sp|Q8W4I6|BPG2_ARATH ----> GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic

Intron 1 GTAAGACTTG ... TTGTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.03	66.1	33.5	12.9
A2	23.93	74.9	23.9	24.5
A3	21.25	63.9	16.6	14.8
A4	32.21	56.9	27.1	13.3
A5	24.38	21.5	6.8	10.9
A6	17.24	276.8	56.8	56.3
A7	19.78	363.4	89.3	76
A8	16.76	418.8	81.4	103.1
A9	14.39	372.4	60.4	87.4
A10	17.74	337.6	70.4	92.4

>TRINITY_DN44901_c2_g1_i5 from sample A6 / Example spliced isoform

CTGAAACTTAAAGTAAATAGCATGTTTTGCTAGAAACATCTCTCAGACGTCTTATTCTAATTACTGTTTAATGTTAGTGTAAATTTATTTGTTCTATAGCAATATGAGAGTTTATGGCGGATATTCTATTAAATTTTATCTTATATCTTCTGTAGGAGGAGATAACTGGAGAGTGGAAAAGGTGCACAAGCCGCTTTAAG
TATATTGAGGAGGTTCACAGTGTAGTTGAATATCATCGCAATCTTACCAGGAAAGGCTACCGTGCTTTAATCTTCAGGTCCATTAATACAAATATAGTTAAATGTCTGTGTTTACATTCATAGTTTCTTGTGATTGCGAATACGACTTTATGCTGACAGAATTGCTATGCATGAGCTTGTAAATGTTCAGCATCGGATTT
AACTAGCTTTGATGTCATGACAGTGGTGACTATGATATGGTTTTTCCATTCATTGGAACTGAAGCATGGATCAGAACATTGAACTATACAATAGTTGATGATTGGAGGTCTTGGTGGGTGGATCGACAAATTGCAGGATACACAAGATTATATGACAACAACCTAACATTTGCAACAGTCAAGGGAGCTGGACATACAGC
TCCATAATACAAGCCTCGACAATCATTTGTGATGTTCAAGCAATGGAACTCTGGAGAACGTCTGTAAATTGTGAACACCCATCTTAATAGTTCATCCTATTATTTTGCAAGAGACCTCTTCTTTACTATTGCCTTGGCAGTTTACTTTCCTTTCTTTTACAAACTCTGTATTAGCGTTCTTATGTAAATACTCCTTTTCT
GAGGAATTATGACCTGCTCTGTTTCTTATCACAAGATGCCAATTCAATGTTCAAGTGTAGTTTATTTACTGGTTCTGTAACAAACCTTCTTCTCAGCAATTTAAGTTGATGTGAGTCCACTGTAATTATTTTGGGGCTTTTATATATTTTGGTAGAGTTCTCATAGGGCTAATGCGACTACCCAGAAGCCTTATAACTTC
TGCTGGCAAATGAAGGTATGAGTTTGTGGATGATGCAATCATGATGTTGTCCTTACTGGCATTGCTACTTTAGTACATTGTACGTAAGAAATTGTAGTTGCAATATTGAATAAATCTCTTATACATTCTTATGTATACTTGTGAGTTAATTGAGATGATTTTGGAATGTGAAAATAC

>TRINITY_DN44901_c2_g1_i1 from sample A6 / Example unspliced isoform:

TGGGTGGATCGACAAATTGCAGGgtaagacttggagctcagaatcctggcagtagtctttaaacatcaattgtatattatgtatgtttgaaaaggaaagaacaatatacatgcttttacggtagagaacccttctctaacctgcccttctattctatagagaggcagagtagagattttcgttgcatagtttcttgtagt
caaagaattgaacaatggaaatatacgacattatggttggtgccttgaccatgtcatgcaagagtaaggttttgtcaaacaattgaacaatgaaaatagatggtattatggttagtgtcttgatcaggtcatgcaagtataaggtttttccaaatgattgaacaataaaaacagatggcaccatggttagtgccttgatc
aggtcatgtaaggagaaggttaaatttctaacccctccttccttatcgttcagtaaaagggtcatttgcttgaggtaagaaacattgatgccggatcttccattttgtgtgcagATACACAAGATTATATGACAACAACCTAACATTTGCAACAGTCAAGGGAGCTGGACATACAGCTCCATAATACAAGCCTCGACAAT
CATTTGTGATGTTCAAGCAATGGAACTCTGGAGAACGTCTGTAAATTGTGAACACCCATCTTAATAGTTCATCCTATTATTTTGCAAGAGACCTCTTCTTTACTATTGCCTTGGCAGTTTACTTTCCTTTCTTTTACAAACTCTGTATTAGCGTTCTTATGTAAATACTCCTTTTCTGAGGAATTATGACCTGCTCTGTT
TCTTATCACAAGATGCCAATTCAATGTTCAAGTGTAGTTTATTTACTGGTTCTGTAACAAACCTTCTTCTCAGCAATTTAAGTTGATGTGAGTCCACTGTAATTATTTTGGGGCTTTTATATATTTTGGTAGAGTTCTCATAGGGCTAATGCGACTACCCAGAAGCCTTATAACTTCTGCTGGCAAATGAAGGTATGAGT
TTGTGGATGATGCAATCATGATGTTGTCCTTACTGGCATTGCTACTTTAGTACATTGTACGTAAGAAATTGTAGTTGCAATATTGAATAAATCTCTTATACATTCTTATGTATACTTGTGAGTTAATTGAGATGATTTTGGAATGTGAAAATAC

