sp|Q9SSM4|CHM1B_ARATH ----> ESCRT-related protein CHMP1B

Intron 1 GTAAATGGCT ... TATATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.59	11	11	6
A2	35.48	10	9	2
A3	25.42	22	11	4
A4	37.5	15	11	7
A5	31.25	11	8	2
A6	37.24	75	62	27
A7	34.73	109	96	20
A8	39.79	87	80	35
A9	36.08	93	76	29
A10	33.33	98	61	37

>TRINITY_DN43757_c0_g1_i3 from sample A7 / Example spliced isoform

GCAAGAACGGCCTGAGATTTTTTACTGCATTTGGACTTGGTGGGAACGGCATGAGCTGCGGGGCTTGGAAGGCTGATGGAGAGTTCGAGTCCGCAATCATGTGCAGCCTGCCGCAGGAGGGTTTTGATCTGATCCTCCGGTGTCAACAAAGAAGTGCTTCTGCTGTGACTCTGCAACGAAGTAAGCTTGTTGAATGAGGA
GGAGAATAACTCAATCCAAATGGTGTCCCATTTGGTAGCTGATGCAACAAATGTCAAATCTGCTATCGTAGAGAGAAGCTTAGTTGCATTACAGAACATGACTTCATTACTAACATCTTTCATTATAGCTTTCATTGTGGACTGCTTAAGGTTCATTGAAGTTATTACCGGTTGGATCCATTGTGGGTCTGGAAAGACTG
ACAAAATAAAAATTGGCTATTCAAATGGCTCGAATGACCTCGGTAAATGTTGGAAGAACTTAATTCAGGAGAAGGGCGAGCATTCTTGAAAGTGTTGTTGATGGCTTTAGTTCTATTTAATTATCTCCAATGCTAGTCTGATTATAGCTTCATAGCATGTGTGCACAAGGTAAATCTGGAACCTGTCTGAATTCTTTTGC
TTAAGGTTCAAGAAAATAGATATGTTAAGAGTTTTGCACGTAACAATGTTGTTCCTTTAGATTGTGACAATGGTCCCACCAAATGTCCCATGAAATTTTTTGTAACGGAGGTCTTCCGTAAGCTAGTAAATCAAAGCTAAAAAAGCACACTTCTTGCTTG

>TRINITY_DN43757_c0_g1_i6 from sample A7 / Example unspliced isoform:

GCAAGAACGGCCTGAGATTTTTTACTGCATTTGGACTTGGTGGGAACGGCATGAGCTGCGGGGCTTGGAAGGCTGATGGAGAGTTCGAGTCCGCAATCATGTGCAGCCTGCCGCAGGAGGGTTTTGATCTGATCCTCCGGTGTCAACAAAGAAGTGCTTCTGCTGTGACTCTGCAACGAAGTAAGCTTGTTGAATGAGGA
GGAGAATAACTCAATCCAAATGGTGTCCCATTTGGTAGCTGATGCAACAAATGTCAAATCTGCTATCGTAGAGAGAAGCTTAGTTGCATTACAGAACATGACTTCATTACTAACATCTTTCATTATAGCTTTCATTGTGGACTGgtaaatggctcttcttattctggcaacattccctcttttctctttgcaaattttgt
tgtaaagttaaactttgtcgaaaaagtaaaaattataggttaggaatgcaaagaaaaatcaacgaatatgtgttcatttacgttggagctggtctctgtgctgttgtttcttacatttatcagcaccacttcagcatcgtgggcaagaatctcactgcaacattcgtgaaaaatgaagcttgcaggttgagtgcagttag
cttagatttattttgtctaaagaatcagagaataacatctttttttatcaataaaatgtgtcgaggaatccaagtgtcattcctttatccacatccattgagttaattccaattgagatttcgaggcaattcctagttcatttgtagtcaaaattttcacaatgtgtaaatcctcacaaaagtattttatattgcagCTT
AAGGTTCATTGAAGTTATTACCGGTTGGATCCATTGTGGGTCTGGAAAGACTGACAAAATAAAAATTGGCTATTCAAATGGCTCGAATGACCTCGGTAAATCGAATGACCTCGGTAAATGTTGGAAGAACTTAATTCAGGAGAAGGGCGAGCATTCTTGAAAGTGTTGTTGATGGCTTTAGTTCTATTTAATTATCTCCA
ATGCTAGTCTGATTATAGCTTCATAGCATGTGTGCACAAGGTAAATCTGGAACCTGTCTGAATTCTTTTGCTTAAGGTTCAAGAAAATAGATATGTTAAGAGTTTTGCACGTAACAATGTTGTTCCTTTAGATTGTGACAATGGTCCCACCAAATGTCCCATGAAATTTTTTGTAACGGAGGTCTTCCGTAAGCTAGTAA
ATCAAAGCTAAAAAAGCACACTTCTTGCTTG

