NA ----> NA

Intron 1 GTAAGATTAC ... TTTGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.8	179	0	47
A2	23.66	171	0	53
A3	21.46	194	0	53
A4	24.87	145	0	48
A5	22.64	123	0	36
A6	22.64	2183	5	639
A7	23.6	1282	2	396
A8	25.8	1179	3	410
A9	24.67	1310	1	429
A10	25.33	911	0	309

>TRINITY_DN34920_c0_g1_i1 from sample A6 / Example spliced isoform

GTTAATTAATACTTTTGTTTTACCACGTAAACGAGGGTAACTTGGTAAGCAATCTTTAAACTGTCGCAGTAAAAACGGTAATAAAAGTTTTCAGTCAATTGACTCCTAGGGAGGGGCATATGACTGAAAAAGTACACGCGAGCCCGGGTTTTACTCGGTCCAGAATTCATCCCTCCAACTCTATCTGCAAAAAACGAAGA
AAATGGAAATACATATCTATATAAATACGTGTATATCACTGAACGGTGCGCCTACTTTCTTTTGTAATTTTAGGTGTCTTTACGGAATAAAAACATTTGAGTTGAAAAGAGCATCTCGGCGCGACTCGATCTTCGGTCACGTTGTTCTATACACAGCGGCGTTGCCTCTTTTCTGCAGTATTTTTTCGCAGTCCGACTAT
TCTCAGAAAGATGAGTAACCATGGAAAGGGTGCGGAAGGTGTGCCAAAGCTGAGAAAGACTGACGAAAGGAAACCGATGAAACCCGGAGACGAAACTCTGGGATCACGAATAGAGCTTTGCTGCACGGCCGTACAGAAATTCCTTTTCGACGTAGGCACCGGCTCTGAAAGGAGCATACGGGAGGAATGTGGAGATAATC
CCGACACGAGTAAGGCTCTCCGGATGCTGCTGAAGCAAAACAAGGTCAGGCGCTTCGGAACCGGTGGCAGAGCAGATCCGTTTGTGTACGTGGCGGTGGCTACTCCTCAGATGAGTGATCTGGAGAGGCTGAAGCAAGCACGTGCCGTTGGAATCATGTGATTCCATCGGCATGGATTAGCTGGAAATTCCGTCACAATT
CTCTGTGGCGTTTTAAGTTTTCACTTTCAAGATTACCGTGGCGAATTGTGCAATTTGTTGATGCATTTTGTATAGACTCGGAAGAAAATAGGACATGGAATTATTGAAAGCCGGATGCCTCTGTTTTCTTACCCTATTTTGATGGCTTTCTGCTTTGACAAGCTATCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAAC
TTCAGACCAGATTCACTGGAAATTCCGTCACAATTCTCTGTGGTGTTTTAAGTTTTCACTTTCAAGATTACCGTGGCGAATTGTGCAATTTGTTGATGCATTTTGTATAGACTCGGAAGAAAATAGGGCATGGAATTATTGAAAGCCGGATTCCTCTGTTTTCTTACCCTAGTTTGATGGCTTTCTGTTTTGACAAGCTA
TCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGATCCTTCTTTCAATTCCGATGAACAATTTTAATTTTTGGACAAATGAAACTTGTTTTCTGTTCTTTTCGTTCTTTCAATCAATGTGGAGGCTTTTTTAATTCGGTATTTGTTGAATCTGATATTCTGTTTGAAATTCGGTATTTTTTTTAGTTCCGCCGG
TTGTGGTGGCCATTGCAGATAAGTTTATGTATCTGATATAAATTACGCGTATCCTTGCATCAGATATTTGCAAAGATATTCTGTGAGAGAACCCGTATTCTGTGTATGTGGTCATGCCCGTGACGCTGATATTGTTAAATGGTAGGCCATTGTAACAATCCACTTTATTGAATACGAATATTCTTAAGCCACTTTATTGA
ATACGAA

>TRINITY_DN34920_c0_g1_i2 from sample A6 / Example unspliced isoform:

AGCAGATCCGTTTGTGTACGTGgtaagattactttattagcgtccaaattagatactttggataaaatttaacggccgtctcgaattttcgtccggatgttcttgtgtgaatatcggggtttcattgcaggcgtttttcatgtgtttttttttgcctaatttaaaatagataaaattccttcctgtgtgtgcttattttt
gttgcagGCGGTGGCTACTCCTCAGATGAGTGATCTGGAGAGGCTGAAGCAAGCACGTGCCGTTGGAATCATGTGATTCCATCGGCATGGATTAGCTGGAAATTCCGTCACAATTCTCTGTGGCGTTTTAAGTTTTCACTTTCAAGATTACCGTGGCGAATTGTGCAATTTGTTGATGCATTTTGTATAGACTCGGAAGA
AAATAGGACATGGAATTATTGAAAGCCGGATGCCTCTGTTTTCTTACCCTATTTTGATGGCTTTCTGCTTTGACAAGCTATCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGACCAGATTCACTGGAAATTCCGTCACAATTCTCTGTGGTGTTTTAAGTTTTCACTTTCAAGATTACCGTGGCGAATTGTG
CAATTTGTTGATGCATTTTGTATAGACTCGGAAGAAAATAGGGCATGGAATTATTGAAAGCCGGATTCCTCTGTTTTCTTACCCTAGTTTGATGGCTTTCTGTTTTGACAAGCTATCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGATCCTTCTTTCAATTCCGATGAACAATTTTAATTTTTGGACAAAT
GAAACTTGTTTTCTGTTCTTTTCGTTCTTTCAATCAATGTGGAGGCTTTTTTAATTCGGTATTTGTTGAATCTGATATTCTGTTTGAAATTCGGTATTTTTTTTAGTTCCGCCGGTTGTGGTGGCCATTGCAGATAAGTTTATGTATCTGATATAAATTACGCGTATCCTTGCATCAGATATTTGCAAAGATATTCTGTG
AGAGAACCCGTATTCTGTGTATGTGGTCATGCCCGTGACGCTGATATTGTTAAATGGTAGGCCATTGTAACAATCCACTTTATTGAATACGAATATTCTTAAGCCACTTTATTGAATACGAA

Intron 2 GTAAGCCACA ... AATTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.58	195	1	90
A2	30.46	226	2	99
A3	31.88	250	2	117
A4	31.1	206	0	93
A5	27.69	141	0	54
A6	32.16	1605	0	761
A7	32.11	1112	0	526
A8	30.77	756	0	336
A9	28.76	986	0	398
A10	32.71	827	0	402

>TRINITY_DN102273_c0_g1_i1 from sample A2 / Example spliced isoform

AAAAAGTACACGCGAGCCCGGGTTTTACTCGGTCCAGAATTCATCCCTCCAACTCTATCTGCAAAAAACGAAGAAAATGGAAATACATATCTATATAAATACGTGTATATCACTGAACGGTGCGCCTACTTTCTTTTGTAATTTTAGGTGTCTTTACGGAATAAAAACATTTGAGTTGAAAAGAGCATCTCGGCGCGACT
CGATCTTCGGTCACGTTGTTCTATACACAGCGGCGTTGCCTCTTTTCTGCAGTATTTTTTCGCAGTCCGACTATTCTCAGAAAGATGAGTAACCATGGAAAGGGTGCGGAAGGTGTGCCAAAGCTGAGAAAGACTGACGAAAGGAAACCGATGAAACCCGGAGACGAAACTCTGGGATCACGAATAGAGCTTTGCTGCAC
GGCCGTACAGAAATTCCTTTTCGACGTAGGCACCGGCTCTGAAAGGAGCATACGGGAGGAATGTGGAGATAATCCCGACACGAGTAAGGCTCTCCGGATGCTGCTGAAGCAAAACAAGGTCAGGCGCTTCGGAACCGGTGGCAGAGCAGATCCGTTTGTGTACGTGGCGGTGGCTACTCCTCAGATGAGTGATCTGGAGA
GGCTGAAGCAAGCACGTGCCGTTGGAATCATGTGATTCCATCGGCATGGATTAGCTGGAAATTCCGTCACAATTCTCTGTGGCGTTTTAAGTTTTCACTTTCAAGATTACCGTGGCGAATTGTGCAATTTGTTGATGCATTTTGTATAGACTCGGAAGAAAATAGGACATGGAATTATTGAAAGCCGGATGCCTCTGTTT
TCTTACCCTATTTTGATGGCTTTCTGCTTTGACAAGCTATCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGACCAGATTCACTGGAAATTCCGTCACAATTCTCTGTGGCGTTTTAAGTTTTCACTTTCAAGATTACCGTGGCGAATTGTGCAATTTGTTGATGCATTTTGTATAGACTCGGAAGAAAATAG
GGCATGGAATTATTGAAAGCCGGATTCCTCTGTTTTCTTACCCTAGTTTGATGGCTTTCTGTTTTGACAAGCTATCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGATCCTTCTTTCAATTCCGATGAACAATTTTAATTTTTGGACAAATGAAACTTGTTTTCTGTTCTTTTCGTTCTTTCAATCAATGTG
GAGGCTTTTTTAATTCGGTATTTGTTGAATCTGATATTCTGTTTGAAATTCGGTATTTTTTTTAGTTCCGCCGGTTGTGGTGG