Intron 2 ATTTAGGGCT ... TTAATAGGAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.39	10	8	5
A2	35	13	9	5
A3	45.45	12	9	11
A4	44	7	7	4
A5	25	3	1	1
A6	42.86	2	2	1
A7	45.45	3	3	2
A8	47.37	10	8	10
A9	33.33	2	1	1
A10	40	3	1	3

>TRINITY_DN44803_c0_g2_i11 from sample A8 / Example spliced isoform

TGGGTACTGCGTTATTCCCGAATTCTTCACCATACATATTGTTTTAAGCTTATTAGAGAGGATGGACATGAAGCCGTTTGGAGTTTGGGCCGCCTGGAGTTTGGGCCATGTTACGGCTGGTTTTTTCTGGTTGGTAAACCTGTTGTTACCTGGCCTCATGTTTCCTGACTGGTTTATGGGCTTTGTTTTTGTCTTCTTTG
CACTTGCTGTTGACCTGAAACCTGTACTTAGTGGACGGACCCTGGGACTTGAGTCAAGCTTGTCTTCTATGACAAGCCCAGTACCAACCACGGGTTCTTCACTAGGGGGTCAATATTATCCCACATCCGTTCATATTGGGGGTATTAATTAAACATGAAAACCATAATATTATATTGCTTATATATTAAGGGTGGTTAGA
GTTTTTTAATTTAACAGAGCAATACACAATATGTTGAGGTATATTATATATGGTCGGGCTGCCAAATGTGCTCCGAAATGGCAATGAAAATGCTGCAGGGAAATCGAAATCGCGTGGCGGCCGGAGGCCAGGGTTTTCTGTGGTGAAGCCCCTGTGGTACCAAGAGCTCTCGCAGGGCTGTTCTGTTCTTTTTTTGCATA
TCGGCGACTGCGTGGCGGGAGAGCTCAAGGAGGCGAACTTGAAGCCCATTGAAACGGGAAGCAATGTGAAGATAGGGCTTCTTGGTACAGAGGAGACCTTAAGAGCGGGGTTTTATCAAGATAGGCTTAGCAATCAGGTAAGCTTGCTGTCAATAGTTTTTATATAGGTGCATAATCATAATTTCAGTGTGCTTGAATTG
GGTTTCGAAAAACAGATCAGGATATAGTCAGAAATGCTGTATTTGGGCCTTTACGAATAGCTCATAATTGTTCCAGTGTGGCTGCATCTGGTTTTGGGGACGGGTCGTGGTAATTGATATCTGACAGTTCAACTACAAATTGGTTACCAAGATAGACCATGCCCTACTATGGTAGATTGCAATAGGTCCTCTGCTCCTCA
TTTATGGATTTGAAACCTGTTTTATTTGCAGTATAGCAAATTTGTATCGTACATTTTCCTATAGGCTTTTGACAACCGACATATCCAACAAAAGAGGAGGATTACCTTCATACGTGTCGTATATCTCAATGCATACAGCCTTTAAACTACTAGGATTTAATAAGGAATTTATTAAATTCACAATTTTTTGGTTTGCTTTT
GGTGAAGGGCATTGAGGTTCTGGAGTTGGATTTTGTAAAGTTGTCTTTCATAGTTGCAGTTTTACAGATGTTTTTGTGTAAAGTTGCATATTTTCTTTCACCTTCTGTCATAGTTGCAGCCCTTACAGTAGATCTTTTTGTATAGACTCTCCTAATAAAATGATTCATATTGCAAAACAGGACAAACATCGGCTTTCATA
CATTTGGGCAAATAATCCGTATGTGAGGAAAGCAATTCATGCTCAGTCGGAGGAGATAACTGGAGAGTGGAAAAGGTGCACAAGCCGCTTTAAGTATATTGAGGAGGTTCACAGTGTAGTTGAATATCATCGCAATCTTACCAGGAAAGGCTACCGTGCTTTAATCTTCAGTGGTGACTATGATATGGTTTTTCCATTCA
TTGGAACTGAAGCATGGATCAGAACATTGAACTATACAATAGTTGATGATTGGAGGTCTTGGTGGGTGGATCGACAAATTGCAGG

>TRINITY_DN44803_c0_g2_i7 from sample A8 / Example unspliced isoform:

GCCCATTGAAACGGGAAGCAATGTGAAGATAGGGCTTCTTGGTACAGAGGAGACCTTAAGAGCGGGGTTTTATCAAGATAGGCTTAGCAATCAGGTAAGCTTGCTGTCAATAGTTTTTATATAGGTGCatttagggcttatttaataggacATAATCATAATTTCAGTGTGCTTGAATTGGGTTTCGAAAAACAGATCAG
GATATAGTCAGAAATGCTGTATTTGGGCCTTTACGAATAGCTCATAATTGTTCCAGTGTGGCTGCATCTGGTTTTGGGGACGGGTCGTGGTAATTGATATTGGGAATATAGGTGAGTGGATACAGAATCGTCTTGAGAATTGTCCAAAGCTACGGAGAGTTCTACTTAGCCCTAAAATATATACCAAAAGAGAAGCACAA
TGAGAGTTCCACTCTTAGTTAGCAAACTCATACTCGCTGAGGCATGTGACATGGGGTACTTGGCATGTTCATC

