sp|P51423|RL40_BRARP ----> Ubiquitin-ribosomal protein eL40 fusion protein

Intron 1 GTAGGCTGAT ... CTTATTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.11	127	26	34
A2	15.54	125	25	21
A3	21.25	139	38	37
A4	20.75	63	15	18
A5	13.3	101	13	18
A6	15.58	428	71	87
A7	17.27	690	133	155
A8	18.39	587.9	103.9	161
A9	16.35	637	128	121
A10	16.76	606	107	137

>TRINITY_DN128441_c3_g3_i2 from sample A1 / Example spliced isoform

GCAGATCTTTGTCAAAACCCTCACCGGGAAGACCATCCTTTTGAATTGTAGCTTGTGTTATCATATTGCACAATTGGAGAGCAATTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTACATCCAAAAGGCAAGAATCAGCATAGAGCACTCTTAAGGCCAAGGAAATCGGGCACAAAGTCA
TGTTACAGTACTTTGTATCCACAAGAACTTGGGGAGGCTGTAGTTGACAGACTTGATGCACTCAACGACCGAGGGCAGGAGCTTGGGAACCGGATAAGGGCAGTGGAGGAGCGGTTGTCTGCAATGGAACATGTGATAGATAGGCAGTGGCAGACAATCCAGTTGCTCCAGGAGCAACTAGCGGCCCAGAGGGTAGCGTT
GCGTGAGAATGGGATAGTGGCAGTGGAGGAACAGTTGTCTGCAATGGAAGAGGTAATAGAAAGGCAGAGACAGACAATCCAGTCGCTGCAACAGCAACTAGCAGCACAGAGGGCAGCATTTGACCAACAAATTCTGGTTTTGGCAAATCAGATCTGTGGCTTGAGGATAAGACGGAACCCCCCTTCAGCAATGGATAATA
TGGAACCCAAAGATGAAAACATGGAAGGTGATTCAAGCTAAGCAGAGAACGAAGGTGACTCCATCAGTGCTTTAAAAAACACAGTACGGTAGCAATTTTGGCTAAACAAAAGGTATGTTTCTTTGCCTATATGCTGGTCTAGTGGAGGACAATATGTCCGTATATATTTTAGCAGCAGAATGCAGGAAAATAGGAATAGC
TATAATGCCTTAATTATGCTGGAAAAGACTAAGACTCTGAATTTTGTATTTGGTCGGATTATCTGGTCATTTTGTACCAACCTGCCTCCAAGTTATGCTGAGGCTCTCTTTTTAATACAATACGAAGAAACTTCTTTCGAGTTAAACAAGATACAATGGAACAAGCCTTCAAAGTGTATTGCTGTACACATTGTTGAAAA
CTGGATGGGCCAGAGTTTGTACCAATAATTGTCCTCCGTCGAGGTATATCTCTTTAAATGGTTTGATCTAAAATCTGCATTGTTGTCCACACACGTCTACGATTGACAACCTTGTCTTTGCTTTAAAAATTAAATGTTTTTTAATGTCAATATTAAACTTCCAAGGGAGAAACCACATAATCCCTGAAGGGGGCACTTCA
TAAATGTTTCTATTATCTAAAATATTACAAGTGGTTATTCCAGGGAGAAACTACATAATCCCTAAAGGGGGCACTTCATAAATGTTT

>TRINITY_DN128441_c3_g3_i4 from sample A1 / Example unspliced isoform:

GCAGATCTTTGTCAAAACCCTCACCGGGAAGACCATCCTTTTGAATTGTAGCTTGTGTTATCATATTGCACAATTGGAGAGCAAGTTATGACTCCCTAATTATTGGCCTTTGTTCATGTTGTCTTTTGTTGCTGCAGTTTTTGCTTTTTTGTAAAAACTGCGCTCATGCAAATATTTCCTATAATCATATTTTTTGTGGG
CAAGTATGGTTGCCTTCTCTCATGCCTTTTTTAATTTTGTTGCATGTAGTTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTACATCCAAAAGGCAAGAATCAGCATAGAGCACTCTTAAGGCCAAGGAAATCGGGCACAAAGTCATGTTACAGTACTTTGTATCCACAAGgtaggctgat
atcaatttcatcctatggatgcttgactttatatgcagagaagttgaagatgatttgacttggtttgaattttaaatagcattcagagttaactttatatgcacgctgtttatgatttgtggaggttattgaatttagaggagaggggtatcattcggggataatcagattgcaaagcaggtttcgttttatctgtttaa
tggcaaaactggagttagatcaagttatagactaaatttgttaacattaaaacaatgaatgctgtttgttaatggtacaatgggatcaaaataatgaatacaagaaatatattcagtgtatgcatgcgttatagtcttttaattatgtgccctgttgctttgtattgcaactaaggctttagattgaatagccacttatt
gtgttttgttcttatttttctaactatgctagaatacttaacaaactgagaaactaaatagctgttgaaactcttattgaagAACTTGGGGAGGCTGTAGTTGACAGACTTGATGCACTCAACGACCGAGGGCAGGAGCTTGGGAACCGGATAAGGGCAGTGGAGGAGCGGTTGTCTGCAATGGAACATGTGATAGATAG
GCAGTGGCAGACAATCCAGTTGCTCCAGGAGCAACTAGCGGCCCAGAGGGTAGCGTTGCGTGAGAATGGGATAGTGGCAGTGGAGGAACAGTTGTCTGCAATGGAAGAGGTAATAGAAAGGCAGAGACAGACAATCCAGTCGCTGCAACAGCAACTAGCAGCACAGAGGGCAGCATTTGACCAACAAATTCTGGTTTTGG
CAAATCAGATCTGTGGCTTGAGGATAAGACGGAACCCCCCTTCAGCAATGGATAATATGGAACCCAAAGATGAAAACATGGAAGGTGATTCAAGCTAAGCAGAGAACGAAGGTGACTCCATCAGTGCTTTAAAAAACACAGTACGGTAGCAATTTTGGCTAAACAAAAGGTATGTTTCTTTGCCTATATGCTGGTCTAGT
GGAGGACAATATGTCCGTATATATTTTAGCAGCAGAATGCAGGAAAATAGGAATAGCTATAATGCCTTAATTATGCTGGAAAAGACTAAGACTCTGAATTTTGTATTTGGTCGGATTATCTGGTCATTTTGTACCAACCTGCCTCCAAGTTATGCTGAGGCTCTCTTTTTAATACAATACGAAGAAACTTCTTTCGAGTT
AAACAAGATACAATGGAACAAGCCTTCAAAGTGTATTGCTGTACACATTGTTGAAAACTGGATGGGCCAGAGTTTGTACCAATAATTGTCCTCCGTCGAGGTATATCTCTTTAAATGGTTTGATCTAAAATCTGCATTGTTGTCCACACACGTCTACGATTGACAACCTTGTCTTTGCTTTAAAAATTAAATGTTTTTTA
ATGTCAATATTAAACTTCCAAGGGAGAAACCACATAATCCCTGAAGGGGGCACTTCATAAATGTTTCTATTATCTAAAATATTACAAGTGGTTATTCCAGGGAGAAACTACATAATCCCTAAAGGGGGCACTTCATAAATGTTT

Intron 2 GCATCCCACC ... ACCCTGCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.75	930	764	364
A2	38.58	640	513	291
A3	43.17	657	755	243
A4	40.48	641	650	222
A5	42.93	341	373	140
A6	35.17	5209	3084	2567
A7	38.13	4032	2866	2103
A8	31.76	4096	1652	2160
A9	34.58	4177	2317	2099
A10	38.35	3134	2282	1617