>TRINITY_DN102273_c0_g1_i2 from sample A2 / Example unspliced isoform:

TTTCGCAGTCCGACTATTCTCAGgtaagccacaaaaccctttcttgttataaaattcaagtcctatttttccttcgcgttttgggctaattctgttcgggaatttttcagAAAGATGAGTAACCATGGAAAGGGTGCGGAAGGTGTGCCAAAGCTGAGAAAGACTGACGAAAGGAAACCGATGAAACCCGGAGACGAAAC
TCTGGGATCACGAATAGAGCTTTGCTGCACGGCCGTACAGAAATTCCTTTTCGACGTAGGCACCGGCTCTGAAAGGAGCATACGGGAGGAATGTGGAGATAATCCCGACACGAGTAAGGCTCTCCGGATGCTGCTGAAGCAAAACAAGGTCAGGCGCTTCGGAACCGGTGGCAGAGCAGATCCGTTTGTGTACGTGGCGG
TGGCTACTCCTCAGATGAGTGATCTGGAGAGGCTGAAGCAAGCACGTGCCGTTGGAATCATGTGATTCCATCGGCATGGATTAGCTGGAAATTCCGTCACAATTCTCTGTGGCGTTTTAAGTTTTCACTTTCAAGATTACCGTGGCGAATTGTGCAATTTGTTGATGCATTTTGTATAGACTCGGAAGAAAATAGGACAT
GGAATTATTGAAAGCCGGATGCCTCTGTTTTCTTACCCTATTTTGATGGCTTTCTGCTTTGACAAGCTATCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGACCAGATTCACTGGAAATTCCGTCACAATTCTCTGTGGCGTTTTAAGTTTTCACTTTCAAGATTACCGTGGCGAATTGTGCAATTTGTTGA
TGCATTTTGTATAGACTCGGAAGAAAATAGGGCATGGAATTATTGAAAGCCGGATTCCTCTGTTTTCTTACCCTAGTTTGATGGCTTTCTGTTTTGACAAGCTATCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGATCCTTCTTTCAATTCCGATGAACAATTTTAATTTTTGGACAAATGAAACTTGTTT
TCTGTTCTTTTCGTTCTTTCAATCAATGTGGAGGCTTTTTTAATTCGGTATTTGTTGAATCTGATATTCTGTTTGAAATTCGGTATTTTTTTTAGTTCCGCCGGTTGTGGTGG

Intron 3 GTAAGATTAC ... TTTGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.8	179	0	47
A2	23.66	171	0	53
A3	21.46	194	0	53
A4	24.87	145	0	48
A5	22.64	123	0	36
A6	22.64	2183	5	639
A7	23.6	1282	1	396
A8	25.8	1179	2	410
A9	24.67	1310	1	429
A10	25.33	911	0	309