Intron 3 GTATGGCATC ... TTTATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.69	21	33	55
A2	62.5	21	31	39
A3	74.05	17	52	45
A4	72.6	10	30	23
A5	63.64	10	25	10
A6	62.03	15	9	40
A7	63	37	64	62
A8	78.42	15	19	90
A9	77.78	10	25	45
A10	63.3	20	38	31

>TRINITY_DN108474_c0_g1_i5 from sample A5 / Example spliced isoform

ATTTGCTAAATTTTTTAGCATACCACAGGCCAGAGTACAGAAATACCCTAAACCCCAGCCCCTGATAACCTTCCTGCCATTGTAGCATCGTGATCCTGTAATATCAACCCAAGGCGATCATGTTGCAAAGGGTTGTAAAGCTCAATGTCAAGCTGCTCGCTGCATCTCGGACCAGAAAATGCTTTCCAACATCAAAATCA
TCAGCTCATTGTTCTTATTTGAAGCATGAAAGAACCGGCAATGCAGAGGCCGCAGGTATTGAGAAGAACTCACGCTGTGTCATATGAACAATACTGCAAAAGTCTTTCATCAACTATCTTTAATCTTCGCAAAGTCTGGCATCCAGAGATGTACACTCTTGCAATGAACAACCGAATTATTGATGCCAAAAAATTCTGAG
AAGAAGGAGGAGATAACTGGAGAGTGGAAAAGGTGCACAAGCCGCTTTAAGTATATTGAGGAGGTTCACAGTGTAGTTGAATATCATCGCAATCTTACCAGGAAAGGCTACCGTGCTTTAATCTTCAGGTCCATTAATACAAATATAGTTAAATGTCTGTGTTTACATTCATAGTTTCTTGTGATTGCGAATACGACTTT
ATGCTGACAGAATTGCTATGCATGAGCTTGTAAATGTTCAGCATCGGATTTAACTAGCTTTGATGTCAT

>TRINITY_DN108474_c0_g1_i3 from sample A5 / Example unspliced isoform:

ATTTGCTAAATTTTTTAGCATACCACAGGCCAGAGTACAGAAATACCCTAAACCCCAGCCCCTGATAACCTTCCTGCCATTGTAGCATCGTGATCCTGTAATATCAACCCAAGGCGATCATGTTGCAAAGGGTTGTAAAGCTCAATGTCAAGCTGCTCGCTGCATCTCGGACCAGAAAATGCTTTCCAACATCAAAATCA
TCAGCTCATTGTTCTTATTTGAAGCATGAAAGAACCGGCAATGCAGAGGCCGCAGGTATTGAGAAGAACTCACGgtatggcatctttcccttctgtcaatctcgaataatacgaggcatttcctctgatgcatctgccattaaagattttacgtcatcaacaaacccccccgaaaacaatagtgtaggaatccaactgca
aaaatcgctgcaatggggccgaagcgagggccgcgaaagtgattccgagcgtttagtctgcctgggatgcggagtttacatgcagaacgagcagccgatggtgcccggattcttcactaaaccctcagagaaagttaaaaagaaaagggtgtgggttgatgtcaaaggagagaatttaggagatgaaatctggcttaatc
cagacgacaaaagagcaattagtaatcagattaaggtccaggaacaggaccccaatttgtctgaagagggtaaaaataatgtaaaccctgagaaagctgaaacgctggtggtttgcgcccggtgccattccttgcgccactatgggaaagtgaaggacgagagcgtagaaaatttgctgccggattttgattttgaagtt
acagtgggagccagattgatgaaaacaaccggcagcagatcgactgtagtgatggtggtcgataccgtggactttgacgggtcttttcctcggcgtgtggcggtgttagtgtcgcgtgcgatggaagaaggggcgcatgtctggaaggaaggaaagtccggtaacattccccgtttagtgctcgttgccacgaagctcga
tcttcttcctactcaggttacgccacagaggttagagcattgggttcgccggcagtccaggttaggtggggcagaaaggttacatggagtccatcttgtcagttcccacaaaggctgggggattcagaacttggccgagcatatcaagcagctggccggtgctcgagggaatgtgtgggtgatcggtgcgcagaatgtgg
gcaaatcgactttgattaatgcgattggcaagcacgttggagggaaggttgcccagctcacggaagctccggttccaggcactacattggggattctgaggatcgaagggattctgcctgacaaggccaagttgtatgacacgccgggccttctgcatccatatcagatatcaacacgattgaacagagaagagcagaag
atggttcagattaaaaaagagcttaggcccaggagctacaggttaactgtgggtcaatgtgtacatgttggcggattaatgagggttgacatcgaagagtcttctgtgcagtcattctatgtaactgtctgggcctctccgtttattcccttgcatatggggagggttgagaatgcggaagtgataagagaaaaccattt
cggactacagttgcagcctcccattggggtggaaagagtctccgagcttggtgaatggaggcgaagggaattcaaagtgtcaggaaacagttgggatttcaattctatggatatagctactgcaggtcttggttggttgtcatttggcctgaaaggtgatgcaactttggctgtgtggacttatgatggtgtggatgtaa
aggaacggagtgctttgatcctcgacagagcacgaatctttgaagacactggattcacagtttctaatgtagtatccaaggcggataagaaaaaatcaaatgatgctaaagtaaacaagaaaagggatacaaaaaacaagaaaagcaagaatgcaattggagagatttgacaacattgaagaaatgccttggcctatagt
ttttttttgaaccggaaaactcgataagggtccaaattatgcaaaattatgaccacaatttgcaacagagatatgtggcacacttaaaatcaagaatgcatttgcgcctttgttccaatgcacctgcacggagtgcaagcttccaaagtttcaaagtgcaactttttgctcattctgcagtcacaaaacaccaacatctt
atgaatacattttatatttcaaattagaaacagaggagggcatcatcgaatgcacattacgaggatcatagttaaaataattgtgtccatttattgcagCTGTGTCATATGAACAATACTGCAAAAGTCTTTCATCAACTATCTTTAATCTTCGCAAAGTCTGGCATCCAGAGATGTACACTCTTGCAATGAACAACCGA
ATTATTGATGCCAAAAAATTCTGAGAAGAAGGTACAAAAGTAGGATTAGCAA