>TRINITY_DN109306_c0_g1_i8 from sample A4 / Example spliced isoform

TTCTCTCGCGCCTCTTCCCACTGAGGCAAGCTGAGAGCTCTGCAATTTTGCAGGACTTGTTCCGTGGCTCTAGTGAAGGCCCAAAGATGCAGATCTTTGTCAAAACTCTCACTGGCAAGACCATCACTCTCGAGGTCGAGAGTTCTGATACAAGGGATTCCACCTGACCAGCAGCGTCTGATCTTTGCTGGAAAGCAGCT
GGAAGACGGCCGAACCCTTGCTGATTACAATATATTGACAATGTCAAGGCCAAGATCCAGGACAAGGAAGGAATTCCCCCAGATCAACAGAGGCTAATTTTTGCTGGTAAGCAGCTGGAGGACGGAAGGACCTTGGCTGACTACAACATTCAGAAGGAATCCACTTTGCATCTTGTGCTTCGTCTGAGAGGTGGAATGCA
AATCTTTGTGAAAACCCTGACTGGGAAAACTATTACACTGGAGGTTGAGAGCTCAGACACCATTGATAATGTGAAGGCCAAGATCCAGGACAAGGAAGGGATTCCACCTGACCAGCAGCGTCTGATCTTTGCTGGAAAGCAGCTGGAAGATGGCCGAACCCTTGCGGACTACAACATCCAAAAGGAATCCACCCTACACC
TCGTTCTCCGTCTGAGAGTCCCCCTGACCAGCAACGTCTGATCTTCGCCGGAAAGCAGCTCGAGGACGGGCGAACCCTTGCCGATTACAATATCCAGAAGGAATCCACCCTCCACCTTGTTCTCCGTCTGAGAGGTGGAATGCAGATCTTTGTTAAAACTCTCACTGGAAAGACAATTACATTGGAAGTTGAGAGCTCGG
ACACCATTGACAACGTGAAGGCCAAGATCCAGGACAAGGAAGGAATTCCCCCAGATCAACAGAGGCTAATTTTTGCTGGTAAGCAGCTGGAGGACGGAAGGACCTTGGCTGACTACAACATTTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTACATCCAAAAGGCAAGAATCAGCATAG
AGCACTCTTAAGGCCAAGGAAATCGGGCACAAAGTCATGTTACAGTACTTTGTATCCACAAGTAACTTGGGGAGGCTGTAGTTGACAGACTTGATGCACTCAACGACCGAGGGCAGGAGCTTGGGAACCGGATAAGGGCAGTGGAGGAGCGGTTGTCTGCAATGGAACATGTGATAGATAGGCAGTGGCAGACAATCCAG
TTGCTCCAGGAGCAACTAGCGGCCCAGAGGGTAGCGTTGCGTGAGAATGGGATAGTGGCAGTGGAGGAACAGTTGTCTGCAATGGAAGAGGTAATAGAAAGGCAGAGACAGACAATCCAGTCGCTGCAACAGCAACTAGCAGCACAGAGGGCAGCATTTGACCAACAAATTCTGGTTTTGGCAAATCAGATCTGTGGCTT
GAGGATAAGACGGAACCCCCCTTCAGCAATGGATAATATGGAACCCAAAGATGAAAACATGGAAGGTGATTCAAGCTAAGCAGAGAACGAAGGTGACTCCATCAGTGCTTTAAAAAACACAGTACGGTAGCAATTTTGGCTAAACAAAAGGTATGTTTCTTTGCCTATATGCTGGTCTAGTGGAGGACAATATGTCCGTA
TATATTTTAGCAGCAGAATGCAGGAAAATAGGAATAGCTATAATGCCTTAATTATGCTGGAAAAGACTAAGACTCTGAATTTTGTATTTGGTCGGATTATCTGGTCATTTTGTACCAACCTGCCTCCAAGTTATGCTGAGGCTCTCTTTTTAATACAATACGAAGAAACTTCTTTCGAGTTAAACAAGATACAATGGAAC
AAGCCTTCAAAGTGTATTGCTGTACACATTGTTGAAAACTGGATGGGCCAGAGTTTGTACCAATAATTGTCCTCCGTCGAGGTATATCTCTTTAAATGGTTTGATCTAAAATCTGCATTGTTGTCCACACACGTCTACGATTGACAACCTTGTCTTTGCTTTAAAAATTAAATGTTTTTTAATGTCAATATTAAACTTCC
AAGGGAGAAACCACATAATCCCTGAAGGGGGCACTTCATAAATGTTTCTATTATCTAAAATATTACAAGTGGTTATTCCAGGGAGAAACTACATAATCCCTAAAGGGGGCACTTCATAAATGTTTCTATTATCTAAAATATTACAAGTGGTTATTCCATATCGATAGATAACAGAAGATATCTTCTCGATTATTTGGACA
TACTACGAGCACTACATACCATCCAAAGTTCAAACTAAATCATCAAAAATAGAAAACATATTGCATAGTTGACACTCCTCATGAGCCATAAGGTAGATATTGTGCATTAAAACTCGGAATTTGGTTTATCATTTAAAATATTCCAAGTGATTATGGAAATATTGCAGGTGATTATCGTTTTGCAGTCTCAATGAGTTAAA
AAATAGAAAACATATTGCACAGTTGACACTCATGAGCCACAAGGTAAAAGGTAGATAGTGGTGTGCATTATAACTAGGAATTTGAGTTGAGTTCTATCATATAAAATATTACACAAGTGTTTATTCCATATAGATATCTTCTCAATTATATATATAGCAAAAAATATCTTCTCGATTATTCTAGCATGAGTAGTATATAT
ATCATCCAAACTAAATCATATTAAATAAATAACATATCGTGAAATTGACATTCATAAATCATAAAAAATAGAAAACACATTGCAGAGCTGTCATTCCTTATTATAGAAAACACATTGCACAGTTGTCATTCATTAATTGATTGTAGATAGTGATGCACTAAAGCAAATTCTATCATCTAAAACATTAAAATATTGCAAGT
GATTAATAACATAAAAAAATAGAAAACATATCACACAATCGAGACTCATGAATCATGATCTAGATAGGGGTGTATTAAAGCTTAAGCATTTTGTTCAACTTCTATCATCTAAAATACTCATAAAGCTAAGCAGTTAGTTTAACTATCATTTAAAATTCAAAGTGAAGCAGAAGGAATCCACT

>TRINITY_DN109306_c0_g1_i1 from sample A4 / Example unspliced isoform:

CCGTCTGAGGGGTGGCATGCAGATCTTTGTTAAAACCCTCACTGGCAAGACGATTACACTAGAAGTTGAGAGCTCGGACACCATTGATAATGTCAAAGCCAAGATTCAGGACAAGGAAGGCATCCCCCCTGATCAGCAGCGTCTGATCTTTGCTGGGAAGCAGCTCGAAGACGGCCGAACCCTTGCTGATTACAATATCC
AGAAGGAATCCACCCTGCATCTTGTCCTCCGGTTGAGAGGAGGCATGCAGATCTTTGTCAAAACTCTTACCGGGAAGACAATTACTTTGGAAGTTGAGAGTTCAGATACCATTGACAATGTGAAGGCCAAGATCCAGGACAAGGAAGGGATTCCACCTGACCAGCAGCGTCTGATCTTTGCTGGAAAGCAGCTGGAAGAC
GGCCGAACCCTTGCTGATTACAATATCCAGAAGGAATCCACCCTGCATCTTGTCCTACGTCTGAGAGGTGGTATGCAGATCTTCGTCAAAACCCTTACCGGTAAGACCATCACTCTGGAAGTTGAGAGCTCAGACACCATCGACAATGTCAAGGCAAAGATCCAGGACAAGGAAGgcatcccacccgaccaacagcgact
gatcttcgctggaaagcagctcgaagatggccgaacccttgctgactacaatattcagaaggaatccaccctgcacctcgtactccgtctgagaggtggcatgcaaatgtcaaggcaaagatccaggacaaggaaggcatcccacccgaccaacagcgactgatcttcgctggaaagcagctcgaagatggccgaaccct
tgctgactacaatattcagaaggaatccaccctgcaagGAATTCCCCCAGATCAACAGAGGCTAATTTTTGCTGGTAAGCAGCTGGAGGACGGAAGGACCTTGGCTGACTACAACATTCAGAAGGAATCCACTTTGCATCTTGTGCTTCGTCTGAGAGGTGGAATGCAAATCTTTGTGAAAACCCTGACTGGGAAAACTA
TTACACTGGAGGTTGAGAGCTCAGACACCATTGATAATGTGAAGGCCAAGATCCAGGACAAGGAAGGGATTCCACCTGACCAGCAGCGTCTGATCTTTGCTGGAAAGCAGCTGGAAGATGGCCGAACCCTTGCGGACTACAACATCCAAAAGGAATCCACCCTACACCTCGTTCTCCGTCTGAGAGTCCCCCTGACCAGC
AACGTCTGATCTTCGCCGGAAAGCAGCTCGAGGACGGGCGAACCCTTGCCGATTACAATATCCAGAAGGAATCCACCCTCCACCTTGTTCTCCGTCTGAGAGGTGGAATGCAGATCTTTGTTAAAACTCTCACTGGAAAGACAATTACATTGGAAGTTGAGAGCTCGGACACCATTGACAACGTGAAGGCCAAGATCCAG
GACAAGGAAGGAATTCCCCCAGATCAACAGAGGCTAATTTTTGCTGGTAAGCAGCTGGAGGACGGAAGGACCTTGGCTGACTACAACATTCAGAAGGAATCCACTTGGAGGTTGAGAGCTCAGACACCATTGATAATGTGAAGGCCAAGATCCAGGACAAGGAAGGGAT

