTAGGAGTCTATGCTCCTTCTGGAATGGGAAAAAAGATTTTCATTGGTCGCATTCCAGTTGAAGCAACGACTGAAGACTTGAGACTGTATTTTAGCCAGTATGGGTG
CATTTTAGATGTGTACCTTCCAAAGgtttgcactattttgagtactaactctggctgttgataatctcttttgccctcgacggctgagatattttccttctagtaaaaaattctattggtttatttgtatgatatgcagGATGCAAAGAAAATCTCCCACAGAGGATTTGGCTTTGTCACCTTTGCAGAAGAGTCTACAG
CTGAGCATGTTGCTCAAAAGACACATGACATTCTTGGGCATCGGATTGCAGTTGATCGTGCAGCACCTTTGGACGAGACACGGAGTGGATTAAAGCCATCGAATGCTTTATTTGGAGGTGCTGGTGGTCCACTAAGAGGATCTAGCTCTACCATCTCTGGAAGTGCTTATGGAAACACACCTGCTAACCTTGGTTTTAAT
AGTAGTTGGACTCCATATGGTGGGTTGGGACAAATGGCTGCTTCTGATGGAGGTCCAAGTGGTCATCGGACTTCCAGGATGGAACCCCGTTACAGGCCGTACTAGGATAGAATTGTTGTGTAAAGTGATGTTAAATTGCCATCATTTTCTTGGGGCTTTTGGAAGATGCTTTTGAAGTTAGATCATTGACATACCTGTCA
TCATTAAAGAAATTATTTCTGATGCTTTGGTTTATTAATGATGTGCTTTGATTGGAGTGTTAATAACTGTTAGATAGGAAGTTTGGGCTCATATAATTTGCTTGTTTTGCCTATATTTTGATTTTTTCTATTCATATGGCAATTGACTCAATAATCTGGACTCATCATTCAGACGGTAGCAAGTTTGCTAACCATTGCCA
CTAATTGATGGTGAAGTTAGTTATTCTAACT

Intron 6 GTTTGATTTT ... AACCTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	84.21	3	17	15
A2	100	0	7	8
A3	88.57	2	11	20
A4	100	0	12	13
A5	100	0	8	5
A6	93.1	1	13	14
A7	85.19	2	9	14
A8	77.78	7	22	27
A9	100	0	21	14
A10	84.91	4	17	28

>TRINITY_DN44623_c0_g1_i4 from sample A10 / Example spliced isoform

CTGCGGGAAGCTTCTGCTCTGAAGGCTATCACTCGGAGGGGAAATTGACATGCTGCTCTGGAAGGACCTCTACAGGATGCTGCTCGAACGCTCTGAAACAGAAGAATAAAAAAATACGAGGGTTATGGCAATGCAACGAAGAAAAAAAGTCCGTGTTACTCCAGTCCACGTTTGTGCACTTCTGGGAGGAGGAGGGTATG
CAGAAAAGGTGGCCGTTCCATCAGGGCATGTCTTGCCTGTTCCAAAAGGCATTTCATTACAAGAAGCTGCAGCCCTTCCTGAGGTGGCATGCACTGTGTGGTCTACAGTTTTTATGACAAGTCATCTGTCCTCAGGCGAGAGCATTCTGGTGCATGGAGGCTCTAGTGGTATTGGAACGTTTGCTATTCAGATAGCCAAG
TACTATGGAGCAAGAGTGTTTGTTACAGCTGGAAACGAGGAAAAACTAGCAAGTTGTAAGGAGCTTGGTGCTGATGTGGCAATAAATTACAAAACAGAAGATTTTGTTGCACGTGTAAAAGAAGAAACAGGAGGAAAAGGTGTTGATGTAATATTAGATAATATGGGCGGCTCTTATCTTCAGAGGAATTTAGATGCCTT
GGGCCTCGATGGGAGATTATTTCTCATTGGCTTTCAAGGGGGTTCTGTTGCACAGATCAACTTATCAACTATATTTGCAAAGCGCCTGACTATCCAAGCTGCGGGCTTGCGGTCTCGCTCTGCAGAGAACAAAGAACAAATCATCTCTGATGTTCACAAGAATGTTTGGCCTGCATTTGAAGAAGGAAAAGTGAAGCCCA
TCATTTACACCTCACTTCCCTTGTCACAAGCTGCAGAGGCCCACAGGATTATGGAATCCAGCACACATATTGGCAAAATCCTGCTTGTAGTTTAGCAATTGTATGTGAAGAGGGATGGAATAAAGATATTCTCCCCATCTCAATTCTTTTTTTATATATTCAGAGGATAGCTAAAGTGAAACGTCTTACACACTATCGAA
TGCCTTTTATTATCTATTGTTTGAAGTTATGGGAAATCCATTAGTCTAAAAATTTCTTGCTGGGAACGCTGGCTATTTGTAATTCTGCTTAAGCTATTTTAGAATGGTTGAAAACTCGTGGACTATTTGAATTAATAGTAGGTGAATGTGTCTTTGCATGAGTGAGAGAGAAAGAATGAGAGATTTTTTTTACTAACATC
TGTGTCACCAAACAATGTATTGTACTTGTAACAAAGGATTACAGTAAAATAAAAATCATCATTGAGTGGTTAGATTTAAACATGAAACATAGCC

>TRINITY_DN44623_c0_g1_i2 from sample A10 / Example unspliced isoform:

CTGCGGGAAGCTTCTGCTCTGAAGGCTATCACTCGGAGGGGAAATTGACATGCTGCTCTGGAAGGACCTCTACAGGATGCTGCTCGAACGCTCTGgtttgattttctcttctctgctatgtcctcctaatttaatataattcattagcaatttgtcagggctcagctttgtttacaacctatcagAAACAGAAGAATAAA
AAAATACGAGGGTTATGGCAATGCAACGAAGAAAAAAAGTCCGTGTTACTCCAGTCCACGTATGTATGCTTCTCCTTCTCCTCCTGTCTGCGTTTTGTATTTTTTGTTGGTCGAATCAAATTTAATCTAAGAATGAGTTTCTACCCACCTCTTTATTTTTAGACCAACAAACTGTGCAACTTTTATCTACTCTATTATTA
ACCCTGTCATTTCCGGTATATCTCTTTTGTTTTTCCGCA