NA ----> NA

Intron 1 GTACGAAGGA ... ATGCTCAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48	26	22	26
A2	52.94	20	27	18
A3	77.36	6	22	19
A4	68.54	14	30	31
A5	70	6	14	14
A6	71.15	44	118	99
A7	71.1	51	128	123
A8	73.55	48	132	135
A9	75.38	41	120	131
A10	71.96	38	107	88

>TRINITY_DN34190_c0_g2_i2 from sample A7 / Example spliced isoform

CCATGTAAAACCTTTCCCTCCGTTAATGGCAAGGCATTGGCATCAAAGTTCCGTAGCAATATGAAAATACTAGCGTATGTTACAGTAACATATCTTTTTGTCATCATCAAAATTAAGCAAGGCAGGCTTTACCTTTCGGTCGGATGGGTTGGTCGGGCTCGTCCATTGAATTGATCTGACCAGTATTTATTCCAATGAAG
GATGTCACCTTTTTCCTCTACCATTCTTGACAGGAGAATTTCACAGTCGTGGTAAACAGTGCTCAAGTATACAATACAAATCTGCATTTTGCACTTGGGTTGTATAGCGGAGCAGGAAGCAGCGCAAATCTTGCTGGATCGTGGACAAATTCCCCCCTAAGCAATGGCAAATGAATCAAAGAAGGAAGCTTTTAGGAAAT
ACTTGGAGTCAAGTGGTGTGCTTGATGCCCTCACTAAAGTTCTTGTTGCTTTATATGAGGAGAATGAGAAGCCCTCTGTAGCAATTGAATTCATTCAACGCAGCTTGGGCGGACCCACTTCATCTGAATATGAAAAACTTCAAGCGGAGAAAGCAGAACTGCAATCCAATTGGAGGAATTACGGAATCCATCAACCCATC
GAGAGCAAGAAAATGATGAGCCAATAGCTAGCAAAGATTCGTAACTTCAAAAACTTTTGTGGAGGATTCAATACTTACAATATCAGTTTGTAGGTTAGGAAATGTACAATAGTGACAGCTTACCGTGTTTGGTTAGATGTGTGATCTCCCTCTCACCTTGCTGACACCCCCGTCTACTGCATTTAGAGACAAATGTGATC
TTCCTCCCCCCTTGGCCGATGCCTCATCTGTTTGGATGTGGATGGCAGTATTACATTTCCATTTATTGAGACAAGGGATTAATGTGTCTCTTCATCTCCAAGAACTGAACATTGTCGCTAGCCTGGGAACAGTAACGTTTCAACACTTTCATAGAATTCATTGACAATTGCAATAGCTATTCTATTCAATGTTAGCGTGT
TTAATGGCTTACTGTGTTCCTTTTGGAAGGAAATCCTTGTTCAAGCAAACTGTTGTGATTTTTTGTTTTTTCTTGTCTGAATCATGCACGAGAAAACTTATCCTAATATTTTGAATTCCTCTAGATTTGCAATACGTGGTTTAACTGAAATGATTGAGCAGACACATGAAACAGTGAATCTATGATGGAAAGTAAAATTT
GTAAAGACCTCTTGTAATTTATATATATTCCATGAACATGTGATGCTGATGCTCAATTGTGTTGCCTT

>TRINITY_DN34190_c0_g2_i1 from sample A7 / Example unspliced isoform:

CCATGTAAAACCTTTCCCTCCGTTAATGGCAAGGCATTGGCATCAAAGTTCCGTAGCAATATGAAAATACTAGCGTATGTTACAGTAACATATCTTTTTGTCATCATCAAAATTAAGCAAGGCAGGCTTTACCTTTCGGTCGGATGGGTTGGTCGGGCTCGTCCATTGAATTGATCTGACCAGTATTTATTCCAATGAAG
GATGTCACCTTTTTCCTCTACCATTCTTGACAGGAGAATTTCACAGTCGTGGTAAACAGTGCTCAAGTATACAATACAAATCTGCATTTTGCACTTGGGTTGTATAGCGGAGCAGGAAGCAGCGCAAATCTTGCTGGATCGTGGACAAATTCCCCCCTAAGCAATGGCAAATGAATCAAAGAAGGAAGCTTTTAGGAAAT
ACTTGGAGTCAAGTGGTGTGCTTGATGCCCTCACTAAAGTTCTTGTTGCTTTATATGAGGAGAATGAGAAGCCCTCTGTAGCAATTGAATTCATTCAACGCAGCTTGGGCGGACCCACTTCATCTGAATATGAAAAACTTCAAGCGGAGAAAGCAGAACTGCAATCCAAgtacgaaggattgcttgcatctcatgaaaag
gcatgctcagagTTGGAGGAATTACGGAATCCATCAACCCATCGAGAGCAAGAAAATGATGAGCCAATAGCTAGCAAAGATTCGTAACTTCAAAAACTTTTGTGGAGGATTCAATACTTACAATATCAGTTTGTAGGTTAGGAAATGTACAATAGTGACAGCTTACCGTGTTTGGTTAGATGTGTGATCTCCCTCTCACC
TTGCTGACACCCCCGTCTACTGCATTTAGAGACAAATGTGATCTTCCTCCCCCCTTGGCCGATGCCTCATCTGTTTGGATGTGGATGGCAGTATTACATTTCCATTTATTGAGACAAGGGATTAATGTGTCTCTTCATCTCCAAGAACTGAACATTGTCGCTAGCCTGGGAACAGTAACGTTTCAACACTTTCATAGAAT
TCATTGACAATTGCAATAGCTATTCTATTCAATGTTAGCGTGTTTAATGGCTTACTGTGTTCCTTTTGGAAGGAAATCCTTGTTCAAGCAAACTGTTGTGATTTTTTGTTTTTTCTTGTCTGAATCATGCACGAGAAAACTTATCCTAATATTTTGAATTCCTCTAGATTTGCAATACGTGGTTTAACTGAAATGATTGA
GCAGACACATGAAACAGTGAATCTATGATGGAAAGTAAAATTTGTAAAGACCTCTTGTAATTTATATATATTCCATGAACATGTGATGCTGATGCTCAATTGTGTTGCCTT

Intron 2 GTATGTTACA ... TTCTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	81.25	27	5	117
A2	85.86	14	15	85
A3	86.76	9	11	59
A4	80.49	24	15	99
A5	86.11	5	7	31
A6	84.06	33	27	174
A7	87.87	41	23	297
A8	91.61	13	23	142
A9	81.97	55	53	250
A10	86.98	41	24	274

>TRINITY_DN38876_c0_g1_i2 from sample A6 / Example spliced isoform

TAATGCATCACCATGTAAAACCTTTCCCTCCGTTAATGGCAAGGCATTGGCATCAAAGTTCCGTAGCAATATGAAAATACTAGCGAGAATTTCACAGTCGTGGTAAACAGTGCTCAAGTATACAATACAAATCTGCATTTTGCACTTGGGTTGTATAGCGGAGCAGGAAGCAGCGCAAATCTTGCTGGATCGTGGACAAA
TTCCCCCCTAAGCAATGGCAAATGAATCAAAGAAGGAAGCTTTTAGGAAATACTTGGAGTCAAGTGGTGTGCTTGATGCCCTCACTAAAGTTCTTGTTGCTTTATATGAGGAGAATGAGAAGCCCTCTGTAGCAATTGAATTCATTCAACGCAGCTTGGGCGGACCCACTTCATCTGAATATGAAAAACTTCAAGCGGAG
AAAGCAGAACTGCAATCCAAGTACGAAGGATTGCTTGCATCTCATGAAAAGGCATGCTCAGAGTTGGAGGAATTACGGAATCCATCAACCCATCGAGAGCAAGAAAATGATGAGCCAATAGCTAGCAAAGATTCGTAACTTCAAAAACTTTTGTGGAGGATTCAATACTTACAATATCAGTTTGTAGGTTAGGAAATGTA
CAATAGTGACAGCTTACCGTGTTTGGTTAGATGTGTGATCTCCCTCTCACCTTGCTGACACCCCCGTCTACTGCATTTAGAGACAAATGTGATCTTCCTCCCCCCTTGGCTGATGCCTCATCTGTTTGGATGTGGATGGCAGTATTACATTTCCATTTATTGAGACAAGGGATTAATGTGTCTCTTCATCTCCAAGAACT
GAACATTGTCGCTAGCCTGGGAACAGTAACGTTTCAACACTTTCATAGAATTCATTGACAATTGCAATAGCTATTCTATTCAATGTTAGCGTGTTTAATGGCTTACTGTGTTCCTTTTGGAAGGAAATCCTTGTTCAAGCAAACTGTTGTGATTTTTTGTTTTTTCTTGTCTGAATCATGCACGAGAAAACTTATCCTAA
TATTTTGAATTCCTCTAGATTTGCAATACGTGGTTTAACTGAAATGATTGAGCAGACACATGAAACAGTGAATCTATGATGGAAAGTAAAATTTGTAAAGACCTCTTGTAATTTATATATATTCCATGA