Intron 4 GTAGAAATTG ... TATTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.94	40.1	5.2	9.1
A2	10.33	57.6	7.2	8.9
A3	6.8	59.6	4.2	11
A4	9.47	40.8	4.1	4.1
A5	23.28	15.4	6.1	1.3
A6	0.82	206.1	1.7	15.6
A7	0.67	252.7	1.7	20.9
A8	1.92	369.6	7.2	45
A9	1.38	280.7	3.8	19.7
A10	1.24	267.6	3.3	17.3

>TRINITY_DN108664_c0_g5_i5 from sample A4 / Example spliced isoform

ATTTGCTAAATTTTTTAGCATACCACAGGCCAGAGTACAGAAATACCCTAAACCCCAGCCCCTGATAACCTTCCTGCCATTGTAGCATCGCGATCCTGTAATATCAACCCAAGGCGATCATGTTGCAAAGGGTTGTAAAGCTCAATGTCAAGCTGCTCGCTGCATCTCGGACCAGAAAATGCTTTCCAACATCAAAATCA
TCAGCTCATTGTTCTTATTTGAAGCATGAAAGAACCGGCAATGCAGAGGCCGCAGGTATTGAGAAGAACTCACGGTATGGCATCTTTCCCTTCTGTCAATCTCGAATAATACGAGGCATTTCCTCTGATGCATCTGCCATTAAAGATTTTACGTCATCAACAAACCCCCCCGAAAACAATAGTGTAGGAATCCAACTGCA
AAAATCGCTGCAATGGGGCCGAAGCGAGGGCCGCGAAAGTGATTCCGAGCGTTTAGTCTGCCTGGGATGCGGAGTTTACATGCAGAACGAGCAGCCGATGGTGCCCGGATTCTTCACTAAACCCTCAGAGAAAGTTAAAAAGAAAAGGGTGTGGGTTGATGTCAAAGGAGAGAATTTAGGAGATGAAATCTGGCTTAATC
CAGACGACAAAAGAGCAATTAGTAATCAGATTAAGGTCCAGGAACAGGACCCCAATTTGTCTGAAGAGGGTAAAAATAATGTAAACCCTGAGAAAGCTGAAACGCTGGTGGTTTGCGCCCGGTGCCATTCCTTGCGCCACTATGGGAAAGTGAAGGACGAGAGCGTAGAAAATTTGCTGCCGGATTTTGATTTTGAAGTT
ACAGTGGGAGCCAGATTGATGAAAACAACCGGCAGCAGATCGACTGTAGTGATGGTGGTCGATACCGTGGACTTTGACGGGTCTTTTCCTCGGCGTGTGGCGGTGTTAGTGTCGCGTGCGATGGAAGAAGGGGCGCATGTCTGGAAGGAAGGAAAGTCCGGTAACATTCCCCGTTTAGTGCTCGTTGCCACGAAGCTCGA
TCTTCTTCCTACTCAGGTTACGCCACAGAGGTTAGAGCATTGGGTTCGCCGGCAGTCCAGGTTAGGTGGGGCAGAAAGGTTACATGGAGTCCATCTTGTCAGTTCCCACAAAGGCTGGGGGATTCAGAACTTGGCCGAGCATATCAAGCAGCTGGCCGGTGCTCGAGGGAATGTGTGGGTGATCGGTGCGCAGAATGTGG
GCAAATCGACTTTGATTAATGCGATTGGCAAGCACGTTGGAGGGAAGGTTGCCCAGCTCACGGAAGCTCCGGTTCCAGGCACTACATTGGGGATTCTGAGGATCGAAGGGATTCTGCCTGACAAGGCCAAGTTGTATGACACGCCGGGCCTTCTGCATCCATATCAGATATCAACACGATTGAACAGAGAAGAGCAGAAG
ATGGTTCAGATTAAAAAAGAGCTTAGGCCCAGGAGCTACAGGTTAACTGTGGGTCAATGTGTACATGTTGGCGGATTAATGAGGGTTGACATCGAAGAGTCTTCTGTGCAGTCATTCTATGTAACTGTCTGGGCCTCTCCGTTTATTCCCTTGCATATGGGGAGGGTTGAGAATGCGGAAGTGATAAGAGAAAACCATTT
CGGACTACAGTTGCAGCCTCCCATTGGGGTGGAAAGAGTCTCCGAGCTTGGTGAATGGAGGCGAAGGGAATTCAAAGTGTCAGGAAACAGTTGGGATTTCAATTCTATGGATATAGCTACTGCAGGTCTTGGTTGGTTGTCATTTGGCCTGAAAGGTGATGCAACTTTGGCTGTGTGGACTTATGATGGTGTGGATGTAA
ACGAACGGAGTGCTTTGATCCTCGACAGAGCACGAATCTTTGAAGACACTGGATTCACAGTTTCTAATGTAGTATCCAAGGCGGATAAGAAAAAATCAAATGATGCTAAAGTAAACAAGAAAAGGGATACAAAAAACAAGAAAAGCAAGAATGCAATTGGAGAGATTTGACAACATTGAAGAAATGCCTTGGCCTATAGT
