sp|Q84JP1|NFYA7_ARATH ----> Nuclear transcription factor Y subunit A-7

Intron 1 GTTTCAAGAT ... TTGATTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	57.89	4	4	7
A2	37.93	9	1	10
A3	47.37	10	9	9
A4	100	0	10	7
A5	100	0	4	4
A6	30.43	8	0	7
A7	71.43	2	6	4
A8	100	0	9	5
A9	75	3	12	6
A10	56.52	5	10	3

>TRINITY_DN43877_c0_g3_i3 from sample A7 / Example spliced isoform

TTCACCACAATCCAATAAACAGCACTATGTATATTTTTTTATTGTAGTGAACAATGCACGGAATGGAGAATGCTTTAAAAATTTATATATATTTGAATGCGAGGAGACAAGAAGCCATGCAAGTCAAATATTTTGAAGATTTTCTCACGAGTATCAGGGACCAGCAAAGTCTCGCAGAGGCGGTCAAAGGAAAGATTTTT
GAGGTTACTACTAGAAACACAGCTTTCTGATCTCATTTTGAAGCTTCATAGCTGTGGGCAGTGGCTGCTGTGTGCGTATAGTGGAGGATGGATGAGGGGAAATAATATCGAGTTCTTTTGGGTTCACTCTCCTGAAAGCTGGAGATCATTTGGTGATTTTGCAGGGCTTTTAAGATTTGAGTGATTGACTTTATATTCTC
TGAAGAAAAAAAGCCTGTACAAACAAATTGTTTAAAACAATGGAGAAGACGCCTACACATACTGGAATGCTTTTGTGGAAGTCTTAAGTGTATTATTGTACCAGGCATTGATGGCAGTGCTCC

>TRINITY_DN43877_c0_g3_i2 from sample A7 / Example unspliced isoform:

TTCACCACAATCCAATAAACAGCACTATGTATATTTTTTTATTGTAGTGAACAATGCACGGAATGGAGAATGCTTTAAAAATTTATATATATTTGAATGCGAGGAGACAAGAAGCCATGCAAGTCAAATATTTTGAAGATTTTCTCACGAGTATCAGgtttcaagattctctttaaagagtatcaaggtggtgtagaaag
gaaagcaaactggaaaatttgattacagGGACCAGCAAAGTCTCGCAGAGGCGGTCAAAGGAAAGATTTTTGAGGTTACTACTAGAAACACAGCTTTCTGATCTCATTTTGAAGCTTCATAGCTGTGGGCAGTGGCTGCTGTGTGCGTATAGTGGAGGATGGATGAGGGGAAATAATATCGAGTTCTTTTGGGTTCACTC
TCCTGAAAGCTGGAGATCATTTGGTGATTTTGCAGGGCTTTTAAGATTTGAGTGATTGACTTTATATTCTCTGAAGAAAAAAAGCCTGTACAAACAAATTGTTTAAAACAATGGAGAAGACGCCTACACATACTGGAATGCTTTTGTGGAAGTCTTAAGTGTATTATTGTACCAGGCATTGATGGCAGTGCTCC

Intron 2 GCAGGGTATC ... TGTTAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.41	4	9	12
A2	66.67	6	20	4
A3	56.52	5	11	2
A4	63.64	2	3	4
A5	63.64	2	3	4
A6	64.5	30	65	44
A7	66.67	20	44	36
A8	60.67	35	48	60
A9	63.03	22	36	39
A10	65.22	8	18	12

