sp|P38385|SC61G_ORYSJ ----> Protein transport protein Sec61 subunit gamma

Intron 1 GTAAGGTGGA ... CAATTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	9.2	133.3	13.5	10.3
A2	12.06	176.8	24	12
A3	10.29	212.3	24.3	16.8
A4	14.83	106.5	18.5	17.3
A5	8.7	136.3	13	9.8
A6	5.44	2170.5	124	71.5
A7	6.86	1367.8	100.3	48.5
A8	4.95	1062	55	37.5
A9	7.32	1375	108.3	49.3
A10	6.39	1086.8	73.8	24

>TRINITY_DN114333_c0_g1_i6 from sample A1 / Example spliced isoform

GGAGGAAGTAAACACCAGGGTTTTGATTTTTGCGCAAGGGTCTGTTTCATATCTGAAGGTGGTGGGAGCGGATTTGCGGCGGGCAGCTATATTGTGAGCAGCATATAGAGAAAGCTTGTGTTGGCACACCAGGCGCGATAAAAATGGAAGCCGTGGACGCCGTTGTAAGGCCGTTGCAGGAATTTGCCAAGGATAGCGTG
CGCCTTGTCAAAAGATGCCACAAGCCTGATCGCAAAGAGTTTAGTAAAGTGGCTTTCAGGACGGCCATTGGATTTGTTGTTATGGGATTTGTTGGTTTCTTTGTCAAGTTGATCTTCATTCCAATCAACAACATCATAGTGGGATCAGGATAGG

>TRINITY_DN114333_c0_g1_i1 from sample A1 / Example unspliced isoform:

GGAGGAAGTAAACACCAGGGTTTTGATTTTTGCGCAAGGGTCTGTTTCATATCTGAAGGTGGTGGGAGCGGATTTGCGGCGGGCAGCTATATTGTGAGCAGCATATAGAGgtaaggtggagctattatgcatcagtatatgtcaatttcaatcagatctgttaagaaagtgagtccaacaattcacagAAAGCTTGTGTT
GGCACACCAGGCGCGATAAAAATGGAAGCCGTGGACGCCGTTGTAAGGCCGTTGCAGGAATTTGCCAAGGATAGCGTGCGCCTTGTCAAAAGATGCCACAAGCCTGATCGCAAAGAGTTTAGTAAAGTGGCTTTCAGGACGGCCATTGGATTTGTTGTTATGGGATTTGTTGGTTTCTTTGTCAAGTTGATCTTCATTCC
AATCAACAACATCATAGTGGGATCAGGATAGG

Intron 2 GTAAGATCTT ... AAATTGTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	37	37	14
A2	51	24.5	25.5	8
A3	59.46	30	44	4
A4	49.06	27	26	12
A5	53.33	21	24	3
A6	50.81	317	327.5	48
A7	49.83	214.5	213	54
A8	50.2	185	186.5	51
A9	50.61	223.5	229	65
A10	50.41	148	150.5	29

>TRINITY_DN40117_c0_g1_i3 from sample A7 / Example spliced isoform

GAGAGAATCACTCCGGAGTCCGGAGGGCGGACTTGAAACAGATTCCAAAACAGTATGTCCTTGAATAAACAACCAGGAATGTTATATAAGTCTCAGAGGCCAAAGGTCTTATGAGAGGGGAGGAAGTAAACACCAGGGTTTTGATTTTTGCGCAAGGGTTTGTTTCATATCTGAAGGTGGTGGGAGCGGATTTACGGCGG
GCAGCTATATCGTGAGCAGCATATAGAGGCGCGATAAAAATGGAAGCCGTGGACGCCGTTGTAAGGCCGTTGCAGGAATTTGCCAAGGATAGCGTGCGCCTTGTCAAAAGATGCCACAAGCCTGATCGCAAAGAGTTTAGTAAAGTGGCTTTCAGGACGGCCATTGGATTTGTTGTCATGGGATTTGTTGGTTTCTTTGT
CAAGTTGATCTTCATTCCAATCAACAACATCATAGTGGGATCAGGATAGATGAAACAGACTATAATGAAGCAGAAGCAAGAACTATGGCTGAAGTGATATCTCACCAACAATTAAGGTGGTTGAAAGAGTCTTTGAATTAATTTAACATGATCTCGGTCATTTTAAGAATTTTGAATGGTATTTGAGCACAGAAATGAAT
GTGCATTTCTTATATGTTCAAATTGAGCTTATATTGATTTTATGATCCCTTTGGAGGGTGTCTCTATGTTTTCTAAGTTTTTTCTCTGACATATATTGTTTGCATTCATGAGGATTGCTTTTATTTTACTTGTTAATATTTGAAGAATCAACCTGCACCCCA

>TRINITY_DN40117_c0_g1_i1 from sample A7 / Example unspliced isoform:

TGGACGCCGTTGTAAGGCCGTTGCAGGAATTTGCCAAGGATAGCGTGCGCCTTGTCAAAAGATGCCACAAGCCTGATCGCAAAGAGTTTAGTAAAGTGGCTTTCAGGACGGCCATTGGATTTGTTGTCATGGGATTTGTTGGTTTCTTTGTCAAGTTGATCTTCATTCCAATCAACAACATCATAGTGGGATCAGGATAG


gtaagatcttttttttttaaattattttcaacaattaatggtgaattaaaagcaaaatagatattcttaaaaaatttctatgatgtactgaaaggcactagaattggaaaattgtaagATGAAACAGACTATAATGAAGCAGAAGCAAGAACTATGGCTGAAGTGATATCTCACCAACAATTAAGGTGGTTGAAAGAGTC
TTTGAATTAATTTAACATGATCTCGGTCATTTTAAGAATTTTGAATGGTATTTGAGCACAGAAATGAATGTGCATTTCTTATATGTTCAAATTGAGCTTATATTGATTTTATGATCCCTTTGGAGGGTGTCTCTATGTTTTCTAAGTTTTTTCTCTGACATATATTGTTTGCATTCATGAGGATTGCTTTTATTTTACTT
GTTAATATTTGAAGAATCAACCTGCACCCCA

Intron 3 GTAAGCTCTT ... AAATTGTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	37.5	37.5	14
A2	51.65	22.5	24	8
A3	59.73	30	44.5	4
A4	50	26.5	26.5	12
A5	53.41	20.5	23.5	3
A6	50.35	318.5	323	48
A7	49.7	214	211.5	54
A8	50.4	184.5	187.5	51
A9	50.38	226	229.5	65
A10	50.25	149	150.5	29

>TRINITY_DN38826_c0_g1_i4 from sample A6 / Example spliced isoform

TCAAAGTGAGAAAATCACTCCGGAGGGCGGACTTGAAACAGATTCCAAAACAGTATGTCCTTGAATAAACAACCAGGAATGTTATATAAGTCTCAGAGGCCAAAGGTCTTATGAGAGGGGAGGAAGTAAACACCAGGGTTTTGATTTTTGCGCAAGGGTCTGTTTCATATCTGAAGGTGGTGGGAGCGGATTTGCGGCGG
GCAGCTATATTGTGAGCAGCATATAGAGGCGCGATAAAAATGGAAGCCGTGGACGCCGTTGTAAGGCCGTTGCAGGAATTTGCCAAGGATAGCGTGCGCCTTGTCAAAAGATGCCACAAGCCTGATCGCAAAGAGTTTAGTAAAGTGGCTTTCAGGACGGCCATTGGATTTGTTGTTATGGGATTTGTTGGTTTCTTTGT
CAAGTTGATCTTCATTCCAATCAACAACATCATAGTGGGATCAGGATAGATGAAACAGACTATAATGAAGCAGAAGCAAGAACTATGGCTGAAGTGATATCTCACCAACAATTAAGGTGGTTGAAAGAGTCTTTGAATTAATTTAACATGATCTCGGTCATTTTAAGAATTTTGAATGGTATTTGAGCACAGAAATGAAT
GTGCATTTCTTATATGTTCAAATTGAGCTTATATTGATTTTATGATCCCTTTGGAGGGTGTCTCTATGTTTTCTAAGTTTTTTCTCTGACATATATTGTTTGCATTCATGAGGATTGCTTTTATTTTACTTGTTAATATTTGAAGAATCAACCTGCACCCCACTCTCTCTTTTTGAAGTAGTACTTCC

>TRINITY_DN38826_c0_g1_i3 from sample A6 / Example unspliced isoform:

TCAAAGTGAGAAAATCACTCCGGAGGGCGGACTTGAAACAGATTCCAAAACAGTATGTCCTTGAATAAACAACCAGGAATGTTATATAAGTCTCAGAGGCCAAAGGTCTTATGAGAGGGGAGGAAGTAAACACCAGGGTTTTGATTTTTGCGCAAGGGTCTGTTTCATATCTGAAGGTGGTGGGAGCGGATTTGCGGCGG
GCAGCTATATTGTGAGCAGCATATAGAGGCGCGATAAAAATGGAAGCCGTGGACGCCGTTGTAAGGCCGTTGCAGGAATTTGCCAAGGATAGCGTGCGCCTTGTCAAAAGATGCCACAAGCCTGATCGCAAAGAGTTTAGTAAAGTGGCTTTCAGGACGGCCATTGGATTTGTTGTTATGGGATTTGTTGGTTTCTTTGT
CAAGTTGATCTTCATTCCAATCAACAACATCATAGTGGGATCAGGATAGgtaagctctttttttaaaaatgattttcaacagttaatggtgaattaaaagcaaaatagatattcttaaaaaatttctatcatgtactgaaaggcactagaattggaaaattgtaagATGAAACAGACTATAATGAAGCAGAAGCAAGAAC
TATGGCTGAAGTGATATCTCACCAACAATTAAGGTGGTTGAAAGAGTCTTTGAATTAATTTAACATGATCTCGGTCATTTTAAGAATTTTGAATGGTATTTGAGCACAGAAATGAATGTGCATTTCTTATATGTTCAAATTGAGCTTATATTGATTTTATGATCCCTTTGGAGGGTGTCTCTATGTTTTCTAAGTTTTTT
CTCTGACATATATTGTTTGCATTCATGAGGATTGCTTTTATTTTACTTGTTAATATTTGAAGAATCAACCTGCACCCCACTCTCTCTTTTTGAAGTAGTACTTCC