TTTTTTTTGAACCGGAAAACTCGATAAGGGTCCAAATTATGCAAAATTATGACCACAATTTGCAACAGAGATATGTGGCACACTTAAAATCAAGAATGCATTTGCGCCTTTGTTCCAATGCACCTGCACGGAGTGCAAGCTTCCAAAGTTTCAAAGTGCAACTTTTTGCTCATTCTGCAGTCACAAAACACCAACATCTT
ATGAATACATTTTATATTTCAAATTAGAAACAGAGGAGGGCATCATCGAATGCACATTACGAGGATCATAGTTAAAATAATTGTGTCCATTTATTGCAGCTGTGTCATATGAACAATACTGCAAAAGTCTTTCATCAACTATCTTTAATCTTCGCAAAGTCTGGCATCCAGAGATGTACACTCTTGCAATGAACAACCGA
ATTATTGATGCCAAAAAATTCTGAGAAGAAGGAGGAGATAACTGGAGAGTGGAAAAGGTGCACAAGCCGCTTTAAGTATATTGAGGAGGTTCACAGTGTAGTTGAATATCATCGCAATCTTACCAGGAAAGGCTACCGTGCTTTAATCTTCAGTGGTGACTATGATATGGTTTTTCCATTCATTGGAACTGAAGCATGGA
TCAGAACATTGAACTATACAATAGTTGATGATTGGAGGTCTTGGTGGGTGGATCGACAAATTGCAGGATACACAAGATTATATGACAACAACCTAACATTTGCAACAGTCAAGGGAGCTGGACATACAGCTCCATAATACAAGCCTCGACAATCATTTGTGATGTTCAAGCAATGGAACTCTGGAGAACGTCTGTAAATT
GTGAACACCCATCTTAATAGTTCATCCTATTATTTTGCAAGAGACCTCTTCTTTACTATTGCCTTGGCAGTTTACTTTCCTTTCTTTTACAAACTCTGTATTAGCGTTCTTATGTAAATACTCCTTTTCTGAGGAATTATGACCTGCTCTGTTTCTTATCACAAGATGCCAATTCAATGTTCAAGTGTAGTTTATTTACT
GGTTCTGTAACAAACCTTCTTCTCAGCAATTTAAGTTGATGTGAGTCCACTGTAATTATTTTGGGGCTTTTATATATTTTGGTAGAGTTCTCATAGGGCTAATGCGACTACCCAGAAGCCTTATAACTTCTGCTGGCAAATGAAGGTATGAGTTTGTGGATGATGCAATCATGATGTTGTCCTTACTGGCATTGCTACTT
TAGTACATTGTACGTAAGAAATTGTAGTTGCAATATTGAATAAATCTCTTATACATTCTTATGTATACTCGTGAGTTAATTGAGATGATTTTGGAATGTGAAAAGACCATGGGCATTTCTTCACTAACTCTGCTAACTATGTTGTGAAAGTTGAGTGTTTAGAGGTTTTTTACTCATATGAATTAGGTCTGGAAAGTATG
TAAGCTTTTCACTTACGCTTTACCTTAATAGGATATGCTCTGGTTTGATATTACCAAACTTTGTTTGAGTTTTTCTTCTCTGCA

>TRINITY_DN108664_c0_g5_i4 from sample A4 / Example unspliced isoform:

CCTAACATTTGCAACAGTCAAGgtagaaattgtagtcctagctataccctgtgatatggttttctcagttttatttcatgacatagagaaaatggaggatgattgatccatctcaggtatgcaataactaataatgagctgaatattctgcagGGAGCTGGACATACAGCTCCATAATACAAGCCTCGACAATCATTTGT
GATGTTCAAGCAATGGAACTCTGGAGAACGTCTGTAAATTGTGAACACCCATCTTAATAGTTCATCCTATTATTTTGCAAGAGACCTCTTCTTTACTATTGCCTTGGCAGTTTACTTTCCTTTCTTTTACAAACTCTGTATTAGCGTTCTTATGTAAATACTCCTTTTCTGAGGAATTATGACCTGCTCTGTTTCTTATC
ACAAGATGCCAATTCAATGTTCAAGTGTAGTTTATTTACTGGTTCTGTAACAAACCTTCTTCTCAGCAATTTAAGTTGATGTGAGTCCACTGTAATTATTTTGGGGCTTTTATATATTTTGGTAGAGTTCTCATAGGGCTAATGCGACTACCCAGAAGCCTTATAACTTCTGCTGGCAAATGAAGGTATGAGTTTGTGGA
TGATGCAATCATGATGTTGTCCTTACTGGCATTGCTACTTTAGTACATTGTACGTAAGAAATTGTAGTTGCAATATTGAATAAATCTCTTATACATTCTTATGTATACTCGTGAGTTAATTGAGATGATTTTGGAATGTGAAAAGACCATGGGCATTTCTTCACTAACTCTGCTAACTATGTTGTGAAAGTTGAGTGTTT
AGAGGTTTTTTACTCATATGAATTAGGTCTGGAAAGTATGTAAGCTTTTCACTTACGCTTTACCTTAATAGGATATGCTCTGGTTTGATATTACCAAACTTTGTTTGAGTTTTTCTTCTCTGCA