Intron 2 GTAAATGGCT ... CATTTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.46	12	11	4
A2	65.99	4.7	9	8.5
A3	38.31	13.7	11	6
A4	40.62	13.1	11	6.9
A5	38.87	8	8.2	2
A6	40.22	70.1	62.3	32
A7	39.67	100.4	97.3	34.7
A8	45.71	82.7	80.4	59
A9	40.45	88.8	76.2	44.5
A10	45.49	56.1	61	32.6

>TRINITY_DN37325_c0_g2_i5 from sample A6 / Example spliced isoform

GCAAGAACGGCCTGAGATTTTTTACTGCATTTGGACTTGGTGGGAACGGCATGAGCTGCGGGGCTTGGAAGGCTGATGGAGAGTTCGAGTCCGCAATCATGTGCAGCCTGCCGCAGGAGGGTTTTGATCTGATCCTCCGGTGTCAACAAAGAAGTGCTTCTGCTGTGACTCTGCAACGAAGTAAGCTTGTTGAATGAGGA
GGAGAATAACTCAATCCAAATGGTGTCCCATTTGGTAGCTGATGCAACAAATGTCAAATCTGCTATCGTAGAGAGAAGCTTAGTTGCATTACAGAACATGACTTCATTACTAACATCTTTCATTATAGCTTTCATTGTGGACTGCACCACTTCAGCATCGTGGGCAAGAATCTCACTGCAACATTCGTGAAAAATGAAGC
TTGCAGCTTAAGGTTCATTGAAGTTATTACCGGTTGGATCCATTGTGGGTCTGGAAAGACTGACAAAATAAAAATTGGCTATTCAAATGGCTCGAATGACCTCGGTAAATGTTGGAAGAACTTAATTCAGGAGAAGGGCGAGCATTCTTGAAAGTGTTGTTGATGGCTTTAGTTCTATTTAATTATCTCCAATGCTAGTC
TGATTATAGCTTCATAGCATGTGTGCACAAGGTAAATCTGGAACCTGTCTGAATTCTTTTGCTTAAGGTTCAAGAAAATAGATATGTTAAGAGTTTTGCACGTAACAATGTTGTTCCTTTAGATTGTGACAATGGTCCCACCAAATGTCCCATGAAATTTTTTGTAACGGAGGTCTTCCGTAAGCTAGTAAATCAAAGCT
AAAAAAGCACACTTCTTGCT

>TRINITY_DN37325_c0_g2_i3 from sample A6 / Example unspliced isoform:

GCAAGAACGGCCTGAGATTTTTTACTGCATTTGGACTTGGTGGGAACGGCATGAGCTGCGGGGCTTGGAAGGCTGATGGAGAGTTCGAGTCCGCAATCATGTGCAGCCTGCCGCAGGAGGGTTTTGATCTGATCCTCCGGTGTCAACAAAGAAGTGCTTCTGCTGTGACTCTGCAACGAAGTAAGCTTGTTGAATGAGGA
GGAGAATAACTCAATCCAAATGGTGTCCCATTTGGTAGCTGATGCAACAAATGTCAAATCTGCTATCGTAGAGAGAAGCTTAGTTGCATTACAGAACATGACTTCATTACTAACATCTTTCATTATAGCTTTCATTGTGGACTGgtaaatggctcttcttattctggcaacattccctcttttctctttgcaaattttgt
tgtaaagttaaactttgtcgaaaaagtaaaaattataggttaggaatgcaaagaaaaatcaacgaatatgtgttcatttacgttggagctggtctctgtgctgttgtttcttacatttatcagCACCACTTCAGCATCGTGGGCAAGAATCTCACTGCAACATTCGTGAAAAATGAAGCTTGCAGGTTGAGTGCAGTTAG
CTTAGATTTATTTTGTCTAAAGAATCAGAGAATAACATCTTTTTTTATCAATAAAATGTGTCGAGGAATCCAAGTGTCATTCCTTTATCCACATCCATTGAGTTAATTCCAATTGAGATTTCGAGGCA

Intron 3 GTTGAGTGCA ... TATATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	34.12	7.8	2	6
A2	59.47	4.6	11	2
A3	24.27	11	3	4
A4	63.69	2.6	2	7
A5	35.43	3.8	2	2
A6	38.8	56	44	27
A7	32.61	66.2	44	20
A8	41.8	66.2	60	35
A9	36.87	64.6	46	29
A10	33.94	69.2	34	37