>TRINITY_DN40883_c0_g1_i3 from sample A7 / Example spliced isoform

AAAACATTCACACGACACGCCTCGCCTCGCCTCGAACGCAAAGAATGGGGCTAATAGTAATGGTTAATTAATACTTTTGTTTTACCACGTAAACGAGGGTAACTTGGTGTCTTTACGGAATAAAAACATTTGAGTTGAAAAGAGCATCTCGGCGCGACTCGATCTTCGGTCACGTTGTTCTATACACAGCGGCGTTGCCT
CTTTTCTGCAGTATTTTTTCGCAGTCCGACTATTCTCAGAAAGATGAGTAACCATGGAAAGGGTGCGGAAGGTGTGCCAAAGCTGAGAAAGACTGACGAAAGGAAACCGATGAAACCCGGAGACGAAACTCTGGGATCACGAATAGAGCTTTGCTGCACGGCCGTACAGAAATTCCTTTTCGACGTAGGCACCGGCTCTG
AAAGGAGCATACGGGAGGAATGTGGAGATAATCCCGACACGAGTAAGGCTCTCCGGATGCTGCTGAAGCAAAACAAGGTCAGGCGCTTCGGAACCGGTGGCAGAGCAGATCCGTTTGTGTACGTGGCGGTGGCTACTCCTCAGATGAGTGATCTGGAGAGGCTGAAGCAAGCACGTGCCGTTGGAATCATGTGATTCCAT
CGGCATGGATTAGCTGGAAATTCCGTCACAATTCTCTGTGGCGTTTTAAGTTTTCACTTTCAAGATTACTGTGGCGAATTGTGCAATTTGTTGATGCATTTTGTATAGACTCGGAAGAAAATAGGACATGGAATTATTGAAAGCCGGATGCCTCTGTTTTCTTACCCTATTTTGATGGCTTTCTGCTTTGACAAGCTATC
GTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGACCAGATTCACTGGAAATTCCGTCACAATTCTCTGTGGCGTTTTAAGTTTTCACTTTCAAGATTACTGTGGCGAATTGTGCAATTTGTTGATGCATTTTGTATAGACTCGGAAGAAAATAGGGCATGGAATTATTGAAAGCCGGATTCCTCTGTTTTCTTAC
CCTAGTTTGATGGCTTTCTGTTTTGACAAGCTATCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGATCCTTCTTTCAATTCCGATGAACAATTTTAATTTTTGGACAAATGAAACTTGTTTTCTGTTCTTTTCGTTCTTTCAATCAATGTGGAGGCTTTTTTAATTCGGTATTTGTTGAATCTGATATTCTG
TTTGAAATTCGGTATTTTTTTTAGTTCCGCCGGTTGTGGTGGCCATTGCAGATAAGTTTATGTATCTGATATAAATTACGCGTATCCTTGCATCAGATATTTGCAAAGATATTCTGTGAGAGAACCCGTATTCTGTGTATGTGGTCATGCCCGTGACGCTGATATTGTTAAATGGTAGGCCATTGTAACAATCCACTTTA
TTGAATACGAATATTCTTAAGCCACTTTATTGAATACGAATCTTTTTTTTTTTTCTGATTGCATGTGGGAAGCTTGTATAGTTTTTCAATGATTAATACAAATCCCT

>TRINITY_DN40883_c0_g1_i2 from sample A7 / Example unspliced isoform:

GTTTGTGTACGTGgtaagattactttattagcgtccaaattagatactttggataaaatttaacggccgtctcgaattttcgtccggatgttcttgtgtgaatatcggggtttcattgcaggcgtttttcatgtgttttttttttgcctaatttaaaatagataaaattccttcctgtgtgtgcttatttttgttgcagG
CGGTGGCTACTCCTCAGATGAGTGATCTGGAGAGGCTGAAGCAAGCACGTGCCGTTGGAATCATGTGATTCCATCGGCATGGATTAGCTGGAAATTCCGTCACAATTCTCTGTGGCGTTTTAAGTTTTCACTTTCAAGATTACTGTGGCGAATTGTGCAATTTGTTGATGCATTTTGTATAGACTCGGAAGAAAATAGGA
CATGGAATTATTGAAAGCCGGATGCCTCTGTTTTCTTACCCTATTTTGATGGCTTTCTGCTTTGACAAGCTATCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGACCAGATTCACTGGAAATTCCGTCACAATTCTCTGTGGCGTTTTAAGTTTTCACTTTCAAGATTACTGTGGCGAATTGTGCAATTTGT
TGATGCATTTTGTATAGACTCGGAAGAAAATAGGGCATGGAATTATTGAAAGCCGGATTCCTCTGTTTTCTTACCCTAGTTTGATGGCTTTCTGTTTTGACAAGCTATCGTTTTCTTTTTTTTATTTCGGTTTATAAGAAACTTCAGATCCTTCTTTCAATTCCGATGAACAATTTTAATTTTTGGACAAATGAAACTTG
TTTTCTGTTCTTTTCGTTCTTTCAATCAATGTGGAGGCTTTTTTAATTCGGTATTTGTTGAATCTGATATTCTGTTTGAAATTCGGTATTTTTTTTAGTTCCGCCGGTTGTGGTGGCCATTGCAGATAAGTTTATGTATCTGATATAAATTACGCGTATCCTTGCATCAGATATTTGCAAAGATATTCTGTGAGAGAACC
CGTATTCTGTGTATGTGGTCATGCCCGTGACGCTGATATTGTTAAATGGTAGGCCATTGTAACAATCCACTTTATTGAATACGAATATTCTTAAGCCACTTTATTGAATACGAATCTTTTTTTTTTTTCTGATTGCATGTGGGAAGCTTGTATAGTTTTTCAATGATTAATACAAATCCCT