sp|Q9FM82|GGLO4_ARATH ----> Probable L-gulonolactone oxidase 4

Intron 1 GTGCATATGG ... TTAATCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.52	55	32	21
A2	31.97	50	20	27
A3	20.48	66	21	13
A4	27.59	42	22	10
A5	33.33	37	8	29
A6	16.22	31	10	2
A7	15.46	41	14	1
A8	23.08	25	13	2
A9	17.54	47	13	7
A10	23.64	42	23	3

>TRINITY_DN121564_c0_g3_i2 from sample A2 / Example spliced isoform

TGAGCCATCCAGTTATCTTGTTTGATCTATGGATTGTATATGAGAGGGGAAATCGTGCCAGACTCACATTTGGGGAAATCATGGCAGAATCACACACGGAAAAATCTTTGGATCACATAAGAAAATGCGGCATGGAGTTCAATTGGTGAACCTGATAATTGAAGCAATTAAGGAACTCTGCTTTTCATGGCTTCTCAGCG
AATGCGACTGATATATTTGAGCAGGAAAATCGAAAACAGCTGGATCGTCTAAAGAAGAAAATGAGACAGGTAAATAAACCTACCGTTTCCCTCTCATAGCTTCCTAATGCAATAGAAACACAAAATCCATGTTTATAATGTAATAATTTCTTCACTATTTTTCTGTTTTGATTCAATTGTAATTGAAATTTGTCAATGAA
AATTCTCCCTTCATTCTGATTTGATTTTTGTTGTTTTAATCTTTATTTCACAAGAAGGAACCGTTGAGTTGATGTCTTTGGTGGCTCTAATTTTCTTTCCTTCCTTTTCCCTCATTTGATTATTTAATACTTTGCATTTTCAAATGGAATTTTAATTC

>TRINITY_DN121564_c0_g3_i4 from sample A2 / Example unspliced isoform:

TGAGCCATCCAGTTATCTTGTTTGATCTATGGATTGTATATGAGAGGGGAAATCGTGCCAGACTCACATTTGGGGAAATCATGGCAGAATCACACACGGAAAAATCTTTGGATCACATAAGAAAATGCGGCATGGAGTTCAATTGGTGAACCTGATgtgcatatggcttcgttccttctcgttatttctttcatttgatt
attttatatgatgaatcttgcattttaattcatcaatcgaaatttgattcctaacttttttcaatttgtggtgttaatttttaatcacagAATTGAAGCAATTAAGGAACTCTGCTTTTCATGGCTTCTCAGCGAATGCGACTGATATATTTGAGCAGGAAAATCGAAAACAGCTGGATCGTCTAAAGAAGAAAATGAGA
CAGGTAAATAAACCTACCGTTTCCCTCTCATAGCTTCCTAATGCAATAGAAACACAAAATCCATGTTTATAATGTAATAATTTCTTCACTATTTTTCTGTTTTGATTCAATTGTAATTGAAATTTGTCAATGAAAATTCTCCCTTCATTCTGATTTGATTTTTGTTGTTTTAATCTTTATTTCACAAGAAGGAACCGTTG
AGTTGATGTCTTTGGTGGCTCTAATTTTCTTTCCTTCCTTTTCCCTCATTTGATTATTTAATACTTTGCATTTTCAAATGGAATTTTAATTC

Intron 2 GTAAATAAAC ... AATTGAGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	84	2	14	7
A2	51.85	13	13	15
A3	51.72	14	14	16
A4	100	0	11	9
A5	70	3	11	3
A6	24.14	22	5	9
A7	33.33	31	8	23
A8	26.83	15	2	9
A9	34.69	32	11	23
A10	29.73	26	10	12

>TRINITY_DN48859_c0_g1_i8 from sample A7 / Example spliced isoform

TGTATATGAGAGGGGAAATCGTGCCAGACTCACATTTGGGGAAATCATGGCAGAATCACACACAGAAAAATCTTTGGATCACATAAGAAAATGCGGCATGGAGTTCAATTGAATTGAAGCAATTAAGGAACTCTGCTTTTCATGGCTTCTCAGCGAATGCGACTGATATATTTGAGCAGGAACATCGAAAACAGCTGGAT
CGTCTAAAGAAGAAAATGAGACAGGCACGGCGGCGTCTGCACAGCTGGATCGTCTACACATCTGCATCGCCTACACACCCAACTGCAAAAGAAAATGAAACAGCTGGATCGTCTACACACCCAAATGCAATAGAAAATGAAACAGGGTTACTCCTTCCCACTCTTAAAATTTG

>TRINITY_DN48859_c0_g1_i10 from sample A7 / Example unspliced isoform:

TGTATATGAGAGGGGAAATCGTGCCAGACTCACATTTGGGGAAATCATGGCAGAATCACACACAGAAAAATCTTTGGATCACATAAGAAAATGCGGCATGGAGTTCAATTGGTGAACCTGATAATTGAAGCAATTAAGGAACTCTGCTTTTCATGGCTTCTCAGCGAATGCGACTGATATATTTGAGCAGGAACATCGAA
AACAGCTGGATCGTCTAAAGAAGAAAATGAGACAGgtaaataaacctaccgtttccctctcatagcttcctaatgcaatagaaacacaaaatccatgtttataatgtaataatttcttcactatttttctgttttgattcaattgtaattgaaatttgtcaatgaaaattctcccttcattctgatttgatttttgttgt
tttaatctttatttcacaagaaggaaccgttgagttgatgtctttggtggctctaattttctttccttcctttccctgatttccctgatttgaaacaattgaggagGCACGGCGGCGTCTGCACAGCTGGATCGTCTACACATCTGCATCGCCTACACACCCAACTGCAAAAGAAAATGAAACAGCTGGATCGTCTACAC
ACCCAAATGCAATAGAAAATGAAACAGGAGTTTTTGTTTAACCTCACCACCAAAGATCTTGTCTCCCTTTTTGTACGCTTAGATTATACTGACCG

Intron 3 GTAAATAAAC ... AACAATTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	14	5
A2	52.85	12.5	13	15
A3	53.64	13	14	16
A4	100	0	11	9
A5	70	3	11	3
A6	24.57	21.5	5	9
A7	33.33	30	8	22
A8	26.32	14	2	8
A9	35.17	29.5	11	21
A10	30.14	25.5	10	12

>TRINITY_DN48859_c0_g1_i4 from sample A7 / Example spliced isoform

TGTATATGAGAGGGGAAATCGTGCCAGACTCACATTTGGGGAAATCATGGCAGAATCACACACAGAAAAATCTTTGGATCACATAAGAAAATGCGGCATGGAGTTCAATTGGTGAACCTGATAATTGAAGCAATTAAGGAACTCTGCTTTTCATGGCTTCTCAGCGAATGCGACTGATATATTTGAGCAGGAACATCGAA
AACAGCTGGATCGTCTAAAGAAGAAAATGAGACAGGAGGCACGGCGGCGTCTGCACAGCTGGATCGTCTACACATCTGCATCGCCTACACACCCAACTGCAAAAGAAAATGAAACAGCTGGATCGTCTACACACCCAAATGCAATAGAAAATGAAACAGGTACATCGTTTCCCTTTCATATCTTCCTAATGCAATGGAAT
A

>TRINITY_DN48859_c0_g1_i10 from sample A7 / Example unspliced isoform:

TGTATATGAGAGGGGAAATCGTGCCAGACTCACATTTGGGGAAATCATGGCAGAATCACACACAGAAAAATCTTTGGATCACATAAGAAAATGCGGCATGGAGTTCAATTGGTGAACCTGATAATTGAAGCAATTAAGGAACTCTGCTTTTCATGGCTTCTCAGCGAATGCGACTGATATATTTGAGCAGGAACATCGAA
AACAGCTGGATCGTCTAAAGAAGAAAATGAGACAGgtaaataaacctaccgtttccctctcatagcttcctaatgcaatagaaacacaaaatccatgtttataatgtaataatttcttcactatttttctgttttgattcaattgtaattgaaatttgtcaatgaaaattctcccttcattctgatttgatttttgttgt
tttaatctttatttcacaagaaggaaccgttgagttgatgtctttggtggctctaattttctttccttcctttccctgatttccctgatttgaaacaattgagGAGGCACGGCGGCGTCTGCACAGCTGGATCGTCTACACATCTGCATCGCCTACACACCCAACTGCAAAAGAAAATGAAACAGCTGGATCGTCTACAC
ACCCAAATGCAATAGAAAATGAAACAGGAGTTTTTGTTTAACCTCACCACCAAAGATCTTGTCTCCCTTTTTGTACGCTTAGATTATACTGACCG

Intron 4 GCGAACCTGA ... TTAATCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	34.55	47.3	30	21
A2	34.29	49.7	23.7	28.3
A3	22.56	61	23	13
A4	31.77	36.7	24.3	10
A5	35.26	37	11.3	29
A6	16.94	35.3	12.7	2
A7	15.5	39	14	1
A8	17.72	34.7	13.3	2
A9	16.25	47.7	11.7	7
A10	19.8	52.7	24	3

>TRINITY_DN121564_c0_g3_i6 from sample A2 / Example spliced isoform

TGAGCCATCCAGTTATCTTGTTTGATCTATGGATTGTATATGAGAGGGGAAATCGTGCCAGACTCACATTTGGGGAAATCATGGCAGAATCACACACGGAAAAATCTTTGGATCACATAAGAAAATGCGGCATGGAGTTCAATTGAATTGAAGCAATTAAGGAACTCTGCTTTTCATGGCTTCTCAGCGAATGCGACTGA
TATATTTGAGCAGGAAAATCGAAAACAGCTGGATCGTCTAAAGAAGAAAATGAGACAGGTAAATAAACCTACCGTTTCCCTCTCATAGCTTCCTAATGCAATAGAAACACAAAATCCATGTTTATAATGTAATAATTTCTTCACTATTTTTCTGTTTTGATTCAATTGTAATTGAAATTTGTCAATGAAAATTCTCCCTT
CATTCTGATTTGATTTTTGTTGTTTTAATCTTTATTTCACAAGAAGGAACCGTTGAGTTGATGTCTTTGGTGGCTCTAATTTTCTTTCCTTCCTTTTCCCTCATTTGATTATTTAATACTTTGCATTTTCAAATGGAATTTTAATTC