Intron 5 GTAAGCTTGC ... TGCAAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	87.88	2	12	17
A2	65.22	4	12	3
A3	51.35	9	15	4
A4	76	3	10	9
A5	41.18	5	5	2
A6	32.35	23	21	1
A7	33.33	24	17	7
A8	43.86	16	21	4
A9	33.33	9	7	2
A10	33.33	9	5	4

>TRINITY_DN44803_c0_g2_i1 from sample A8 / Example spliced isoform

TGGGTACTGCGTTATTCCCGAATTCTTCACCATACATATTGTTTTAAGCTTATTAGAGAGGATGGACATGAAGCCGTTTGGAGTTTGGGCCGCCTGGAGTTTGGGCCATGTTACGGCTGGTTTTTTCTGGTTGGTAAACCTGTTGTTACCTGGCCTCATGTTTCCTGACTGGTTTATGGGCTTTGTTTTTGTCTTCTTTG
CACTTGCTGTTGACCTGAAACCTGTACTTAGTGGACGGACCCTGGGACTTGAGTCAAGCTTGTCTTCTATGACAAGCCCAGTACCAACCACGGGTTCTTCACTAGGGGGTCAATATTATCCCACATCCGTTCATATTGGGGGTATTAATTAAACATGAAAACCATAATATTATATTGCTTATATATTAAGGGTGGTTAGA
GTTTTTTAATTTAACAGAGCAATACACAATATGTTGAGGTATATTATATATGGTCGGGCTGCCAAATGTGCTCCGAAATGGCAATGAAAATGCTGCAGGGAAATCGAAATCGCGTGGCGGCCGGAGGCCAGGGTTTTCTGTGGTGAAGCCCCTGTGGTACCAAGAGCTCTCGCAGGGCTGTTCTGTTCTTTTTTTGCATA
TCGGCGACTGCGTGGCGGGAGAGCTCAAGGAGGCGAACTTGAAGCCCATTGAAACGGGAAGCAATGTGAAGATAGGGCTTCTTGGTACAGAGGAGACCTTAAGAGCGGGGTTTTATCAAGATAGGCTTAGCAATCAGGACAAACATCGGCTTTCATACATTTGGGCAAATAATCCGTATGTGAGGAAAGCAATTCATGCT
CAGTCGGAGGAGATAACTGGAGAGTGGAAAAGGTGCACAAGCCGCTTTAAGTATATTGAGGAGGTTCACAGTGTAGTTGAATATCATCGCAATCTTACCAGGAAAGGCTACCGTGCTTTAATCTTCAGTGGTGACTATGATATGGTTTTTCCATTCATTGGAACTGAAGCATGGATCAGAACATTGAACTATACAATAGT
TGATGATTGGAGGTCTTGGTGGGTGGATCGACAAATTGCAGGATACACAAGATTATATGACAACAACCTAACATTTGCAACAGTCAAGG

>TRINITY_DN44803_c0_g2_i11 from sample A8 / Example unspliced isoform:

TGGGTACTGCGTTATTCCCGAATTCTTCACCATACATATTGTTTTAAGCTTATTAGAGAGGATGGACATGAAGCCGTTTGGAGTTTGGGCCGCCTGGAGTTTGGGCCATGTTACGGCTGGTTTTTTCTGGTTGGTAAACCTGTTGTTACCTGGCCTCATGTTTCCTGACTGGTTTATGGGCTTTGTTTTTGTCTTCTTTG
CACTTGCTGTTGACCTGAAACCTGTACTTAGTGGACGGACCCTGGGACTTGAGTCAAGCTTGTCTTCTATGACAAGCCCAGTACCAACCACGGGTTCTTCACTAGGGGGTCAATATTATCCCACATCCGTTCATATTGGGGGTATTAATTAAACATGAAAACCATAATATTATATTGCTTATATATTAAGGGTGGTTAGA
GTTTTTTAATTTAACAGAGCAATACACAATATGTTGAGGTATATTATATATGGTCGGGCTGCCAAATGTGCTCCGAAATGGCAATGAAAATGCTGCAGGGAAATCGAAATCGCGTGGCGGCCGGAGGCCAGGGTTTTCTGTGGTGAAGCCCCTGTGGTACCAAGAGCTCTCGCAGGGCTGTTCTGTTCTTTTTTTGCATA
TCGGCGACTGCGTGGCGGGAGAGCTCAAGGAGGCGAACTTGAAGCCCATTGAAACGGGAAGCAATGTGAAGATAGGGCTTCTTGGTACAGAGGAGACCTTAAGAGCGGGGTTTTATCAAGATAGGCTTAGCAATCAGgtaagcttgctgtcaatagtttttatataggtgcataatcataatttcagtgtgcttgaattg
ggtttcgaaaaacagatcaggatatagtcagaaatgctgtatttgggcctttacgaatagctcataattgttccagtgtggctgcatctggttttggggacgggtcgtggtaattgatatctgacagttcaactacaaattggttaccaagatagaccatgccctactatggtagattgcaataggtcctctgctcctca
tttatggatttgaaacctgttttatttgcagtatagcaaatttgtatcgtacattttcctataggcttttgacaaccgacatatccaacaaaagaggaggattaccttcatacgtgtcgtatatctcaatgcatacagcctttaaactactaggatttaataaggaatttattaaattcacaattttttggtttgctttt
ggtgaagggcattgaggttctggagttggattttgtaaagttgtctttcatagttgcagttttacagatgtttttgtgtaaagttgcatattttctttcaccttctgtcatagttgcagcccttacagtagatctttttgtatagactctcctaataaaatgattcatattgcaaaacagGACAAACATCGGCTTTCATA
CATTTGGGCAAATAATCCGTATGTGAGGAAAGCAATTCATGCTCAGTCGGAGGAGATAACTGGAGAGTGGAAAAGGTGCACAAGCCGCTTTAAGTATATTGAGGAGGTTCACAGTGTAGTTGAATATCATCGCAATCTTACCAGGAAAGGCTACCGTGCTTTAATCTTCAGTGGTGACTATGATATGGTTTTTCCATTCA
TTGGAACTGAAGCATGGATCAGAACATTGAACTATACAATAGTTGATGATTGGAGGTCTTGGTGGGTGGATCGACAAATTGCAGG