Intron 4 GTAAGGTGGA ... GGCACACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.88	178	13.5	31.3
A2	12.79	210.3	24	28.5
A3	12.89	269.9	25.3	35.8
A4	19.36	140.1	18.5	30.8
A5	17.01	180	13.5	35.3
A6	13.85	2760.6	122	413
A7	12.18	1686	97.5	213.3
A8	13.66	1324.4	54.3	194.8
A9	13.32	1706.4	104.6	239.4
A10	11.03	1352	73.6	156.3

>TRINITY_DN114333_c0_g1_i5 from sample A1 / Example spliced isoform

GGAGGAAGTAAACACCAGGGTTTTGATTTTTGCGCAAGGGTCTGTTTCATATCTGAAGGTGGTGGGAGCGGATTTACGGCGGGCAGCTATATCGTGAGCAGCATATAGAGGCGCGATAAAAATGGAAGCCGTGGACGCCGTTGTAAGGCCGTTGCAGGAATTTGCCAAGGATAGCGTGCGCCTTGTCAAAAGATGCCACA
AGCCTGATCGCAAAGAGTTTAGTAAAGTGGCTTTCAGGACGGCCATTGGATTTGTTGTTATGGGATTTGTTGGTTTCTTTGTCAAGTTGATCTTCATTCCAATCAACAACATCATAGTGGGATCAGGATAGGTAAGCTCTTTTTTTAAAAATGATTTTCAACAGTTAATGGTGAATTAAAAGCAAAATAGATATTCTTAA
AAAATTTCTATCATGTACTGAAAGGCACTAGAATTGGAAAATTGTAAGATGAAACAGACTATAATGAAGCAGAAGCAAGAACTATGGCTGAAGTGATATCTCACCAACAATTAAGGTGGTTGAAAGAGTCTTTGAATTAATTTAACATGATCTCGGTCATTTTAAGAATTTTGAATGGTATTTGAGCACAGAAATGAATG
TGTATTTCTTATATGTTCAAATTGAGCTTATATTGATTTTATGATCCCTTTGGAGGGGGTGTCTCTATGTTTTCTAAGTTTTTTCTCTGACATATATTGTTTGCATTCATGAGGATTGCTTTTATTTTACTTGTTAATATTTGAAGAATCAACCTGCACCCCACTCTCTCTTTTTGAAGTAGTACTTCTACATCTTTGCC
TCAATTGTTGGTTTGTGTTTAACATTGAAATAGTTGTTTATTTGAGGTGCATGTATTGGATCAATAATAAAGCTTGCTGTAGGTGACACTAACATTTTGTTGAATAATTCAAATTGAATTTCAGTTTAGTATTTTAAAGGTTATTCTGTCATTTGTTCTGCTTTTTAAATTGAATTTATTTTGATTAGCTGGATGATTCA
CACAGGATTCTGTTAAGGAAAAAATATCTAAAGTTAACTTTGTTATCTAAGGTCTTTGTTTTAACTTTCCCTTTTTAATACCCAGTATCTTCCCAAGGCTTTTTTGCAAGTTGTTGTAAGTTCAACTTTGGAAGTCTTTAAAGCTCTCTATTTGGAGAGGGTTTATGCAAAATGGTCCTAAGTTGGCTGAATTTCAGGTC
CAGTATGGTTTAAACAATTTTTTGTGGGAAGCCCATCTAACAGCTGTTTTGGACAGACCCTATGTTCTGGATTAATAATGCTAGATTCTTAAAATAGATAAATAATAAATTAAAG

>TRINITY_DN114333_c0_g1_i1 from sample A1 / Example unspliced isoform:

GGAGGAAGTAAACACCAGGGTTTTGATTTTTGCGCAAGGGTCTGTTTCATATCTGAAGGTGGTGGGAGCGGATTTGCGGCGGGCAGCTATATTGTGAGCAGCATATAGAGgtaaggtggagctattatgcatcagtatatgtcaatttcaatcagatctgttaagaaagtgagtccaacaattcacagaaagcttgtgtt
ggcacaccagGCGCGATAAAAATGGAAGCCGTGGACGCCGTTGTAAGGCCGTTGCAGGAATTTGCCAAGGATAGCGTGCGCCTTGTCAAAAGATGCCACAAGCCTGATCGCAAAGAGTTTAGTAAAGTGGCTTTCAGGACGGCCATTGGATTTGTTGTTATGGGATTTGTTGGTTTCTTTGTCAAGTTGATCTTCATTCC
AATCAACAACATCATAGTGGGATCAGGATAGG