>TRINITY_DN40163_c0_g1_i6 from sample A6 / Example spliced isoform

CCTTATCTGCACGAATCTCGACATTTGCATGCACTAAAGCGAGCCAGGGGATGTGGAGGGCGGTTTCTCAATGCCAAAAAACAGGATGAAGTGTCACAGGAAAATGATTCAAGTGGTACTATTGTACATGTAGCCAGTGTTGATAAACCTGATGCAGAAAATAAAAGTGCTTCAGTGCAGAACACAGATTCCTAGAGTTC
AAAGCAACAATACTGTAGTATGGCTAGGATATGTTGTTGGCAAGGGGCAGTTTTCTTGAGGGAGCAGTTCTCCCAATAGGTCAAATAGTAATGACTAAAATTTGTGAAATGAGATGTGTCATAGTGCCAAATCTTCATTCCGGTCAGTTTCAGTTGGTAATTCTGAAAGCAGCCCGATTTAAGGGGATATGTATTGGAGC
AAGTTGTTTCTCAGCAAGGATCATCTGTTGAGCATGGGGTTTTCATTGAGTGGAGACGGTGTCATAAAGTAAATGTCAGCGCTCTGTCAACAATTTGATGTTGGATCTATTGTCCCCTTTAGAAGCTGACCTATCGGAGTCCCTTGTTACTGAACTAATTGGTTAAGTTGTAAATAAATAAACTGTACAGTGTCATTTGT
TTCAATTCTATTTTGTAGATGCAAGACGGGGTTGGACTTGGTCCACATGTATTAAAAAGTGAACATATTTTGTGTGCATACAAATTTTTTCCGACATTACCATCTTTAGCTGAACAGTAATATTGCCCATGTTTGCTGTAAGAGATGGCGTCGCCTACAAATTTCGAACCCATGCCACGATTTCAGAGGAATATTTTCTG
CTGTGAAGGGTATCTGCCTGCTCTAGACTCCGTGCTATTTGAAGGTGTTCCATTTACCAGCTTATTCGGTCAAGTATATGCTAATGTGGAGATCTATATAATCTATGTAGATACAACACAATCCCCGTTTTATGTGTATGATTGCTAAAGTTTGATAGACAGTTTGAATGTTCTCACACTTAATTAATTGATAATAAATA
AGTTTAGTCACTTCAGTATTCCCTG

>TRINITY_DN40163_c0_g1_i7 from sample A6 / Example unspliced isoform:

ATGGAGAATGCTTTAAAAATTTATATATATTTGAATGCGAGGAGACAAGAAGCCATGCAAGTCAAATATTTTGAAGATTTTCTCACGAGTATCAGGTTTCAAGATTCTCTTTAAAGAGTATCAAGGTGGTGTAGAAAGGAAAGCAAACTGGAAAATTTGATTACAGATAAGCTAAATAAATCAATGGACTACTTTGACGA
TGAGCTGCCTTGCTATTTCACATTGAAATTTTAATTATTATAGAAATTTATTTTTTGATTAATTAAAAAAGACGGAGTATTTTAAGCATGTAGATAAATGGAAAGCCTAAGCAGATTTAAAGGCTTTGATTGGAGCTTGGAGGTTCTTCCGTATGCGAAATCTTTCTCTTAATTGGCTGATGTGATCCTGTTACCAGGGA
CCAGCAAAGTCTCGCAGAGGCGGTCAAAGGCAGATGAATAGTGATGGGAATCACTGCTTGTAAAAGGACCGTATTCATCGGATATCTCGGGGATTTAGCTTTTTAGCACTCTTGATTCTTTTGAAAGATCATTTATTGAATATCAGTTTATATAGAGGAGTACCACCTCTTTAAAAGCGGAGGGAGGGCATTATTAGAGT
CTTTCAAAAGATTCCCCTCAGTGTTGAGCCCTTCGTTCATGGGAAAAGGCATGAACAACTTCCAGCCAGACAAATATGATAGGTTATGTGCATGTGGTTGCTAAGAAAAGAGAAATGGAAGGAAAGATTTTTGAGGTTACTACTAGAAACACAGCTTTCTGATCTCATTTTGAAGCTTCATAGCTGTGGGCAGTGGCTGC
TGTGTGCGTATAGTGGAGGATGGATGAGGGGAAATAATATCGAGTTCTTTTGGGTTCACTCTCCTGAAAGCTGGAGATCATTTGGTGATTTTGCAGGGCTTTTAAGATTTGAGTGATTGACTTTATATTCTCTGAAGAAAAAAAGCCTGTACAAACAAATTGTTTAAAACAATGGAGAAGACGCCTACACATACTGTTCC
AGGCATTGATGGCAGTGCTCCAGACGAGCAGCAACCTCAGGCTGAACCTGTGCAGCAGGAGGCTCCGCCACCTGGCATGATACCACCACCTGCCATGACTTTACTGCCTCCTGAATATGTAATGCCACACACTCAACTCGAACTAGGACAAGCAATGGTGCGAGCTGCATATCCTTTTCCAGATCCCTACTTTGGAGGCA
TTGTTGCTGCATATGGGCCACAAGCTGTGATTCATCCGCATATGCTTGGGGTTCCACATGCTGGTCTTCCATTGCCATCAGATGCAATTGAGGAGCCTGTTTATGTCAATGCAAAGCAATATAATGGAATTTTGCGACGTCGGCAATCTCGAGCAAAGGCAGAGTCTGAGAACAAACTGATAAAGAACAGAAAGCCTTAT
CTGCACGAATCTCGACATTTGCATGCACTAAAGCGAGCCAGGGGATGTGGAGGGCGGTTTCTCAATGCCAAAAAACAGGATGAAGTGTCACAGGAAAATGATTCAAGTGGTACTATTGTACATGTAGCCAGTGTTGATAAACCTGATGCAGAAAATAAAAGTGCTTCAGTGCAGAACACAGATTCCTAGAGTTCAAAGCA
ACAATACTGTAGTATGGCTAGGATATGTTGTTGGCAAGGGGCAGTTTTCTTGAGGGAGCAGTTCTCCCAATAGGTCAAATAGTAATGACTAAAATTTGTGAAATGAGATGTGTCATAGTGCCAAATCTTCATTCCGGTCAGTTTCAGTTGGTAATTCTGAAAGCAGCCCGATTTAAGGGGATATGTATTGGAGCAAGTTG
TTTCTCAGgcagggtatccgatacctgctttgcctcttcatagttttctatccatattgaaatttgtttattgtgcctcatagctaaaagctaagatgctactttactgattattctttacttaattctcaaataataatcctctataggttaagccatagccaagttactaaatggatgacgactttcttgcatttttg
ttaatcagCAAGGATCATCTGTTGAGCATGGGGTTTTCATTGAGTGGAGACGGTGTCATAAAGTAAATGTCAGCGCTCTGTCAACAATTTGATGTTGGATCTATTGTCCCCTTTAGAAGCTGACCTATCGGAGTCCCTTGTTACTGAACTAATTGGTTAAGTTGTAAATAAATAAACTGTACAGTGTCATTTGTTTCAAT
TCTATTTTGTAGATGCAAGACGGGGTTGGACTTGGTCCACATGTATTAAAAAGTGAACATATTTTGTGTGCATACAAATTTTTTCCGACATTACCATCTTTAGCTGAACAGTAATATTGCCCATGTTTGCTGTAAGAGATGGCGTCGCCTACAAATTTCGAACCCATGCCACGATTTCAGAGGAATATTTTCTGCTGTGA
AGGGTATCTGCCTGCTCTAGACTCCGTGCTATTTGAAGGTGTTCCATTTACCAGCTTATTCGGTCAAGTATATGCTAATGTGGAGATCTATATAATCTATGTAGATACAACACAATCCCCGTTTTATGTGTATGATTGCTAAAGTTTGATAGACAGTTTGAATGTTCTCACACTTAATTAATTGATAATAAATAAGTTTA
GTCACTTCAGTATTCCCTG

Intron 3 GCAGATGAAT ... GAAATGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.67	16.5	2	14
A2	20.54	24	2.3	10
A3	21.65	25.7	5	9
A4	57.69	3.4	4	4
A5	82.28	1	1.3	8
A6	30.03	24.6	9	11
A7	21.12	5.7	1	2
A8	51.78	7.9	7	10
A9	22.63	12.2	2	5
A10	30	7	2	4