>TRINITY_DN38876_c0_g1_i4 from sample A6 / Example unspliced isoform:

TAATGCATCACCATGTAAAACCTTTCCCTCCGTTAATGGCAAGGCATTGGCATCAAAGTTCCGTAGCAATATGAAAATACTAGCgtatgttacagtaacatatctttttgtcatcatcaaaattaagcaaggcaggctttacctttcggtcggatgggttggtcgggctcgtccattgaattgatctgaccagtatttat
tccaatgaaggatgtcacctttttcctctaccattcttgacagGAGAATTTCACAGTCGTGGTAAACAGTGCTCAAGTATACAATACAAATCTGCATTTTGCACTTGGGTTGTATAGCGGAGCAGGAAGCAGCGCAAATCTTGCTGGATCGTGGACAAATTCCCCCCTAAGCAATGGCAAATGAATCAAAGAAGGAAGCT
TTTAGGAAATACTTGGAGTCAAGTGGTGTGCTTGATGCCCTCACTAAAGTTCTTGTTGCTTTATATGAGGAGAATGAGAAGCCCTCTGTAGCAATTGAATTCATTCAACGCAGCTTGGGCGGACCCACTTCATCTGAATATGAAAAACTTCAAGCGGAGAAAGCAGAACTGCAATCCAAGTACGAAGGATTGCTTGCATC
TCATGAAAAGGCATGCTCAGAGTTGGAGGAATTACGGAATCCATCAACCCATCGAGAGCAAGAAAATGATGAGCCAATAGCTAGCAAAGATTCGTAACTTCAAAAACTTTTGTGGAGGATTCAATACTTACAATATCAGTTTGTAGGTTAGGAAATGTACAATAGTGACAGCTTACCGTGTTTGGTTAGATGTGTGATCT
CCCTCTCACCTTGCTGACACCCCCGTCTACTGCATTTAGAGACAAATGTGATCTTCCTCCCCCCTTGGCTGATGCCTCATCTGTTTGGATGTGGATGGCAGTATTACATTTCCATTTATTGAGACAAGGGATTAATGTGTCTCTTCATCTCCAAGAACTGAACATTGTCGCTAGCCTGGGAACAGTAACGTTTCAACACT
TTCATAGAATTCATTGACAATTGCAATAGCTATTCTATTCAATGTTAGCGTGTTTAATGGCTTACTGTGTTCCTTTTGGAAGGAAATCCTTGTTCAAGCAAACTGTTGTGATTTTTTGTTTTTTCTTGTCTGAATCATGCACGAGAAAACTTATCCTAATATTTTGAATTCCTCTAGATTTGCAATACGTGGTTTAACTG
AAATGATTGAGCAGACACATGAAACAGTGAATCTATGATGGAAAGTAAAATTTGTAAAGACCTCTTGTAATTTATATATATTCCATGA