Intron 6 GTCCATTAAT ... GTCATGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.62	51.5	27	40.3
A2	33.39	37.5	12	25.2
A3	37.15	33.4	17	22.2
A4	35.21	33.1	12	24.1
A5	32.7	18.5	8	9.8
A6	29.38	98	25	54.6
A7	27.03	164.8	55	66.9
A8	27.86	182.4	38	102
A9	30.55	120.7	31	72.8
A10	25.39	136.8	24	68.7

>TRINITY_DN131381_c0_g1_i11 from sample A1 / Example spliced isoform

TCTGAGGATTCCACGAAGACCATTTCGGGGTCCCGCGTGTTTCATCAACCGAACGCGGTCGGAATCATTGGAGGGGCCTCGGCTCCGGCTATGGCGGATTTCGTCAAAAAAGTCGTTTTGTGGACTTCAGGGGAAACGGAGGAGAGCTTGCCTTTATTGCTCTGCAGCGATCCGCAACTGAAACAAGAGCTCCGTTCGTC
ACTAGCGAGGATCGATCTTGGGACGTCGATGAAATCATTCACCTCAGATCATGGTGGTTCTGTTTTGGCAACACTGAGGGAAAAGAGGACGTTTCTAGAGAGTTCTGGGGCGTGTTGCATTGTGATGCCCTGCCATGTTTCACATGCGTGGTACCAAGAGCTCTCGCAGGGCTGTTCTGTTCCTTTTCTGCATATCGGCG
ACTGCGTGGCGGGAGAGCTCAAGGAGGCGAACTTGAAGCCCATTGAAACGGGAAGCAATGTGAAGATAGGGCTTCTTGGTACAGAGGAGACCTTAAGAGCGGGGTTTTATCAAGATAGGCTTAGCAATCAGGTAAGCTTGCTGTCAATAGTTTTTATATAGGTGCATAATCATAATTTCAGTGTGCTTGAATTGGGTTTC
GAAAAACAGATCAGGATATAGTCAGAAATGCTGTATTTGGGCCTTTACGAATAGCTCATAATTGTTCCAGTGTGGCTGCATCTGGTTTTGGGGACGGGTCGTGGTAATTGATATCTGACAGTTCAACTACAAATTGGTTACCAAGATAGACCATGCCCTACTATGGTAGATTGCAATAGGTCCTCTGCTCCTCATTTATG
GATTTGAAACCTGTTTTATTTGCAGTATAGCAAATTTGTATCGTACATTTTCCTATAGGCTTTTGACAACCGACATATCCAACAAAAGAGGAGGATTACCTTCATACGTGTCGTATATCTCAATGCATACAGCCTTTAAACTACTAGGATTTAATAAGGAATTTATTAAATTCACAATTTTTTGGTTTGCTTTTGGTGAA
GGGCATTGAGGTTCTGGAGTTGGATTTTGTAAAGTTGTCTTTCATAGTTGCAGTTTTACAGATGTTTTTGTGTAAAGTTGCATATTTTCTTTCACCTTCTGTCATAGTTGCAGCCCTTACAGTAGATCTTTTTGTATAGACTCTCCTAATAAAATGATTCATATTGCAAAACAGGACAAACATCGGCTTTCATACATTTG
GGCAAATAATCCGTATGTGAGGAAAGCAATTCATGCTCAGTCGGAGGAGATAACTGGAGAGTGGAAAAGGTGCACAAGCCGCTTTAAGTATATTGAGGAGGTTCACAGTGTAGTTGAATATCATCGCAATCTTACCAGGAAAGGCTACCGTGCTTTAATCTTCAGTGGTGACTATGATATGGTTTTTCCATTCATTGGAA
CTGAAGCATGGATCAGAACATTGAACTATACAATAGTTGATGATTGGAGGTCTTGGTGGGTGGATCGACAAATTGCAGG

>TRINITY_DN131381_c0_g1_i14 from sample A1 / Example unspliced isoform:

CGAAGCGAGGGCCGTAAATGTGATTCCGAGCGTTTAGTCTGCCTGGGATGCGGAGTTTACATGCAGAACGAGCAGCCGATGGTGCCCGGATTCTTCAATAAACCCTCACAGAAAGTTAAAAAGAAAAGAGTGTGGGTTGATGTCAAAGGAGAGAATTTAGGAGATGAAATCTGGCTTAATCCAGACGACAAAAGAGCAAT
TAGTAATCAGATTAAGGTCCAGGAACAGGACCCCAATTTGTCTGAAGAGGGTAAAAATAATGTAAACCCAGAGATAGCTGAAACGCTGGTGGTTTGCGCCCGGTGCCATTCCTTGCGCCACTATGGGAAAGTGAAGGACGAGAGCGTAGAAAATTTGCTGCCGGATTTTGATTTTGAAGTTACAGTGGGAGCCAGATTGA
TGAAAACAACCGGCAGCAGATCGACTGTAGTGATGGTGGTCGATACCGTGGACTTTGACGGGTCTTTTCCTCGGCGTGTGGCGTTGCTGGTGTCGCGTGCGATGGAAGAAGGGGCGCATGTCTGGAAGGAAGGAAAGTCCGGCAACGTTCCCCGTTTAGTGCTCGTTGCCACGAAGCTCGATCTTCTTCCTACTCAGGTT
ACGCCACAGAGGTTAGAGAATTGGGTTCGCCGGCAGTCCAGGTTCGGTGGGGCAGAAAAGTTACATGGAGTTCATCTTGTCAGTTCCCACAAAGGCTGGGGGATTCAGAACTTGGCCGAGCATATCAAGCAGCTGGCCGGTGCTCGAGGGAATGTGTGGGTGATCGGTGCGCAGAATGTGGGCAAATCGACTTTGATTAA
TGCGATTGGGAAGCACGTTGGAGGGAAGGTTACCCAGCTCACCGAAGCTCCGGTTCCAGGCACTACATTGGGGATTCTGAGGATCGAAGGGATTCTGCCTGACAAGGCCAAGTTGTATGACACGCCGGGCCTTCTGCATCCATATCAGATATCAACACGATTGAACAGAGAAGAGCAGAAGATGGTTCAGATTAAAAAAG
AGCTTAGGCCCAGGAGCTACAGGTTAACTGTGGGTCAATGTGTACATGTTGGCGGATTAATGAGGGTTGACATCGAAGAGTCTTCTGTGCAGTCATTCTATGTAACTGTCTGGGCCTCTCCGTTTATTCCCTTGCATATGGGGAGGGTTGAGAATGCGGAAGTGATAAGAGAAAACCATTTCGGACTACAGTTGCAGCCT
CCCATTGGGGTGGAAAGAGTCTCCGAGCTTGGTGAATGGAGGCGAAGGGAATTCAAAGTGTCAGGAAACAGTTGGGATTTCAATTCTATGGATATAGCTACTGCAGGTCTTGGTTGGTTGTCATTTGGCCTGAAAGGTGATGCAACTTTGGCTGTGTGGACTTATGATGGTGTGGATGTAAAGGAACGGAGTGCTTTGAT
CCTCGACAGAGCACGAATCTTTGAAGACACTGGATTCACAGTTTCTAATGTAGTATCCAAGGCGGATAAGAAAAAATCAAATGATGCTAAAGTAAACAAGAAAAGGGATACAAAAAACAAGAAAAGCAAGAATGCAATTGGAGAGATTTGACAACATTGAAGAAATGCCTTGGCCTATAGTTTTTTTTTGAACCGGAAAA
CTCGATAAGGGTCCAAATTATGCAAAATTATGACCACAATTTGCAACAGAGATATGTGGCACACTTAAAATCAAGAATGCATTTGCGCCTTTGTTCCAATGCACCTGCACGGAGTGCAAGCTTCCAAAGTTTCAAAGTGCAACTTTTTGCTCATTCTGCAGTCACAAAACACCAACATCTTATGAATACATTTTATATTT
CAAATTAGAAACAGAGGAGGGCATCATCGAATGCACATTACGAGGATCATAGTTAAAATAATTGTGTCCATTTATTGCAGCTGTGTCATATGAACAATACTGCAAAAGTCTTTCATCAACTATCTTTAATCTTCGCAAAGTCTGGCATCCAGAGATGTACACTCTTGCAATGAACAACCGAATTATTGATGCCAAAAAAT
TCTGAGAAGAAGGAGGAGATAACTGGAGAGTGGAAAAGGTGCACAAGCCGCTTTAAGTATATTGAGGAGGTTCACAGTGTAGTTGAATATCATCGCAATCTTACCAGGAAAGGCTACCGTGCTTTAATCTTCAGgtccattaatacaaatatagttaaatgtctgtgtttacattcatagtttcttgtgattgcgaatac
gactttatgctgacagaattgctatgcatgagcttgtaaatgttcagcatcggatttaactagctttgatgtcatgacagTGGTGACTATGATATGGTTTTTCCATTCATTGGAACTGAAGCATGGATCAGAACATTGAACTATACAATAGTTGATGATTGGAGGTCTTGGTGGGTGGATCGACAAATTGCAGGGTAAGA
CTTGGAGCTCAGAATCCTGGCAGTAGTCTTTAAACA