>TRINITY_DN110567_c0_g2_i1 from sample A1 / Example spliced isoform

TGTAGGGAGACAAGAAGCCATGCAAGTCAAATATTTTGAAGATTTTCTCACGAGTATCAGGTTTCAAGATTCTCTTTAAAGAGTATCAAGGTGGTGTAGAAAGGAAAGCAAACTGGAAAATTTGATTACAGATAAGCTAAATAAATCAATGGACTACTTTGACGATGAGCTGCCTTGCTATTTCACATTGAAATTTTAAT
TATTATAGAAATTTATTTTTTGATTAATTAAAAAAGACGGAGTATTTTAAGCATGTAGATAAATGGAAAGCCTAAGCAGATTTAAAGGCTTTGATTGGAGCTTGGAGGTTCTTCCGTATGCGAAATCTTTCTCTTAATTGGCTGATGTGATCCTGTTACCAGGGACCAGCAAAGTCTCGCAGAGGCGGTCAAAGGAAAGA

TTTTTGAGGTTACTACTAGAAACACAGCTTTCTGATCTCATTTTGAAGCTTCATAGCTGTGGGCAGTGGCTGCTGTGTGCGTATAGTGGAGGATGGATGAGGGGAAATAATATCGAGTTCTTTTGGGTTCACTCTCCTGAAAGCTGGAGATCATTTGGTGATTTTGCAGGGCTTTTAAGATTTGAGTGATTGACTTTATA
TTCTCTGAAGAAAAAAAGCCTGTACAAACAAATTGTTTAAAACAATGGAGAAGACGCCTACACATACTGGTACATTGGAACTATCCTGGATTTCTTATTTTTTAAGGATTATCCTGTATTTATAGTGTTAACACTATAAAATGGTGGTTCTATACATA

>TRINITY_DN110567_c0_g2_i9 from sample A1 / Example unspliced isoform:

TGTAGGGAGACAAGAAGCCATGCAAGTCAAATATTTTGAAGATTTTCTCACGAGTATCAGGTTTCAAGATTCTCTTTAAAGAGTATCAAGGTGGTGTAGAAAGGAAAGCAAACTGGAAAATTTGATTACAGATAAGCTAAATAAATCAATGGACTACTTTGACGATGAGCTGCCTTGCTATTTCACATTGAAATTTTAAT
TATTATAGAAATTTATTTTTTGATTAATTAAAAAAGACGGAGTATTTTAAGCATGTAGATAAATGGAAAGCCTAAGCAGATTTAAAGGCTTTGATTGGAGCTTGGAGGTTCTTCCGTATGCGAAATCTTTCTCTTAATTGGCTGATGTGATCCTGTTACCAGGGACCAGCAAAGTCTCGCAGAGGCGGTCAAAGgcagat
gaatagtgatgggaatcactgcttgtaaaaggaccgtattcatcggatatctcggggatttagctttttagcactcttgattcttttgaaagatcatttattgaatatcagtttatatagaggagtaccacctctttaaaagcggagggagggcattattagagtctttcaaaagattcccctcagtgttgagcccttcg
ttcatgggaaaaggcatgaacaacttccagccagacaaatatgataggttatgtgcatgtggttgctaagaaaagagaaatggaagGAAAGATTTTTGAGGTTACTACTAGAAACACAGCTTTCTGATCTCATTTTGAAGCTTCATAGCTGTGGGCAGTGGCTGCTGTGTGCGTATAGTGGAGGATGGATGAGGGGAAAT
AATATCGAGTTCTTTTGGGTTCACTCTCCTGAAAGCTGGAGATCATTTGGTGATTTTGCAGGGCTTTTAAGATTTGAGTGATTGACTTTATATTCTCTGAAGAAAAAAAGCCTGTACAAACAAATTGTTTAAAACAATGGAGAAGACGCCTACACATACTGTTCCAGGCATTGATGGCAGTGCTCCAGACGAGCAGCAAC
CTCAGGCTGAACCTGTGCAGCAGGAGGCTCCGCCACCTGGCATGATACCACCACCTGCCATGACTTTACTGCCTCCTGAATATGTAATGCCACACACTCAACTCGAACTAGGACAAGCAATGGTGCGAGCTGCATATCCTTTT