Intron 3 GTGGCATGCA ... TTGCATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70.28	140	331	38
A2	68.46	117	254	16
A3	66.31	156	307	29
A4	65.67	126	241	14
A5	64.17	91	163	18
A6	82.03	216	986	51
A7	68.87	401	887	98
A8	66.3	304	598	91
A9	68.55	434	946	119
A10	68.54	374	815	78

>TRINITY_DN121866_c0_g1_i2 from sample A2 / Example spliced isoform

TGCAGATCTTTGTCAAAACTCTCACTGGCAAGACCATCACTCTCGAGGTCGAGAGTTCTGATACAATTGACAATGTCAAGGCCATGATCCAGGACAAGGAAGGGATTCCACCTGACCAGCAGCGTCTGATCTTTGCTGGAAAGCAGCTGGAAGATGGCCGAACCCTTGCGGACTACAACATCCAAAAGGAATCCACCCTA
CACCTCGTTCTCCGTCTGAGAGTTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTACATCCAAAAGGCAAGAATCAGCATAGAGCACTCTTAAGGCCAAGGAAATCGGGCACAAAGTCATGTTACAGTACTTTGTATCCACAAGAACTTGGGGAGGCTGTAGTTGACAGACTTGATGCACT
CAACGACCGAGGGCAGGAGCTTGGGAACCGGATAAGGGCAGTGGAGGAGCGGTTGTCTGCAATGGAACATGTGATAGATAGGCAGTGGCAGACAATCCAGTTGCTCCAGGAGCAACTAGCGGCCCAGAGGGTAGCGTTGCGTGAGAATGGGATAGTGGCAGTGGAGGAACAGTTGTCTGCAATGGAAGAGGTAATAGAAA
GGCAGAGACAGACAATCCAGTCGCTGCAACAGCAACTAGCAGCACAGAGGGCAGCATTTGACCAACAAATTCTGGTTTTGGCAAATCAGATCTGTGGCTTGAGGATAAGACGGAACCCCCCTTCAGCAATGGATAATATGGAACCCAAAGATGAAAACATGGAAGGTGATTCAAGCTAAGCAGAGAACGAAGGTGACTCC
ATCAGTGCTTTAAAAAACACAGTACGGTAGCAATTTTGGCTAAACAAAAGGTATGTTTCTTTGCCTATATGCTGGTCTAGTGGAGGACAATATGTCCGTATATATTTTAGCAGCAGAATGCAGGAAAATAGGAATAGCTATAATGCCTTAATTATGCTGGAAAAGACTAAGACTCTGAATTTTGTATTTGGTCGGATTAT
CTGGTCATTTTGTACCAACCTGCCTCCAAGTTATGCTGAGGCTCTCTTTTTAATACAATACGAAGAAACTTCTTTCGAGTTAAACAAGATACAATGGAACAAGCCTTCAAAGTGTATTGCTGTACACATTGTTGAAAACTGGATGGGCCAGAGTTTGTACCAATAATTGTCCTCCGTCGAGGTATATCTCTTTAAATGGT
TTGATCTAAAATCTGCATTGTTGTCCACACACGTCTACGATTGACAACCTTGTCTTTGCTTTAAAAATTAAATGTTTTTTAATGTCAATATTAAACTTCCAAGGGAGAAACCACATAATCCCTGAAGGGGGCACTTCATAAATGTTTCTATTATCTAAAATATTACAAGTGGTTATTCCAGGGAGAAACTACATAATCCC
TAAAGGGGGCACTTCATAAATGTTT

>TRINITY_DN121866_c0_g1_i1 from sample A2 / Example unspliced isoform:

TGCAGATCTTTGTCAAAACTCTCACTGGCAAGACCATCACTCTCGAGGTCGAGAGTTCTGATACAATTGACAATGTCAAGGCCATGATCCAGGACAAGGAAGGGATTCCACCTGACCAGCAGCGTCTGATCTTTGCTGGAAAGCAGCTGGAAGATGGCCGAACCCTTGCGGACTACAACATCCAAAAGGAATCCACCCTA
CACCTCGTTCTCCGTCTGAGAGgtggcatgcagatctttgtcaaaaccctcaccgggaagaccatccttttgaattgtagcttgtgttatcatattgcacaattggagagcaagttatgactccctaattattggcctttgttcatgttgtcttttgttgctgcagtttttgcttttttgtaaaaactgcgctcatgcaa
atatttcctataatcatattttttgtgggcaagtatggttgccttctctcatgcctttttttaattttgttgcatgtagTTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTACATCCAAAAGGCAAGAATCAGCATAGAGCACTCTTAAGGCCAAGGAAATCGGGCACAAAGTCATGTTA
CAGTACTTTGTATCCACAAGAACTTGGGGAGGCTGTAGTTGACAGACTTGATGCACTCAACGACCGAGGGCAGGAGCTTGGGAACCGGATAAGGGCAGTGGAGGAGCGGTTGTCTGCAATGGAACATGTGATAGATAGGCAGTGGCAGACAATCCAGTTGCTCCAGGAGCAACTAGCGGCCCAGAGGGTAGCGTTGCGTG
AGAATGGGATAGTGGCAGTGGAGGAACAGTTGTCTGCAATGGAAGAGGTAATAGAAAGGCAGAGACAGACAATCCAGTCGCTGCAACAGCAACTAGCAGCACAGAGGGCAGCATTTGACCAACAAATTCTGGTTTTGGCAAATCAGATCTGTGGCTTGAGGATAAGACGGAACCCCCCTTCAGCAATGGATAATATGGAA
CCCAAAGATGAAAACATGGAAGGTGATTCAAGCTAAGCAGAGAACGAAGGTGACTCCATCAGTGCTTTAAAAAACACAGTACGGTAGCAATTTTGGCTAAACAAAAGGTATGTTTCTTTGCCTATATGCTGGTCTAGTGGAGGACAATATGTCCGTATATATTTTAGCAGCAGAATGCAGGAAAATAGGAATAGCTATAA
TGCCTTAATTATGCTGGAAAAGACTAAGACTCTGAATTTTGTATTTGGTCGGATTATCTGGTCATTTTGTACCAACCTGCCTCCAAGTTATGCTGAGGCTCTCTTTTTAATACAATACGAAGAAACTTCTTTCGAGTTAAACAAGATACAATGGAACAAGCCTTCAAAGTGTATTGCTGTACACATTGTTGAAAACTGGA
TGGGCCAGAGTTTGTACCAATAATTGTCCTCCGTCGAGGTATATCTCTTTAAATGGTTTGATCTAAAATCTGCATTGTTGTCCACACACGTCTACGATTGACAACCTTGTCTTTGCTTTAAAAATTAAATGTTTTTTAATGTCAATATTAAACTTCCAAGGGAGAAACCACATAATCCCTGAAGGGGGCACTTCATAAAT
GTTTCTATTATCTAAAATATTACAAGTGGTTATTCCAGGGAGAAACTACATAATCCCTAAAGGGGGCACTTCATAAATGTTT

Intron 4 GTGGCATGCA ... TTGCATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.18	121.8	228.6	38
A2	14.16	99.9	159.3	16
A3	18.87	129.7	187.2	29
A4	11.65	109	142.2	14
A5	20.4	74.8	102	18
A6	22.16	187.5	561.7	51
A7	22.72	347	518.3	99
A8	26.42	261.1	356.1	91
A9	25.33	369.8	559.5	121
A10	20.51	316	492.5	78

>TRINITY_DN121624_c1_g2_i2 from sample A3 / Example spliced isoform

GTTGTGCGACTCTTTTCTGAGAGCTTTTCATCCGAAGGTATATCCCTAGTTATTTTCCGGTCTCAAGATGCAGATCTTTGTCAAAACCCTCACAGGCAAGACCATTACTCTCGAGGTCGAGAGCTCTGACACCATTGATAATGTTAAGGCTAAGATCCAGGACAAGGAAGGGATTCCACCTGACCAGCAGCGTCTGATCT
TTGCTGGAAAGCAGCTGGAAGATGGCCGAACCCTTGCGGACTACAACATCCAAAAGGAATCCACCCTACACCTCGTTCTCCGTCTGAGAGTTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTACATCCAAAAGGCAAGAATCAGCATAGAGCACTCTTAAGGCCAAGGAAATCGGGCACA
AAGTCATGTTACAGTACTTTGTATCCACAAGGTAGGCTGATATCAATTTCATCCTATGGATGCTTGACTTTATATGCAGAGAAGTTGAAGATGATTTGACTTGGTTTGAATTTTAAATAGCATTCAGAGTTAACTTTATATGCAC

>TRINITY_DN121624_c1_g2_i3 from sample A3 / Example unspliced isoform:

GTTGTGCGACTCTTTTCTGAGAGCTTTTCATCCGAAGGTATATCCCTAGTTATTTTCCGGTCTCAAGATGCAGATCTTTGTCAAAACCCTCACAGGCAAGACCATTACTCTCGAGGTCGAGAGCTCTGACACCATTGATAATGTTAAGGCTAAGATCCAGGACAAGGAAGGGATTCCACCTGACCAGCAGCGTCTGATCT
TTGCTGGAAAGCAGCTGGAAGATGGCCGAACCCTTGCGGACTACAACATCCAAAAGGAATCCACCCTACACCTCGTTCTCCGTCTGAGAGgtggcatgcagatctttgtcaaaaccctcaccgggaagaccatccttttgaattgtagcttgtgttatcatattgcacaattggagagcaagttatgactccctaattat
tggcctttgttcatgttgtcttttgttgctgcagtttttgcttttttgtaaaaactgcgctcatgcaattatttcctataatcatattttttgtgggcaagtatggttgccttctctcatgccttttttaattttgttgcatgtagTTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTAC
ATCCAAAAGGCAAGAATCAGCATAGAGCACTCTTAAGGCCAAGGAAATCGGGCACAAAGTCATGTTACAGTACTTTGTATCCACAAGGTAGGCTGATATCAATTTCATCCTATGGATGCTTGACTTTATATGCAGAGAAGTTGAAGATGATTTGACTTGGTTTGAATTTTAAATAGCATTCAGAGTTAACTTTATATGCA
C

Intron 5 GTTATGACTC ... TTGCATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.6	78	56	38
A2	25.62	45	15	16
A3	27.12	86	35	29
A4	25.71	39	13	14
A5	29.31	41	16	18
A6	31.78	117	58	51
A7	31.48	210	95	98
A8	31.26	188	80	91
A9	29.24	271	105	119
A10	28.76	192	77	78

>TRINITY_DN121866_c0_g1_i5 from sample A2 / Example spliced isoform

TGCAGATCTTTGTCAAAACTCTCACTGGCAAGACCATCACTCTCGAGGTCGAGAGTTCTGATACAATTGACAATGTCAAGGCCATGATCCAGGACAAGGAAGGGATTCCACCTGACCAGCAGCGTCTGATCTTTGCTGGAAAGCAGCTGGAAGATGGCCGAACCCTTGCGGACTACAACATCCAAAAGGAATCCACCCTA
CACCTCGTTCTCCGTCTGAGAGGTGGCATGCAGATCTTTGTCAAAACCCTCACCGGGAAGACCATCCTTTTGAATTGTAGCTTGTGTTATCATATTGCACAATTGGAGAGCAATTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTACATCCAAAAGGCAAGAATCAGCATAGAGCACTCT
TAAGGCCAAGGAAATCGGGCACAAAGTCATGTTACAGTACTTTGTATCCACAAGAACTTGGGGAGGCTGTAGTTGACAGACTTGATGCACTCAACGACCGAGGGCAGGAGCTTGGGAACCGGATAAGGGCAGTGGAGGAGCGGTTGTCTGCAATGGAACATGTGATAGATAGGCAGTGGCAGACAATCCAGTTGCTCCAG
GAGCAACTAGCGGCCCAGAGGGTAGCGTTGCGTGAGAATGGGATAGTGGCAGTGGAGGAACAGTTGTCTGCAATGGAAGAGGTAATAGAAAGGCAGAGACAGACAATCCAGTCGCTGCAACAGCAACTAGCAGCACAGAGGGCAGCATTTGACCAACAAATTCTGGTTTTGGCAAATCAGATCTGTGGCTTGAGGATAAG
ACGGAACCCCCCTTCAGCAATGGATAATATGGAACCCAAAGATGAAAACATGGAAGGTGATTCAAGCTAAGCAGAGAACGAAGGTGACTCCATCAGTGCTTTAAAAAACACAGTACGGTAGCAATTTTGGCTAAACAAAAGGTATGTTTCTTTGCCTATATGCTGGTCTAGTGGAGGACAATATGTCCGTATATATTTTA
GCAGCAGAATGCAGGAAAATAGGAATAGCTATAATGCCTTAATTATGCTGGAAAAGACTAAGACTCTGAATTTTGTATTTGGTCGGATTATCTGGTCATTTTGTACCAACCTGCCTCCAAGTTATGCTGAGGCTCTCTTTTTAATACAATACGAAGAAACTTCTTTCGAGTTAAACAAGATACAATGGAACAAGCCTTCA
AAGTGTATTGCTGTACACATTGTTGAAAACTGGATGGGCCAGAGTTTGTACCAATAATTGTCCTCCGTCGAGGTATATCTCTTTAAATGGTTTGATCTAAAATCTGCATTGTTGTCCACACACGTCTACGATTGACAACCTTGTCTTTGCTTTAAAAATTAAATGTTTTTTAATGTCAATATTAAACTTCCAAGGGAGAA
ACCACATAATCCCTGAAGGGGGCACTTCATAAATGTTTCTATTATCTAAAATATTACAAGTGGTTATTCCAGGGAGAAACTACATAATCCCTAAAGGGGGCACTTCATAAATGTTT

>TRINITY_DN121866_c0_g1_i1 from sample A2 / Example unspliced isoform:

TGCAGATCTTTGTCAAAACTCTCACTGGCAAGACCATCACTCTCGAGGTCGAGAGTTCTGATACAATTGACAATGTCAAGGCCATGATCCAGGACAAGGAAGGGATTCCACCTGACCAGCAGCGTCTGATCTTTGCTGGAAAGCAGCTGGAAGATGGCCGAACCCTTGCGGACTACAACATCCAAAAGGAATCCACCCTA
CACCTCGTTCTCCGTCTGAGAGGTGGCATGCAGATCTTTGTCAAAACCCTCACCGGGAAGACCATCCTTTTGAATTGTAGCTTGTGTTATCATATTGCACAATTGGAGAGCAAgttatgactccctaattattggcctttgttcatgttgtcttttgttgctgcagtttttgcttttttgtaaaaactgcgctcatgcaa
atatttcctataatcatattttttgtgggcaagtatggttgccttctctcatgcctttttttaattttgttgcatgtagTTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTACATCCAAAAGGCAAGAATCAGCATAGAGCACTCTTAAGGCCAAGGAAATCGGGCACAAAGTCATGTTA
CAGTACTTTGTATCCACAAGAACTTGGGGAGGCTGTAGTTGACAGACTTGATGCACTCAACGACCGAGGGCAGGAGCTTGGGAACCGGATAAGGGCAGTGGAGGAGCGGTTGTCTGCAATGGAACATGTGATAGATAGGCAGTGGCAGACAATCCAGTTGCTCCAGGAGCAACTAGCGGCCCAGAGGGTAGCGTTGCGTG
AGAATGGGATAGTGGCAGTGGAGGAACAGTTGTCTGCAATGGAAGAGGTAATAGAAAGGCAGAGACAGACAATCCAGTCGCTGCAACAGCAACTAGCAGCACAGAGGGCAGCATTTGACCAACAAATTCTGGTTTTGGCAAATCAGATCTGTGGCTTGAGGATAAGACGGAACCCCCCTTCAGCAATGGATAATATGGAA
CCCAAAGATGAAAACATGGAAGGTGATTCAAGCTAAGCAGAGAACGAAGGTGACTCCATCAGTGCTTTAAAAAACACAGTACGGTAGCAATTTTGGCTAAACAAAAGGTATGTTTCTTTGCCTATATGCTGGTCTAGTGGAGGACAATATGTCCGTATATATTTTAGCAGCAGAATGCAGGAAAATAGGAATAGCTATAA
TGCCTTAATTATGCTGGAAAAGACTAAGACTCTGAATTTTGTATTTGGTCGGATTATCTGGTCATTTTGTACCAACCTGCCTCCAAGTTATGCTGAGGCTCTCTTTTTAATACAATACGAAGAAACTTCTTTCGAGTTAAACAAGATACAATGGAACAAGCCTTCAAAGTGTATTGCTGTACACATTGTTGAAAACTGGA
TGGGCCAGAGTTTGTACCAATAATTGTCCTCCGTCGAGGTATATCTCTTTAAATGGTTTGATCTAAAATCTGCATTGTTGTCCACACACGTCTACGATTGACAACCTTGTCTTTGCTTTAAAAATTAAATGTTTTTTAATGTCAATATTAAACTTCCAAGGGAGAAACCACATAATCCCTGAAGGGGGCACTTCATAAAT
GTTTCTATTATCTAAAATATTACAAGTGGTTATTCCAGGGAGAAACTACATAATCCCTAAAGGGGGCACTTCATAAATGTTT

Intron 6 GTTATGACTC ... TTGCATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.14	247.7	89	37.6
A2	22.33	172.8	42	15.6
A3	25.06	215.8	58.7	27.4
A4	23.55	156.2	43	13.7
A5	24.45	115.1	30	17.2
A6	29.48	1427.6	269.1	49.4
A7	26.93	1196.5	250.9	95
A8	25.74	1175	225	86.9
A9	23.79	1250.9	257.2	116.5
A10	24.96	929.2	199.4	75.6

>TRINITY_DN128441_c3_g3_i2 from sample A1 / Example spliced isoform

GCAGATCTTTGTCAAAACCCTCACCGGGAAGACCATCCTTTTGAATTGTAGCTTGTGTTATCATATTGCACAATTGGAGAGCAATTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTACATCCAAAAGGCAAGAATCAGCATAGAGCACTCTTAAGGCCAAGGAAATCGGGCACAAAGTCA
TGTTACAGTACTTTGTATCCACAAGAACTTGGGGAGGCTGTAGTTGACAGACTTGATGCACTCAACGACCGAGGGCAGGAGCTTGGGAACCGGATAAGGGCAGTGGAGGAGCGGTTGTCTGCAATGGAACATGTGATAGATAGGCAGTGGCAGACAATCCAGTTGCTCCAGGAGCAACTAGCGGCCCAGAGGGTAGCGTT
GCGTGAGAATGGGATAGTGGCAGTGGAGGAACAGTTGTCTGCAATGGAAGAGGTAATAGAAAGGCAGAGACAGACAATCCAGTCGCTGCAACAGCAACTAGCAGCACAGAGGGCAGCATTTGACCAACAAATTCTGGTTTTGGCAAATCAGATCTGTGGCTTGAGGATAAGACGGAACCCCCCTTCAGCAATGGATAATA
TGGAACCCAAAGATGAAAACATGGAAGGTGATTCAAGCTAAGCAGAGAACGAAGGTGACTCCATCAGTGCTTTAAAAAACACAGTACGGTAGCAATTTTGGCTAAACAAAAGGTATGTTTCTTTGCCTATATGCTGGTCTAGTGGAGGACAATATGTCCGTATATATTTTAGCAGCAGAATGCAGGAAAATAGGAATAGC
TATAATGCCTTAATTATGCTGGAAAAGACTAAGACTCTGAATTTTGTATTTGGTCGGATTATCTGGTCATTTTGTACCAACCTGCCTCCAAGTTATGCTGAGGCTCTCTTTTTAATACAATACGAAGAAACTTCTTTCGAGTTAAACAAGATACAATGGAACAAGCCTTCAAAGTGTATTGCTGTACACATTGTTGAAAA
CTGGATGGGCCAGAGTTTGTACCAATAATTGTCCTCCGTCGAGGTATATCTCTTTAAATGGTTTGATCTAAAATCTGCATTGTTGTCCACACACGTCTACGATTGACAACCTTGTCTTTGCTTTAAAAATTAAATGTTTTTTAATGTCAATATTAAACTTCCAAGGGAGAAACCACATAATCCCTGAAGGGGGCACTTCA
TAAATGTTTCTATTATCTAAAATATTACAAGTGGTTATTCCAGGGAGAAACTACATAATCCCTAAAGGGGGCACTTCATAAATGTTT