>TRINITY_DN43757_c0_g1_i1 from sample A7 / Example spliced isoform

GCAAGAACGGCCTGAGATTTTTTACTGCATTTGGACTTGGTGGGAACGGCATGAGCTGCGGGGCTTGGAAGGCTGATGGAGAGTTCGAGTCCGCAATCATGTGCAGCCTGCCGCAGGAGGGTTTTGATCTGATCCTCCGGTGTCAACAAAGAAGTGCTTCTGCTGTGACTCTGCAACGAAGTAAGCTTGTTGAATGAGGA
GGAGAATAACTCAATCCAAATGGTGTCCCATTTGGTAGCTGATGCAACAAATGTCAAATCTGCTATCGTAGAGAGAAGCTTAGTTGCATTACAGAACATGACTTCATTACTAACATCTTTCATTATAGCTTTCATTGTGGACTGCACCACTTCAGCATCGTGGGCAAGAATCTCACTGCAACATTCGTGAAAAATGAAGC

TTGCAGCTTAAGGTTCATTGAAGTTATTACCGGTTGGATCCATTGTGGGTCTGGAAAGACTGACAAAATAAAAATTGGCTATTCAAATGGCTCGAATGACCTCGGTAAATGTTGGAAGAACTTAATTCAGGAGAAGGGCGAGCATTCTTGAAAGTGTTGTTGATGGCTTTAGTTCTATTTAATTATCTCCAATGCTAGTC
TGATTATAGCTTCATAGCATGTGTGCACAAGGTAAATCTGGAACCTGTCTGAATTCTTTTGCTTAAGGTTCAAGAAAATAGATATGTTAAGAGTTTTGCACGTAACAATGTTGTTCCTTTAGATTGTGACAATGGTCCCACCAAATGTCCCATGAAATTTTTTGTAACGGAGGTCTTCCGTAAGCTAGTAAATCAAAGCT
AAAAAAGCACACTTCTTGCTTG

>TRINITY_DN43757_c0_g1_i6 from sample A7 / Example unspliced isoform:

GCAAGAACGGCCTGAGATTTTTTACTGCATTTGGACTTGGTGGGAACGGCATGAGCTGCGGGGCTTGGAAGGCTGATGGAGAGTTCGAGTCCGCAATCATGTGCAGCCTGCCGCAGGAGGGTTTTGATCTGATCCTCCGGTGTCAACAAAGAAGTGCTTCTGCTGTGACTCTGCAACGAAGTAAGCTTGTTGAATGAGGA
GGAGAATAACTCAATCCAAATGGTGTCCCATTTGGTAGCTGATGCAACAAATGTCAAATCTGCTATCGTAGAGAGAAGCTTAGTTGCATTACAGAACATGACTTCATTACTAACATCTTTCATTATAGCTTTCATTGTGGACTGGTAAATGGCTCTTCTTATTCTGGCAACATTCCCTCTTTTCTCTTTGCAAATTTTGT
TGTAAAGTTAAACTTTGTCGAAAAAGTAAAAATTATAGGTTAGGAATGCAAAGAAAAATCAACGAATATGTGTTCATTTACGTTGGAGCTGGTCTCTGTGCTGTTGTTTCTTACATTTATCAGCACCACTTCAGCATCGTGGGCAAGAATCTCACTGCAACATTCGTGAAAAATGAAGCTTGCAGgttgagtgcagttag
cttagatttattttgtctaaagaatcagagaataacatctttttttatcaataaaatgtgtcgaggaatccaagtgtcattcctttatccacatccattgagttaattccaattgagatttcgaggcaattcctagttcatttgtagtcaaaattttcacaatgtgtaaatcctcacaaaagtattttatattgcagCTT
AAGGTTCATTGAAGTTATTACCGGTTGGATCCATTGTGGGTCTGGAAAGACTGACAAAATAAAAATTGGCTATTCAAATGGCTCGAATGACCTCGGTAAATCGAATGACCTCGGTAAATGTTGGAAGAACTTAATTCAGGAGAAGGGCGAGCATTCTTGAAAGTGTTGTTGATGGCTTTAGTTCTATTTAATTATCTCCA
ATGCTAGTCTGATTATAGCTTCATAGCATGTGTGCACAAGGTAAATCTGGAACCTGTCTGAATTCTTTTGCTTAAGGTTCAAGAAAATAGATATGTTAAGAGTTTTGCACGTAACAATGTTGTTCCTTTAGATTGTGACAATGGTCCCACCAAATGTCCCATGAAATTTTTTGTAACGGAGGTCTTCCGTAAGCTAGTAA
ATCAAAGCTAAAAAAGCACACTTCTTGCTTG