Intron 4 GTTTCAAGAT ... CTGTTACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	53.34	4	5	4.5
A2	36.55	9	3	9
A3	54.08	10	13	11.5
A4	100	0	15	3.5
A5	100	0	4	0
A6	56.41	8	6	16
A7	81.66	2	13	5
A8	100	0	10	4.5
A9	74.61	3	13	5
A10	58.33	5	10	4

>TRINITY_DN43877_c0_g3_i3 from sample A7 / Example spliced isoform

TTCACCACAATCCAATAAACAGCACTATGTATATTTTTTTATTGTAGTGAACAATGCACGGAATGGAGAATGCTTTAAAAATTTATATATATTTGAATGCGAGGAGACAAGAAGCCATGCAAGTCAAATATTTTGAAGATTTTCTCACGAGTATCAGGGACCAGCAAAGTCTCGCAGAGGCGGTCAAAGGAAAGATTTTT
GAGGTTACTACTAGAAACACAGCTTTCTGATCTCATTTTGAAGCTTCATAGCTGTGGGCAGTGGCTGCTGTGTGCGTATAGTGGAGGATGGATGAGGGGAAATAATATCGAGTTCTTTTGGGTTCACTCTCCTGAAAGCTGGAGATCATTTGGTGATTTTGCAGGGCTTTTAAGATTTGAGTGATTGACTTTATATTCTC
TGAAGAAAAAAAGCCTGTACAAACAAATTGTTTAAAACAATGGAGAAGACGCCTACACATACTGGAATGCTTTTGTGGAAGTCTTAAGTGTATTATTGTACCAGGCATTGATGGCAGTGCTCC

>TRINITY_DN43877_c0_g3_i4 from sample A7 / Example unspliced isoform:

TTCACCACAATCCAATAAACAGCACTATGTATATTTTTTTATTGTAGTGAACAATGCACGGAATGGAGAATGCTTTAAAAATTTATATATATTTGAATGCGAGGAGACAAGAAGCCATGCAAGTCAAATATTTTGAAGATTTTCTCACGAGTATCAGgtttcaagattctctttaaagagtatcaaggtggtgtagaaag
gaaagcaaactggaaaatttgattacagataagctaaataaatcaatggactactttgacgatgagctgccttgctatttcacattgaaattttaattattatagaaatttattttttgattaattaaaaaagacggagtattttaagcatgtagataaatggaaagcctaagcagatttaaaggctttgattggagctt
ggaggttcttccgtatgcgaaatctttctcttaattggctgatgtgatcctgttaccagGGACCAGCAAAGTCTCGCAGAGGCGGTCAAAGGCAGATGAATAGTGATGGGAATCACTGCTTGTAAAAGGACCGTATTCATCGGATATCTCGGGGATTTAGCTTTTTAGCACTCTTGATTCTTTTGAAAGATCATTTATTG
AATATCAGTTTATATAGAGGAGTACCACCTCTTTAAAAGCGGAGGGAGGGCATTATTAGAGTCTTTCAAAAGATTCCCCTCAGTGTTGAGCCCTTCGTTCATGGGAAAAGGCATGAACAACTTCCAGCCAGACAAATATGATAGGTTATGTGCATGTGGTTGCTAAGAAAAGAGAAATGGAAGGAAAGATTTTTGAGGTT
ACTACTAGAAACACAGCTTTCTGATCTCATTTTGAAGCTTCATAGCTGTGGGCAGTGGCTGCTGTGTGCGTATAGTGGAGGATGGATGAGGGGAAATAATATCGAGTTCTTTTGGGTTCACTCTCCTGAAAGCTGGAGATCATTTGGTGATTTTGCAGGGCTTTTAAGATTTGAGTGATTGACTTTATATTCTCTGAAGA
AAAAAAGCCTGTACAAACAAATTGTTTAAAACAATGGAGAAGACGCCTACACATACTGGAATGCTTTTGTGGAAGTCTTAAGTGTATTATTGTACCAGGCATTGATGGCAGTGCTCC