>TRINITY_DN128441_c3_g3_i4 from sample A1 / Example unspliced isoform:

GCAGATCTTTGTCAAAACCCTCACCGGGAAGACCATCCTTTTGAATTGTAGCTTGTGTTATCATATTGCACAATTGGAGAGCAAgttatgactccctaattattggcctttgttcatgttgtcttttgttgctgcagtttttgcttttttgtaaaaactgcgctcatgcaaatatttcctataatcatattttttgtggg
caagtatggttgccttctctcatgccttttttaattttgttgcatgtagTTTCTAAATTTGAGGGGCAAATTGCAATTACTAGACAGTATACACGGTTCCTACATCCAAAAGGCAAGAATCAGCATAGAGCACTCTTAAGGCCAAGGAAATCGGGCACAAAGTCATGTTACAGTACTTTGTATCCACAAGGTAGGCTGAT
ATCAATTTCATCCTATGGATGCTTGACTTTATATGCAGAGAAGTTGAAGATGATTTGACTTGGTTTGAATTTTAAATAGCATTCAGAGTTAACTTTATATGCACGCTGTTTATGATTTGTGGAGGTTATTGAATTTAGAGGAGAGGGGTATCATTCGGGGATAATCAGATTGCAAAGCAGGTTTCGTTTTATCTGTTTAA
TGGCAAAACTGGAGTTAGATCAAGTTATAGACTAAATTTGTTAACATTAAAACAATGAATGCTGTTTGTTAATGGTACAATGGGATCAAAATAATGAATACAAGAAATATATTCAGTGTATGCATGCGTTATAGTCTTTTAATTATGTGCCCTGTTGCTTTGTATTGCAACTAAGGCTTTAGATTGAATAGCCACTTATT
GTGTTTTGTTCTTATTTTTCTAACTATGCTAGAATACTTAACAAACTGAGAAACTAAATAGCTGTTGAAACTCTTATTGAAGAACTTGGGGAGGCTGTAGTTGACAGACTTGATGCACTCAACGACCGAGGGCAGGAGCTTGGGAACCGGATAAGGGCAGTGGAGGAGCGGTTGTCTGCAATGGAACATGTGATAGATAG
GCAGTGGCAGACAATCCAGTTGCTCCAGGAGCAACTAGCGGCCCAGAGGGTAGCGTTGCGTGAGAATGGGATAGTGGCAGTGGAGGAACAGTTGTCTGCAATGGAAGAGGTAATAGAAAGGCAGAGACAGACAATCCAGTCGCTGCAACAGCAACTAGCAGCACAGAGGGCAGCATTTGACCAACAAATTCTGGTTTTGG
CAAATCAGATCTGTGGCTTGAGGATAAGACGGAACCCCCCTTCAGCAATGGATAATATGGAACCCAAAGATGAAAACATGGAAGGTGATTCAAGCTAAGCAGAGAACGAAGGTGACTCCATCAGTGCTTTAAAAAACACAGTACGGTAGCAATTTTGGCTAAACAAAAGGTATGTTTCTTTGCCTATATGCTGGTCTAGT
GGAGGACAATATGTCCGTATATATTTTAGCAGCAGAATGCAGGAAAATAGGAATAGCTATAATGCCTTAATTATGCTGGAAAAGACTAAGACTCTGAATTTTGTATTTGGTCGGATTATCTGGTCATTTTGTACCAACCTGCCTCCAAGTTATGCTGAGGCTCTCTTTTTAATACAATACGAAGAAACTTCTTTCGAGTT
AAACAAGATACAATGGAACAAGCCTTCAAAGTGTATTGCTGTACACATTGTTGAAAACTGGATGGGCCAGAGTTTGTACCAATAATTGTCCTCCGTCGAGGTATATCTCTTTAAATGGTTTGATCTAAAATCTGCATTGTTGTCCACACACGTCTACGATTGACAACCTTGTCTTTGCTTTAAAAATTAAATGTTTTTTA
ATGTCAATATTAAACTTCCAAGGGAGAAACCACATAATCCCTGAAGGGGGCACTTCATAAATGTTTCTATTATCTAAAATATTACAAGTGGTTATTCCAGGGAGAAACTACATAATCCCTAAAGGGGGCACTTCATAAATGTTT