>TRINITY_DN121564_c0_g3_i4 from sample A2 / Example unspliced isoform:

TGAGCCATCCAGTTATCTTGTTTGATCTATGGATTGTATATGAGAGGGGAAATCGTGCCAGACTCACATTTGGGGAAATCATGGCAGAATCACACACGGAAAAATCTTTGGATCACATAAGAAAATGCGGCATGGAGTTCAATTGgtgaacctgatgtgcatatggcttcgttccttctcgttatttctttcatttgatt
attttatatgatgaatcttgcattttaattcatcaatcgaaatttgattcctaacttttttcaatttgtggtgttaatttttaatcacagAATTGAAGCAATTAAGGAACTCTGCTTTTCATGGCTTCTCAGCGAATGCGACTGATATATTTGAGCAGGAAAATCGAAAACAGCTGGATCGTCTAAAGAAGAAAATGAGA
CAGGTAAATAAACCTACCGTTTCCCTCTCATAGCTTCCTAATGCAATAGAAACACAAAATCCATGTTTATAATGTAATAATTTCTTCACTATTTTTCTGTTTTGATTCAATTGTAATTGAAATTTGTCAATGAAAATTCTCCCTTCATTCTGATTTGATTTTTGTTGTTTTAATCTTTATTTCACAAGAAGGAACCGTTG
AGTTGATGTCTTTGGTGGCTCTAATTTTCTTTCCTTCCTTTTCCCTCATTTGATTATTTAATACTTTGCATTTTCAAATGGAATTTTAATTC

Intron 5 GTAAATAAAC ... ATTTCACAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.14	5	14	32
A2	83.87	5	13	39
A3	95.35	1	14	27
A4	93.33	1	11	17
A5	90.91	1	11	9
A6	39.39	10	5	8
A7	42.86	14	8	13
A8	78.57	3	2	20
A9	44.44	20	11	21
A10	45.45	9	10	5

>TRINITY_DN48859_c0_g1_i5 from sample A7 / Example spliced isoform

TGTATATGAGAGGGGAAATCGTGCCAGACTCACATTTGGGGAAATCATGGCAGAATCACACACAGAAAAATCTTTGGATCACATAAGAAAATGCGGCATGGAGTTCAATTGAATTGAAGCAATTAAGGAACTCTGCTTTTCATGGCTTCTCAGCGAATGCGACTGATATATTTGAGCAGGAACATCGAAAACAGCTGGAT
CGTCTAAAGAAGAAAATGAGACAGAAGGAACCGTTGAGTTGATGTCTTTGGTGGCTCTAATTTTCTTTCCTTCCTTTCCCTGATTTCCCTGATTTGAAACAATTGAGGAGGCACGGCGGCGTCTGCACAGCTGGATCGTCTACACATCTGCATCGCCTACACACCCAACTGCAAAAGAAAATGAAACAGCTGGATCGTCT
ACACACCCAAATGCAATAGAAAATGAAACAGGTACATCGTTTCCCTTTCATATCTTCCTAATGCAATGGAATA

>TRINITY_DN48859_c0_g1_i10 from sample A7 / Example unspliced isoform:

TGTATATGAGAGGGGAAATCGTGCCAGACTCACATTTGGGGAAATCATGGCAGAATCACACACAGAAAAATCTTTGGATCACATAAGAAAATGCGGCATGGAGTTCAATTGGTGAACCTGATAATTGAAGCAATTAAGGAACTCTGCTTTTCATGGCTTCTCAGCGAATGCGACTGATATATTTGAGCAGGAACATCGAA
AACAGCTGGATCGTCTAAAGAAGAAAATGAGACAGgtaaataaacctaccgtttccctctcatagcttcctaatgcaatagaaacacaaaatccatgtttataatgtaataatttcttcactatttttctgttttgattcaattgtaattgaaatttgtcaatgaaaattctcccttcattctgatttgatttttgttgt
tttaatctttatttcacaagAAGGAACCGTTGAGTTGATGTCTTTGGTGGCTCTAATTTTCTTTCCTTCCTTTCCCTGATTTCCCTGATTTGAAACAATTGAGGAGGCACGGCGGCGTCTGCACAGCTGGATCGTCTACACATCTGCATCGCCTACACACCCAACTGCAAAAGAAAATGAAACAGCTGGATCGTCTACAC
ACCCAAATGCAATAGAAAATGAAACAGGAGTTTTTGTTTAACCTCACCACCAAAGATCTTGTCTCCCTTTTTGTACGCTTAGATTATACTGACCG